Abstract: An immunomodulator bioproduct is prepared in a
batch bioprocess with a modified bacterium Pseudomonas
aeruginosa. The bioprocess is performed in 100 L Bioengineering
bioreactor with 42 L cultivation medium made of peptone, meat
extract and sodium chloride. The optimal bioprocess parameters were
determined: temperature – 37 0C, agitation speed - 300 rpm, aeration
rate – 40 L/min, pressure – 0.5 bar, Dow Corning Antifoam M-max.
4 % of the medium volume, duration - 6 hours. This kind of
bioprocesses are appreciated as difficult to control because their
dynamic behavior is highly nonlinear and time varying. The aim of
the paper is to present (by comparison) different models based on
experimental data.
The analysis criteria were modeling error and convergence rate.
The estimated values and the modeling analysis were done by using
the Table Curve 2D.
The preliminary conclusions indicate Andrews-s model with a
maximum specific growth rate of the bacterium in the range of
0.8 h-1.
Abstract: In this paper, a mathematical model of human immunodeficiency
virus (HIV) is utilized and an optimization problem is
proposed, with the final goal of implementing an optimal 900-day
structured treatment interruption (STI) protocol. Two type of commonly
used drugs in highly active antiretroviral therapy (HAART),
reverse transcriptase inhibitors (RTI) and protease inhibitors (PI), are
considered. In order to solving the proposed optimization problem an
adaptive memetic algorithm with population management (AMAPM)
is proposed. The AMAPM uses a distance measure to control the
diversity of population in genotype space and thus preventing the
stagnation and premature convergence. Moreover, the AMAPM uses
diversity parameter in phenotype space to dynamically set the population
size and the number of crossovers during the search process.
Three crossover operators diversify the population, simultaneously.
The progresses of crossover operators are utilized to set the number
of each crossover per generation. In order to escaping the local optima
and introducing the new search directions toward the global optima,
two local searchers assist the evolutionary process. In contrast to
traditional memetic algorithms, the activation of these local searchers
is not random and depends on both the diversity parameters in
genotype space and phenotype space. The capability of AMAPM in
finding optimal solutions compared with three popular metaheurestics
is introduced.
Abstract: Batch fermentation of 5, 10 and 25 g/L biodiesel
derived crude glycerol was carried out at 30, 37 and 450C by
Clostridium pasteurianum cells immobilized on silica. Maximum
yield of 1,3-propanediol (PDO) (0.60 mol/mol), and ethanol (0.26
mol/mol) were obtained from 10 g/L crude glycerol at 30 and 370C
respectively. Maximum yield of butanol (0.28 mol/mol substrate
added) was obtained at 370C with 25 g/L substrate. None of the three
products were detected at 45oC even after 10 days of fermentation.
Only traces of ethanol (0.01 mol/mol) were detected at 450C with 5
g/L substrate. The results obtained for 25 g/L substrate utilization
were fitted in first order rate equation to obtain the values of rate
constant at three different temperatures for bioconversion of glycerol.
First order rate constants for bioconversion of glycerol at 30, 37 and
45oC were found to be 0.198, 0.294 and 0.029/day respectively.
Activation energy (Ea) for crude glycerol bioconversion was
calculated to be 57.62 kcal/mol.
Abstract: Content-based music retrieval generally involves analyzing, searching and retrieving music based on low or high level features of a song which normally used to represent artists, songs or music genre. Identifying them would normally involve feature extraction and classification tasks. Theoretically the greater features analyzed, the better the classification accuracy can be achieved but with longer execution time. Technique to select significant features is important as it will reduce dimensions of feature used in classification and contributes to the accuracy. Artificial Immune System (AIS) approach will be investigated and applied in the classification task. Bio-inspired audio content-based retrieval framework (B-ACRF) is proposed at the end of this paper where it embraces issues that need further consideration in music retrieval performances.
Abstract: Spherical shaped magnetite (Fe3O4) and Au@Fe3O4
nanoparticles were successfully synthesized from Fe electrodes
immersed in water with CTAB surfactant and HAuCl4 solution using
simple method-pulsed plasma in liquid, without the use of dopants or
special conditions for stabilization. Vibrating sample magnetometer
indicated ferromagnetic behavior of particles at room temperature with
coercivity and saturation magnetization of (Hc=105 Oe, Ms=6.83
emu/g) for Fe3O4 and (Hc=175, Ms=3.56emu/g) for Au@Fe3O4
nanoparticles. Structure and morphology of nanoparticles were
characterized by X-ray Diffraction analysis and HR-TEM
measurements. The cytotoxicity of nanoparticles was indicated using a
XTT assay to be very low (cell viability: 98-89% with Fe3O4 and
99-91% for Au@Fe3O4 NPs).
Abstract: Experimental liquid-liquid equilibra of butan-2-ol -
ethanol -water; pentan-1-ol - ethanol - water and toluene - acetone -
water ternary systems were investigated at (25oC). The reliability of
the experimental tie-line data was ascertained by using Othmer-Tobias
and Hand plots. The distribution coefficients (D) and separation
factors (S) of the immiscibility region were evaluated for the three
systems.
Abstract: To fight against the economic crisis, French
Government, like many others in Europe, has decided to give a boost
to high-speed line projects. This paper explores the implementation
and decision-making process in TGV projects, their evolutions,
especially since the Mediterranean TGV-line. This project was
probably the most controversial, but paradoxically represents today a
huge success for all the actors involved.
What kind of lessons we can learn from this experience? How to
evaluate the impact of this project on TGV-line planning? How can
we characterize this implementation and decision-making process
regards to the sustainability challenges?
The construction of Mediterranean TGV-line was the occasion to
make several innovations: to introduce more dialog into the decisionmaking
process, to take into account the environment, to introduce a
new project management and technological innovations. That-s why
this project appears today as an example in terms of integration of
sustainable development.
In this paper we examine the different kinds of innovations
developed in this project, by using concepts from sociology of
innovation to understand how these solutions emerged in a
controversial situation. Then we analyze the lessons which were
drawn from this decision-making process (in the immediacy and a
posteriori) and the way in which procedures evolved: creation of new
tools and devices (public consultation, project management...).
Finally we try to highlight the impact of this evolution on TGV
projects governance. In particular, new methods of implementation
and financing involve a reconfiguration of the system of actors. The
aim of this paper is to define the impact of this reconfiguration on
negotiations between stakeholders.
Abstract: This paper presents a video transmission system using
layered multiple description (coding (MDC) and multi-path transport
for reliable video communications in wireless ad-hoc networks.
The proposed MDC extends a quality-scalable H.264/AVC video
coding algorithm to generate two independent descriptions. The two
descriptions are transmitted over different paths to a receiver in order
to alleviate the effect of unstable channel conditions of wireless adhoc
networks. If one description is lost due to transmission erros,
then the correctly received description is used to estimate the lost
information of the corrupted description. The proposed MD coder
maintains an adequate video quality as long as both description are
not simultaneously lost. Simulation results show that the proposed
MD coding combined with multi-path transport system is largely
immune to packet losses, and therefore, can be a promising solution
for robust video communications over wireless ad-hoc networks.
Abstract: Hemodialysis patients might suffer from unhealthy
care behaviors or long-term dialysis treatments. Ultimately they need
to be hospitalized. If the hospitalization rate of a hemodialysis center
is high, its quality of service would be low. Therefore, how to decrease
hospitalization rate is a crucial problem for health care. In this study
we combined temporal abstraction with data mining techniques for
analyzing the dialysis patients' biochemical data to develop a decision
support system. The mined temporal patterns are helpful for clinicians
to predict hospitalization of hemodialysis patients and to suggest them
some treatments immediately to avoid hospitalization.
Abstract: This paper deals with an on-line identification method
of continuous-time Hammerstein systems by using the radial basis
function (RBF) networks and immune algorithm (IA). An unknown
nonlinear static part to be estimated is approximately represented
by the RBF network. The IA is efficiently combined with the
recursive least-squares (RLS) method. The objective function for the
identification is regarded as the antigen. The candidates of the RBF
parameters such as the centers and widths are coded into binary bit
strings as the antibodies and searched by the IA. On the other hand,
the candidates of both the weighting parameters of the RBF network
and the system parameters of the linear dynamic part are updated
by the RLS method. Simulation results are shown to illustrate the
proposed method.
Abstract: In this paper, the local grid refinement is focused by
using a nested grid technique. The Cartesian grid numerical method is
developed for simulating unsteady, viscous, incompressible flows
with complex immersed boundaries. A finite volume method is used in
conjunction with a two-step fractional-step procedure. The key aspects
that need to be considered in developing such a nested grid solver are
imposition of interface conditions on the inter-block and accurate
discretization of the governing equation in cells that are with the
inter-block as a control surface. A new interpolation procedure is
presented which allows systematic development of a spatial
discretization scheme that preserves the spatial accuracy of the
underlying solver. The present nested grid method has been tested by
two numerical examples to examine its performance in the two
dimensional problems. The numerical examples include flow past a
circular cylinder symmetrically installed in a Channel and flow past
two circular cylinders with different diameters. From the numerical
experiments, the ability of the solver to simulate flows with
complicated immersed boundaries is demonstrated and the nested grid
approach can efficiently speed up the numerical solutions.
Abstract: Following harvest, fresh produce needs to be cooled
immediately in a room where the air temperature and the relative air
humidity are controlled to maintain the produce quality. In this paper,
an experimental study for forced air cooling of fresh produce
(cauliflower) is performed using a pilot developed within our
laboratory. Furthermore, a numerical simulation of spherical
produces, taking into account the aerodynamic aspect and also the
heat transfer in the produce and in the air, was carried out using a
finite element method. At the end of this communication,
experimental results are presented and compared with the simulation.
Abstract: In order to implement flexibility as well as survivable
capacities over passive optical network (PON), a new automatic
random fault-recovery mechanism with array-waveguide-grating
based (AWG-based) optical switch (OSW) is presented. Firstly,
wavelength-division-multiplexing and optical code-division
multiple-access (WDM/OCDMA) scheme are configured to meet the
various geographical locations requirement between optical network
unit (ONU) and optical line terminal (OLT). The AWG-base optical
switch is designed and viewed as central star-mesh topology to
prohibit/decrease the duplicated redundant elements such as fiber and
transceiver as well. Hence, by simple monitoring and routing switch
algorithm, random fault-recovery capacity is achieved over
bi-directional (up/downstream) WDM/OCDMA scheme. When error
of distribution fiber (DF) takes place or bit-error-rate (BER) is higher
than 10-9 requirement, the primary/slave AWG-based OSW are
adjusted and controlled dynamically to restore the affected ONU
groups via the other working DFs immediately.
Abstract: These This paper looks into frameworks which aim at
furthering the discussion of the role of regenerative design practices
in a city-s historic core and the tool of urban design to achieve urban
revitalization on the island of Cyprus. It also examines the region-s
demographic mix, the effectiveness of its governmental coordination
and the strategies of adaptive reuse and strategic investments in older
areas with existing infrastructure. The two main prongs of
investigation will consider the effect of the existing and proposed
changes in the physical infrastructure and fabric of the city, as well as
the catalytic effect of sustainable urban design practices. Through this
process, the work hopes to integrate the contained potential within
the existing historic core and the contributions and participation of
the migrant and immigrant populations to the local economy. It also
examines ways in which this coupling of factors can bring to the front
the positive effects of this combined effort on an otherwise sluggish
local redevelopment effort. The data for this study is being collected
and organized as part of ongoing urban design and development
student workshop efforts in urban planning and design education.
The work is presented in graphic form and includes data collected
from interviews with study area organizations and the community at
large. Planning work is also based on best practices initiated by the
staff of the Nicosia Master Plan task force, which coordinates holistic
planning efforts for the historic center of the city of Nicosia.
Abstract: The purposes of this research were 1) to survey the
number of drugstores that unlawful dispense of asthma prescription
drugs, in form of drug combinations in the Phaya Thai district of
Bangkok, 2) to find the steroids contained in that drug combinations,
3) to find a means for informing general public about the dangers of
drugs and for a campaign to stop dispensing them.
Researcher collected drug combinations from 69 drugstores in
Phaya Thai district from Feb 15, 2012 to Mar 15, 2012. The survey
found 30.43%, 21, drug stores, sold asthma drug combinations to
customers without a prescription. These collected samples were
tested for steroid contamination by using Immunochromatography
kits. Eleven samples, 52.38%, were found contaminated with
steroids. In short, there should be control and inspection of
drugstores in the distribution of steroid medications. To improve the
knowledge of self health maintenance and drug usage among public,
Thai Government and Department of Public Health should educate
people about the side effects of using drug combinations and steroids.
Abstract: A major challenge in camel productivity is the high
mortality rate of camel calves in the early stage due to the lack of
colostrums. This study investigates the time required for the calves to
obtain the optimum amount of the immunoglobulin (IgG). Eleven
pregnant female camels (Camelus Dromedarus) were selected
randomly and variant in age and gestation. After delivery, 7 calves
were obtained and used for this investigation. Colostrum samples
were collected from mothers immediately after parturition. Blood
samples were obtained from the calves as follow: 0 day (before
suckling), 24, 48, 72, 96, 120 and 144 hours, 2nd, 3rd, and 4th weeks
post suckling. Blood serum and colostrums whey were separated and
used to determine IgG concentration, total protein and concentration
of Cortisol and Thyroxin. The results showed high levels of IgG in
camel colostrums (328.8 ± 4.5 mg / ml). The IgG concentration in
serum of calves was the highest within 1st 24 h after suckling (140.75
mg /ml), and then declined gradually reached lower level at 144 h
(41.97 mg / ml). The average turnover rate (t 1/2) of serum IgG in
the all cases was 3.22 days. The turnover of ranged from 2.56 days
for calves have values of IgG more than average and 7.7 days for
those with values below average. In spite of very high levels of
thyroxin in sera of new born the results showed no correlation
between cortisol and thyroxin with IgG levels.
Abstract: This study focuses on examining why the range of
experience with respect to HIV infection is so diverse, especially in
regard to the latency period. An agent-based approach in modelling
the infection is used to extract high-level behaviour which cannot be
obtained analytically from the set of interaction rules at the cellular
level. A prototype model encompasses local variation in baseline
properties, contributing to the individual disease experience, and is
included in a network which mimics the chain of lymph nodes. The
model also accounts for stochastic events such as viral mutations.
The size and complexity of the model require major computational
effort and parallelisation methods are used.
Abstract: Ultra-low-power (ULP) circuits have received
widespread attention due to the rapid growth of biomedical
applications and Battery-less Electronics. Subthreshold region of
transistor operation is used in ULP circuits. Major research challenge
in the subthreshold operating region is to extract the ULP benefits
with minimal degradation in speed and robustness. Process, Voltage
and Temperature (PVT) variations significantly affect the
performance of subthreshold circuits. Designed performance
parameters of ULP circuits may vary largely due to temperature
variations. Hence, this paper investigates the effect of temperature
variation on device and circuit performance parameters at different
biasing voltages in the subthreshold region. Simulation results clearly
demonstrate that in deep subthreshold and near threshold voltage
regions, performance parameters are significantly affected whereas in
moderate subthreshold region, subthreshold circuits are more
immune to temperature variations. This establishes that moderate
subthreshold region is ideal for temperature immune circuits.
Abstract: Mycophenolic acid “MPA" is a secondary metabolite
of Penicillium bervicompactum with antibiotic and
immunosuppressive properties. In this study, fermentation process
was established for production of mycophenolic acid by Penicillium
bervicompactum MUCL 19011 in shake flask. The maximum MPA
production, product yield and productivity were 1.379 g/L, 18.6 mg/g
glucose and 4.9 mg/L.h respectively. Glucose consumption, biomass
and MPA production profiles were investigated during fermentation
time. It was found that MPA production starts approximately after
180 hours and reaches to a maximum at 280 h. In the next step, the
effects of methionine and acetate concentrations on MPA production
were evaluated. Maximum MPA production, product yield and
productivity (1.763 g/L, 23.8 mg/g glucose and 6.30 mg/L. h
respectively) were obtained with using 2.5 g/L methionine in culture
medium. Further addition of methionine had not more positive effect
on MPA production. Finally, results showed that the addition of
acetate to the culture medium had not any observable effect on MPA
production.
Abstract: TiO2/MgO composite films were prepared by coating
the magnesium acetate solution in the pores of mesoporous TiO2
films using a dip coating method. Concentrations of magnesium
acetate solution were varied in a range of 1x10-4 – 1x10-1 M. The
TiO2/MgO composite films were characterized by scanning electron
microscopy (SEM), transmission electron microscropy (TEM),
electrochemical impedance spectroscopy(EIS) , transient voltage
decay and I-V test. The TiO2 films and TiO2/MgO composite films
were immersed in a 0.3 mM N719 dye solution. The Dye-sensitized
solar cells with the TiO2/MgO/N719 structure showed an optimal
concentration of magnesium acetate solution of 1x10-3 M resulting in
the MgO film estimated thickness of 0.0963 nm and giving the
maximum efficiency of 4.85%. The improved efficiency of dyesensitized
solar cell was due to the magnesium oxide film as the wide
band gap coating decays the electron back transfer to the triiodide
electrolyte and reduce charge recombination.