Bioconversion of Biodiesel Derived Crude Glycerol by Immobilized Clostridium pasteurianum: Effect of Temperature
Batch fermentation of 5, 10 and 25 g/L biodiesel
derived crude glycerol was carried out at 30, 37 and 450C by
Clostridium pasteurianum cells immobilized on silica. Maximum
yield of 1,3-propanediol (PDO) (0.60 mol/mol), and ethanol (0.26
mol/mol) were obtained from 10 g/L crude glycerol at 30 and 370C
respectively. Maximum yield of butanol (0.28 mol/mol substrate
added) was obtained at 370C with 25 g/L substrate. None of the three
products were detected at 45oC even after 10 days of fermentation.
Only traces of ethanol (0.01 mol/mol) were detected at 450C with 5
g/L substrate. The results obtained for 25 g/L substrate utilization
were fitted in first order rate equation to obtain the values of rate
constant at three different temperatures for bioconversion of glycerol.
First order rate constants for bioconversion of glycerol at 30, 37 and
45oC were found to be 0.198, 0.294 and 0.029/day respectively.
Activation energy (Ea) for crude glycerol bioconversion was
calculated to be 57.62 kcal/mol.
[1] S. Khanna, A. Goyal, and V.S. Moholkar, "Microbial conversion of
glycerol: present status and future prospects," Critical Reviews in
Biotechnology, doi:10.3109/07388551.2011.604839, 2011.
[2] G.P. da Silva, M. Mack, and J. Contiero, "Glycerol: A promising and
abundant carbon source for industrial microbiology," Biotechnology
Advances, vol. 27, pp. 30-39, 2009.
[3] S.S. Yazdani, and R. Gonzalez, "Anaerobic fermentation of glycerol: a
path to economic viability for the biofuels industry," Current Opinion in
Biotechnology, vol. 18, pp. 213-219, 2007.
[4] P.F.F. Amaral, T.F. Ferreira, G.C. Fontes, and M.A.Z. Coelho, "Glycerol
valorization: New biotechnological routes," Food and Bioproducts
Processing, vol. 87, pp. 179-186, 2009.
[5] R.K. Saxena, P. Anand, S. Saran, and J. Isar, "Microbial production of
1,3-propanediol:Recent developments and emerging opportunities,"
Biotechnology Advances, vol. 27, pp. 895-913, 2009.
[6] D. Hekmat, R. Bauer, and J. Frick, "Optimization of the microbial
synthesis of dihydroxyacetone from glycerol with Gluconobacter
oxydans," Bioprocess and Biosystems Engineering, vol. 26, pp. 109-
116, 2003.
[7] H. Biebl, "Fermentation of glycerol by Clostridium pasteurianum-batch
and continuous culture studies," Journal of Industrial Microbiology and
Biotechnology, vol. 27, pp. 18-26, 2001.
[8] M. Heyndrickx, P.D. Vos, M. Vancanneyt, and J.D. Ley, "The
fermentation of glycerol by Clostridium butyricum LMG 1212t2 and
1213t1 and C .pasteurianum LMG 3285," Applied Microbiology and
Biotechnology, vol. 34, pp. 637-642, 1991.
[9] B. Dabrock, H. Bahl, and G. Gottschalk, "Parameters affecting solvent
production by Clostridium pasteurianum," Applied and Environmental
Microbiology, vol. 58, pp. 1233-1239, 1992.
[10] K.A. Taconi, K.P. Venkataramanan, and D.T. Johnson, "Growth and
solvent production by Clostridium pasteurianum ATCC® 6013™
utilizing biodiesel-derived crude glycerol as the sole carbon source,"
AIChE Environmental Progress and Sustainable Energy, vol. 28, pp.
100-110, 2009.
[11] A. André, P. Diamantopoulou, A. Philippoussis, D. Sarris, M. Komaitis,
and S. Papanikolaou, "Biotechnological conversions of biodiesel
derived waste glycerol into added-value compounds by higher fungi:
production of biomass, single cell oil and oxalic acid," Industrial Crops
and Products, vol. 31, pp. 407-416, 2010.
[12] R.D. Ashby, D.K.Y. Solaiman, and T.A. Foglia, "Synthesis of short-
/medium-chain- length Poly(hydroxyalkanoate) blends by mixed culture
fermentation of glycerol," Biomacromolecules, vol. 6, pp. 2106-2112,
2005.
[13] T. Ito, Y. Nakasimada, K. Senba, T. Matsui, and N. Nishio, "Hydrogen
and Ethanol production from glycerol-containing wastes discharged
after biodiesel manufacturing process," Journal of Bioscience and
Bioengineering, vol. 100, pp. 260-265, 2005.
[14] H. Song, and S.Y. Lee, "Production of succinic acid by bacterial
fermentation," Enzyme and Microbial Technology, vol. 39, pp. 352-
361, 2006.
[15] S. Khanna, A. Goyal, and V.S. Moholkar, " Production of n-butanol
from biodiesel derived crude glycerol using Clostridium pasteurianum
immobilized on amberlite," Fuel, doi:10.1016/j.fuel.2011.10.071, 2011.
[1] S. Khanna, A. Goyal, and V.S. Moholkar, "Microbial conversion of
glycerol: present status and future prospects," Critical Reviews in
Biotechnology, doi:10.3109/07388551.2011.604839, 2011.
[2] G.P. da Silva, M. Mack, and J. Contiero, "Glycerol: A promising and
abundant carbon source for industrial microbiology," Biotechnology
Advances, vol. 27, pp. 30-39, 2009.
[3] S.S. Yazdani, and R. Gonzalez, "Anaerobic fermentation of glycerol: a
path to economic viability for the biofuels industry," Current Opinion in
Biotechnology, vol. 18, pp. 213-219, 2007.
[4] P.F.F. Amaral, T.F. Ferreira, G.C. Fontes, and M.A.Z. Coelho, "Glycerol
valorization: New biotechnological routes," Food and Bioproducts
Processing, vol. 87, pp. 179-186, 2009.
[5] R.K. Saxena, P. Anand, S. Saran, and J. Isar, "Microbial production of
1,3-propanediol:Recent developments and emerging opportunities,"
Biotechnology Advances, vol. 27, pp. 895-913, 2009.
[6] D. Hekmat, R. Bauer, and J. Frick, "Optimization of the microbial
synthesis of dihydroxyacetone from glycerol with Gluconobacter
oxydans," Bioprocess and Biosystems Engineering, vol. 26, pp. 109-
116, 2003.
[7] H. Biebl, "Fermentation of glycerol by Clostridium pasteurianum-batch
and continuous culture studies," Journal of Industrial Microbiology and
Biotechnology, vol. 27, pp. 18-26, 2001.
[8] M. Heyndrickx, P.D. Vos, M. Vancanneyt, and J.D. Ley, "The
fermentation of glycerol by Clostridium butyricum LMG 1212t2 and
1213t1 and C .pasteurianum LMG 3285," Applied Microbiology and
Biotechnology, vol. 34, pp. 637-642, 1991.
[9] B. Dabrock, H. Bahl, and G. Gottschalk, "Parameters affecting solvent
production by Clostridium pasteurianum," Applied and Environmental
Microbiology, vol. 58, pp. 1233-1239, 1992.
[10] K.A. Taconi, K.P. Venkataramanan, and D.T. Johnson, "Growth and
solvent production by Clostridium pasteurianum ATCC® 6013™
utilizing biodiesel-derived crude glycerol as the sole carbon source,"
AIChE Environmental Progress and Sustainable Energy, vol. 28, pp.
100-110, 2009.
[11] A. André, P. Diamantopoulou, A. Philippoussis, D. Sarris, M. Komaitis,
and S. Papanikolaou, "Biotechnological conversions of biodiesel
derived waste glycerol into added-value compounds by higher fungi:
production of biomass, single cell oil and oxalic acid," Industrial Crops
and Products, vol. 31, pp. 407-416, 2010.
[12] R.D. Ashby, D.K.Y. Solaiman, and T.A. Foglia, "Synthesis of short-
/medium-chain- length Poly(hydroxyalkanoate) blends by mixed culture
fermentation of glycerol," Biomacromolecules, vol. 6, pp. 2106-2112,
2005.
[13] T. Ito, Y. Nakasimada, K. Senba, T. Matsui, and N. Nishio, "Hydrogen
and Ethanol production from glycerol-containing wastes discharged
after biodiesel manufacturing process," Journal of Bioscience and
Bioengineering, vol. 100, pp. 260-265, 2005.
[14] H. Song, and S.Y. Lee, "Production of succinic acid by bacterial
fermentation," Enzyme and Microbial Technology, vol. 39, pp. 352-
361, 2006.
[15] S. Khanna, A. Goyal, and V.S. Moholkar, " Production of n-butanol
from biodiesel derived crude glycerol using Clostridium pasteurianum
immobilized on amberlite," Fuel, doi:10.1016/j.fuel.2011.10.071, 2011.
@article{"International Journal of Chemical, Materials and Biomolecular Sciences:53123", author = "Swati Khanna and Arun Goyal and Vijayanand S. Moholkar", title = "Bioconversion of Biodiesel Derived Crude Glycerol by Immobilized Clostridium pasteurianum: Effect of Temperature", abstract = "Batch fermentation of 5, 10 and 25 g/L biodiesel
derived crude glycerol was carried out at 30, 37 and 450C by
Clostridium pasteurianum cells immobilized on silica. Maximum
yield of 1,3-propanediol (PDO) (0.60 mol/mol), and ethanol (0.26
mol/mol) were obtained from 10 g/L crude glycerol at 30 and 370C
respectively. Maximum yield of butanol (0.28 mol/mol substrate
added) was obtained at 370C with 25 g/L substrate. None of the three
products were detected at 45oC even after 10 days of fermentation.
Only traces of ethanol (0.01 mol/mol) were detected at 450C with 5
g/L substrate. The results obtained for 25 g/L substrate utilization
were fitted in first order rate equation to obtain the values of rate
constant at three different temperatures for bioconversion of glycerol.
First order rate constants for bioconversion of glycerol at 30, 37 and
45oC were found to be 0.198, 0.294 and 0.029/day respectively.
Activation energy (Ea) for crude glycerol bioconversion was
calculated to be 57.62 kcal/mol.", keywords = "activation energy, Clostridium pasteurianum, crude
glycerol, immobilization", volume = "6", number = "4", pages = "281-4", }
