An immunomodulator bioproduct is prepared in a
batch bioprocess with a modified bacterium Pseudomonas
aeruginosa. The bioprocess is performed in 100 L Bioengineering
bioreactor with 42 L cultivation medium made of peptone, meat
extract and sodium chloride. The optimal bioprocess parameters were
determined: temperature – 37 0C, agitation speed - 300 rpm, aeration
rate – 40 L/min, pressure – 0.5 bar, Dow Corning Antifoam M-max.
4 % of the medium volume, duration - 6 hours. This kind of
bioprocesses are appreciated as difficult to control because their
dynamic behavior is highly nonlinear and time varying. The aim of
the paper is to present (by comparison) different models based on
experimental data.
The analysis criteria were modeling error and convergence rate.
The estimated values and the modeling analysis were done by using
the Table Curve 2D.
The preliminary conclusions indicate Andrews-s model with a
maximum specific growth rate of the bacterium in the range of
0.8 h-1.
[1] P. M. Doran, "Biotechnol. Bioeng.", 28, p. 73, 1986.
[2] T. B. Young, H. R. Bungay, "Biotechnol. Bioeng.", 15, p. 377, 1973.
[3] A. Moser, "EFB Bioreac. Eng. Course", p.137, 1992.
[4] H. Wucherer, H. Heiler, E. Egerer, "BioEng.", 2, p. 45, 1992.
[5] L. Chen, G. Bastin, V. van Breusegem, "Adaptive Nonlinear Regulation
of Fed-Batch Biological Reactors", Tech. Rep., 1991.
[6] R. Nosrati, C. Fonteix, Marc, "Recents progress en genie des
procedees", Ed. Lavoisier, France, 5, p. 275, 1991
[7] S. A. Freyer, T. V. Kurten, C. Wandrey, "Adv. Biochem. Eng./
Biotechnol, A. Fiechter, (Ed.), 30, p. 780 , 1989
Monod model
Andrews model
Tessier model
Moser model
Fig. 1 The modeling of the correlation between the specific growth rate (╬╝) and the initial substrate concentration
[8] V. Ljubenova, M. Ignatova, "Bioproc. Eng., 11, p. 107, 1994
[9] H. Eiki, T. Osono, "J. Ferm. Bioeng.", 69, p. 313 1990
[10] J. M. Cushing, "Math. Biosc.", 107, p.47, 1991
[11] P. F. Stanbury, A. Whitaker, "Principles of Fermentation Technology",
Pergamon Press, Oxford, 1994
[12] B. G. Turner, D. Ramkrishna, N. B. Jansen, "Biotechnol. Bioeng.", 32,
p. 46, 1998
[13] K. Shimizu, "A tutorial review on bioprocess systems engineering",
Computers chem. Engng. 20, p. 915-941, 1996
[14] H. W. Ryu, M. Kim, J. N. Kim, J. S. Zun, "Appl. Biochem.
Biotechnology", 10, 129-132, 2006.
[1] P. M. Doran, "Biotechnol. Bioeng.", 28, p. 73, 1986.
[2] T. B. Young, H. R. Bungay, "Biotechnol. Bioeng.", 15, p. 377, 1973.
[3] A. Moser, "EFB Bioreac. Eng. Course", p.137, 1992.
[4] H. Wucherer, H. Heiler, E. Egerer, "BioEng.", 2, p. 45, 1992.
[5] L. Chen, G. Bastin, V. van Breusegem, "Adaptive Nonlinear Regulation
of Fed-Batch Biological Reactors", Tech. Rep., 1991.
[6] R. Nosrati, C. Fonteix, Marc, "Recents progress en genie des
procedees", Ed. Lavoisier, France, 5, p. 275, 1991
[7] S. A. Freyer, T. V. Kurten, C. Wandrey, "Adv. Biochem. Eng./
Biotechnol, A. Fiechter, (Ed.), 30, p. 780 , 1989
Monod model
Andrews model
Tessier model
Moser model
Fig. 1 The modeling of the correlation between the specific growth rate (╬╝) and the initial substrate concentration
[8] V. Ljubenova, M. Ignatova, "Bioproc. Eng., 11, p. 107, 1994
[9] H. Eiki, T. Osono, "J. Ferm. Bioeng.", 69, p. 313 1990
[10] J. M. Cushing, "Math. Biosc.", 107, p.47, 1991
[11] P. F. Stanbury, A. Whitaker, "Principles of Fermentation Technology",
Pergamon Press, Oxford, 1994
[12] B. G. Turner, D. Ramkrishna, N. B. Jansen, "Biotechnol. Bioeng.", 32,
p. 46, 1998
[13] K. Shimizu, "A tutorial review on bioprocess systems engineering",
Computers chem. Engng. 20, p. 915-941, 1996
[14] H. W. Ryu, M. Kim, J. N. Kim, J. S. Zun, "Appl. Biochem.
Biotechnology", 10, 129-132, 2006.
@article{"International Journal of Medical, Medicine and Health Sciences:53143", author = "Mihai Caramihai and Irina Severin and Ana Aurelia Chirvase and Adrian Onu and Cristina Tanase and Camelia Ungureanu", title = "Therapeutic Product Preparation Bioprocess Modeling", abstract = "An immunomodulator bioproduct is prepared in a
batch bioprocess with a modified bacterium Pseudomonas
aeruginosa. The bioprocess is performed in 100 L Bioengineering
bioreactor with 42 L cultivation medium made of peptone, meat
extract and sodium chloride. The optimal bioprocess parameters were
determined: temperature – 37 0C, agitation speed - 300 rpm, aeration
rate – 40 L/min, pressure – 0.5 bar, Dow Corning Antifoam M-max.
4 % of the medium volume, duration - 6 hours. This kind of
bioprocesses are appreciated as difficult to control because their
dynamic behavior is highly nonlinear and time varying. The aim of
the paper is to present (by comparison) different models based on
experimental data.
The analysis criteria were modeling error and convergence rate.
The estimated values and the modeling analysis were done by using
the Table Curve 2D.
The preliminary conclusions indicate Andrews-s model with a
maximum specific growth rate of the bacterium in the range of
0.8 h-1.", keywords = "bioprocess modeling, Pseudomonas aeruginosa, kinetic models,", volume = "4", number = "6", pages = "248-4", }