Abstract: There are various sources of energies available
worldwide and among them, crude oil plays a vital role. Oil recovery
is achieved using conventional primary and secondary recovery
methods. In-order to recover the remaining residual oil, technologies
like Enhanced Oil Recovery (EOR) are utilized which is also known
as tertiary recovery. Among EOR, Microbial enhanced oil recovery
(MEOR) is a technique which enables the improvement of oil
recovery by injection of bio-surfactant produced by microorganisms.
Bio-surfactant can retrieve unrecoverable oil from the cap rock which
is held by high capillary force. Bio-surfactant is a surface active agent
which can reduce the interfacial tension and reduce viscosity of oil
and thereby oil can be recovered to the surface as the mobility of the
oil is increased. Research in this area has shown promising results
besides the method is echo-friendly and cost effective compared with
other EOR techniques. In our research, on laboratory scale we
produced bio-surfactant using the strain Pseudomonas putida (MTCC
2467) and injected into designed simple sand packed column which
resembles actual petroleum reservoir. The experiment was conducted
in order to determine the efficiency of produced bio-surfactant in oil
recovery. The column was made of plastic material with 10 cm in
length. The diameter was 2.5 cm. The column was packed with fine
sand material. Sand was saturated with brine initially followed by oil
saturation. Water flooding followed by bio-surfactant injection was
done to determine the amount of oil recovered. Further, the injection
of bio-surfactant volume was varied and checked how effectively oil
recovery can be achieved. A comparative study was also done by
injecting Triton X 100 which is one of the chemical surfactant. Since,
bio-surfactant reduced surface and interfacial tension oil can be easily
recovered from the porous sand packed column.
Abstract: The present investigation deals with bioleaching of spent petroleum catalyst using At. ferrooxidans and At. thiooxidans. The spent catalyst used in the present study was pretreated with acetone to remove the oily hydrocarbons. FESEM and XPS analysis indicated the presence of metals in sulfide and oxide forms in spent catalyst. Both At. ferrooxidans and At. thiooxidans were found to be highly effective in producing the acid. Bioleaching with At. ferrooxidans and At. thiooxidans led to higher recovery of metals compare to control. During bioleaching similar recoveries of metals were obtained using At. ferrooxidans and At. thiooxidans. This might be due to the presence of metals as soluble oxides and sulphides in the spent catalyst. At the end of bioleaching, about 87-90% Ni, 34% Al, 65-73% Mo and 92-97% V were leached using above bacteria. It is elucidated that bioleaching with At. thiooxidans is comparatively more advantageous due to lower cost of sulphur.
Abstract: This paper presents the coordinated controller design of static synchronous compensator (STATCOM) and power system stabilizers (PSSs) for power system stability improvement. Coordinated design problem of STATCOM-based controller with multiple PSSs is formulated as an optimization problem and optimal controller parameters are obtained using bacteria foraging optimization algorithm. By minimizing the proposed objective function, in which the speed deviations between generators are involved; stability performance of the system is improved. The nonlinear simulation results show that coordinated design of STATCOM-based controller and PSSs improve greatly the system damping oscillations and consequently stability improvement.
Abstract: A total of 6 isolates of Bacillus subtilis were isolated from oil mill waste collected in Namakkal district, Tamilnadu, India. The isolated bacteria were screened using lipase screening medium containing Tween 80. BS-3 isolate exhibited a greater clear zone than the others, indicating higher lipase activity. Therefore, this isolate was selected for media optimization studies. Ten process variables were screened using Plackett–Burman design and were further optimized by central composite design of response surface methodology for lipase production in submerged fermentation. Maximum lipase production of 16.627 U/min/ml were predicted in medium containing yeast extract (9.3636g), CaCl2 (0.8986g) and incubation periods (1.813 days). A mean value of 16.98 ± 0.2286 U/min/ml of lipase was acquired from real experiments.
Abstract: Changes in the sensory, chemical and microbiological quality of the Mediterranean hake during post-catch handling and distribution were investigated. 115 fish samples were seasonally received during three stages of the transfer route from the sea to the consumer and two storage methods were recorded, seawater and ice storage. Microbiological evaluation revealed higher status for the ice stored samples regarding heterotrophic bacteria (2.68 log cfu/g and 1.92 log cfu/g at 22oC and 37°C respectively) and psychrotrophic counts (3.20 log cfu/g), with statistically significant differences among storage methods. Sensory evaluation also revealed higher status for the ice stored samples with a mean quality index of 0.17 and a spoilage time estimated at 30 hours, in contrast to seawater storage, which varied from 0.28 to 0.3, and a 14-hour estimated spoilage. Detected pathogens were identified mainly in the seawater stored samples, posing questions on the quality of the product reaching the seafood markets.
Abstract: Chitosan is a derivative of chitin, a compound usually
isolated from the shells of some crustaceans such as crab, lobster and
shrimp. It has biocompatible, biodegradable, and antimicrobial
properties. To use these properties of chitosan in biomedical fields,
chitosan films (1%, 2%, 3% and 4%) were prepared by using l%
lactic acid as solvent. The effects of chitosan films on tensile
strength, elongation at break, degree of swelling, thickness,
morphology, allergic and irritation reactions and antibacterial
property were evaluated. Staphylococcus aureus and Escherichia coli
were used as tested microorganisms. In vivo wound healing activities
of chitosan films were investigated using mice model. As results,
Chitosan films have similar appearance and good swelling properties
and 4% chitosan film showed the better swelling activity and the
greatest elongation ratio than the other chitosan films. They also
showed their good activity of wound healing in mice model.
Moreover, the results showed that the films did not produce any
unwilling symptoms (allergy or irritation). In conclusion, it is evident
that the chitosan film has the potentiality to use as wound healing
biofilms in the biomedical fields.
Abstract: The degrading effect due to bacterial growth on the structural integrity of concrete floor surfaces is predictable; this consequently cause development of surface micro cracks in which organisms penetrate through resulting in surface spalling. Hence, the need to develop mix design meeting the requirement of floor surfaces exposed to aggressive agent to improve certain material properties with good workability, extended lifespan and low cost is essential. In this work, tests were performed to examine the microbial activity on kitchen floor surfaces and the effect of adding admixtures. The biochemical test shows the existence of microorganisms (E.coli, Streptococcus) on newly casted structure. Of up to 6% porosity was reduced and improvement on structural integrity was observed upon adding mineral admixtures from the concrete mortar. The SEM result after 84 days of curing specimens, shows that chemical admixtures have significant role to enable retard bacterial penetration and good quality structure is achieved.
Abstract: Cyanobacteria play a vital role in the production of phycobiliproteins that includes phycocyanin and phycoerythrin pigments. Phycocyanin and related phycobiliproteins have wide variety of application that is used in the food, biotechnology and cosmetic industry because of their color, fluorescent and antioxidant properties. The present study is focused to understand the pigment at molecular level in the Cyanobacteria Oscillatoria terebriformis NTRI05 and Oscillatoria foreaui NTRI06. After extraction of genomic DNA, the amplification of C-Phycocyanin gene was done with the suitable primer PCβF and PCαR and the sequencing was performed. Structural and Phylogenetic analysis was attained using the sequence to develop a molecular model.
Abstract: In this study, statistical optimization design was used to study the optimum disinfection parameters using defatted crude Moringa oleifera seed extracts against Escherichia coli (E. coli) bacterial cells. The classical one-factor-at-a-time (OFAT) and response surface methodology (RSM) was used. The possible optimum range of dosage, contact time and mixing rate from the OFAT study were 25mg/l to 200mg/l, 30minutes to 240 minutes and 100rpm to 160rpm respectively. Analysis of variance (ANOVA) of the statistical optimization using faced centered central composite design showed that dosage, contact time and mixing rate were highly significant. The optimum disinfection range was 125mg/l, at contact time of 30 minutes with mixing rate of 120 rpm.
Abstract: No negative control nor control to prevent microbes from escaping was set when the S-shaped flask experiments were performed by Pasteur. Microscope was not used to observe the media in the flasks. Louis Pasteur’s S-shaped flask experiment was re-examined by using U-shaped flasks, modified S-shaped flasks and microscope. A mixture of microbes was isolated from the room air, from which one rod-shaped Bacillus species with proposed name Bacillus gaso-mobilis sp nov and one grape-shaped Staphylococcus species with proposed name of Staphylococcus gaso-mobilis sp nov were identified. Their penicillin and ampicillin resistant strains containing plasmids were isolated. These bacteria could change color, produce odor and automatically move in the air. They did not form colonies on solid media. They had a high suspension capacity in liquid media. Their light absorbance peaked at the wave length of 320 nm. It was concluded that there were flaws with Louis Pasteur’s S-shaped flask experiments.
Abstract: One of the major pollutants in the environment is arsenic (As). Due to the toxic effects of As to all organisms, its remediation is necessary. Conventional technologies used in the remediation of As contaminated soils are expensive and may even compromise the structure of the soil. An attractive alternative is phytoremediation, which is the use of plants which can take up the contaminant in their tissues. Plant growth promoting bacteria (PGPB) has been known to enhance growth of plants through several mechanisms such as phytohormone production, phosphate solubilization, siderophore production and 1-aminocyclopropane-1- carboxylate (ACC) deaminase production, which is an essential trait that aids plants especially under stress conditions such as As stress. Twenty one bacteria were isolated from As-contaminated soils in the vicinity of the Janghang Smelter in Chungnam Province, South Korea. These exhibited high tolerance to either arsenite (As III) or arsenate (As V) or both. Most of these isolates possess several plant growth promoting traits which can be potentially exploited to increase phytoremediation efficiency. Among the identified isolates is Pseudomonas sp. JS1215, which produces ACC deaminase, indole acetic acid (IAA), and siderophore. It also has the ability to solubilize phosphate. Inoculation of JS1215 significantly enhanced root and shoot length and biomass accumulation of maize under normal conditions. In the presence of As, particularly in lower As level, inoculation of JS1215 slightly increased root length and biomass. Ethylene increased with increasing As concentration, but was reduced by JS1215 inoculation. JS1215 can be a potential bioinoculant for increasing phytoremediation efficiency.
Abstract: Probiotic bacteria especially Lactobacillus spp. and Bifidobacterium exert suppressive effect on Helicobacter pylori. Cinnamon and licorice have been traditionally used for the treatment of gastric ulcer. The objectives of this study were to determine the effects of herbs on yogurt fermentation, the level of probiotic bacteria in yogurt during 28 days storage and the effect of herbal yogurt on the growth of H. pylori in vitro. Cinnamon or licorice was mixed with milk and the mixture was fermented with probiotic bacteria to form herbal-yogurt. Changes of pH and total titratable acids were monitored and the viability of probiotic bacteria was evaluated during and after refrigerated storage. The in vitro inhibition of H. pylori growth was determined using agar diffusion and minimum inhibitory concentration (MIC) method. The presence of herbs did not affect the probiotic population during storage. There were no significant differences in pH and TTA between herbal-yogurts and plain-yogurt during fermentation and storage. Water extract of cinnamon-yogurt showed the highest inhibition effect (13.5mm) on H. pylori growth in comparison with licorice-yogurt (11.2mm). The present findings indicate cinnamon and licorice has bioactive components to decrease the growth of H. pylori.
Abstract: Safe drinking water is one of the biggest issues facing
the planet this century. The primary aim of this paper is to present our
research focused on theoretical and experimental analysis of potable
water and in-building water distribution systems from the point of
view of microbiological risk on the basis of confrontation between
the theoretical analysis and synthesis of gathered information in
conditions of the Slovak Republic. The presence of the bacteria
Legionella in water systems, especially in hot water distribution
system, represents in terms of health protection of inhabitants the
crucial problem which cannot be overlooked. Legionella
pneumophila discovery, its classification and its influence on
installations inside buildings are relatively new. There are a lot of
guidelines and regulations developed in many individual countries for
the design, operation and maintenance for tap water systems to avoid
the growth of bacteria Legionella pneumophila, but in Slovakia we
don-t have any. The goal of this paper is to show the necessity of
prevention and regulations for installations inside buildings verified
by simulation methods.
Abstract: Microbial air contamination of the outdoor air in Marine Durres-s Harbour (Durres, Albania) was estimated by sedimentation technique in August-October 2008. The sampling areas were: Ferry Terminal (FT), Fishery Harbor (FH), East Zone (EZ), Fuel Quay (FQ) and Apollonian Beach (AB). The aim of this study was to measure the number of aerobic plate count (mesophilic aerobic bacteria) and fungi (yeasts and molds) in the outdoor air in these areas. The number of colonies that were formed determines the number of cells at the moment in the outdoor air; respectively the number of mesophilic aerobic bacteria and yeasts and molds. The measure of bacteria and fungi used is CFU (Colony Forming Units) per Petri dish. It is said that marine harbours are very polluted areas. The aim of study was the definition of mesophilic aerobic bacteria and yeasts and molds number, and the comparison of microorganisms number in air sampling areas.
Abstract: Studies were carried out on the comparative study of the production of Avicelase enzyme using sugarcane bagasse-SCB in two different statuses (i.e. treated and untreated SCB) by thermophilic Geobacillus stearothermophilus at 50ºC. Only four thermophilic bacterial isolates were isolated and assayed for Avicelase production using UntSCB and TSCB. Only one isolate selected as most potent and identified as G. stearothermophilus used in this study. A specific endo-β-1,4-D-glucanase (Avicelase EC 3.2.1.91) was partially purified from a thermophilic bacterial strain was isolated from different soil samples when grown on cellulose enrichment SCB substrate as the sole carbon source. Results shown that G. stearothermophilus was the better Avicelase producer strain. Avicelase had an optimum pH and temperature 7.0 and 50ºC for both UntSCB and TSCB and exhibited good pH stability between "5-8" and "4-9", however, good temperature stability between (30-80ºC) for UntSCB and TSCB, respectively. Other factors affecting the production of Avicelase were compared (i.e. SCB concentration, inoculum size and different incubation periods), all results observed and obtained were revealed that the TSCB was exhibited maximal enzyme activity in comparison with the results obtained from UntSCB, so, the TSCB was enhancing the Avicelase production.
Abstract: Tufting carpet is a very suitable substrate for growing
microorganism such as pathogenic microbes, due to the direct touch
with human body, long washing periods and laying on the floor; in
fact there are 3 major problems: To risk human health, Prepare bad
odors and Destruction of the products.. In the presented research, for
investigation of presence most common microbes on polyester
tufting, first goods laid in a public place (in the corridor fair) for 30
days and the existence of some microbes were investigate on it with
two methods of enrichment in nutrient environments such as
thioglycolate and noutrunt brath, and shake the dust off the polyester
tufting onto cultivation mediums such as blood agar and noutrunt
agar. After the microorganism colonics are grown, the colonies were
separated and six microbial tests such as cataloes and sitrat were
carried out in five phases on the colonics for identifying the varieties
of bacteria. As a result of tests, 5 type of bacteria, such as
Escherichia coli, staphylococcus saprophytic as were identified. Each
of the mentioned bacteria can be seriously harmful for the heath of
human.
Abstract: Novel nitrogen removal technologies via nitrite
pathway attract increasing interest in recent years. In this study,
batch experiments were performed to investigate nitrite accumulation
characteristics and shifts in nitrifying community structure at
different growth environments including ammonia concentration, pH
and alkalinity. It was found that nitrite accumulation ratios were
maintained at around 95% at studied conditions, and the optimum pH
and Alk/N (ratio between alkalinity and nitrogen) for ammonium
oxidization were 8.5 and 8.33, respectively. Fluorescence in situ
hybridization analysis of nitrifying bacteria showed that high free
ammonia (from influent ammonium or caused by high pH)
significantly altered the structure of nitrifying community, leading to
abundance of ammonia-oxidizing bacteria (AOB), especially
Nitrososmonas, and inhibition of nitrite-oxidizing bacteria (NOB).
The results suggest that free ammonia plays more important role than
other studied conditions on nitrite accumulation.
Abstract: In this study, static batch fermentation was used for bacterial cellulose production in date syrup solution (Bx. 10%) at 28°C using Gluconacetobacter. xylinus (PTCC 1734). The physicochemical properties of standard Sigma CMC and the produced carboxymethyl bacterial cellulose (CMBC) were studied using FT-IR spectroscopy, X-ray diffractometry (XRD) and Scanning Electron Microscopy (SEM). According to the FT-IR spectra the bands at 1664 and 1431 cm-1 indicate that carboxylic acid groups and carboxylate groups exist on the surface. The SEM imaging of CMBC and CMC carried out in magnification of 1K. Comparing the SEM imaging obviously showed that the ribbon shape in CMC remained but the length of ribbons became shorter while that shape changed to flake shape for CMBC. Determination of the area under XRD patterns demonstrated that the crystallinity amount of CMC was more than that for CMBC (51.08% and 81.84% for CMBC and CMC, respectively).
Abstract: In this study the extracts of the Iraqi herb Tribulus
terrestris (Al-Hassage or Al-Kutub) was done by using of polar and
non polar solvents, then the biological activity of these extractants
was studied in three fields, First, the antibacterial activity (in vitro)
on gram positive bacteria (Staphylococcus aureus), and gram
negative bacteria (E. coli, Proteus vulgaris, Pseudomonas
aerugiuosa, and Klebsiella), all extracts showed considerable activity
against all bacteria. Second, the effect of extracts on free serum
testosterone level in male mice (in vivo), the alcoholic, and
acetonitrilic extracts showed significant (P < 0.05) increase in free
serum testosterone level, and we found that the extracts contained
compounds with less genotoxic effects in mice germ cells. 3rd, was to
study the effect of methanolic extract of T. terrestris in diabetes
management.
Abstract: Ever since industrial revolution began, our ecosystem
has changed. And indeed, the negatives outweigh the positives.
Industrial waste usually released into all kinds of body of water, such
as river or sea. Tempeh waste is one example of waste that carries
many hazardous and unwanted substances that will affect the
surrounding environment. Tempeh is a popular fermented food in
Asia which is rich in nutrients and active substances. Tempeh liquid
waste- in particular- can cause an air pollution, and if penetrates
through the soil, it will contaminates ground-water, making it
unavailable for the water to be consumed. Moreover, bacteria will
thrive within the polluted water, which often responsible for causing
many kinds of diseases. The treatment used for this chemical waste is
biological treatment such as constructed wetland and activated
sludge. These kinds of treatment are able to reduce both physical and
chemical parameters altogether such as temperature, TSS, pH, BOD,
COD, NH3-N, NO3-N, and PO4-P. These treatments are implemented
before the waste is released into the water. The result is a
comparation between constructed wetland and activated sludge,
along with determining which method is better suited to reduce the
physical and chemical subtances of the waste.