Study the Biological Activities of Tribulus Terrestris Extracts
In this study the extracts of the Iraqi herb Tribulus
terrestris (Al-Hassage or Al-Kutub) was done by using of polar and
non polar solvents, then the biological activity of these extractants
was studied in three fields, First, the antibacterial activity (in vitro)
on gram positive bacteria (Staphylococcus aureus), and gram
negative bacteria (E. coli, Proteus vulgaris, Pseudomonas
aerugiuosa, and Klebsiella), all extracts showed considerable activity
against all bacteria. Second, the effect of extracts on free serum
testosterone level in male mice (in vivo), the alcoholic, and
acetonitrilic extracts showed significant (P < 0.05) increase in free
serum testosterone level, and we found that the extracts contained
compounds with less genotoxic effects in mice germ cells. 3rd, was to
study the effect of methanolic extract of T. terrestris in diabetes
management.
[1] R. A. Mothana, and U. Lindequist. Antimicrobial activity of some
medicinal plants of the island Soqotra. J. Ethnopharmacol., 2005, 96(1-
2):177-181.
[2] K, Abeywickrama, and G. A. Bean. Toxigenic Aspergillus flavus and
aflatoxins in Sri Lankan medicinal plant material. Mycopa-thologia.,
1991, 113: 187-190.
[3] K. Gauthaman, A. P. Ganesan, and R. N. Prasad. "Sexual effects of
puncturevine (Tribulus terrestris) extract (protodioscin): an evaluation
using a rat model". Journal of Alternative and Complementary
Medicine., 2003, 9 (2): 257-265.
[4] S. H. Majeed, and M. J. Mahmood. Herbs and Medicinal Plants in Iraq
between Traditional Medicine and Scientific Research. 1st Ed. Baghdad:
Dar Al-Thaowra for Publishing., 1988, p. 40. (in Arabic).
[5] S. Aldein. Medicinal Herbs. 1st Ed. Baghdad: Dar Al-Shoun Al-
Thaqafia Al-Aama for Publishing.1986, p. 70. (in Arabic).
[6] M. M. Cowan. Plant products as antimicrobial agents. Clin.
Microbiol. Rev. 1999, 12(4): 564-582.
[7] O. Kandil, N. M. Radwan, A. B. Hassan, A. M. Amer, and H. A. El-
Banna. Extracts and fractions of Thymus capitatus exhibit antimicrobial
activities. J Ethnopharmacol. 1994, 44(1):19-24.
[8] D. McCan, L. Kirkis. Evaluation of Free Testosterone in serum. J Clin
Immunoassay. 1985, 8: 234-6.
[9] M. R. Atlas, E. Alfres, and C. Lawrence "Laboratory Manual
Experimental Microbiology" Mosby- Year Book, Inc. 1995.
[10] E. Evans, G. Breckon, and C. Ford.Air drying method for meiotic
preparation from mammalian testes. Cytogenetics 1964, 3: 284-294.
[11] E. Bedir, A. Khan. New steroidal glycosides from the fruits of Tribulus
terrestris. Pharmazie 2000, 57:491-3.
[12] Y. Wan, K. Ohtani, R. Kasai, and K. Yamasaki, Steroidal saponins from
fruits of Tribulus terrestris. Phytochemistry 1997, 45:811-7.
[13] A. G. Brown, M. D. Vukovich, E. R. Martini, L. M. Kohut, W. D. Frank,
DA Jackson, et al . Endocrine and lipid responses to chronic
androstenediol-herbal supplementation in 30 to 58 year old men. J Am
Coll Nutr 2002, 20:520-8.
[14] K. M. Arsyad. Effect of protodioscin on the quality and quantity of
sperms from males with moderate idiopathic oligozoospermia. Medica
1996, 22:614-8.
[15] S. K. Bhattacharya. National conference on Recent trends in Spice and
Medicinal Plants Research. Calcutta. India: April 1998, p. 652-4.
[16] B. Sun, W. Qu, and Z. Bai. The inhibitory effect of saponins from
Tribulus terrestris on Bcap-37 breast cancer line in vitro. Zhong Yao
Cai 2003, 26:104-6.
[17] Castoldi, G.; Scapoli, P.; and Spanedda, R. (1969). Chromosomes and
chloramphenicol. Arch. Ital. Patol. Clin. Tumori, 12:117-141.
[18] E.H. Al-Neimy, Biological effects of Capparis spinosa and Rumex
acetosella extracts on animal and human normal and tumor cells. Ph.D.
thesis, college of science, 2007, Al-Nahrain University.
[19] R. Chatterjee, G. A. Haines, D. M. Perera, A. Goldston, and L. D.
Morris. Testicular and sperm DNA damage after treatment with
fludarabin for chronic lymphocytic leukemia Hum. Reprod. 2000, 15:
762-766.
[20] Z. y. Wang, S. J. Chen, Z. C. Zhon, M. Athar, W. A. Khan, D .R.
Bickers, and H. Muthtar. Antimutagenic activity of green tea
polyphenols. Mut. Res., 1989, 223: 273-285.
[1] R. A. Mothana, and U. Lindequist. Antimicrobial activity of some
medicinal plants of the island Soqotra. J. Ethnopharmacol., 2005, 96(1-
2):177-181.
[2] K, Abeywickrama, and G. A. Bean. Toxigenic Aspergillus flavus and
aflatoxins in Sri Lankan medicinal plant material. Mycopa-thologia.,
1991, 113: 187-190.
[3] K. Gauthaman, A. P. Ganesan, and R. N. Prasad. "Sexual effects of
puncturevine (Tribulus terrestris) extract (protodioscin): an evaluation
using a rat model". Journal of Alternative and Complementary
Medicine., 2003, 9 (2): 257-265.
[4] S. H. Majeed, and M. J. Mahmood. Herbs and Medicinal Plants in Iraq
between Traditional Medicine and Scientific Research. 1st Ed. Baghdad:
Dar Al-Thaowra for Publishing., 1988, p. 40. (in Arabic).
[5] S. Aldein. Medicinal Herbs. 1st Ed. Baghdad: Dar Al-Shoun Al-
Thaqafia Al-Aama for Publishing.1986, p. 70. (in Arabic).
[6] M. M. Cowan. Plant products as antimicrobial agents. Clin.
Microbiol. Rev. 1999, 12(4): 564-582.
[7] O. Kandil, N. M. Radwan, A. B. Hassan, A. M. Amer, and H. A. El-
Banna. Extracts and fractions of Thymus capitatus exhibit antimicrobial
activities. J Ethnopharmacol. 1994, 44(1):19-24.
[8] D. McCan, L. Kirkis. Evaluation of Free Testosterone in serum. J Clin
Immunoassay. 1985, 8: 234-6.
[9] M. R. Atlas, E. Alfres, and C. Lawrence "Laboratory Manual
Experimental Microbiology" Mosby- Year Book, Inc. 1995.
[10] E. Evans, G. Breckon, and C. Ford.Air drying method for meiotic
preparation from mammalian testes. Cytogenetics 1964, 3: 284-294.
[11] E. Bedir, A. Khan. New steroidal glycosides from the fruits of Tribulus
terrestris. Pharmazie 2000, 57:491-3.
[12] Y. Wan, K. Ohtani, R. Kasai, and K. Yamasaki, Steroidal saponins from
fruits of Tribulus terrestris. Phytochemistry 1997, 45:811-7.
[13] A. G. Brown, M. D. Vukovich, E. R. Martini, L. M. Kohut, W. D. Frank,
DA Jackson, et al . Endocrine and lipid responses to chronic
androstenediol-herbal supplementation in 30 to 58 year old men. J Am
Coll Nutr 2002, 20:520-8.
[14] K. M. Arsyad. Effect of protodioscin on the quality and quantity of
sperms from males with moderate idiopathic oligozoospermia. Medica
1996, 22:614-8.
[15] S. K. Bhattacharya. National conference on Recent trends in Spice and
Medicinal Plants Research. Calcutta. India: April 1998, p. 652-4.
[16] B. Sun, W. Qu, and Z. Bai. The inhibitory effect of saponins from
Tribulus terrestris on Bcap-37 breast cancer line in vitro. Zhong Yao
Cai 2003, 26:104-6.
[17] Castoldi, G.; Scapoli, P.; and Spanedda, R. (1969). Chromosomes and
chloramphenicol. Arch. Ital. Patol. Clin. Tumori, 12:117-141.
[18] E.H. Al-Neimy, Biological effects of Capparis spinosa and Rumex
acetosella extracts on animal and human normal and tumor cells. Ph.D.
thesis, college of science, 2007, Al-Nahrain University.
[19] R. Chatterjee, G. A. Haines, D. M. Perera, A. Goldston, and L. D.
Morris. Testicular and sperm DNA damage after treatment with
fludarabin for chronic lymphocytic leukemia Hum. Reprod. 2000, 15:
762-766.
[20] Z. y. Wang, S. J. Chen, Z. C. Zhon, M. Athar, W. A. Khan, D .R.
Bickers, and H. Muthtar. Antimutagenic activity of green tea
polyphenols. Mut. Res., 1989, 223: 273-285.
@article{"International Journal of Chemical, Materials and Biomolecular Sciences:64490", author = "Ahmed A. Hussain and Abbas A. Mohammed and Heba. H. Ibrahim and Amir H. Abbas", title = "Study the Biological Activities of Tribulus Terrestris Extracts", abstract = "In this study the extracts of the Iraqi herb Tribulus
terrestris (Al-Hassage or Al-Kutub) was done by using of polar and
non polar solvents, then the biological activity of these extractants
was studied in three fields, First, the antibacterial activity (in vitro)
on gram positive bacteria (Staphylococcus aureus), and gram
negative bacteria (E. coli, Proteus vulgaris, Pseudomonas
aerugiuosa, and Klebsiella), all extracts showed considerable activity
against all bacteria. Second, the effect of extracts on free serum
testosterone level in male mice (in vivo), the alcoholic, and
acetonitrilic extracts showed significant (P < 0.05) increase in free
serum testosterone level, and we found that the extracts contained
compounds with less genotoxic effects in mice germ cells. 3rd, was to
study the effect of methanolic extract of T. terrestris in diabetes
management.", keywords = "Genotoxic, germ cells, tribulus terrestris, testosterone.", volume = "3", number = "9", pages = "547-3", }
