Abstract: Microbial-induced calcite precipitation (MICP) is a
relatively green and sustainable soil improvement technique. It
utilizes biochemical process that exists naturally in soil to improve
engineering properties of soils. The calcite precipitation process is
uplifted by the mean of injecting higher concentration of urease
positive bacteria and reagents into the soil. The main objective of this
paper is to provide an overview of the factors affecting the MICP in
soil. Several factors were identified including nutrients, bacteria type,
geometric compatibility of bacteria, bacteria cell concentration,
fixation and distribution of bacteria in soil, temperature, reagents
concentration, pH, and injection method. These factors were found to
be essential for promoting successful MICP soil treatment.
Furthermore, a preliminary laboratory test was carried out to
investigate the potential application of the technique in improving the
shear strength and impermeability of a residual soil specimen. The
results showed that both shear strength and impermeability of
residual soil improved significantly upon MICP treatment. The
improvement increased with increasing soil density.
Abstract: Silver-exchanged zeolites and clays are used in
polymer composites to confer broad-spectrum antimicrobial
properties on a range of functional materials. Tobermorite is a layer
lattice mineral whose potential as a carrier for Ag+ ions in
antibacterial composites has not yet been investigated. Accordingly,
in this study, synthetic tobermorite was ion-exchanged with 10 wt%
silver ions and the resulting material was incorporated into a
composite film with chitosan. Chitosan is a biocompatible,
biodegradable derivative of chitin, a polysaccharide obtained from
the shells of crustaceans. The solvent-cast Ag+-exchanged
tobermorite-chitosan films were found to exhibit antimicrobial action
against Staphylococcus aureus, Escherichia coli and Pseudomonas
aeruginosa.
Abstract: Adenylate kinase (AK) catalyse the phosphotransferase
reaction plays an important role in cellular energy homeostasis. The
inhibitors of bacterial AK are useful in the treatment of several
bacterial infections. To the novel inhibitors of AK, docking studies
performed by using the 3D structure of Bacillus stearothermophilus
adenylate kinase from protein data bank (IZIP). 46 Quinoxaline
analogues were docked in 1ZIP and selected the highly interacting
compounds based on their binding energies, for further studies
Abstract: The Zung self-depression scale and Beck Anxiety Inventory were used both to study the depression and anxiety levels of Armenian Crohn’s disease patients, and to reveal the relation between emotional status and placebo effect of these patients. On the other hand, the blood cell analyses and gut bacteria investigations were used to assess the placebo effect on ESR, and haemoglobin-s and leukocyte-s levels as well as gut commensal E. coli quantities of these patients. Despite of registered high levels of depression and anxiety, the high placebo effect on psychoemotional status for investigated patients during the investigations was described. On the other hand, no positive effect of placebo on measurements of ESP and hemoglobin-s levels of Crohn’s disease patients was revealed. The importance of use of psychotherapies for optimal outcomes during treatments of Crohn’s disease is discussed.
Abstract: This study was carried out to reveal the bacterial composition of aerosol in the studied abattoirs. Bacteria isolated were characterized according to microbiological standards. Factors such as temperature and distance were considered as variable in this study. The isolation was carried out at different temperatures such as 27oC, 31oC and 29oC and at various distances of 100meters and 200meters away from the slaughter sites. Result obtained showed that strains of Staphylococcus aureus, Escherichia coli, Bacillus subtilis, Lactobacillus alimentarius and Micrococcus sp. were identified. The total viable counts showed that more microorganisms were present in the morning while the least viable count of 388cfu was recorded in the evening period of this study. This study also showed that more microbial loads were recorded the further the distance is to the slaughter site. Conclusively, the array of bacteria isolated suggests that abattoir sites may be a potential source of pathogenic organisms to commuters if located within residential environment.
Abstract: Experiments were carried out at the Latvia State
Institute of Fruit-Growing in 2011. Fresh-cut minimally processed
apple and pear mixed salad were packed by passive modified
atmosphere (MAP) in PP containers, which were hermetically sealed
by breathable conventional BOPP PropafreshTM P2GAF, and Amcor
Agrifresh films. Biodegradable NatureFlexTM NVS INNOVIA Films
and VC999 BioPack PLA films coated with a barrier of pure silicon
oxide (SiOx) were used to compare the fresh-cut produce quality
with this packed in conventional packaging films. Samples were cold
stored at temperature +4.0±0.5 °C up to 10 days. The quality of salad
was evaluated by physicochemical properties – weight losses,
moisture, firmness, the effect of packaging modes on the colour,
dynamics in headspace atmosphere concentration (CO2 and O2),
titratable acidity values, as well as by microbiological contamination
(yeasts, moulds and total bacteria count) of salads, analyzing before
packaging and after 2, 4, 6, 8, and 10 storage days.
Abstract: Zirconium diamine and triamine complexes can possess biological activities. These complexes were synthesised via the reaction of equimolar quantities of 1,10-phenanthroline {NC3H3(C6H2)NC3H3} (L1) or 4-4-amino phenazone {ONC6H5(NH)CH(NH2} (L2) or diphenyl carbizon {HNNCO(NH)2(C6H5)} (L3) with a Zirconium Salt {ZrOCl2} in a 1:1 ratio to form complexes [{NC3H3(C6H2)NC3H3}ZrOCl2}] [ZrOCl2L1], [{(O2NC6H4(NH)(NH2)}ZrOCl2] [ZrOCl2L2] and [{HNNCO(NH)2(C6H5)ZrOCl2}] [ZrOCl2L3] respectively. They were characterised using Fourier Transform Infrared (FT-IR) and UV-Visible spectroscopy. Also a variable temperature study of these complexes was completed, using UV-Visible spectroscopy to observe electronic transitions under temperature control. Also a DFT study was done on these complexes via the information from FT-IR and UV-Visible spectroscopy.
These complexes were found to show different inhibition to the growth of bacterial strains of Bacillus spp. & Klebsiella spp. & E. coli & Proteus spp. & Pseudomona spp. at different concentrations (0.001, 0.2 and 1M). For better understanding these complexes were examined by using a Density Functional Theory (DFT) calculation.
Abstract: Human heart valves diseased by congenital heart
defects, rheumatic fever, bacterial infection, cancer may cause stenosis
or insufficiency in the valves. Treatment may be with medication but
often involves valve repair or replacement (insertion of an artificial
heart valve). Bileaflet mechanical heart valves (BMHVs) are widely
implanted to replace the diseased heart valves, but still suffer from
complications such as hemolysis, platelet activation, tissue
overgrowth and device failure. These complications are closely related
to both flow characteristics through the valves and leaflet dynamics. In
this study, the physiological flow interacting with the moving leaflets
in a bileaflet mechanical heart valve (BMHV) is simulated with a
strongly coupled implicit fluid-structure interaction (FSI) method
which is newly organized based on the Arbitrary-Lagrangian-Eulerian
(ALE) approach and the dynamic mesh method (remeshing) of
FLUENT. The simulated results are in good agreement with previous
experimental studies. This study shows the applicability of the present
FSI model to the complicated physics interacting between fluid flow
and moving boundary.
Abstract: Antifungal activities of ether and methanolic extracts of volatiles oils of Nigella Sativa seeds were tested against pathogenic bacterias and fungies strains.The volatile oil were found to have significant antifungal and antibacterial activities compare to tetracycline, cefuroxime and ciprofloxacin positive controls.The ether and methanolic esxtracts were compared to each other for antifungal and antibacterial activities and ether extracts showed stonger activity than methanolic one.
Abstract: The effect of plant growth-promoting rhizobacteria
(PGPR) on seed germination, seedling growth and yield of field
grown maize were evaluated in three experiments. In these
experiments six bacterial strains include P.putida strain R-168,
P.fluorescens strain R-93, P.fluorescens DSM 50090, P.putida
DSM291, A.lipoferum DSM 1691, A.brasilense DSM 1690 were
used. Results of first study showed seed Inoculation significantly
enhanced seed germination and seedling vigour of maize. In second
experiment, leaf and shoot dry weight and also leaf surface area
significantly were increased by bacterial inoculation in both sterile
and non-sterile soil. The results showed that inoculation with
bacterial treatments had a more stimulating effect on growth and
development of plants in nonsterile than sterile soil. In the third
experiment, Inoculation of maize seeds with all bacterial strains
significantly increased plant height, 100 seed weight, number of seed
per ear and leaf area .The results also showed significant increase in
ear and shoot dry weight of maize.
Abstract: Acute toxicity of nano SiO2, ZnO, MCM-41 (Meso
pore silica), Cu, Multi Wall Carbon Nano Tube (MWCNT), Single
Wall Carbon Nano Tube (SWCNT) , Fe (Coated) to bacteria Vibrio
fischeri using a homemade luminometer , was evaluated. The values
of the nominal effective concentrations (EC), causing 20% and 50%
inhibition of biouminescence, using two mathematical models at two
times of 5 and 30 minutes were calculated. Luminometer was
designed with Photomultiplier (PMT) detector. Luminol
chemiluminescence reaction was carried out for the calibration graph.
In the linear calibration range, the correlation coefficients and
coefficient of Variation (CV) were 0.988 and 3.21% respectively
which demonstrate the accuracy and reproducibility of the instrument
that are suitable. The important part of this research depends on how
to optimize the best condition for maximum bioluminescence. The
culture of Vibrio fischeri with optimal conditions in liquid media,
were stirring at 120 rpm at a temperature of 150C to 180C and were
incubated for 24 to 72 hours while solid medium was held at 180C
and for 48 hours. Suspension of nanoparticles ZnO, after 30 min
contact time to bacteria Vibrio fischeri, showed the highest toxicity
while SiO2 nanoparticles showed the lowest toxicity. After 5 min
exposure time, the toxicity of ZnO was the strongest and MCM-41
was the weakest toxicant component.
Abstract: The growing health hazardous impact of arsenic (As)
contamination in environment is the impetus of the present
investigation. Application of lactic acid bacteria (LAB) for the
removal of toxic and heavy metals from water has been reported.
This study was performed in order to isolate and characterize the Asresistant
LAB from mud and sludge samples for using as efficient As
uptaking probiotic. Isolation of As-resistant LAB colonies was
performed by spread plate technique using bromocresol purple
impregnated-MRS (BP-MRS) agar media provided with As @ 50
μg/ml. Isolated LAB were employed for probiotic characterization
process, acid and bile tolerance, lactic acid production, antibacterial
activity and antibiotic tolerance assays. After As-resistant and
removal characterizations, the LAB were identified using 16S rDNA
sequencing. A total of 103 isolates were identified as As-resistant
strains of LAB. The survival of 6 strains (As99-1, As100-2, As101-3,
As102-4, As105-7, and As112-9) was found after passing through the
sequential probiotic characterizations. Resistant pattern pronounced
hollow zones at As concentration >2000 μg/ml in As99-1, As100-2,
and As101-3 LAB strains, whereas it was found at ~1000 μg/ml in
rest 3 strains. Among 6 strains, the As uptake efficiency of As102-4
(0.006 μg/h/mg wet weight of cell) was higher (17 – 209%)
compared to remaining LAB. 16S rDNA sequencing data of 3 (As99-
1, As100-2, and As101-3) and 3 (As102-4, As105-7, and As112-9)
LAB strains clearly showed 97 to 99% (340 bp) homology to
Pediococcus dextrinicus and Pediococcus acidilactici, respectively.
Though, there was no correlation between the metal resistant and
removal efficiency of LAB examined but identified elevated As
removing LAB would probably be a potential As uptaking probiotic
agent. Since present experiment concerned with only As removal
from pure water, As removal and removal mechanism in natural
condition of intestinal milieu should be assessed in future studies.
Abstract: Nowadays, consumption of whole flours and flours
with high extraction rate is recommended, because of their high
amount of fibers, vitamins and minerals. Despite nutritional benefits
of whole flour, concentration of some undesirable components such
as phytic acid is higher than white flour. In this study, effect of
several lactic acid bacteria sourdough on Toast bread is investigated.
Sourdough from lactic acid bacteria (Lb. plantarum, Lb. reuteri) with
different dough yield (250 and 300) is made and incubated at 30°C
for 20 hour, then added to dough in the ratio of 10, 20 and 30%
replacement. Breads that supplemented with Lb. plantarum
sourdough had lower phytic acid. Higher replacement of sourdough
and higher DY cause higher decrease in phytic acid content.
Sourdough from Lb. plantarum, DY = 300 and 30% replacement
cause the highest decrease in phytic acid content (49.63 mg/100g).
As indicated by panelists, Lb. reuteri sourdough can present the
greatest effect on overall quality score of the breads. DY reduction
cause a decrease in bread quality score. Sensory score of Toast bread
is 81.71 in the samples that treated with Lb. reuteri sourdough with
DY = 250 and 20% replacement.
Abstract: A total of twenty tensile biopsies were collected from
children undergoing tonsillectomy from teaching hospital ENT
department and Kurdistan private hospital in sulaimani city. All
biopsies were homogenized and cultured; the obtained bacterial
isolates were purified and identified by biochemical tests and VITEK
2 compact system. Among the twenty studied samples, only one
Pseudomonas putida with probability of 99% was isolated.
Antimicrobial susceptibility was carried out by disk diffusion
method, Pseudomonas putida showed resistance to all antibiotics
used except vancomycin. The isolate further subjected to PCR and
DNA sequence analysis of blaVIM gene using different set of primers
for different regions of VIM gene. The results were found to be PCR
positive for the blaVIM gene. To determine the sequence of blaVIM
gene, DNA sequencing performed. Sequence alignment of blaVIM
gene with previously recorded blaVIM gene in NCBI- database showed
that P. putida isolate have different blaVIM gene.
Abstract: Field experiments were conducted at Annamalai University Experimental Farm, Department of Agronomy; to device suitable weed control measures for direct seeded puddled rice and to study the effect of the weed control measures on the soil microbial population. The treatments comprised of incorporation of pressmud @ 6.25 t ha-1 and application of herbicide butachlor @1.5 kg a. i. ha- 1 with and without safener 4 days after sowing (DAS), 8 DAS alone and also in conjunction with hand weeding at 30 DAS. Hand weeding twice and a weedy check were also maintained. At maximum tillering stage, the population of bacteria was significantly reduced by butachlor application. The injury to microbes caused by herbicide disappeared with the advancement of crop's age and at flowering stage of crop, there was no significant difference among the treatments. The fungal and actinomycetes population remained unaltered by weed control treatments at both the stages of observation.
Abstract: Surgical site infections (SSIs) are the most common
nosocomial infection in surgical patients resulting in significant
increases in postoperative morbidity and mortality. The commonly
causative bacteria developed resistance to virtually all antibiotics
available. The aim of this study was to isolation and identification the
most common bacteria that cause SSIs in Medical Research Institute,
and to compare their sensitivity to selected group of antibiotics and
natural products (garlic, oregano, olive, and Nigella sativa oils). The
isolated pathogens collected from infected surgical wounds were
identified, and their sensitivities to the antibiotics commonly
available for clinical use, and also to the different concentrations of
the used natural products were investigated. The results indicate to
the potential therapeutic effect of the tested natural products in
treatment of surgical wound infections.
Abstract: Polymerase chain reaction (PCR) assay and
conventional microbiological methods were used to detect bacterial
contamination of egg shells and egg content in different commercial
housing systems, open house system and evaporative cooling system.
A PCR assay was developed for direct detection using a set of
primers specific for the invasion by A gene (invA) of Salmonella spp.
PCR detected the presence of Salmonella in 2 samples of shell egg
from the evaporative cooling system, while conventional cultural
methods detected no Salmonella from the same samples.
Abstract: Novel polystrene-bound Schiff bases and their Pt(IV)
complexes have been prepared from condensation reaction of
polystyrene-A-NH2 with 2-hydroxybenzaldehyde and 5-fluoro-3-
bromo-2-hydroxybenzaldehyde. The structures of Pt(IV) complexes
with polystyrene including Schiff bases have been determined by
elemental analyses, magnetic susceptibility, IR, 1H-NMR, UV-vis,
TG/DTA and AAS. The antibacterial and antifungal activities of the
synthesized compounds have been studied by the well-diffusion
method against some selected microorganisms: (Bacillus cereus spp.,
Listeria monocytogenes 4b, Micrococcus luteus, Staphylococcus
aureus, Staphylococcus epidermis, Brucella abortus, Escherichia
coli, Pseudomonas putida spp., Shigella dysenteria type 10,
Salmonella typhi H).
Abstract: The effects of irrigation with dairy factory wastewater on soil properties were investigated at two sites that had received irrigation for > 60 years. Two adjoining paired sites that had never received DFE were also sampled as well as another seven fields from a wider area around the factory. In comparison with paired sites that had not received effluent, long-term wastewater irrigation resulted in an increase in pH, EC, extractable P, exchangeable Na and K and ESP. These changes were related to the use of phosphoric acid, NaOH and KOH as cleaning agents in the factory. Soil organic C content was unaffected by DFE irrigation but the size (microbial biomass C and N) and activity (basal respiration) of the soil microbial community were increased. These increases were attributed to regular inputs of soluble C (e.g. lactose) present as milk residues in the wastewater. Principal component analysis (PCA) of the soils data from all 11sites confirmed that the main effects of DFE irrigation were an increase in exchangeable Na, extractable P and microbial biomass C, an accumulation of soluble salts and a liming effect. PCA analysis of soil bacterial community structure, using PCR-DGGE of 16S rDNA fragments, generally separated individual sites from one another but did not group them according to irrigation history. Thus, whilst the size and activity of the soil microbial community were increased, the structure and diversity of the bacterial community remained unaffected.
Abstract: This study mainly aims at assessing the level of
microbial pollution of the water used in the chair system in dental
clinics. For this purpose 36 samples have been randomly collected
from a number of dental surgeries in the city of Tripoli in Libya.
However, 32 of the samples have tested positive to microbial
pollution including 13 of the samples, which have tested positives to
Pseudomonas aeruginosa. Based on the results of the test a further
investigation of the biofilms incorporated within the dental chair
system has been conducted. The laboratory tests of biofilms with
similar design to those found in dental chairs have proved that
bacterial pollution takes place through saliva of the patients who use
the chairs, and that this saliva is rich with nutrients which provides a
suitable breeding ground for all types of bacteria.