Abstract: The growing health hazardous impact of arsenic (As)
contamination in environment is the impetus of the present
investigation. Application of lactic acid bacteria (LAB) for the
removal of toxic and heavy metals from water has been reported.
This study was performed in order to isolate and characterize the Asresistant
LAB from mud and sludge samples for using as efficient As
uptaking probiotic. Isolation of As-resistant LAB colonies was
performed by spread plate technique using bromocresol purple
impregnated-MRS (BP-MRS) agar media provided with As @ 50
μg/ml. Isolated LAB were employed for probiotic characterization
process, acid and bile tolerance, lactic acid production, antibacterial
activity and antibiotic tolerance assays. After As-resistant and
removal characterizations, the LAB were identified using 16S rDNA
sequencing. A total of 103 isolates were identified as As-resistant
strains of LAB. The survival of 6 strains (As99-1, As100-2, As101-3,
As102-4, As105-7, and As112-9) was found after passing through the
sequential probiotic characterizations. Resistant pattern pronounced
hollow zones at As concentration >2000 μg/ml in As99-1, As100-2,
and As101-3 LAB strains, whereas it was found at ~1000 μg/ml in
rest 3 strains. Among 6 strains, the As uptake efficiency of As102-4
(0.006 μg/h/mg wet weight of cell) was higher (17 – 209%)
compared to remaining LAB. 16S rDNA sequencing data of 3 (As99-
1, As100-2, and As101-3) and 3 (As102-4, As105-7, and As112-9)
LAB strains clearly showed 97 to 99% (340 bp) homology to
Pediococcus dextrinicus and Pediococcus acidilactici, respectively.
Though, there was no correlation between the metal resistant and
removal efficiency of LAB examined but identified elevated As
removing LAB would probably be a potential As uptaking probiotic
agent. Since present experiment concerned with only As removal
from pure water, As removal and removal mechanism in natural
condition of intestinal milieu should be assessed in future studies.
Abstract: The present study attempted to improve the Mercury
(Hg) sorption capacity of kanuma volcanic ash soil (KVAS) by
impregnating the cupper (Cu). Impregnation was executed by 1 and
5% Cu powder and sorption characterization of optimum Hg
removing Cu impregnated KVAS was performed under different
operational conditions, contact time, solution pH, sorbent dosage and
Hg concentration using the batch operation studies. The 1% Cu
impregnated KVAS pronounced optimum improvement (79%) in
removing Hg from water compare to control. The present
investigation determined the equilibrium state of maximum Hg
adsorption at 6 h contact period. The adsorption revealed a pH
dependent response and pH 3.5 showed maximum sorption capacity
of Hg. Freundlich isotherm model is well fitted with the experimental
data than that of Langmuir isotherm. It can be concluded that the Cu
impregnation improves the Hg sorption capacity of KVAS and 1%
Cu impregnated KVAS could be employed as cost-effective
adsorbent media for treating Hg contaminated water.