Manipulation of Probiotics Fermentation of Yogurt by Cinnamon and Licorice: Effects on Yogurt Formation and Inhibition of Helicobacter Pylori Growth in vitro
Probiotic bacteria especially Lactobacillus spp. and Bifidobacterium exert suppressive effect on Helicobacter pylori. Cinnamon and licorice have been traditionally used for the treatment of gastric ulcer. The objectives of this study were to determine the effects of herbs on yogurt fermentation, the level of probiotic bacteria in yogurt during 28 days storage and the effect of herbal yogurt on the growth of H. pylori in vitro. Cinnamon or licorice was mixed with milk and the mixture was fermented with probiotic bacteria to form herbal-yogurt. Changes of pH and total titratable acids were monitored and the viability of probiotic bacteria was evaluated during and after refrigerated storage. The in vitro inhibition of H. pylori growth was determined using agar diffusion and minimum inhibitory concentration (MIC) method. The presence of herbs did not affect the probiotic population during storage. There were no significant differences in pH and TTA between herbal-yogurts and plain-yogurt during fermentation and storage. Water extract of cinnamon-yogurt showed the highest inhibition effect (13.5mm) on H. pylori growth in comparison with licorice-yogurt (11.2mm). The present findings indicate cinnamon and licorice has bioactive components to decrease the growth of H. pylori.
[1] Stiles, M. E., & Holzapfel, W. H. Lactic acid bacteria of foods and their
current taxonomy. International Journal of Food Microbiology, 1997,
36: 1-29.
[2] Metchnikoff, I. I. The prolongation of life: Optimistic studies (reprinted
edition 1907). New York, NY, USA: Springer 2004.
[3] Coconnier MH, Lievin V, Hemery E. Antagonistic activity against
Helicobacter infection in vitro and in vivo by the human Lactobacillus
acidophilus strain LB. Appl Environ Microbiol, 1998, 64: 4573-80
[4] Midolo PD, Lambert JR, Hull R. In vitro inhibition of Helicobacter
pylori NCTC 11637 by organic acids and lactic acid bacteria. J Appl
Bacteriol, 1995, 79: 475-9
[5] Sheu BS, Wu JJ, Lo CY. Lactobacillus and Bifidobacteria containing
yogurt can improve the drug compliance of triple therapy for
Helicobacter pylori eradication. Aliment Pharmacol Ther, 2002, 16:
1669-75
[6] Wang KY, Li SN, Liu C. Effects of ingesting Lactobacillus- and
Bifidobacterium-containing yogurt in subjects with colonized
Helicobacter pylori. Am J Clin Nutr, 2004, 80: 737-41
[7] Borsch G. Campylobacter pylori: new and renewed insights into
gastritis-associated ulcer disease (GAUD). Hepato-Gastroenterology,
1987, 34: 191-3.
[8] Tytgat GN, Rauws EA. The role of Campylobacter pylori in
gastroduodenal diseases. A ÔÇÿbeliever-s- point of view. Gastroenterol Clin
Bio, 1989, 13:118B-21B.
[9] EUROGAST study group: An International Association between
Helicobacter pylori infection and Gastric Cancer. Lancet 1993, 341:
1359-1362.
[10] Marshall BJ, Goodwin CS, Warren JR, Murray R, Blincow ED,
Blackbourn SJ, Phillips M, Waters TE, and Sanderson CR: Prospective
double blind trail of duodenal ulcer relapse after eradication of
Campylobacter pylori. Lancet 1988, 2: 1437-1442.
[11] Rauws EA, Langenberg W, Houthoff HJ, Zanen HC, Tytgat GN:
Campylobacter pyloridis -associated chronic active antral gastritis. A
prospective study of its prevalence and the effects of antibacterial and
antiulcer treatment. Gastroenterol 1988, 94: 33-40.
[12] Lacy BE, Rosemore J: Helicobacter pylori: ulcers and more: the
beginning of an era. J Nutr 2001, 131: 2789S-2793S
[13] Buiatti E, Munoz N, Vivas J, Cano E, Peraza S: Difficulty in eradicating
Helicobacter pylori in a population at high risk for gastric cancer in
Venezuela. Cancer Causes Control 1994, 5: 249-254.
[14] Bell GD, Powell KU, Burridge SM, Bowden AN, Ramesh B, Bolten G,
Purser K, Harrison G, Brown C, Gant PW, Jones PH, Trowell JE:
Helicobacter pylori eradication: efficacy and side effect profile of a
combination of omeprazole, amoxycillin and metronidazole compared
with four alternative regimens. QJ Med J Assoc Phys 1993, 86: 743-750.
[15] Broutet N, Tchamgoue S, Pereira E, Lamouliatte H, Salamon R,
Megraud F: Risk factors for failure of Helicobacter pylori therapyresults
of an individual data analysis of 2751 patients. Aliment Pharmacol Ther
2003, 17: 99-109.
[16] O'Gara EA, Hill DJ, Maslin DJ: Activities of garlic oil, garlic powder,
and their diallyl constituents against Helicobacter pylori. Appl Environ
Microbiol 2000, 66: 2269-2273.
[17] Tabak M, Armon R, Potasman I, Neeman I. In vitro inhibition of
Helicobacter pylori by extracts of Thyme. J Appl Bacteriol 1996, 80:
667-72.
[18] Sheu B-S, Wu J-J, Lo C-Y, et al. Impact of supplement with
Lactobacillus - and Bifidobacterium-containing yogurt on triple therapy
for Helicobacter pylori eradication. Aliment Pharmacol Ther 2002, 16:
1669-75.
[19] Hood, S. K., & Zottola, M. L. Effects of low PH on the ability of
Lactobacillus acidophilus to survive and adhere to human intestinal
cells. J Food Science 1998, 53: 1514-1516.
[20] Shah, N. P. Probiotic bacteria, Selective enumeration and survival in
dairy foods. Journal of Dairy Science 2000, 83: 894-907.
[21] Zaika, L.L. (1988). Spices and herbs: their antimicrobial activity and its
determination. J Food Safety 1988, 9: 97-118.
[22] Shahamat, M., Mai, U.E.H., Paszko-Kolva, C., Yamamoto, H., Colwell,
R.R. Evaluation of liquid media for growth of Helicobacter pylori. J.
Clin. Microbiol 1991, 29: 2835-2837.
[1] Stiles, M. E., & Holzapfel, W. H. Lactic acid bacteria of foods and their
current taxonomy. International Journal of Food Microbiology, 1997,
36: 1-29.
[2] Metchnikoff, I. I. The prolongation of life: Optimistic studies (reprinted
edition 1907). New York, NY, USA: Springer 2004.
[3] Coconnier MH, Lievin V, Hemery E. Antagonistic activity against
Helicobacter infection in vitro and in vivo by the human Lactobacillus
acidophilus strain LB. Appl Environ Microbiol, 1998, 64: 4573-80
[4] Midolo PD, Lambert JR, Hull R. In vitro inhibition of Helicobacter
pylori NCTC 11637 by organic acids and lactic acid bacteria. J Appl
Bacteriol, 1995, 79: 475-9
[5] Sheu BS, Wu JJ, Lo CY. Lactobacillus and Bifidobacteria containing
yogurt can improve the drug compliance of triple therapy for
Helicobacter pylori eradication. Aliment Pharmacol Ther, 2002, 16:
1669-75
[6] Wang KY, Li SN, Liu C. Effects of ingesting Lactobacillus- and
Bifidobacterium-containing yogurt in subjects with colonized
Helicobacter pylori. Am J Clin Nutr, 2004, 80: 737-41
[7] Borsch G. Campylobacter pylori: new and renewed insights into
gastritis-associated ulcer disease (GAUD). Hepato-Gastroenterology,
1987, 34: 191-3.
[8] Tytgat GN, Rauws EA. The role of Campylobacter pylori in
gastroduodenal diseases. A ÔÇÿbeliever-s- point of view. Gastroenterol Clin
Bio, 1989, 13:118B-21B.
[9] EUROGAST study group: An International Association between
Helicobacter pylori infection and Gastric Cancer. Lancet 1993, 341:
1359-1362.
[10] Marshall BJ, Goodwin CS, Warren JR, Murray R, Blincow ED,
Blackbourn SJ, Phillips M, Waters TE, and Sanderson CR: Prospective
double blind trail of duodenal ulcer relapse after eradication of
Campylobacter pylori. Lancet 1988, 2: 1437-1442.
[11] Rauws EA, Langenberg W, Houthoff HJ, Zanen HC, Tytgat GN:
Campylobacter pyloridis -associated chronic active antral gastritis. A
prospective study of its prevalence and the effects of antibacterial and
antiulcer treatment. Gastroenterol 1988, 94: 33-40.
[12] Lacy BE, Rosemore J: Helicobacter pylori: ulcers and more: the
beginning of an era. J Nutr 2001, 131: 2789S-2793S
[13] Buiatti E, Munoz N, Vivas J, Cano E, Peraza S: Difficulty in eradicating
Helicobacter pylori in a population at high risk for gastric cancer in
Venezuela. Cancer Causes Control 1994, 5: 249-254.
[14] Bell GD, Powell KU, Burridge SM, Bowden AN, Ramesh B, Bolten G,
Purser K, Harrison G, Brown C, Gant PW, Jones PH, Trowell JE:
Helicobacter pylori eradication: efficacy and side effect profile of a
combination of omeprazole, amoxycillin and metronidazole compared
with four alternative regimens. QJ Med J Assoc Phys 1993, 86: 743-750.
[15] Broutet N, Tchamgoue S, Pereira E, Lamouliatte H, Salamon R,
Megraud F: Risk factors for failure of Helicobacter pylori therapyresults
of an individual data analysis of 2751 patients. Aliment Pharmacol Ther
2003, 17: 99-109.
[16] O'Gara EA, Hill DJ, Maslin DJ: Activities of garlic oil, garlic powder,
and their diallyl constituents against Helicobacter pylori. Appl Environ
Microbiol 2000, 66: 2269-2273.
[17] Tabak M, Armon R, Potasman I, Neeman I. In vitro inhibition of
Helicobacter pylori by extracts of Thyme. J Appl Bacteriol 1996, 80:
667-72.
[18] Sheu B-S, Wu J-J, Lo C-Y, et al. Impact of supplement with
Lactobacillus - and Bifidobacterium-containing yogurt on triple therapy
for Helicobacter pylori eradication. Aliment Pharmacol Ther 2002, 16:
1669-75.
[19] Hood, S. K., & Zottola, M. L. Effects of low PH on the ability of
Lactobacillus acidophilus to survive and adhere to human intestinal
cells. J Food Science 1998, 53: 1514-1516.
[20] Shah, N. P. Probiotic bacteria, Selective enumeration and survival in
dairy foods. Journal of Dairy Science 2000, 83: 894-907.
[21] Zaika, L.L. (1988). Spices and herbs: their antimicrobial activity and its
determination. J Food Safety 1988, 9: 97-118.
[22] Shahamat, M., Mai, U.E.H., Paszko-Kolva, C., Yamamoto, H., Colwell,
R.R. Evaluation of liquid media for growth of Helicobacter pylori. J.
Clin. Microbiol 1991, 29: 2835-2837.
@article{"International Journal of Biological, Life and Agricultural Sciences:64772", author = "S. Behrad and M.Y. Yusof and K. L. Goh and A.S. Baba", title = "Manipulation of Probiotics Fermentation of Yogurt by Cinnamon and Licorice: Effects on Yogurt Formation and Inhibition of Helicobacter Pylori Growth in vitro", abstract = "Probiotic bacteria especially Lactobacillus spp. and Bifidobacterium exert suppressive effect on Helicobacter pylori. Cinnamon and licorice have been traditionally used for the treatment of gastric ulcer. The objectives of this study were to determine the effects of herbs on yogurt fermentation, the level of probiotic bacteria in yogurt during 28 days storage and the effect of herbal yogurt on the growth of H. pylori in vitro. Cinnamon or licorice was mixed with milk and the mixture was fermented with probiotic bacteria to form herbal-yogurt. Changes of pH and total titratable acids were monitored and the viability of probiotic bacteria was evaluated during and after refrigerated storage. The in vitro inhibition of H. pylori growth was determined using agar diffusion and minimum inhibitory concentration (MIC) method. The presence of herbs did not affect the probiotic population during storage. There were no significant differences in pH and TTA between herbal-yogurts and plain-yogurt during fermentation and storage. Water extract of cinnamon-yogurt showed the highest inhibition effect (13.5mm) on H. pylori growth in comparison with licorice-yogurt (11.2mm). The present findings indicate cinnamon and licorice has bioactive components to decrease the growth of H. pylori.
", keywords = "Cinnamon, Helicobacter pylori, Herbal-Yogurt,Licorice, Probiotics", volume = "3", number = "12", pages = "586-5", }
