Abstract: Safe drinking water is one of the biggest issues facing
the planet this century. The primary aim of this paper is to present our
research focused on theoretical and experimental analysis of potable
water and in-building water distribution systems from the point of
view of microbiological risk on the basis of confrontation between
the theoretical analysis and synthesis of gathered information in
conditions of the Slovak Republic. The presence of the bacteria
Legionella in water systems, especially in hot water distribution
system, represents in terms of health protection of inhabitants the
crucial problem which cannot be overlooked. Legionella
pneumophila discovery, its classification and its influence on
installations inside buildings are relatively new. There are a lot of
guidelines and regulations developed in many individual countries for
the design, operation and maintenance for tap water systems to avoid
the growth of bacteria Legionella pneumophila, but in Slovakia we
don-t have any. The goal of this paper is to show the necessity of
prevention and regulations for installations inside buildings verified
by simulation methods.
Abstract: Legionella pneumophila is involved in more than 95%
cases of severe atypical pneumonia. Infection is mainly by
inhalation the indoor aerosols through the water-coolant systems.
Because some Legionella strains may be viable but not culturable,
therefore, Taq polymerase, DNA amplification and semi-nested-PCR
were carried out to detect Legionella-specific 16S-rDNA sequence.
For this purpose, 1.5 litter of water samples from 77 water-coolant
system were collected from four different hospitals, two nursing
homes and one student hostel in Kerman city of Iran, each in a brand
new plastic bottle during summer season of 2006 (from April to
August). The samples were filtered in the sterile condition through
the Millipore Membrane Filter. DNA was extracted from membrane
and used for PCR to detect Legionella spp. The PCR product was
then subjected to semi-nested PCR for detection of L. pneumophila.
Out of 77 water samples that were tested by PCR, 30 (39%) were
positive for most species of Legionella. However, L. pneumophila
was detected from 14 (18.2%) water samples by semi-nested PCR.
From the above results it can be concluded that water coolant
systems of different hospitals and nursing homes in Kerman city of
Iran are highly contaminated with L. pneumophila spp. and pose
serious concern. So, we recommend avoiding such type of coolant
system in the hospitals and nursing homes.