Abstract: 10 clinically healthy hemal nodes were collected from male bulls aged 2-3 years. Light microscopy revealed a capsule of connective tissue consisted mainly of collagen fiber surrounding hemal node, numerous erythrocytes were found in wide subcapsular sinus under the capsule. The parenchyma of the hemal node was divided into cortex and medulla. Diffused lymphocytes, and lymphoid follicles, having germinal centers were the main components of the cortex, while in the medulla there was wide medullary sinus, diffused lymphocytes and few lymphoid nodules. The area occupied with lymph nodules was larger than that occupied with non-nodular structure of lymphoid cords and blood sinusoids. Electron microscopy revealed the cellular components of hemal node including elements of circulating erythrocytes intermingled with lymphocytes, plasma cells, mast cells, reticular cells, macrophages, megakaryocytes and endothelial cells lining the blood sinuses. The lymphocytes were somewhat triangular in shape with cytoplasmic processes extending between adjacent erythrocytes. Nuclei were triangular to oval in shape, lightly stained with clear nuclear membrane indentation and clear nucleoli. The reticular cells were elongated in shape with cytoplasmic processes extending between adjacent lymphocytes, rough endoplasmic reticulum, ribosomes and few lysosomes were seen in their cytoplasm. Nucleus was elongated in shape with less condensed chromatin. Plasma cells were oval to irregular in shape with numerous dilated rough endoplasmic reticulum containing electron lucent material occupying the whole cytoplasm and few mitochondria were found. Nuclei were centrally located and oval in shape with heterochromatin emarginated and often clumped near the nuclear membrane. Occasionally megakaryocytes and mast cells were seen among lymphocytes. Megakaryocytes had multilobulated nucleus and free ribosomes often appearing as small aggregates in their cytoplasm, while mast cell had their characteristic electron dense granule in the cytoplasm, few electron lucent granules were found also, we conclude that, the main function of the hemal node of cattle is proliferation of lymphocytes. No role for plasma cell in erythrophagocytosis could be suggested.
Abstract: Surface characteristics of Bacillus megaterium strain
were investigated; zeta potential, FTIR and contact angle were
measured. Surface energy components including Lifshitz-van der
Waals, Hamaker constant, and acid/base components (Lewis
acid/Lewis base) were calculated from the contact angle data. The
results showed that the microbial cells were negatively charged over
all pH regions with high values at alkaline region. A hydrophilic
nature for the strain was confirmed by contact angle and free energy
of adhesion between microbial cells. Adsorption affinity of the strain
toward dolomite was studied at different pH values. The results
showed that the cells had a high affinity to dolomite at acid pH
comparing to neutral and alkaline pH. Extended DLVO theory was
applied to calculate interaction energy between B. megaterium cells
and dolomite particles. The adsorption results were in agreement with
the results of Extended DLVO approach. Surface changes occurred
on dolomite surface after the bio-treatment were monitored; contact
angle decreased from 69° to 38° and the mineral’s floatability
decreased from 95% to 25% after the treatment.
Abstract: Present paper enumerates highlights of seasonal
variation in floristic composition and ecological strategies for the
management of ‘Gujar Tal’ at Jaunpur in tropical semi-arid region of
eastern U.P. (India). Total composition of macrophytes recorded was
47 from 26 families with maximum 6 plant species of Cyperaceae
from April, 2012 to March, 2013 at certain periodic intervals.
Maximum number of plants (39) was present during winter followed
by (37) rainy and (27) summer seasons. The distribution pattern
depicted that maximum number of plants (27) was of marshy and
swampy habitats usually transitional between land and water.
Abstract: In this study, total fatty acid composition of muscle
lipids of Cyprinus carpio L. living in Suğla Dam Lake, Altinapa Dam
Lake, Eğirdir Lake and Burdur Lake were determined using GC.
During this study, for the summer season of July was taken from each
region of the land and they were stored in deep-freeze set to -20
degrees until the analysis date. At the end of the analyses, 30
different fatty acids were found in the composition of Cyprinus
carpio L. which lives in different lakes. Cyprinus carpio Suğla Dam
Lake of polyunsaturated fatty acids (PUFAs), were higher than other
lakes. Cyprinus carpio L. was the highest in the major SFA palmitic
acid. Polyunsaturated fatty acids (PUFA) of carp, the most abundant
fish species in all lakes, were found to be higher than those of
saturated fatty acids (SFA) in all lakes. Palmitic acid was the major
SFA in all lakes. Oleic acid was identified as the major MUFA.
Docosahexaenoic acid (DHA) was the most abundant in all lakes. ω3
fatty acid composition was higher than the percentage of the
percentage ω6 fatty acids in all lake. ω3/ω6 rates of Cyprinus carpio
L. Suğla Dam Lake, Altinapa Dam Lake, Eğirdir Lake and Burdur
Lake, 2.12, 1.19, 2.15, 2.87, and 2.82, respectively. Docosahexaenoic
acid (DHA) was the major PUFA in Eğirdir and Burdur lakes,
whereas linoleic acid (LA) was the major PUFA in Altinapa and
Suğla Dam Lakes. It was shown that the fatty acid composition in the
muscle of carp was significantly influenced by different lakes.
Abstract: The occurrence of adult Taenia saginata in major
abattoirs in Port Harcourt metropolis was investigated. Out of 514
cattle investigated, an overall prevalence of 35(6.8%) was recorded.
Infected male and female cattle represented 1.2% (6/514) and 5.6%
(29/514) of the overall prevalence respectively. There was a
statistical significant difference (P< 0.05) in prevalence of adult
Taenaia saginata between male and female cattle examined in the
study area. Old cattle have a significant (P< 0.05) infestation rate
than young ones. Adult Taenia saginata exists in cattle and still
remains a public health concern in the study area. Deliberate effort is
needed from stake-holders and the Government to design and
implement programs that will lead to the prevention and possible
eradication of the parasite.
Abstract: Medicinal and aromatic plants are promising and are characterized by the biosynthesis of odorous molecules that make up the so-called essential oils (EO), which have long been known for their antiseptic and therapeutic activity in folk medicine. Essential oils have many therapeutic properties. In herbal medicine, they are used for their antiseptic properties against infectious diseases of fungal origin, against dermatophytes, those of bacterial origin. The objective of this study was to evaluate the influence of drying in the shade on the water content and on the content of essential oils extracted from leaves of Eucalyptus camendulensis for better quality control of medicinal and aromatic plants. The water content of the Eucalyptus camendulensis plant material decreases during the drying process. It decreased from 100% to 0.006% for the drying in the shade after ten days. The moisture content is practically constant at the end of the drying period. The drying in the shade increases the concentration of essential oils of Eucalyptus camendulensis. When the leaves of Eucalyptus camendulensis plant are in the shade, the maximum of the essential oil content was obtained on the eighth day, the recorded value was 1.43% ± 0.01%. Beyond these periods, the content continuously drop in before stabilizing. The optimum drying time is between 6 and 9 days.
Abstract: Landfill waste is a common problem as it has an
economic and environmental impact even if it is closed. Landfill
waste contains a high density of various persistent compounds such
as heavy metals, organic and inorganic materials. As persistent
compounds are slowly-degradable or even non-degradable in the
environment, they often produce sublethal or even lethal effects on
aquatic organisms. The aims of the present study were to estimate
sublethal effects of the Kairiai landfill (WGS: 55°55‘46.74“,
23°23‘28.4“) leachate on the locomotor activity of rainbow trout
Oncorhynchus mykiss juveniles using the original system package
developed in our laboratory for automated monitoring, recording and
analysis of aquatic organisms’ activity, and to determine patterns of
fish behavioral response to sublethal effects of leachate. Four
different concentrations of leachate were chosen: 0.125; 0.25; 0.5 and
1.0 mL/L (0.0025; 0.005; 0.01 and 0.002 as part of 96-hour LC50,
respectively). Locomotor activity was measured after 5, 10 and 30
minutes of exposure during 1-minute test-periods of each fish (7 fish
per treatment). The threshold-effect-concentration amounted to 0.18
mL/L (0.0036 parts of 96-hour LC50). This concentration was found
to be even 2.8-fold lower than the concentration generally assumed to
be “safe” for fish. At higher concentrations, the landfill leachate
solution elicited behavioral response of test fish to sublethal levels of
pollutants. The ability of the rainbow trout to detect and avoid
contaminants occurred after 5 minutes of exposure. The intensity of
locomotor activity reached a peak within 10 minutes, evidently
decreasing after 30 minutes. This could be explained by the
physiological and biochemical adaptation of fish to altered
environmental conditions. It has been established that the locomotor
activity of juvenile trout depends on leachate concentration and
exposure duration. Modeling of these parameters showed that the
activity of juveniles increased at higher leachate concentrations, but
slightly decreased with the increasing exposure duration. Experiment
results confirm that the behavior of rainbow trout juveniles is a
sensitive and rapid biomarker that can be used in combination with
the system for fish behavior monitoring, registration and analysis to
determine sublethal concentrations of pollutants in ambient water.
Further research should be focused on software improvement aimed
to include more parameters of aquatic organisms’ behavior and to
investigate the most rapid and appropriate behavioral responses in
different species. In practice, this study could be the basis for the
development and creation of biological early-warning systems
(BEWS).
Abstract: The beginning of 21st century has witnessed new
advancements in the design and use of new materials for biosensing
applications, from nano to macro, protein to tissue. Traditional
analytical methods lack a complete toolset to describe the
complexities introduced by living systems, pathological relations,
discrete hierarchical materials, cross-phase interactions, and
structure-property dependencies. Materiomics – via systematic
molecular dynamics (MD) simulation – can provide structureprocess-
property relations by using a materials science approach
linking mechanisms across scales and enables oriented biosensor
design. With this approach, DNA biosensors can be utilized to detect
disease biomarkers present in individuals’ breath such as acetone for
diabetes. Our wireless sensor array based on single-stranded DNA
(ssDNA)-decorated single-walled carbon nanotubes (SWNT) has
successfully detected trace amount of various chemicals in vapor
differentiated by pattern recognition. Here, we present how MD
simulation can revolutionize the way of design and screening of DNA
aptamers for targeting biomarkers related to oral diseases and oral
health monitoring. It demonstrates great potential to be utilized to
build a library of DNDA sequences for reliable detection of several
biomarkers of one specific disease, and as well provides a new
methodology of creating, designing, and applying of biosensors.
Abstract: In order to study the Mutual effect of genotype ×
environment for the percent of oil index in sunflower items, an
experiment was accomplished form complete random block designs
in four iteration and was four diverse researching station comprising
Esfahan, Birjand, Sari, and Karaj. Complex variance analysis showed
that there is an important diversity between the items under
investigation. The results relevant the coefficient variation of items
Azargol and Vidoc has respectively allocated the minimum
coefficient of variations. According to the results extrapolated from
Shokla stability variance, the Items Brocar, Allison and Fabiola, are
among the stable genotypes for oil percent respectively. In the biplot
GGE, the location under investigations divided in two superenvironments,
first one comprised of locations naming Esfahan,
Karaj, and Birjand, and second one were such a location as Sari. By
this point of view, in the first super-environment, the Item Fabiola
and in the second Almanzor item was among the best items and
crops.
Abstract: The research aims to investigate the occurrence of
multidrug-resistant Acinetobacter, in carrot and estimate the role of
carrot in its transmission in a rapidly growing urban population.
Thus, 50 carrot samples were collected from Jakara wastewater
irrigation farms and are analyzed on MacConkey agar and screened
by Microbact 24E (Oxoid) and susceptibility of isolates is tested
against 10 commonly used antibiotics. Acinetobacter baumannii and
A. lwoffii were isolated in 22.00% and 16% of samples respectively.
Resistance to ceporex and penicillin of 36.36% and 27.27% in A.
baumannii, and sensitivity to ofloxacin, pefloxacin, gentimycin and
co-trimoxazole were observed. However, for A. lwoffii apart from
37.50% resistance to ceporex, it was also resistant to all other drugs
tested. There were similarities in the resistances shown by A.
baumannii and A. lwoffii to fluoroquinolones and β- lactame drug
families in addition to between sulfonamide and animoglycoside
demonstrated by A. lwoffii. Significant correlation in similarities were
observed at P < 0.05 to CPX to NA (46.2%), and SXT to AU (52.6%)
A. baumannii and A. lwoffii respectively and high multi drug
resistance (MDR) of 27.27% and 62.50% by A. baumannii and A.
lwoffii respectively. The occurrence of multidrug-resistance pathogen
in carrot is a serious challenge to public health care, especially in a
rapidly growing urban population where subsistence agriculture
contributes greatly to urban livelihood and source of vegetables.
Abstract: Fermentation is well known as an essential process to
develop chocolate flavor in dried cocoa beans. Besides developing
the precursor of cocoa flavor, it also induces the color changes in the
beans. The fermentation process is influenced by various factors such
as planting material, preconditioning of cocoa pod and fermentation
technique. Therefore, this study was conducted to evaluate color of
Malaysian cocoa beans and how the duration of pods storage and
fermentation technique using shallow box will effect on its color
characteristics. There are two factors being studied i.e. duration of
cocoa pod storage (0, 2, 4 and 6 days) and duration of cocoa
fermentation (0, 1, 2, 3, 4 and 5 days). The experiment is arranged in
4 x 6 factorial designs with 24 treatments and arrangement is in a
Completely Randomised Design (CRD). The produced beans are
inspected for color changes under artificial light during cut test and
divided into four groups of color namely fully brown, purple brown,
fully purple and slaty. Cut tests indicated that cocoa beans which are
directly dried without undergone fermentation has the highest slaty
percentage. However, application of pods storage before fermentation
process is found to decrease the slaty percentage. In contrast, the
percentages of fully brown beans start to dominate after two days of
fermentation, especially from four and six days of pods storage batch.
Whereas, almost all batches of cocoa beans have a percentage of fully
purple less than 20%. Interestingly, the percentage of purple brown
beans are scattered in the entire beans batch regardless any specific
trend. Meanwhile, statistical analysis using General Linear Model
showed that the pods storage has a significant effect on the color
characteristic of the Malaysian dried beans compared to fermentation
duration.
Abstract: Dietary cholesterol has elicited the most public
interest as it relates with coronary heart disease. Thus, humans have
been paying more attention to health, thereby reducing consumption
of cholesterol enriched food. Egg is considered as one of the major
sources of human dietary cholesterol. However, an alternative way to
reduce the potential cholesterolemic effect of eggs is to modify the
fatty acid composition of the yolk. The effect of palm oil (PO),
soybean oil (SO), sesame seed oil (SSO) and fish oil (FO)
supplementation in the diets of layers on egg yolk fatty acid,
cholesterol, egg production and egg quality parameters were
evaluated in a 42-day feeding trial. One hundred and five Isa Brown
laying hens of 34 weeks of age were randomly distributed into seven
groups of five replicates and three birds per replicate in a completely
randomized design. Seven corn-soybean basal diets (BD) were
formulated: BD+No oil (T1), BD+1.5% PO (T2), BD+1.5% SO (T3),
BD+1.5% SSO (T4), BD+1.5% FO (T5), BD+0.75% SO+0.75% FO
(T6) and BD+0.75% SSO+0.75% FO (T7). Five eggs were randomly
sampled at day 42 from each replicate to assay for the cholesterol,
fatty acid profile of egg yolk and egg quality assessment. Results
showed that there were no significant (P>0.05) differences observed
in production performance, egg cholesterol and egg quality
parameters except for yolk height, albumen height, yolk index, egg
shape index, haugh unit, and yolk colour. There were no significant
differences (P>0.05) observed in total cholesterol, high density
lipoprotein and low density lipoprotein levels of egg yolk across the
treatments. However, diets had effect (P
Abstract: Objective: Safety and efficacy of Ahmed glaucoma
valve implantation for the management of uveitis induced glaucoma
evaluated on the five dogs with uncontrollable glaucoma. Materials
and Methods: Ahmed Glaucoma Valve (AGV®; New World
Medical, Rancho Cucamonga, CA, USA) is a flow restrictive, nonobstructive
self-regulating valve system. Preoperative ocular
evaluation included direct ophthalmoscopy and measurement of the
intraocular pressure (IOP). The implant was examined and primed
prior to implantation. The selected site of the valve implantation was
the superior quadrant between the superior and lateral rectus muscles.
A fornix-based incision was made through the conjunectiva and
Tenon’s capsule. A pocket is formed by blunt dissection of Tenon’s
capsule from the episclera. The body of the implant was inserted into
the pocket with the leading edge of the device around 8-10 mm from
the limbus. Results: No post-operative complications were detected
in the operated eyes except a persistent corneal edema occupied the
upper half of the cornea in one case. Hyphaema was very mild and
seen only in two cases which resolved quickly two days after surgery.
Endoscopical evaluation for the operated eyes revealed a normal
ocular fundus with clearly visible optic papilla, tapetum and retinal
blood vessels. No evidence of hemorrhage, infection, adhesions or
retinal abnormalities was detected. Conclusion: Ahmed glaucoma
valve is safe and effective implant for treatment of uveitic glaucoma
in dogs.
Abstract: There was a scenario present day that drying of fresh
fruits and vegetables by indirect solar drying by using mechanical
device; hence, an effort was made to develop a small scale solar
tunnel dryer (STD). Drying of spinach is carried out to analyze the
performance of the dryer and to study its drying characteristics. To
evaluate the performance of dryer the independent variables were
selected as air flow rate, loading density and shade net while collector
efficiency, drying efficiency, overall efficiency and specific energy
consumption were selected as responses during performing the
experiments. The spinach was dried from initial moisture content
88.21-94.04% (w.b.) to final moisture content 3.50-5.13% (w.b.). The
drying time considerably reduced as compared to open sun drying of
spinach as sun drying took 15 h for drying. The average collector
efficiency, drying efficiency and overall efficiency were in the range
28.73-61.15%, 11.63% to 22.13%, and 7.61-14.66%, respectively.
Abstract: The nutritional composition and hypoglycaemic effect
of crackers produced from blend of sprouted pigeon pea, unripe
plantain and brewers’ spent grain and fed to Alloxan induced diabetic
rat was investigated. Crackers were produced from different blends of
sprouted pigeon pea, unripe plantain and brewers’ spent grain. The
crackers were evaluated for proximate composition, amino acid
profile and antinutritional factors. Blood glucose levels of normal and
diabetic rats fed with the control sample and different formulations of
cracker were measured. The protein content of the samples were
significantly different (p
Abstract: This research work is an experimental study, through
development of an adhesive from Prosopis africana endosperm. The
prosopis seed for this work were obtained from Enugu State in the
South East part of Nigeria. The seeds were prepared by separating the
endosperm from the seed coat and cotyledon. Three methods were
used to separate them, which are acidic method, roasting method and
boiling method. 20g of seed were treated with different
concentrations (25, 40, 55, 70, and 85% w/w) at 100°C and constant
time (30 minutes), under continuous stirring with magnetic stirrer.
Also 20g of seed were treated with sulphuric acid of concentrations
40% w/w at 100°C with different time (10, 15, 20, 25, 30 minutes),
under continuous stirring with magnetic stirrer. Finally, 20g of seed
were treated with sulphuric acid of concentrations 40% w/w at
different temperature (20°C, 40°C, 60°C, 80°C, and 100°C) with
constant time (30 minutes), under continuous stirring with magnetic
stirrer. The whole endosperm extracted was adhesive. The physical
properties of the adhesive were determined (appearance, odour, taste,
solubility, pH, size, and binding strength). The percentage of the
adhesive yield makes the commercialization of the seed in Nigeria
possible and profitable. The very high viscosity attained at low
concentrations makes prosopis adhesive an excellent thickener in the
food industry.
Abstract: In the present research, whole meal barley flour
(WBF) was supplemented with gelatinized corn flour (GCF) in 0 and
30%. Whole meal wheat flour (WWF) was mixed with defatted rice
bran (DRB) to produce 0, 20, 25, and 30% replacement levels.
Rheological properties of dough were studied. Thermal properties
and starch crystallinity of flours were evaluated. Flat bread, balady
bread and pie were prepared from the different flour blends. The
different bakeries were sensory evaluated. Color of raw materials and
crust of bakery products were determined. Nutrients contents of raw
flours and food products were assessed. Results showed that addition
of GCF to WBF increased the viscosity and falling number of the
produced dough. Water absorption, dough development time and
dough stability increased with increasing the level of DRB in dough
while, weakening and mixing tolerance index decreased.
Extensibility and energy decreased, while, resistance to extension
increased as DRB level increased. Gelatinized temperature of WWF,
WBF, GCF, and DRB were 13.26, 35.09, 28.33, and 39.63,
respectively. Starch crystallinity was affected when DRB was added
to WWF. The highest protein content was present in balady bread
made from 70% WWF and 30% DRB. The highest calcium,
phosphorus, and potassium levels were present in products made
from 100% WBF. Sensory attributes of the products were slightly
affected by adding DRB and GCF. Conclusion: Addition of DRB or
GCF to WWF or WBF, respectively affect the physical, chemical,
rheological and sensory properties of balady bread, flat bread, and pie
while improved their nutritive values.
Abstract: Value addition to agricultural produce is of possible
potential in reducing poverty, improving food security and
malnutrition, therefore the need to develop small and microenterprises
of sweet potato production. A study was carried out in Nigeria to determine the acceptability
of blends sweet potato (Ipomea batatas) and commodities yellow
maize (Zea mays), millet (Pennisetum glaucum), soybean (Glycine
max), bambara groundnut (Vigna subterranean), guinea corn
(Sorghum vulgare), wheat (Triticum aestivum), and roselle (Hibiscus
sabdariffa) through sensory evaluation. Sweet potato (Ipomea batatas) roots were processed using two
methods: oven and sun drying. The blends were also assessed in
terms of functional, chemical and color properties. Most acceptable blends include BAW (80:20 of sweet
potato/wheat), BBC (80:20 of sweet potato/guinea corn), AAB (60:40
of sweet potato/guinea corn), YTE (100% soybean), TYG (100%
sweet potato), KTN (100% wheat flour), XGP (80:20 of sweet
potato/soybean), XAX (60:40 of sweet potato/wheat), LSS (100%
Roselle), CHK (100% Guinea corn), and ABC (60:40% of sweet
potato/ yellow maize). In addition, carried out chemical analysis
revealed that sweet potato has high percentage of vitamins A and C,
potassium (K), manganese (Mn), calcium (Ca), magnesium (Mg) and
iron (Fe) and fibre content. There is also an increase of vitamin A and
Iron in the blended products.
Abstract: This study investigated the effects of thermal
treatment on Tualang honey sample in terms of honey colour and
heat-induced small metabolites. The heating process was carried out
in a temperature controlled water batch at 90oC for 4 hours. The
honey samples were put in cylinder tubes with the dimension of 1 cm
diameter and 10 cm length for homogenous heat transfer. The results
found that the thermal treatment produced not only
hydroxylmethylfurfural, but also other harmful substances such as
phthalic anhydride and radiolytic byproducts. The degradation of
honey protein was due to the detection of free amino acids such as
cysteine and phenylalanine in heat-treated honey samples. Sugar
dehydration was also occurred because fragmented di-galactose was
identified based on the presence of characteristic ions in the mass
fragmentation pattern. The honey colour was found getting darker as
the heating duration was increased up to 4 hours. Approximately, 60
mm PFund of increment was noticed for the honey colour with the
colour change rate of 14.8 mm PFund per hour. Based on the
principal component analysis, the score plot clearly shows that the
chemical profile of Tualang honey was significantly altered after 2
hours of heating at 90oC.
Abstract: Lead being a toxic heavy metal that mankind is
exposed to the highest levels of this metal. There are different sources
of environmental pollution with lead as lead alkyl additives in petrol
and manufacturing processes. The contaminated atmosphere in urban
and industrial areas by lead in Egypt may lead to the contamination
of foods beside the other different sources. The present investigation
studied the risk assessment of lead in some Egyptian edible
vegetables and fruits collected from different environments in Greater
Cairo Governorate, i.e. industrial, heavy traffic and rural areas. A
total of 325 leafy and fruity vegetables and fruits samples belonging
to 11, 6 and 4 different species, respectively were randomly collected
from markets of the three main models. Data indicated the variation
of lead levels in different three areas. The highest levels of lead were
detected in the samples collected from industrial and traffic areas.
However, the lowest levels were found in the rural areas. It could be
concluded that determination of lead levels in foods from different
localities and environments at regularly is very important.