Surface Characteristics of Bacillus megaterium and Its Adsorption Behavior onto Dolomite
Surface characteristics of Bacillus megaterium strain
were investigated; zeta potential, FTIR and contact angle were
measured. Surface energy components including Lifshitz-van der
Waals, Hamaker constant, and acid/base components (Lewis
acid/Lewis base) were calculated from the contact angle data. The
results showed that the microbial cells were negatively charged over
all pH regions with high values at alkaline region. A hydrophilic
nature for the strain was confirmed by contact angle and free energy
of adhesion between microbial cells. Adsorption affinity of the strain
toward dolomite was studied at different pH values. The results
showed that the cells had a high affinity to dolomite at acid pH
comparing to neutral and alkaline pH. Extended DLVO theory was
applied to calculate interaction energy between B. megaterium cells
and dolomite particles. The adsorption results were in agreement with
the results of Extended DLVO approach. Surface changes occurred
on dolomite surface after the bio-treatment were monitored; contact
angle decreased from 69° to 38° and the mineral’s floatability
decreased from 95% to 25% after the treatment.
[1] Mohsen Farahat, Tsuyoshi Hirajima, Keiko Sasaki, Yuuki Aiba,
Katsumi Doi, Adsorption of SIP E. coli onto quartz and its applications
in froth flotation, Minerals Engineering, Volume 21, Issue 5, April 2008,
Pages 389–395.
[2] Mohsen Farahat, Tsuyoshi Hirajima, Keiko Sasaki, Katsumi Doi,
Adhesion of Escherichia coli onto quartz, hematite and corundum:
extended DLVO theory and flotation behavior, Colloids and Surfaces B:
Biointerfaces, Volume 74, Issue 1, 1 November 2009, Pages 140-149.
[3] Huifen Yang, Qiongyao Tang, Chuanlong Wang, Jinlong Zhang,
Flocculation and flotation response of Rhodococcus erythropolis to pure
minerals in hematite ores, Minerals Engineering, Volume 45, May 2013,
Pages 67–72.
[4] Leslie Y. Lopez, Antonio G. Merma, Mauricio L. Torem, Gabriela H.
Pino, Fundamental aspects of hematite flotation using the bacterial strain
Rhodococcus ruber as bioreagent, Minerals Engineering, Volume 75, 1
May 2015, Pages 63–69.
[5] Partha Patra, K.A. Natarajan, Surface chemical studies on selective
separation of pyrite and galena in the presence of bacterial cells and
metabolic products of Paenibacillus polymyxa, Journal of Colloid and
Interface Science, Volume 298, Issue 2, 15 June 2006, Pages 720–729.
[6] M.N Chandraprabha, K.A Natarajan, P Somasundaran, Selective
separation of arsenopyrite from pyrite by biomodulation in the presence
of Acidithiobacillus ferrooxidans, Journal of Colloid and Interface
Science, Volume 276, Issue 2, 15 August 2004, Pages 323–332.
[7] Mohsen Farahat, Tsuyoshi Hirajima, Keiko Sasaki, Adhesion of
Ferroplasma acidiphilum onto pyrite calculated from the extended
DLVO theory using the van Oss–Good–Chaudhury approach, Journal of
Colloid and Interface Science, Volume 349, Issue 2, 15 September 2010,
Pages 594–601.
[8] Mohsen Farahat, Tsuyoshi Hirajima, Hydrophilicity of Ferroplasma
acidiphilum and its effect on the depression of pyrite, Minerals
Engineering, Volumes 36–38, October 2012, Pages 242–247.
[9] Tsuyoshi Hirajima, Yuki Aiba, Mohsen Farahat, Naoko Okibe, Keiko
Sasaki, Takehiko Tsuruta, Katsumi Doi, Effect of microorganisms on
flocculation of quartz, International Journal of Mineral Processing,
Volumes 102–103, 25 January 2012, Pages 107–111.
[10] Sharma, P.K., Hanumantha Rao, K., Forssberg, K.S.E., Natarajan, K.A.,
Surface chemical characterization of Paenibacillus polymyxa before and
after adaptation to sulfide minerals, International journal of Mineral
Processing, 62 (2001) 3-25.
[11] Rijnaarts, H.M., Norde, W, Lyklema, J., Zehnder, A, The isoelectric
point of bacteria as an indicator for the presence of cell surface polymers
that inhibit adhesion. Colloids and Surfaces B: Biointerfaces, 4 (1995)
191-197.
[12] Sharmaa, P.K., Dasb, Hanumantha Raoa, A., K., Forssberg, K.S.E.,
Surface characterization of Acidithiobacillus ferrooxidans cells grown
under different conditions, Hydrometallurgy, 71 (2003) 285–292.
[13] Van der Mei, H. C.; Bos, R.; Busscher, H. J, A reference guide to
microbial cell surface hydrophobicity based on contact angles, Colloids
and Surfaces B: Biointerfaces, 11 (1998) 213-221.
[14] Patricia S. Vary, Rebekka Biedendieck, Tobias Fuerch, Friedhelm
Meinhardt, Manfred Rohd, Wolf-Dieter, Deckwer, Dieter Jahn, Bacillus
megaterium—from simple soil bacterium to industrial protein
production host, Appl Microbiol Biotechnol (2007) 76:957–967.
[15] Beveridge TJ., Ultrastructure, chemistry, and function of the bacterial
wall. International Review of Cytology, 1981;72:229-317.
[16] Wei Jiang, Anuradha Saxena, Bongkeun Song, Bess B. Ward, Terry J.
Beveridge, Satish C. B. Myneni, Elucidation of Functional Groups on
Gram-Positive and Gram-Negative Bacterial Surfaces Using Infrared
Spectroscopy, Langmuir 2004, 20, 11433-11442.
[17] B. Vasanthakumar, H. Ravishankar, S. Subramanian, Microbially
induced selective flotation of sphalerite from galena using mineraladapted
strains of Bacillus megaterium, Colloids and Surfaces B:
Biointerfaces, Volume 112, 1 December 2013, Pages 279–286.
[18] B. Vasanthakumar, H. Ravishankar, S. Subramanian, Basic studies on
the role of components of Bacillus megaterium as flotation biocollectors
in sulphide mineral separation, Appl Microbiol Biotechnol. 2014
Mar;98(6):2719-28.
[19] Busscher, H.J., Weerkamp, A. H., Van der Mei H.C., van pelt A.W.J, de
jong H.P., Arends J., Measurements of surface free energy of bacterial
cell surfaces and its relevance to adhesion, Applied environmental
microbiology, 48(5) (1984) 980-983.
[20] Van Oss, C.J., Gillman, C.F., Neumann, A.W., 1975, Chapter 2, In:
Phagocytic engulfment and cell adhesiveness, Isenberg, H.D., Eds.,
Dekker, New York, 13-16 Van der Mei, H.C., Bos, R., Busscher, H.J., A
reference guide to microbial cell surface hydrophobicity based on
contact angles, Colloid and Surfaces B: Biointerfaces, 11 (1998) 213-
221 [21] Van der Mei, H.C., Bos, R., Busscher, H.J., A reference guide to
microbial cell Surface hydrophobicity based on contact angles, Colloids
and surfaces B: Biointerfaces, 11(1998) 213-221.
[22] Bellamy, L.J., In Surface characterization of Acidithiobacillus
ferrooxidans cells grown under different conditions, Sharmaa, P.K.,
Dasb, Hanumantha Raoa, A., K., Forssberg, K.S.E., Hydrometallurgy 71
(2003) 285–292.
[23] Twardowski, J., Anzenbacher, P., In Surface characterization of
Acidithiobacillus ferrooxidans cells grown under different conditions,
Sharmaa, P.K., Dasb, Hanumantha Raoa, A., K., Forssberg, K.S.E.,
Hydrometallurgy 71 (2003) 285–292.
[24] Sharma, P.K., Hanumantha Rao, K., Forssberg, K.S.E., Natarajan, K.A.,
Surface chemical characterization of Paenibacillus polymyxa before and
after adaptation to sulfide minerals, International journal of Mineral
Processing, 62 (2001) 3-25.
[25] Dudley William Thompson, Pamela Gillian Pownall, Surface electrical
properties of calcite, Journal of Colloid and Interface Science, Volume
131, Issue 1, August 1989, Pages 74–82.
[26] Mélanie Davranche, Sandrine Beaufreton, Jean-Claude Bollinger,
Influence of Carbonates on the Surface Charge of a Natural Solid,
Journal of Colloid and Interface Science, volume 249, Issue 1, 1 May
2002, Pages 113–118.
[27] Daniel S Cicerone, Alberto E Regazzoni, Miguel A Blesa, Electrokinetic
properties of the calcite/water interface in the presence of magnesium
and organic matter, Journal of Colloid and Interface Science, volume
154, Issue 2, December 1992, Pages 423–433.
[28] Reda Marouf, Kheira Marouf-Khelifa, Jacques Schott, Amine Khelifa,
Zeta potential study of thermally treated dolomite samples in electrolyte
solutions, Microporous and Mesoporous Materials 122 (2009) 99–104.
[29] Van Oss, C. J. Hydrophobicity of biosurfaces-Origin, quantitative
determination and interaction energies, Colloids and Surfaces B:
Biointerfaces, 5 (1995) 91-110.
[30] Van Oss, C.J., Interfacial forces in aqueous media, Marcel Dekker, New
York, (1994).
[31] Brown, Derick G., Effects of Nonionic Surfactants on the Cell Surface
Hydrophobicity and Apparent Hamaker Constant of a Sphingomonas sp,
Environmental Science and Technology, 40 (1) (2006) 195 -201.
[32] Vernhet, A., Bellon-Fontaine M.N., Role of bentonites in the prevention
of Saccharomyces cerevisiae adhesion to solid surfaces, Colloids and
Surfaces B: Biointerfaces 3 (I995) 255-262.
[33] Dopson, M., E.B. Lindstrom, Potential role of Thiobacillus caldus in
arsenopyrite bioleaching, Applied Environmental Microbiology, 65
(1999) 36-40.
[1] Mohsen Farahat, Tsuyoshi Hirajima, Keiko Sasaki, Yuuki Aiba,
Katsumi Doi, Adsorption of SIP E. coli onto quartz and its applications
in froth flotation, Minerals Engineering, Volume 21, Issue 5, April 2008,
Pages 389–395.
[2] Mohsen Farahat, Tsuyoshi Hirajima, Keiko Sasaki, Katsumi Doi,
Adhesion of Escherichia coli onto quartz, hematite and corundum:
extended DLVO theory and flotation behavior, Colloids and Surfaces B:
Biointerfaces, Volume 74, Issue 1, 1 November 2009, Pages 140-149.
[3] Huifen Yang, Qiongyao Tang, Chuanlong Wang, Jinlong Zhang,
Flocculation and flotation response of Rhodococcus erythropolis to pure
minerals in hematite ores, Minerals Engineering, Volume 45, May 2013,
Pages 67–72.
[4] Leslie Y. Lopez, Antonio G. Merma, Mauricio L. Torem, Gabriela H.
Pino, Fundamental aspects of hematite flotation using the bacterial strain
Rhodococcus ruber as bioreagent, Minerals Engineering, Volume 75, 1
May 2015, Pages 63–69.
[5] Partha Patra, K.A. Natarajan, Surface chemical studies on selective
separation of pyrite and galena in the presence of bacterial cells and
metabolic products of Paenibacillus polymyxa, Journal of Colloid and
Interface Science, Volume 298, Issue 2, 15 June 2006, Pages 720–729.
[6] M.N Chandraprabha, K.A Natarajan, P Somasundaran, Selective
separation of arsenopyrite from pyrite by biomodulation in the presence
of Acidithiobacillus ferrooxidans, Journal of Colloid and Interface
Science, Volume 276, Issue 2, 15 August 2004, Pages 323–332.
[7] Mohsen Farahat, Tsuyoshi Hirajima, Keiko Sasaki, Adhesion of
Ferroplasma acidiphilum onto pyrite calculated from the extended
DLVO theory using the van Oss–Good–Chaudhury approach, Journal of
Colloid and Interface Science, Volume 349, Issue 2, 15 September 2010,
Pages 594–601.
[8] Mohsen Farahat, Tsuyoshi Hirajima, Hydrophilicity of Ferroplasma
acidiphilum and its effect on the depression of pyrite, Minerals
Engineering, Volumes 36–38, October 2012, Pages 242–247.
[9] Tsuyoshi Hirajima, Yuki Aiba, Mohsen Farahat, Naoko Okibe, Keiko
Sasaki, Takehiko Tsuruta, Katsumi Doi, Effect of microorganisms on
flocculation of quartz, International Journal of Mineral Processing,
Volumes 102–103, 25 January 2012, Pages 107–111.
[10] Sharma, P.K., Hanumantha Rao, K., Forssberg, K.S.E., Natarajan, K.A.,
Surface chemical characterization of Paenibacillus polymyxa before and
after adaptation to sulfide minerals, International journal of Mineral
Processing, 62 (2001) 3-25.
[11] Rijnaarts, H.M., Norde, W, Lyklema, J., Zehnder, A, The isoelectric
point of bacteria as an indicator for the presence of cell surface polymers
that inhibit adhesion. Colloids and Surfaces B: Biointerfaces, 4 (1995)
191-197.
[12] Sharmaa, P.K., Dasb, Hanumantha Raoa, A., K., Forssberg, K.S.E.,
Surface characterization of Acidithiobacillus ferrooxidans cells grown
under different conditions, Hydrometallurgy, 71 (2003) 285–292.
[13] Van der Mei, H. C.; Bos, R.; Busscher, H. J, A reference guide to
microbial cell surface hydrophobicity based on contact angles, Colloids
and Surfaces B: Biointerfaces, 11 (1998) 213-221.
[14] Patricia S. Vary, Rebekka Biedendieck, Tobias Fuerch, Friedhelm
Meinhardt, Manfred Rohd, Wolf-Dieter, Deckwer, Dieter Jahn, Bacillus
megaterium—from simple soil bacterium to industrial protein
production host, Appl Microbiol Biotechnol (2007) 76:957–967.
[15] Beveridge TJ., Ultrastructure, chemistry, and function of the bacterial
wall. International Review of Cytology, 1981;72:229-317.
[16] Wei Jiang, Anuradha Saxena, Bongkeun Song, Bess B. Ward, Terry J.
Beveridge, Satish C. B. Myneni, Elucidation of Functional Groups on
Gram-Positive and Gram-Negative Bacterial Surfaces Using Infrared
Spectroscopy, Langmuir 2004, 20, 11433-11442.
[17] B. Vasanthakumar, H. Ravishankar, S. Subramanian, Microbially
induced selective flotation of sphalerite from galena using mineraladapted
strains of Bacillus megaterium, Colloids and Surfaces B:
Biointerfaces, Volume 112, 1 December 2013, Pages 279–286.
[18] B. Vasanthakumar, H. Ravishankar, S. Subramanian, Basic studies on
the role of components of Bacillus megaterium as flotation biocollectors
in sulphide mineral separation, Appl Microbiol Biotechnol. 2014
Mar;98(6):2719-28.
[19] Busscher, H.J., Weerkamp, A. H., Van der Mei H.C., van pelt A.W.J, de
jong H.P., Arends J., Measurements of surface free energy of bacterial
cell surfaces and its relevance to adhesion, Applied environmental
microbiology, 48(5) (1984) 980-983.
[20] Van Oss, C.J., Gillman, C.F., Neumann, A.W., 1975, Chapter 2, In:
Phagocytic engulfment and cell adhesiveness, Isenberg, H.D., Eds.,
Dekker, New York, 13-16 Van der Mei, H.C., Bos, R., Busscher, H.J., A
reference guide to microbial cell surface hydrophobicity based on
contact angles, Colloid and Surfaces B: Biointerfaces, 11 (1998) 213-
221 [21] Van der Mei, H.C., Bos, R., Busscher, H.J., A reference guide to
microbial cell Surface hydrophobicity based on contact angles, Colloids
and surfaces B: Biointerfaces, 11(1998) 213-221.
[22] Bellamy, L.J., In Surface characterization of Acidithiobacillus
ferrooxidans cells grown under different conditions, Sharmaa, P.K.,
Dasb, Hanumantha Raoa, A., K., Forssberg, K.S.E., Hydrometallurgy 71
(2003) 285–292.
[23] Twardowski, J., Anzenbacher, P., In Surface characterization of
Acidithiobacillus ferrooxidans cells grown under different conditions,
Sharmaa, P.K., Dasb, Hanumantha Raoa, A., K., Forssberg, K.S.E.,
Hydrometallurgy 71 (2003) 285–292.
[24] Sharma, P.K., Hanumantha Rao, K., Forssberg, K.S.E., Natarajan, K.A.,
Surface chemical characterization of Paenibacillus polymyxa before and
after adaptation to sulfide minerals, International journal of Mineral
Processing, 62 (2001) 3-25.
[25] Dudley William Thompson, Pamela Gillian Pownall, Surface electrical
properties of calcite, Journal of Colloid and Interface Science, Volume
131, Issue 1, August 1989, Pages 74–82.
[26] Mélanie Davranche, Sandrine Beaufreton, Jean-Claude Bollinger,
Influence of Carbonates on the Surface Charge of a Natural Solid,
Journal of Colloid and Interface Science, volume 249, Issue 1, 1 May
2002, Pages 113–118.
[27] Daniel S Cicerone, Alberto E Regazzoni, Miguel A Blesa, Electrokinetic
properties of the calcite/water interface in the presence of magnesium
and organic matter, Journal of Colloid and Interface Science, volume
154, Issue 2, December 1992, Pages 423–433.
[28] Reda Marouf, Kheira Marouf-Khelifa, Jacques Schott, Amine Khelifa,
Zeta potential study of thermally treated dolomite samples in electrolyte
solutions, Microporous and Mesoporous Materials 122 (2009) 99–104.
[29] Van Oss, C. J. Hydrophobicity of biosurfaces-Origin, quantitative
determination and interaction energies, Colloids and Surfaces B:
Biointerfaces, 5 (1995) 91-110.
[30] Van Oss, C.J., Interfacial forces in aqueous media, Marcel Dekker, New
York, (1994).
[31] Brown, Derick G., Effects of Nonionic Surfactants on the Cell Surface
Hydrophobicity and Apparent Hamaker Constant of a Sphingomonas sp,
Environmental Science and Technology, 40 (1) (2006) 195 -201.
[32] Vernhet, A., Bellon-Fontaine M.N., Role of bentonites in the prevention
of Saccharomyces cerevisiae adhesion to solid surfaces, Colloids and
Surfaces B: Biointerfaces 3 (I995) 255-262.
[33] Dopson, M., E.B. Lindstrom, Potential role of Thiobacillus caldus in
arsenopyrite bioleaching, Applied Environmental Microbiology, 65
(1999) 36-40.
@article{"International Journal of Biological, Life and Agricultural Sciences:71966", author = "Mohsen Farahat and Tsuyoshi Hirajima", title = "Surface Characteristics of Bacillus megaterium and Its Adsorption Behavior onto Dolomite", abstract = "Surface characteristics of Bacillus megaterium strain
were investigated; zeta potential, FTIR and contact angle were
measured. Surface energy components including Lifshitz-van der
Waals, Hamaker constant, and acid/base components (Lewis
acid/Lewis base) were calculated from the contact angle data. The
results showed that the microbial cells were negatively charged over
all pH regions with high values at alkaline region. A hydrophilic
nature for the strain was confirmed by contact angle and free energy
of adhesion between microbial cells. Adsorption affinity of the strain
toward dolomite was studied at different pH values. The results
showed that the cells had a high affinity to dolomite at acid pH
comparing to neutral and alkaline pH. Extended DLVO theory was
applied to calculate interaction energy between B. megaterium cells
and dolomite particles. The adsorption results were in agreement with
the results of Extended DLVO approach. Surface changes occurred
on dolomite surface after the bio-treatment were monitored; contact
angle decreased from 69° to 38° and the mineral’s floatability
decreased from 95% to 25% after the treatment.", keywords = "Bacillus megaterium, surface modification, flotation,
dolomite, adhesion energy.", volume = "9", number = "11", pages = "1175-8", }
