Abstract: Strong anthropogenic impact has uncontrolled
consequences on the nature of the soil. Hence, up-to-date sustainable
methods of soil state improvement are essential. Investigators provide
the evidence that biochar can positively effects physical, chemical,
and biological soil properties and the abundance of mycorrhizal fungi
which are in the focus of this study. The main aim of the present
investigation is to demonstrate the effect of two types of plant growth
promoting bacteria (PGPB) inoculums along with the beech wood
biochar and mineral N additives on mycorrhizal colonization.
Experiment has been set up in laboratory conditions with containers
filled with arable soil from the protection zone of the main water
source “Brezova nad Svitavou”. Lactuca sativa (lettuce) has been
selected as a model plant. Based on the obtained data, it can be
concluded that mycorrhizal colonization increased as the result of
combined influence of biochar and PGPB inoculums amendment. In
addition, correlation analyses showed that the numbers of main
groups of cultivated bacteria were dependent on the degree of
mycorrhizal colonization.
Abstract: Escherichia coli (E. coli) is the most isolated bacteria
from blood circulation of septicemic calves. Given the prevalence of
septicemia in animals and its economic importance in veterinary
practice, better understanding of changes in clinical signs following
disease, may contribute to early detection of disorder. The present
study has been carried out to detect changes of clinical signs in
induced sepsis in calves with E. coli. Colisepticemia has been
induced in 10 twenty-day old healthy Holstein- Frisian calves with
intravenous injection of 1.5 X 109 colony forming units (cfu) of
O111:H8 strain of E. coli. Clinical signs including rectal temperature,
heart rate, respiratory rate, shock, appetite, sucking reflex, feces
consistency, general behavior, dehydration and standing ability were
recorded in experimental calves during 24 hours after induction of
colisepticemia. Blood culture was also carried out from calves four
times during experiment. ANOVA with repeated measure is used to
see changes of calves’ clinical signs to experimental colisepticemia,
and values of P≤ 0.05 was considered statistically significant. Mean
values of rectal temperature and heart rate as well as median values
of respiratory rate, appetite, suckling reflex, standing ability and feces
consistency of experimental calves increased significantly during
study (P 0.05). The
results of present study showed that total score of clinical signs in
calves with experimental colisepticemia increased significantly,
although score of some clinical signs such as shock did not change
significantly.
Abstract: To evaluate the effect of intramuscular administration
of vitamin A in pregnant dairy cows during late stages of pregnancy,
on vitamin A status of neonatal calves, a total of 30 cows were
randomly selected and divided into three groups; two treatment
groups and one control group. Single intramuscular injection of
2000000 IU vitamin A; was carried in 10 dairy cows at 7 months of
pregnancy (group 1). In second group of treated animals (10 cows)
the injection was performed in 8 months of pregnancy (group 2). Ten
pregnant dairy cows were received saline injection as placebo and
selected as control group. Blood samples were collected from
experimental dairy cows at 7 and 8 months of pregnancy as well as
their newborn calves’ pre and after colostrum intake. There was no
significant difference between vitamin A and β-carotene
concentration of dairy cows of three groups in two last months of
pregnancy (P> 0.05). Vitamin A concentration of calves of two
treatment groups before and after receiving of colostrum were
significantly higher than that in control group (P< 0.05). There was
no significant difference between serum concentrations of vitamin A
in calves of two treated groups (P> 0.05). β-Carotene concentration
of serum samples of dairy cows and neonatal calves of three groups
were not significantly different as compared with together. From
results of the present study it can be concluded that single injection of
vitamin A during at 7 or 8 month of pregnancy can significantly
increase level of vitamin A in their colostrum and neonatal calves.
Abstract: This study evaluated the acute toxicity and tissue
distribution of intravenously administered gold nanoparticles
(AuNPs) in male rabbits. Rabbits were exposed to single dose of
AuNPs (300 μg/ kg). Toxic effects were assessed via general
behavior, hematological parameters, serum biochemical parameters,
and histopathological examination of various rabbits’ organs.
Inductively coupled plasma–mass spectrometry (ICP-MS) was used
to determine gold concentrations in tissue samples collected at
predetermined time intervals. After one week, AuNPs exerted no
obvious acute toxicity in rabbits. However, inflammatory reactions
were observed in liver, lungs and kidneys accompanied with mild
absolute neutrophilia and significant monocytosis. The highest gold
levels were found in the spleen and liver followed by lungs, and
kidneys. These results indicated that AuNPs could be distributed
extensively to various tissues in the body, but primarily in the spleen
and liver.
Abstract: Laura Island, which is located about 50 km away from
downtown, is a source of water supply in Majuro atoll, which is the
capital of the Republic of the Marshall Islands. Low and flat Majuro
atoll has neither river nor lake. It is very important for Majuro atoll to
ensure the conservation of its water resources. However, upconing,
which is the process of partial rising of the freshwater-saltwater
boundary near the water-supply well, was caused by the excess
pumping from it during the severe drought in 1998. Upconing will
make the water usage of the freshwater lens difficult. Thus,
appropriate water usage is required to prevent up coning in the
freshwater lens because there is no other water source during drought. Numerical simulation of water usage applying SEAWAT model
was conducted at the central part of Laura Island, including the water
supply well, which was affected by upconing. The freshwater lens was
created as a result of infiltration of consistent average rainfall. The lens
shape was almost the same as the one in 1985. 0 of monthly rainfall
and variable daily pump discharge were used to calculate the
sustainable pump discharge from the water supply well. Consequently,
the total amount of pump discharge was increased as the daily pump
discharge was increased, indicating that it needs more time to recover
from upconing. Thus, a pump standard to reduce the pump intensity is
being proposed, which is based on numerical simulation concerning
the occurrence of the up-coning phenomenon in Laura Island during
the drought.
Abstract: Biochar can be produced from the waste matter and its
application has been associated with returning of carbon in large
amounts into the soil. The impacts of this material on physical and
chemical properties of soil have been described. The biggest part of
the research work is dedicated to the hypothesis of this material’s
toxic effects on the soil life regarding its effect on the soil biological
component. At present, it has been worked on methods which could
eliminate these undesirable properties of biochar. One of the
possibilities is to mix biochar with organic material, such as compost,
or focusing on the natural processes acceleration in the soil. In the
experiment has been used as the addition of compost as well as the
elimination of toxic substances by promoting microbial activity in
aerated water environment. Biochar was aerated for 7 days in a
container with a volume of 20 l. This way modified biochar had six
times higher biomass production and reduce mineral nitrogen
leaching. Better results have been achieved by mixing biochar with
compost.
Abstract: Gladiolus is an important cash crop and is grown
mainly for its elegant spikes. Traditionally the gladiolus corms are
planted manually which is very tedious, time consuming and labor
intensive operation. So far, there is no planter available for planting
of gladiolus corms. With a view to mechanize the planting operation
of this horticultural crop, a prototype of 4-row gladiolus planter was
developed and its performance was evaluated in-situ condition. Cupchain
type metering device was used to place each single gladiolus
corm in furrow at required spacing while planting. Three levels of
corm spacing viz 15, 20 and 25 cm and four levels of forward speed
viz 1.0, 1.5, 2.0 and 2.5 km/h was taken as evaluation parameter for
the planter. The performance indicators namely corm spacing in each
row, coefficient of uniformity, missing index, multiple index, quality
of feed index, number of corms per meter length, mechanical damage
to the corms etc. were determined during the field test. The data was
statistically analyzed using Completely Randomized Design (CRD)
for testing the significance of the parameters. The result indicated
that planter was able to drop the corms at required nominal spacing
with minor variations. The highest deviation from the mean corm
spacing was observed as 3.53 cm with maximum coefficient of
variation as 13.88%. The highest missing and quality of feed indexes
were observed as 6.33% and 97.45% respectively with no multiples.
The performance of the planter was observed better at lower forward
speed and wider corm spacing. The field capacity of the planter was
found as 0.103 ha/h with an observed field efficiency of 76.57%.
Abstract: The aim of the study was to investigate the effect of
Saccharomyces cerevisiae (SC) live yeast culture on microbial
protein supply to small intestine in Kivircik male yearlings when fed
with different ratio of forage and concentrate diets. Four Kivircik
male yearlings with permanent rumen canula were used in the
experiment. The treatments were allocated to a 4x4 Latin square
design. Diet I consisted of 70% alfalfa hay and 30% concentrate, Diet
II consisted of 30% alfalfa hay and 70% concentrate, Diet I and II
were supplemented with a SC. Daily urine was collected and stored at
-20°C until analysis. Calorimetric methods were used for the
determination of urinary allantoin and creatinine levels. The
estimated microbial N supply to small intestine for Diets I, I+SC, II
and II+SC were 2.51, 2.64, 2.95 and 3.43 g N/d respectively.
Supplementation of Diets I and II with SC significantly affected the
allantoin levels in μmol/W0.75 (p
Abstract: Selenium is an-antioxidant which is important for
human health enters food chain through crops. In Kenya Zea mays is
consumed by 96% of population hence is a cheap and convenient
method to provide selenium to large number of population. Several
soil factors are known to have antagonistic effects on selenium
speciation hence the uptake by Zea mays. There are no studies in
Kenya that has been done to determine the effects of soil
characteristics (pH, Tcarbon, CEC, Eh) affect accumulation of
selenium in Zea mays grains in Maize Belt in Kenya. About 100 Zea mays grain samples together with 100 soil samples
were collected from the study site put in separate labeled Ziplocs and
were transported to laboratories at room temperature for analysis.
Maize grains were analyzed for selenium while soil samples were
analyzed for pH, Cat Ion Exchange Capacity, total carbon, and
electrical conductivity. The mean selenium in Zea mays grains varied from 1.82 ± 0.76
mg/Kg to 11±0.86 mg/Kg. There was no significant difference
between selenium levels between different grain batches {χ (Df =76)
= 26.04 P= 1.00} The pH levels varied from 5.43± 0.58 to 5.85±
0.32. No significant correlations between selenium in grains and soil
pH (Pearson’s correlations = - 0.143), and between selenium levels in
grains and the four (pH, Tcarbon, CEC, Eh) soil chemical
characteristics {F (4,91) = 0.721 p = 0.579} was observed. It can be concluded that the soil chemical characteristics in the
study site did not significantly affect the accumulation of native
selenium in Zea mays grains.
Abstract: This research was made during the 2015 growing
periods in the trial filed of "Research Station for Department of Field
Crops, Agricultural Faculty, Selcuk University" according to
“Randomized Blocks Design” with 3 replications. Research material
was the following pea lines; PS16, PS18, PS21, PS23, PS24, PS25,
PS36, PS47, PS49, PS51, PS54, PS58, PS67, PS69, PS71, PS73,
PS83, PS84, PS87 and PSKY and three cultivars and other 2
commercial varieties named as Bolero, Rondo and Ultrello. Some
agronomical characteristics such as plant height (cm) number of pod
per plant number of seed per pod number of seed per plant 100 seed
weight (g) and seed yield (kg ha-1) were determined. The highest seed
yield was obtained 2727.0 kg ha-1 in the PS71 line and the lowest
value was obtained 1238.0 kg ha-1 in the commercial variety of
Bolero. Results of the research implicated that the new developed
lines were superior compared with the control (commercial) varieties
by means of most of the characteristics. Nevertheless, similar
researches should be continued in different locations and years.
Abstract: The underutilization of biomass resources in the
Philippines, combined with its growing population and the rise in
fossil fuel prices confirms demand for alternative energy sources. The
goal of this paper is to provide a comparison of MODIS-based and
Landsat-based agricultural land cover maps when used in the
estimation of rice hull’s available energy potential. Biomass resource
assessment was done using mathematical models and remote sensing
techniques employed in a GIS platform.
Abstract: This study aims to examine the sensory quality of
meatballs made from Balinese beef and buffalo meat after the
addition of smoke powder prior to storage at the temperatures of 2-
5°C for 7 days. This study used meat from Longissimus dorsi muscle
of male Balinese cattle aged 3 years and of male buffalo aged 5 years
as the main raw materials, and smoke powder as a binder and
preservative in making meatballs. The study was based on completely
randomized design (CRD) of factorial pattern of 2 x 3 x 2 where
factors 1, 2 and 3 included the types of meat (cattle and buffalo),
types of smoke powder (oven dried, freeze dried and spray dried)
with a level of 2% of the weight of the meat (w/w), and storage
duration (0 and 7 days) with three replications, respectively. The
parameters measured were the meatball sensory quality (scores of
tenderness, firmness, chewing residue, and intensity of flavor). The
results of this study show that each type of meat has produced
different sensory characteristics. The meatballs made from buffalo
meat have higher tenderness and elasticity scores than the Balinese
beef. Meanwhile, the buffalo meatballs have a lower residue
mastication score than the Balinese beef. Each type of smoke
powders has produced a relatively similar sensory quality of
meatballs. It can be concluded that the smoke powder of 2% of the
weight of the meat (w/w) could maintain the sensory quality of the
meatballs for 7 days of storage.
Abstract: The aim of this study is to evaluate the effect of
supplementing broiler starter diet with different levels of an essential
oil blend (EOB) containing capsaicin, carvacrol and cinnamaldehyde
on the performance of broilers. A total of 300 day-old straight-run
Cobb broiler chicks were randomly assigned to three treatments after
7-day group brooding following a completely randomized design
(CRD). Birds assigned in treatment 1 were given starter basal diet
while those in treatments 2 and 3 were given starter basal diet with
400 mg/kg antibiotic growth promoter (AGP) and 150 mg/kg EOB,
respectively, until the 28th day. Basal finisher feed were given for all
the treatments until harvest. Following 37 d feeding, body weight
gain, feed consumption, feed efficiency, dressing percentage,
livability and jejunal villi height were determined. Results showed no
significant differences (P>0.05) in growth performance. However,
villi height and crypt depth was significantly lower for birds fed
EOB.
Abstract: Strong anthropogenic impact has uncontrolled
consequences on the nature of the soil. Hence, up-to-date sustainable
methods of soil state improvement are essential. Investigators provide
the evidence that biochar can positively effects physical, chemical,
and biological soil properties and the abundance of mycorrhizal fungi
which are in the focus of this study. The main aim of the present
investigation is to demonstrate the effect of two types of plant growth
promoting bacteria (PGPB) inoculums along with the beech wood
biochar and mineral N additives on mycorrhizal colonization.
Experiment has been set up in laboratory conditions with containers
filled with arable soil from the protection zone of the main water
source “Brezova nad Svitavou”. Lactuca sativa (lettuce) has been
selected as a model plant. Based on the obtained data, it can be
concluded that mycorrhizal colonization increased as the result of
combined influence of biochar and PGPB inoculums amendment. In
addition, correlation analyses showed that the numbers of main
groups of cultivated bacteria were dependent on the degree of
mycorrhizal colonization.
Abstract: In the vicinity of red sea about 15 fungi species were
isolated from oil contaminated sites. On the basis of aptitude to
degrade the crude oil and DCPIP assay, two fungal isolates were
selected amongst 15 oil degrading strains. Analysis of ITS-1, ITS-2
and amplicon pyrosequencing studies of fungal diversity revealed
that these strains belong to Penicillium and Aspergillus species. Two
strains that proved to be the most efficient in degrading crude oil was
Aspergillus niger (54%) and Penicillium commune (48%) Subsequent
to two weeks of cultivation in BHS medium the degradation rate
were recorded by using spectrophotometer and GC-MS. Hence, it is
cleared that these fungal strains has capability of degradation and can
be utilize for cleaning the Saudi Arabian environment.
Abstract: Anaerobic digestion is a well-known technique for
sustainable energy recovery from sewage sludge. However, sewage
sludge digestion is restricted due to certain factors. Pre-treatment
methods have been established in various publications as a promising
technique to improve the digestibility of the sewage sludge and to
enhance the biogas generated which can be used for energy recovery.
In this study, continuous flow microwave (MW) pre-treatment with
different intensities were compared by using 5 L semi-continuous
digesters at a hydraulic retention time of 27 days. We focused on the
effects of MW at different intensities on the sludge solubilization,
sludge digestibility, and biogas production of the untreated and MW
pre-treated sludge. The MW pre-treatment demonstrated an increase
in the ratio of soluble chemical oxygen demand to total chemical
oxygen demand (sCOD/tCOD) and volatile fatty acid (VFA)
concentration. Besides that, the total volatile solid (TVS) removal
efficiency and tCOD removal efficiency also increased during the
digestion of the MW pre-treated sewage sludge compared to the
untreated sewage sludge. Furthermore, the biogas yield also
subsequently increases due to the pre-treatment effect. A higher MW
power level and irradiation time generally enhanced the biogas
generation which has potential for sustainable energy recovery from
sewage treatment plant. However, the net energy balance tabulation
shows that the MW pre-treatment leads to negative net energy production.
Abstract: Anogeissus leiocarpus (Combretaceae) is well known
for its medicinal uses in African traditional medicine, for treating
many human diseases mainly skin diseases and infections. Mycetoma
disease is a fungal and/ or bacterial skininfection, mainly cause by
Madurella mycetomatis fungus. This study was carried out in vitro to
investigate the antifungal activity of Anogeissus leiocarpus leaf
extracts against the isolated pathogenic Madurella mycetomatis, by
using the NCCLS modified method compared to Ketoconazole
standard drug, and MTT assay. The bioactive fraction was subjected
to chemical analysis implementing different chromatographic
analytical methods (TLC, HPLC, and LC-MS/MS). The results
showed significance antifungal activity of A. leiocarpus leaf extracts
against the isolated pathogenic M. mycetomatis, compared to negative
and positive controls. The chloroform fraction showed the highest
antifungal activity. The chromatographic analysis of the chloroform
fraction with the highest activity showed the presence of important
bioactive compounds such as ellagic and flavellagic acids derivatives,
flavonoids and stilbenoid, which are well known for their antifungal
activity.
Abstract: As a result of the ambiguity and complexity
surrounding anaerobic digester foaming, efforts have been made by
various researchers to understand the process of anaerobic digester
foaming so as to proffer a solution that can be universally applied
rather than site specific. All attempts ranging from experimental
analysis to comparative review of other process has not fully
explained the conditions and process of foaming in anaerobic
digester. Studying the current available knowledge on foam
formation and relating it to anaerobic digester process and operating
condition, this piece of work presents a succinct and enhanced
understanding of foaming in anaerobic digesters as well as
introducing a simple method to identify the onset of anaerobic
digester foaming based on analysis of historical data from a field
scale system.
Abstract: A field experiment was conducted in the Agricultural
Research Station, at El-Ghoraieb, Assiut to study dry seed yield
performance of two locally adapted cultivars (‘Azmerly’ and ‘Cream
7’) and two line introductions (IT81D-1032 and IT82D-812) of
common cowpea (Vigna unguiculata (L.) Walp) grown at three
different within-row spaces (20, 30 and 40 cm) and two planting
dates in the summer (April 15th and 30th) and in the fall season (Aug.
12th and 27th) of two successive seasons. The data showed that total
dry-seed yield produced by plants grown at 20 cm was greater than at
30 cm in all cvs/lines in both years. Increases in 1000-seed weight
were detected in cv ‘Azmerly’ and line IT82D-812 when they were
grown at 30 cm as compared with 20 cm in the summer season.
However, in the fall season such increases were found in all cvs/lines.
Planting at 40 cm produced seeds of greater weight than planting at
30 cm for all cvs/lines in the fall season and also in cv. Cream 7 and
line IT82D-812 in the summer season, while all cvs/lines in the fall
Planting on April 15th in the summer and also planting on Aug. 12th
in the fall had plants which showed increases in 1000-seed weight
and total dry-seed yield. The greatest 1000-seed weight was found in
the line IT81D-1032 in the summer season and in the line IT82D-812
in the fall season. The sum up results revealed that ‘Azmerly’
produced greater dry-seed yield than ‘Cream 7’ and both of them
were superior to the line IT82D-812 and IT81D-1032 in the summer
season. In the fall, however, the line IT82D-812 produced greater
dry-seed yield than the other cultivars/lines.
Abstract: In order to investigate the prebiotic potential of
oligosaccharides prepared by chemical hydrolysis of water-soluble
polysaccharides (WSP) from Zizyphus lotus leaves, the effect of
oligosaccharides on bacterial growth was studied. The chemical
composition of WSP was evaluated by colorimetric assays revealed
the average values: 7.05±0.73% proteins and 86.21±0.74%
carbohydrates, among them 64.81±0.42% is neutral sugar and the rest
16.25±1.62% is uronic acids. The characterization of
monosaccharides was determined by high performance anion
exchange chromatography with pulsed amperometric detection
(HPAEC-PAD) was found to be composed of galactose (23.95%),
glucose (21.30%), rhamnose (20.28%), arabinose (9.55%), and
glucuronic acid (22.95%). The effects of oligosaccharides on the
growth of lactic acid bacteria were compared with those of fructooligosaccharide
(RP95). The oligosaccharides concentration was
1g/L of Man, Rogosa, Sharpe broth. Bacterial growth was assessed
during 2, 4.5, 6.5, 9, 12, 16 and 24 h by measuring the optical density
of the cultures at 600 nm (OD600) and pH values. During
fermentation, pH in broth cultures decreased from 6.7 to 5.87±0.15.
The enumeration of lactic acid bacteria indicated that
oligosaccharides led to a significant increase in bacteria (P≤0.05)
compared to the control. The fermentative metabolism appeared to be
faster on RP95 than on oligosaccharides from Zizyphus lotus leaves.
Both RP95 and oligosaccharides showed clear prebiotic effects, but
had differences in fermentation kinetics because of to the different
degree of polymerization. This study shows the prebiotic
effectiveness of oligosaccharides, and provides proof for the selection
of leaves of Zizyphus lotus for use as functional food ingredients.