Abstract: This research work is an experimental study, through
development of an adhesive from Prosopis africana endosperm. The
prosopis seed for this work were obtained from Enugu State in the
South East part of Nigeria. The seeds were prepared by separating the
endosperm from the seed coat and cotyledon. Three methods were
used to separate them, which are acidic method, roasting method and
boiling method. 20g of seed were treated with different
concentrations (25, 40, 55, 70, and 85% w/w) at 100°C and constant
time (30 minutes), under continuous stirring with magnetic stirrer.
Also 20g of seed were treated with sulphuric acid of concentrations
40% w/w at 100°C with different time (10, 15, 20, 25, 30 minutes),
under continuous stirring with magnetic stirrer. Finally, 20g of seed
were treated with sulphuric acid of concentrations 40% w/w at
different temperature (20°C, 40°C, 60°C, 80°C, and 100°C) with
constant time (30 minutes), under continuous stirring with magnetic
stirrer. The whole endosperm extracted was adhesive. The physical
properties of the adhesive were determined (appearance, odour, taste,
solubility, pH, size, and binding strength). The percentage of the
adhesive yield makes the commercialization of the seed in Nigeria
possible and profitable. The very high viscosity attained at low
concentrations makes prosopis adhesive an excellent thickener in the
food industry.
Abstract: Wheat is the first and the most important grain of the
world and its bakery property is due to glutenin and gliadin qualities.
Wheat seed proteins were divided into four groups according to
solubility including albumin, globulin, glutenin and prolamin or
gliadin. Gliadins are major components of the storage proteins in
wheat endosperm. It seems that little information is available about
gliadin genes in Iranian wild relatives of wheat. Thus, the aim of this
study was the evaluation of the wheat wild relatives collected from
different origins of Zagros Mountains in Iran, in terms of coding
gliadin genes using specific primers. For this, forty accessions of
Triticum boeoticum and Triticum urartu were selected for this study.
For each accession, genomic DNA was extracted and PCRs were
performed in total volumes of 15 μl. The amplification products were
separated on 1.5% agarose gels. In results, for Gli-2A locus three
allelic variants were detected by Gli-2As primer pairs. The sizes of
PCR products for these alleles were 210, 490 and 700 bp. Only five
(13%) and two accessions (5%) produced 700 and 490 bp fragments
when their DNA was amplified with the Gli.As.2 primer pairs.
However, 93% of the accessions carried allele 210 bp, and only 8%
did not any product for this marker. Therefore, these germplasm
could be used as rich gene pool to broaden the genetic base of bread
wheat.