Abstract: Wheat is the first and the most important grain of the
world and its bakery property is due to glutenin and gliadin qualities.
Wheat seed proteins were divided into four groups according to
solubility including albumin, globulin, glutenin and prolamin or
gliadin. Gliadins are major components of the storage proteins in
wheat endosperm. It seems that little information is available about
gliadin genes in Iranian wild relatives of wheat. Thus, the aim of this
study was the evaluation of the wheat wild relatives collected from
different origins of Zagros Mountains in Iran, in terms of coding
gliadin genes using specific primers. For this, forty accessions of
Triticum boeoticum and Triticum urartu were selected for this study.
For each accession, genomic DNA was extracted and PCRs were
performed in total volumes of 15 μl. The amplification products were
separated on 1.5% agarose gels. In results, for Gli-2A locus three
allelic variants were detected by Gli-2As primer pairs. The sizes of
PCR products for these alleles were 210, 490 and 700 bp. Only five
(13%) and two accessions (5%) produced 700 and 490 bp fragments
when their DNA was amplified with the Gli.As.2 primer pairs.
However, 93% of the accessions carried allele 210 bp, and only 8%
did not any product for this marker. Therefore, these germplasm
could be used as rich gene pool to broaden the genetic base of bread
wheat.
Abstract: In order to study floristic and molecular classification
of common wild wheat (Triticum boeoticum Boiss.), an analysis was
conducted on populations of the Triticum boeoticum collected from
different regions of Iran. Considering all floristic compositions of
habitats, six floristic groups (syntaxa) within the populations were
identified. A high level of variation of T. boeoticum also detected
using SSR markers. Our results showed that molecular method
confirmed the grouping of floristic method. In other word, the results
from our study indicate that floristic classification are still useful,
efficient, and economic tools for characterizing the amount and
distribution of genetic variation in natural populations of T.
boeoticum. Nevertheless, molecular markers appear as useful and
complementary techniques for identification and for evaluation of
genetic diversity in studied populations.