Identification and Classification of Gliadin Genes in Iranian Diploid Wheat

Wheat is the first and the most important grain of the world and its bakery property is due to glutenin and gliadin qualities. Wheat seed proteins were divided into four groups according to solubility including albumin, globulin, glutenin and prolamin or gliadin. Gliadins are major components of the storage proteins in wheat endosperm. It seems that little information is available about gliadin genes in Iranian wild relatives of wheat. Thus, the aim of this study was the evaluation of the wheat wild relatives collected from different origins of Zagros Mountains in Iran, in terms of coding gliadin genes using specific primers. For this, forty accessions of Triticum boeoticum and Triticum urartu were selected for this study. For each accession, genomic DNA was extracted and PCRs were performed in total volumes of 15 μl. The amplification products were separated on 1.5% agarose gels. In results, for Gli-2A locus three allelic variants were detected by Gli-2As primer pairs. The sizes of PCR products for these alleles were 210, 490 and 700 bp. Only five (13%) and two accessions (5%) produced 700 and 490 bp fragments when their DNA was amplified with the Gli.As.2 primer pairs. However, 93% of the accessions carried allele 210 bp, and only 8% did not any product for this marker. Therefore, these germplasm could be used as rich gene pool to broaden the genetic base of bread wheat.

Authors:

• Jafar Ahmadi
• Alireza Pour-Aboughadareh

Keywords:

• Diploied wheat
• gliadin
• Triticum boeoticum
• Triticum urartu.

References:

[1] B. L. Johnson, H. S. Dhaliwal, “Reproductive isolation of Triticum
boeoticum and Triticum urartu and the origin of the tetraploid wheats”
American Journal of Botany, 1976, 65: 907-918.
[2] V. F. Dorofeev, A. A. Filatenko, E. F. Migushova, R.A. Udaczin and M.
M. Jakubziner, “Wheat” 1979, vol. 1. In: V.F. Dorofeev, O.N. Korovina,
(eds.), Flora of Cultivated Plants. Leningrad, Russia. [3] J. Dvorak, “Transfer of leaf rust resistance from Aegilops speltoides to
Triticum aestivum” Canadian Journal of Genetics and Cytology, 1977,
19: 133-141.
[4] B. Kilian, H. Ozkan, O. Deusch, S. Effgen, A. Brandolini, J. Kohl, W.
Martin, F. Salamini, “ Independent wheat B and G genome origins in
outcrossing Aegilops progenitor haplotypes. Molecular Biology and
Evolution, 2007, 24: 217-227.
[5] M. Heun, R. Schafer-Pregl, D. Klawan, R. Castagna, M. Accerbi, B.
Borghi, F. Salamini, “Site of Einkorn Wheat Domestication Identified
by DNA Fingerprinting” Science, 1997, 278: 1312–1314.
[6] P. L. Weegels, R.J. Hamer, J. D. Schofield “Functional properties of
wheat glutenin” Cereal Sci. 1996, 23: 1-17.
[7] P. R. Shewry, N. G. Halford, A. S. Tatham “High molecular weight
subunits of wheat glutenin” J. Cereal Sci. 1992, 15: 105-120.
[8] S. Masci, R. Ovidio, D. Lafiandra, D.D. Kasarda “Characterization of a
low molecular weight glutenin subunit gene from bread wheat and the
corresponding protein that represents a major subunit of the glutenin
polymer” Plant Physiology, 1998, 118: 1147-1158.
[9] K. Kawaura, K., Mochida, Y. Ogihara, “ Expression Profile of Two
Storage-Protein Gene Families in Hexaploid Wheat Revealed by Large-
Scale Analysis of Expressed Sequence Tags” Plant Physiology, 2005,
139:1870-1880.
[10] M.W. Van Slageren, “Wild wheats. A monograph of Aegilops L. and
Amblyopyrum (Jaub. & Spach) Eig (Poaceae)” Wageningen
Agricultural University, 1994, pp. 512.
[11] S. Piccolo, A. Alfonzo, G. Conigliaro, G. Moschetti, S. Burruano, A.
Barone, “A simple and rapid DNA extraction method from leaves of
grapevine suitable for polymerase chain reaction analysis” African
Journal of Biotechnology, 2012, 11: 10305-10309.
[12] H. Long, Y. Wei, Z.H. Yan, B. Baum, E. Nevo, Y.L. Zheng,
“Classification of wheat low-molecular-weight glutenin subunit genes
and its chromosome assignment by developing LMW-GS group-specific
primers” Theoretical and Applied Genetics, 2005, 111: 1251-1259.
[13] K. Kawaura, K. Mochida, Y. Ogihara, “Expression Profile of Two
Storage-Protein Gene Families in Hexaploid Wheat Revealed by Large-
Scale Analysis of Expressed Sequence Tags” Plant Physiology, 2005,
139:1870-1880.
[14] M. Zhao-cai, Q. Chen, Y. Zheng, “Allelic identification and genetic
diversity at Gli-A1 and Gli-A2 loci in Einkorn wheat” International
Journal of Agricultural Research, 2006, 1: 100-107.
[15] L. Wang, G. Li, R.J. Penea, X. Xia and Z. He, “Development of STS
markers and establishment of multiplex PCR for Glu-A3 alleles in
common wheat (Triticum aestivum L.)” Journal of Cereal Science, 2010,
51: 305-312.
[16] S. Sharma, S. Ram, R. Gupta, I. Sharma, “Development of functional
marker for distinguishing Glu-B3b allele of LMW-GS found in Indian
common wheat cultivars” Journal of Cereal Science, 2013, 57: 245-248.
[17] Y. Q. Gu, C. Crossman, X. Kong, M. Luo, F.M. You, D. Coleman-Derr,
J. Dubcovsky, O.D. Anderson, “Genomic organization of the complex
alpha-gliadin gene loci in wheat” Theoretical and Applied Genetics,
2004, 109: 648-657.