Effects of Four Dietary Oils on Cholesterol and Fatty Acid Composition of Egg Yolk in Layers

Dietary cholesterol has elicited the most public interest as it relates with coronary heart disease. Thus, humans have been paying more attention to health, thereby reducing consumption of cholesterol enriched food. Egg is considered as one of the major sources of human dietary cholesterol. However, an alternative way to reduce the potential cholesterolemic effect of eggs is to modify the fatty acid composition of the yolk. The effect of palm oil (PO), soybean oil (SO), sesame seed oil (SSO) and fish oil (FO) supplementation in the diets of layers on egg yolk fatty acid, cholesterol, egg production and egg quality parameters were evaluated in a 42-day feeding trial. One hundred and five Isa Brown laying hens of 34 weeks of age were randomly distributed into seven groups of five replicates and three birds per replicate in a completely randomized design. Seven corn-soybean basal diets (BD) were formulated: BD+No oil (T1), BD+1.5% PO (T2), BD+1.5% SO (T3), BD+1.5% SSO (T4), BD+1.5% FO (T5), BD+0.75% SO+0.75% FO (T6) and BD+0.75% SSO+0.75% FO (T7). Five eggs were randomly sampled at day 42 from each replicate to assay for the cholesterol, fatty acid profile of egg yolk and egg quality assessment. Results showed that there were no significant (P>0.05) differences observed in production performance, egg cholesterol and egg quality parameters except for yolk height, albumen height, yolk index, egg shape index, haugh unit, and yolk colour. There were no significant differences (P>0.05) observed in total cholesterol, high density lipoprotein and low density lipoprotein levels of egg yolk across the treatments. However, diets had effect (P<0.05) on TAG (triacylglycerol) and VLDL (very low density lipoprotein) of the egg yolk. The highest TAG (603.78 mg/dl) and VLDL values (120.76 mg/dl) were recorded in eggs of hens on T4 (1.5% sesame seed oil) and was similar to those on T3 (1.5% soybean oil), T5 (1.5% fish oil) and T6 (0.75% soybean oil + 0.75% fish oil). However, results revealed a significant (P<0.05) variations on eggs’ summation of polyunsaturated fatty acid (PUFA). In conclusion, it is suggested that dietary oils could be included in layers’ diets to produce designer eggs low in cholesterol and high in PUFA especially omega-3 fatty acids.

Authors:

• A. F. Agboola
• B. R. O. Omidiwura
• A. Oyeyemi
• E. A. Iyayi
• A. S. Adelani

Keywords:

• Dietary oils
• Egg cholesterol
• Egg fatty acid profile
• Egg quality parameters.

References:

[1] M. R. Mazalli, D. E. Faria, D. Salvador, and D. T. Ito, A comparison of
the feeding value of different sources of fats for laying hens: 1.
Performance characteristics, J. Appl. Poult. Res., 13: 274–279, 2004.
[2] A. Laca, B. Pardes, and M. Diaz, A method of egg yolk fractionation
characterisation of fractions. Food Hydrocolloid, 24: 434-443, 2010.
[3] X. Liu, H. L. Zhao, S. Thiessen, J. D. House, and P. J. H. Jones, Effect
of plant sterol-enriched diets on plasma and egg yolk cholesterol
concentrations and cholesterol metabolism in laying hens. Poult. Sci.,
89: 270-175, 2010.
[4] C. O, Leskanish, and R. C. Noble, Manipulation of the n-3
polyunsaturated fatty acid composition of avian meat. World’s Poult.
Sci. J., 53: 156-182, 1997.
[5] S.E. Chowdhury, S. D. Chowdhury, and T. K. Smith, Effects of dietary
garlic on cholesterol metabolism in laying hens. Poult. Sci., 81: 1856-
1862, 2002.
[6] L. Vorlava, E. Sieglova, R. Karpiskova, and V. Kopriva, Cholesterol
content in eggs during the laying period. Acta Veterinaria Brno. 70: 387-
390, 2001.
[7] R. G. Elkin, Reducing shell egg cholesterol content. I. Overview, genetic
approaches, and nutritional strategies. World’s Poult. Sci. J., 62: 665-
687, 2006.
[8] I. K. Paik, and R. Blair, Atherosclerosis, Cholesterol and Eggs. AJAS. 9
(1): 1-25, 1995.
[9] A. C. Beynen, Fatty acid composition of eggs produced by hens fed diets
containing groundnut, soybean or linseed. N. J. Anim. Sci., 52, 3– 10,
2004.
[10] J. G. Souza, F. G. P. Costa, R. C. R. E. Queiroga, S. H. V. Silva, A. R. P.
Schwler, and C. C. Goulart, Fatty acid profile of eggs of semi-heavy
layers fed feeds containing linseed oil. Braz. J. Poultry Sci., 10: 37-44,
2008.
[11] Food Standards Agency (FSA). McCance and Widdowson’s: The
composition of Foods (Sixt Summary Edition). Royal Society of
Chemistry, Cambridge. 2002.
[12] R. S. Filardi, O. M. Junqueira, A. C. DE Laurentiz, E. M. Casartelli, E.
A. Rodriques, and L. F. Araujo, Influence of different fat sources on the
performance, egg quality, and lipid profile of egg yolks of commercial
layers in the second laying cycle. J. Appl. Poultry Res., 14: 258-264,
2005.
[13] A. P. Simopoulos, A. P. The omega-6/omega-3 ratio: The scientific
evidence and the need to return omega-3 fatty acids into eggs and other
food. In: (ed). Sim, J. and Sinwoo, H. H. The Amazing Egg: Nature’s
Perfect Functional Food for Health Promotion. University of Atwertal
Edmonton, Alberta, Canada, 2006.
[14] H. Basmacioglu, M. Cabuk, K. Unal, K., Ozkan, S. Akkan, and H.
Yalcin, Effects of diatary fish oil and flax seed on cholesterol and fatty
acid composition of egg yolk and blood parameters of laying hens. S.
Afr. J. Anim. Sci. 33: 4, 2003.
[15] M. J. Gonzalez-Munoz, S. Bastida, O. Jimenez, Lorenzo de C., G.
Vergara, and F. J. Sachez-Muniz, The effect of dietary fat on the fatty
acid composition and cholesterol content of eggs from Hy-line and
Warren hens Grassas Y Aceites. 60(4): 350-359, 2009.
[16] J. B. Lawlow, N. Gaudette, T. Dickson, and J. D. House, Fatty acid
profile and sensory characteristics of table eggs from laying hens fed
diets containing microencapsulated fish oil. J. Anim. Feed Sci. and
Tech., 156: 97-103, 2010.
[17] N. Ceylan, I. Ciftci, C. Mizrak, Z. Kahraman, and H. Efil. (2011).
Influence of different dietary oil sources on performance and fatty acid
profile of egg yolk in laying hens. J. Anim. Feed Sci.,, 20: 71-83, 2011.
[18] NRC (National Research Council). Nutrient Requirements of Poultry.
National Academy Press: Washington. DC. 9th ed. Pp. 35-39, 1994.
[19] P. C. Panda, Shape and Texture. In: Textbook on egg and poultry
technology (First Edition). New Delhi, India, 57, 1996.
[20] S. D. Chowdhury, Effects of feeding steolathyrogens to laying fowl
(Gallus domestics) with particular reference to eggshell and membrane
quality. Ph.D. Thesis, University of London, UK, 1987.
[21] G. Doyon, M. Bernier-Cardou, R. M. G. Hamilton, F. Eastaigns, C. T.
and Ramdald, Egg quality: Albumen quality of egg from five
commercial strains of White Leghorn hens during one year of lay. Poult.
Sci., 65: 63-66. 1986.
[22] R. R. Haugh, The Haugh unit for measuring egg quality. US Egg Poult.
Mag., 43: 522-555, 1937.
[23] AOAC, Official and Tentative Methods of the American Oil Chemist’
Society, 3rd edition, champaign, Illinois, 1978.
[24] R. G. Elkin, Z. Yan, Y. Zhong, S. S. Donkin, K. K. Burham, J. A. Story,
J. J. Turek, R. E. Porter, M. Anderson, R. Homan, and R. S. Newton,
Select 3-Hydroxyl-3-Methylglutaryl-coenzyme: a reductase inhibitors
vary in their ability to reduce egg yolk cholesterol levels in varying
alternation of hepatic cholesterol synthesis and plasma VLDL
composition. J. Nutr., 129: 1010-1019, 1999.
[25] W. T. Friedewald, R. I. Levy, and D. S, Fredrickson, Estimation of the
concentration of low-density lipoprotein cholesterol in plasma, without
use of the preparative ultracentifuge. Clin. Chem., 18 (6): 499-502,
1972.
[26] AOAC, Association of official analytical chemists. Official methods of
analysis 16th Ed., Published by Association of Official Analytical
Chemists. Inc., Washington, D. C., USA, 2000.
[27] SAS, Statistical Analysis Systems User’s guide (12.1 Ed.). SAS Institute
Inc., Raleigh North Carolina, 2012.
[28] D. B. Duncan, Multiple range and multiple F tests. Biometrics, 11: 1-42,
1955.
[29] A. Meluzzi, F. Sirri, G. Manfreda, N. Tallarico, and A. Franchini,
Effects of dietary vitamin E on the quality of table eggs enriched with n-
3 long chain fatty acids. Poult. Sci., 79: 539– 545, 2000.
[30] M. D. Baucells, N. Crespo, A. C. Barroeta, S. Lopez-Ferrer, and M. A.
Grashorn, Incorporation of different polyunsaturated fatty acids into
eggs. Poult. Sci., 79: 51– 59, 2000.
[31] M. C. G. Pita, E. Piber Neto, P. R. Carvalho, and C. X. Mendonça
Junior, Efeito da suplementação de linhaça, óleo de canola e vitamina E
na dieta sobre as concentrações de ácidos graxos poliinsaturados em
ovos de galinha. Arq. Bras. Méd. Vet. Zootec. 58: 925–931, 2006.
[32] T. Ebeid, Y. Eid, A. Saleh, and H. Abd El-Hamid, Ovarian follicular
development, lipid peroxidation, antioxidative status, and immune
response in laying hens fed fish oil-supplemented diets to produce n-3-
enriched eggs. Animal, 2: 84– 91, 2008.
[33] E. Atakisi, O. Atakisi, H. Yaman, and I. Arslan, I. Omega-3 fatty acid
application reduces yolk and plasma cholesterol levels in Japanese
quails. Food Chem. Toxicol. 47: 2590-2593, 2009.
[34] M. C. Milinsk, A. E. Murakami, S. T. M. Gomes, M. Matsushita, and N.
E. De Souza, Fatty acid profile of egg yolk lipids from hens fed diets
rich in n-3 fatty acids. Food Chem. 83: 287-292, 2003.
[35] N. A. Botsoglou, L. A. Yannakopoulos, D. J. Fletouris, A. S. Tserveni-
Goussi, and I. E. Psomas,. Yolk fatty acid composition and cholesterol
contents in response to level and form of dietary linseed. J. Agri. Food
Chem., 46: 4652– 4656, 1998.
[36] S. Carrillo-Dominguez, I. M. E. Carranco-Jauregui, R. M. Castillo-
Domynguez, and M. I. Castro-Gonzalez, Cholesterol and n-3 and n-6
fatty acid content in eggs from laying hens fed with red crab meal
(Pleuroncodes planipes). Poult. Sci., 84: 167– 172, 2005.
[37] M. Schreiner, H. W. Hulan, E. Razzazi-Fazeli, J. Bohm, and C. Iben,
Feeding laying hens seal blubber oil: Effects on egg yolk incorporation,
stereospecific distribution of omega-3 fatty acids, and sensory aspects.
Poult. Sci., 83: 462– 473, 2004.
[38] G. J. M. Coetzee, and L. C. Hoffman, Effects of various dietary n-3/n-6
fatty acid ratios on the performance and body composition of broilers. S.
Afr. J. Anim. Sci., 32(3): 175– 183, 2002.
[39] P. S. Hargis, M. E. Van Elswyk, and B. M. Hargis, Dietary Modification
of Yolk Lipid with Menhaden Oil. Poult. Sci., 70 (4): 874-883, 1991.
[40] I. D. Bean, and S Leeson, Long-term effect of feeding linseed on
performance and egg fatty acid composition of brown and white hens.
Poult. Sci., 82: 388– 394, 2003.
[41] R. Augustyn, J. Barteczko, and S. Smulikowska, The effect of feeding
regular or low α-linolenic acid linseed on laying performance and total
cholesterol content in eggs. J. Feed Sci., 15: 103–106 (Suppl. 1), 2006.
[42] E. Sosin, F. Borowiec, J. Strzetelski, and S. mulikowska, The effect of
feeding regular or low α-linolenic acid linseed on the fatty acid
composition of egg yolks. J. Anim. Feed Sci., 15: 641– 650, 2006.
[43] E. Rowghani, M. Arab, S. Nazifi, and Z. Bakhtiari, Effect of canola oil
on cholesterol and fatty acid composition of egg-yolk of laying hens. Int.
J. Poult. Sci., 6(2): 111–114, 2007.
[44] C. Novak, and S. E. Scheideler, Long-term effects of feeding linseedbased
diets. 1. Egg production parameters, components, and eggshell
quality in two strains of laying hens. Poult. Sci., 80: 1480– 1489, 2001. [45] Karunajeewa, H., Than, S. H. and Abu-Serewa, S. (1989). Sunflower
seed meal, sunflower oil and full-fat sunflower seeds, hulls and kernels
for laying hens. Anim. Feed Sci. Technol., 26: 45-54.
[46] Y. H. Jiang, R. B. McGeachin, and C. A. Bailey, α-tocopherol, β-
carotene, and retinol enrichment of chicken eggs. Poult. Sci., 73:1137–
1143, 1994.
[47] Scheideler, S. E., Jaroni, D. and Froning, G. (1998). Strain and age
effects on egg composition from hens fed diets rich in n-3 fatty acids.
Poult. Sci., 77: 192–196.