The Key Role of the Steroidal Hormones in the Pattern Distribution of the Epiphyseal Structure in Rabbit

Steroidal hormones with the efficient changes on the epiphyseal growth plate may influence tissue structure properties. Presents paper to investigate the effects of gonadectomy in the pattern distribution of the epiphyseal structure. Fifteen adult female New Zealand white rabbits were separated into three groups. One group was intact and others groups were selected for surgical operation. From these two groups, one group carried out steroidal administration. The results obtained showed that there is no statistically difference in the mean diameter of the growth plate cells between all three groups. The maximum value of the cartilage cells were allocated to the gonadectomized group and the minimum number were observed in Hormonal induced group significantly. Growth plate height was significantly greater in gonadectomized group than in two other groups.

Detection of Pathogenic Escherichia coli Strains Pollution in Red Deer Meat in Latvia and Determination the Compatibility of VT1, VT2, eae A Genes in their Isolate

Tasks of the work were study the possible E.coli contamination in red deer meat, identify pathogenic strains from isolated E.coli, determine their incidence in red deer meat and determine the presence of VT1, VT2 and eaeA genes for the pathogenic E.coli. 8 (10%) samples were randomly selected from 80 analysed isolates of E.coli and PCR reaction was performed on them. PCR was done both on initial materials – samples of red deer meat - and for already isolated liqueurs. Two of analysed venison samples contain verotoxin-producing strains of E. coli. It means that this meat is not safe to consumer. It was proven by the sequestration reaction of E. coli and by comparison of the obtained results with the database of microorganism genome available on the internet that the isolated culture corresponds to region 16S rDNS of E. coli thus presenting correctness of the microbiological methods.

Animal-Assisted Therapy for Persons with Disabilities Based on Canine Tail Language Interpretation via Gaussian-Trapezoidal Fuzzy Emotional Behavior Model

In order to alleviate the mental and physical problems of persons with disabilities, animal-assisted therapy (AAT) is one of the possible modalities that employs the merit of the human-animal interaction. Nevertheless, to achieve the purpose of AAT for persons with severe disabilities (e.g. spinal cord injury, stroke, and amyotrophic lateral sclerosis), real-time animal language interpretation is desirable. Since canine behaviors can be visually notable from its tail, this paper proposes the automatic real-time interpretation of canine tail language for human-canine interaction in the case of persons with severe disabilities. Canine tail language is captured via two 3-axis accelerometers. Directions and frequencies are selected as our features of interests. The novel fuzzy rules based on Gaussian-Trapezoidal model and center of gravity (COG)-based defuzzification method are proposed in order to interpret the features into four canine emotional behaviors, i.e., agitate, happy, scare and neutral as well as its blended emotional behaviors. The emotional behavior model is performed in the simulated dog and has also been evaluated in the real dog with the perfect recognition rate.

Preparation Influences of Breed, sex and Sodium Butyrate Supplementation on the Performance, Carcass Traits and Mortality of Fattening Rabbits

Twenty four New Zealand white rabbits (12 does and 12 bucks) and twenty four Flanders (12 does and 12 bucks) rabbits, allotted into two feeding regime (6 for each breed, 3 males and 3 females) first one fed commercial ration and second one fed commercial diet plus sodium butyrate (300 g/ton). The obtained results showed that at end of 8th week experimental period New Zealand white rabbits were heavier body weight than Flanders rabbits (1934.55+39.05 vs. 1802.5+30.99 g); significantly high body weight gain during experimental period especially during 8th week (136.1+3.5 vs. 126.8+1.8 g/week); better feed conversion ratio during all weeks of experiment from first week (3.07+0.16 vs. 3.12+0.10) till the 8th week of experiment (5.54+0.16 vs. 5.76+0.07) with significantly high dressing percentages (0.54+0.01 vs. 0.52+0.01). Also all carcass cuts were significantly high in New Zealand white rabbits than Flanders. Females rabbits (at the same age) were lower body weight than males from start of experiment (941.1+39.8 vs.972.1+33.5 g) till the end of experiment (1833.64+37.69 vs. 1903.41+36.93 g); gained less during all weeks of experiment except during 8th week (132.1+2.3 vs. 130.9+3.4 g/week), with lower dressing percentage (0.52+0.01 vs. 0.53+0.01) and lighter carcass cuts than males, however, they had better feed conversion ratio during 1st week, 7th week and 8th week of experiment. Addition of 300g sodium butyrate/ton of rabbit increased the body weight of rabbits at the end of experimental period (1882.71+26.45 vs. 1851.5+49.82 g); improve body weight gain at 3rd, 4th, 5th, 6th and 7th week of experiment and significantly improve feed conversion ratio during all weeks of the experiment from 1st week (2.85+0.07 vs. 3.30+0.15) till the 8th week of the experiment (5.51+0.12 vs. 5.77+0.12). Also the dressing percentage was higher in Sodium butyrate fed groups than control one (0.53+0.01 vs. 0.52+0.01) and the most important results of feeding sodium butyrate is the reducing of the mortality percentage in rabbits during 8 week experiment to zero percentage as compared with 16% in control group.

Fatty Acids Composition of Elk, Deer, Roe Deer and Wild Boar Meat Hunted in Latvia

A game animals – elk (Alces alces), deer (Cervus elaphus), roe deer (Capreolus capreolus) or wild boar (Sus scrofa scrofa) - every autumn and winter period provide an excellent investment, diversification of many consumer meals. In last years consumption and assortiment of game meat products significantly increase. Investigations about biochemical composition of game meat are not very much. The meat of wild animals is more favourable for human health because it has lower saturated fatty acids content, but higher content of protein. Therefore the aim of investigations was to compare biochemical composition of ungulates obtained in Latvia.Investigations were carried out in wild animals different regions of Latvia. In the studied samples protein, intramuscular fat, fatty acids and cholesterol were determined. The biochemical analysis of 54 samples were done. Results of analysis showed that protein content 22.36 – 22.92% of all types of meat samples is not different statistically, significantly lower fat content 1.33 ± 0.88% had elk meat samples and 1.59 ± 0.59% roe deer samples. Content of cholesterol was various 64.41 – 95.07% in the ruminant meat samples of different species. From the dietetic point of view the best composition of fatty acids has meat samples of roe deer.

Effect of Dietary Supplementation of Different Levels of Black Seed (Nigella Sativa L.) on Growth Performance, Immunological, Hematological and Carcass Parameters of Broiler Chicks

This experiment was conducted to investigate the effect of dietary supplementation of different levels of black seed (Nigella sativa L.) on the performance and immune response of broiler chicks. A total 240 day-old broiler chicks were used and randomly allotted equally into six experimental groups designated as 1, 2, 3, 4, 5 and 6 having black seed at the rate of 0, 2, 4, 6, 8 and 10 g /kg diet respectively. The study was lasted for 42 days. Average body weight, weight gain, relative growth rate, feed conversion, antibody titer against Newcastle disease, phagocytic activity and phagocytic index, some blood parameters(GOT, GPT, Glucose, Cholesterol, Triglyceride, Total protein, Albumen, WBCs, RBCs, Hb and PCV), dressing percentage, weight of different body organs, abdominal fat weight, were determined. It was found that, N. Sativa significantly improved final body weight, total body gain and feed conversion ratio of groups 2 and 3 when compared with the control group. Higher levels of N. Sativa did not improve growth performance of the chicks. Non significant differences were observed for antibody titer against Newcastle virus, WBCs count, serum GOT, glucose level, dressing %, relative liver, spleen, heart and head percentages. Lymphoid organs (Bursa and Thymus) improved significantly with increasing N. Sativa level in all supplemented groups. Serum cholesterol, triglyceride and visible fat % significantly decreased with Nigella sativa supplementation while serum GPT level significantly increased with nigella sativa supplementation.

The Effects of Feeding Raw Fiber Concentrate on Growth Performance and Blood Metabolites of Suckling Holstein Calves

Sixteen female Holstein calves allocated in three treatments including: 1: control, 2: fed raw fiber concentrate (RFC) for 45 days and 3: fed RFC for 90 days. RFC supplement (Vitacel® 200) was added to milk immediately before feeding (10 g/L milk). Withers height and body weights of calves were measured monthly. Individual dry matter intake was recorded daily. Blood samples were taken monthly. The result showed that calves consumed RFC had significantly greater weaning and final body weight. Treatment effect on dry matter intake was not significant (p>0.05). Calves fed RFC had better feed efficiency. Withers height of calves fed RFC were taller than the control group (p

The Relationship between Sheep Management and Lamb Mortality

This study was carried out to investigate lamb mortalities relating to ewes' breed and some managemental factors on 250 pregnant ewes (190-Rahmani, 30-Ossimi and 30-Romanov) at Mehallet Mousa, Animal Production Research Station, Kafr El- Sheikh Province, Egypt. These animals divided into five groups according to the managemental factors used. The results revealed that the lamb mortality was higher in Ossimi breed and lower in Romanov one. In addition, the highest lamb mortality occurred among lambs for unsupplemented ewes, for those had body condition score two and for lambs which born outdoor. Moreover, the lamb survivability was increased by the parity of ewes. From this study it can be concluded that the lamb mortality depends on ewes' body condition score, parity, lambing system (indoor or outdoor), nutrition during pregnancy period and selected breed. In addition, the most important period for lamb survival is the first week of age.

Ultrasonic Evaluation of Bone Callus Growth in a Rabbit Tibial Distraction Model

Ultrasound is useful in demonstrating bone mineral density of regenerating osseous tissue as well as structural alterations. A proposed ultrasound method, which included ultrasonography and acoustic parameters measurement, was employed to evaluate its efficacy in monitoring the bone callus changes in a rabbit tibial distraction osteogenesis (DO) model. The findings demonstrated that ultrasonographic images depicted characteristic changes of the bone callus, typical of histology findings, during the distraction phase. Follow-up acoustic parameters measurement of the bone callus, including speed of sound, reflection and attenuation, showed significant linear changes over time during the distraction phase. The acoustic parameters obtained during the distraction phase also showed moderate to strong correlation with consolidated bone callus density and micro-architecture measured by micro-computed tomography at the end of the consolidation phase. The results support the preferred use of ultrasound imaging in the early monitoring of bone callus changes during DO treatment.

Sperm Production Rate, Gonadal and Extragonadal Sperm Reserves in the Sokoto Red (Maradi) Buck in a Tropical Environment

28 healthy adult Maradi bucks were used to evaluate sperm production and sperm storage capacity in the breed. Daily sperm production (DSP) averaged 0.55±0.05x109, while the daily sperm production/g (DSP/g) was 1.37±0.12 x107. Gonadal sperm reserve was 1.99±0.18 x109, while the caput, upper corpus and lower corpus averaged 0.58±0.04 x109, 0.36±0.02 x109 and 0.33±0.08 x109 respectively. The proximal cauda, mid cauda, distal cauda and ductus deferens had values of 0.68±0.10 x109, 1.23±0.16 x109,1.87±0. x109and 0.17±0.05 x109 respectively. The relative contributions of the respective epididymal sections and ductus deferens to the total extragonadal sperm reserves were, 11.11%, 6.89%, 6.32%, 13.03%, 23.56%, 35.82% and 3.26% respectively. Gonadal sperm reserves were significantly higher (p0.05) to mid cauda and distal cauda epididymal reserves.

Muscularity and Leg Tissue Composition of Lambs Fed with Hydrolyzed Sugarcane

This study aimed to evaluate the muscularity and tissue composition of 24 legs of Ile de France lambs. They were fed with diets containing “in nature" or hydrolyzed sugarcane with 0.6% of calcium oxide in aerobic and anaerobic environments. Animals entered the trial at 15 and were slaughtered at 32 kg of body weight. The leg tissue composition, as well as muscularity (0.47), muscle:bone (6.66) and muscle:fat (4.25) were not affected (P>0.05) by treatments. The proportions found were: 67.62% for muscle, 17.52% for bone and 10.15% for fat. In relation to lambs fed with “in nature" sugarcane, hydrolyzed sugarcane with calcium oxide in aerobic and anaerobic environments did not affect muscularity and leg tissue composition of lambs.

Effect of L-Arginine on Neuromuscular Transmission of the Chick Biventer Cervicis Muscle

In this study, the effect of L-arginine was examined at the neuromuscular junction of the chick biventer cervicis muscle. LArginine at 500 μg/ ml, decreased twitch response to electerical stimulation, and produced rightward shift of the dose- response curve for acetylcholine or carbachol. L-Arginine at 1000μg/ ml produced a strong shift to the right of the dose – response curve for acetylcholine or carbachol with a reduction in the efficacy. The inhibitory effect of L-arginine on the twitch response was blocked by caffeine (200μg/ ml). NO levels were also measured in the chick biventer cervicis muscle homogenates, using spectrophotometric method for the direct detection of NO, nitrite and nitrate. Total nitrite (nitrite + nitrate) was measured by a spectrophotometer at 540 nm after the conversion of nitrate to nitrite by copperized cadmium granules. NO levels were found to be significantly increased in concentrations 500 and 1000μg/ ml of L-arginine in comparison with the control group (p

Comparison of the Effects of Three Different Types of Probiotics on the Sucrase Activities of the Small Intestine Mucosa of Broiler Chicks

An experiment was conducted to study the effects of different types of probiotic on Sucrase enzyme activity of the small intestine mucosa in male broilers. The experimental design was arranged as randomized completely blocks in 4 × 2 factorial arrangement of treatment. 180 male broilers of Ross 308 commercial hybrid were designated into 4 groups. Three replicates of 15 birds were assigned to each treatment. Control treatments (diet contained no probiotic) were fed according to the NRC as base diet and three treatment groups were fed from the same diet plus three different types of probiotics. Birds were slaughtered after 21 and 42 days and different segments of small intestine (at 1,10,30,50,70 and 90% of total length the small intestine) were taken from each replicates (N=2) Sucrase enzyme activities were measured and recorded. Obtained data were analyzed by Spss (P

Effect of Castration on CLA in Meat Goats

Twenty four male Thai native × Anglo-Nubian crossbred goats were randomly allocated to receive four treatments. The experiment was conducted for four months and slaughtered that the Longissimus dorsi muscle was collected for fatty acid analysis. The results conclude that either castrated method or ages had no significantly different on monounsaturated fatty acid (MUFA) (P>0.05) except erucic acid (C22:1n9). Interaction between castrated method and ages had significantly different in MUFA (P

Influence of Supplemental Glutamine on Nutrient Digestibility and Utilization, Small Intestinal Morphology and Gastrointestinal Tract and Immune Organ Developments of Broiler Chickens

This study was conducted to investigate the optimum levels of glutamine (Gln) supplementation in broiler diets. A total of 32 one-day-old male chicks with initial body weight 41.5 g were segregated into 4 groups (8 chicks per group) and subsequently distributed to individual cages. Feed and water were provided ad libitum for 21 days. Four dietary treatments were as follows: control and supplemented Gln at 1, 2 and 3%, respectively. The results found that the addition Gln had no negative effects on dry matter, organic matter, ash digestibility or nitrogen retention. Birds fed with 1% Gln had significantly higher villi wide and villi height : crypt depth ratio in duodenum than the control chicks and 2 and 3% Gln chicks. It is suggested that the addition of Gln at 1% indicated a beneficial effect on improving small intestinal morphology, in addition Gln may stimulate immune organ development of broiler chickens.

Minimizing Fish-feed Loss due to Sea Currents: An Economic Methodology

Fish-feed is a major cost component of operating expenses for any aquaculture farm. Due to soaring prices of fish-feed ingredients, the need for better feeding schedule management has become imperative. On such factor that influences the utilization rate of fish-feed are sea currents. Up to now, practical monitoring of fishfeed loss due to sea currents is not exercised. This paper gives a description of an economic methodology that aims at quantifying the amount of fish-feed lost due to sea currents and draws on data from a Mediterranean aquaculture farm to formulate the associated model.

Morpho-histological Study of the Bursa of Fabricius of Broiler Chickens during Post-hashing Age

The study of morphometric and histologic evolutions of the Bursa of Fabricus during 27 weeks of post-hashing age, realized on 88 subjects of broiler chicken they permitted to collect information about the morpho-histological aspect according to their post-hashing age; showed the size and the weight of the Bursa of Fabricius which reach their maximum between the 10th and the 11th week of age and the physiologic involution phenomena. These variations are in close relationship to the sexual maturity. These results can be used in the diagnosis of viral disease such as the Gumboro disease, Marek disease.

Supplementation of Saccharomyces Cerevisiae or Lactobacillus Acidophilus in Goats Diets

This experiment was performed with the purpose of investigating effect of additional blend of probiotics Saccharomyces cerevisiae and Lactobacillus acidophilus on plasma fatty acid profiles particularly conjugated linoleic acid (CLA) in growing goats fed corn silage, and selected the optimal levels of the probiotics for further study. Twenty-four growing crossbred (Thai native x Anglo-Nubian) goats that weighed (14.2 ± 2.3) kg, aged about 6 months, were purchased and allocated to 4 treatments according to Randomized Complete Block Design (RCBD) with 6 goats in each treatment. The blocks were made by weight into heavy, medium, and light goats and each of the treatments contained two goats from each of the blocks. In the mean time, ruminal average pH unaffected, but the NH3-N and also plasma urea nitrogen (p0.05) were raised, but propionic proportion (p0.05) were reduced in concurrent with raise of acetic proportion and resultantly C2:C3 ratio (p>0.05). On plasma fatty acid profiles, total saturated fatty acids (p>0.05) was increased, and contrasted with decrease of C15:0 (p0.05), and C18-C22 polyunsaturated fatty acids (p

Effect of Rearing Systems on Fatty Acid Composition and Cholesterol Content of Thai Indigenous Chicken Meat

The experiment was conducted to study the effect of rearing systems on fatty acid composition and cholesterol content of Thai indigenous chicken meat. Three hundred and sixty chicks were allocated to 2 different rearing systems: conventional, housing in an indoor pen (5 birds/m2); free-range, housing in an indoor pen (5 birds/m2) with access to a grass paddock (1 bird/m2) from 8 wk of age until slaughter. All birds were provided with the same diet during the experimental period. At 16 wk of age, 24 birds per group were slaughtered to evaluate the fatty acid composition and cholesterol content of breast and thigh meat. The results showed that the proportion of SFA, MUFA and PUFA in breast and thigh meat were not different among groups (P>0.05). However, the proportion of n-3 fatty acids was higher and the ratio of n-6 to n-3 fatty acids was lower in free-range system than in conventional system (P0.05). The data indicated that the free-range system could increase the proportion of n-3 fatty acids, but no effect on cholesterol content in Thai indigenous chicken meat.

The Effect of Goat Milk Fractions Supplementation on Serum IgE Response and Leukocytes Count in Dinitrochlorobenzene Sensitized Rat

In Indonesia, goat milk is often consumed and believed as anti-allergy. The objective of this research was to study the effect of goat milk and their fractions (casein and whey) supplementation on total serum IgE concentrations and leukocytes count in rat sensitized with contact allergen dinitrochlorobenzene (DNCB). Female Wistar rats 6-8 weeks old were divided into four groups: 1) whey, 2) casein, 3) whole milk supplementation and 4) phosphate-buffered saline/PBS (control). The results showed that supplementation of goat milk on rats did not affects on total serum IgE concentrations and number of leukocytes. After sensitized with DNCB, the monocyte percentage in rats was higher (P