The Effect of Goat Milk Fractions Supplementation on Serum IgE Response and Leukocytes Count in Dinitrochlorobenzene Sensitized Rat
In Indonesia, goat milk is often consumed and
believed as anti-allergy. The objective of this research was to study
the effect of goat milk and their fractions (casein and whey)
supplementation on total serum IgE concentrations and leukocytes
count in rat sensitized with contact allergen dinitrochlorobenzene
(DNCB). Female Wistar rats 6-8 weeks old were divided into four
groups: 1) whey, 2) casein, 3) whole milk supplementation and 4)
phosphate-buffered saline/PBS (control). The results showed that
supplementation of goat milk on rats did not affects on total serum
IgE concentrations and number of leukocytes. After sensitized with
DNCB, the monocyte percentage in rats was higher (P<0.01) than
before. In conclusion, goat milk or their fractions supplementation
unable to decrease the total serum IgE concentrations and also had
no effect on leukocytes count. However, 1% DNCB could increase
the number of monocytes, but could not induce the IgE response.
[1] J-S. Ham, J-H. Shin, J. Aera, S-G. Jeong, K-W. Park, H-U. Kim, S-Y.
Kang, H. J. Hwang, and W.K. Lee. "Effects of goat milk yogurt
supplemented with citrus concentrate on blood glucose and serum lipids
in diabetic rats". Korean J. Food Sci., vol. 28, no. 4, pp. 445-450, 2008.
[2] G.F. W. Haenlein. "Goat milk in human nutrition". Small Ruminant
Research, vol. 51, pp. 155-163, 2004.
[3] E.I. El-Agamy. The challenge of cow milk allergy. Small Ruminant
Research, vol. 68, pp. 64-72, 2007.
[4] C. Prosser, K. Stelwagen, R. Cummins, P. Guerin, N. Gill, and C.
Milne. "Reduction in heat-induced gastrointestinal hypermeability in
rats by bovine colostrum and goat milk powder". Journal of Applied
Physiology, vol. 96, pp. 650-654, 2003.
[5] F.Y. Wu, P.H. Tsao, D.C. Wang, S. Lin, J.S. Wu, and Y.K. Cheng.
"Factors affecting growth factor activity in goat milk", Journal of Dairy
Science 89: 1951-1955, 2006.
[6] A. Ghaffar. "Hypersensitivity reactions".
http://pathmicro.med.sc.edu/pdfimn2002/02Hypersensitivity.pdf, 2002
[7] H-O. Ku, S-H. Jeong, H-G, Kang, H-M. Pyo, J-H. Cho, S-W. Son, SM.
Yun, and D-Y. Ryu. "Gene expression profiles and pathways in skin
inflammation induced by three different sensitizers and an irritant".
Toxycology Letters, vol. 190, pp. 231-237, 2009
[8] M. Ban and D. Hettich. "Relationship between IgE possitive cell
numbers and serum total IgE levels in mice treated with trimellitic
anhydride and dinitrochlorobenzene". Toxicology letters, vol .118, pp.
129-137, 2001.
[9] C.F. Kuper, R.H. Stierum, A. Boorsma, M.A. Schij, M. Prinsen, J.P.
Bruijntjes, N. Bloksma, J.H.E. Arts. "The contact allergen
dinitrochlorobenzene (DNCB) and respiratory allergy in the Th2-prone
Brown Norway rat".Toxicology, vol. 246, pp. 213-221, 2008.
[10] O.B. Lim, K. Yamada, M. Nonaka, Y. Kuramoto, P. Hung, and M.
Sugano. "Dietary fiber modulate indices of intestinal immune function
in rats", The Journal of Nutrition, vol. 127, no.5, pp. 663-667, 1997.
[11] F.I. Aboderin and V.O. Oyetaya. "Haematological studies of rats fed
different doses of probiotic Lactobacillus plantarum, isolated from
fermenting corn slurry". Pakistan Journal of Nutrition, vol .5 , no. 2, pp.
102-105, 2006.
[12] P.G. Reeves, F.H. Neilsen, and G.C. Fahey, JR. "AIN-93 Purified Diets
for Laboratory Rodents: Final Report of the American Institute of
Nutrition Ad Hoc Writing Committee on the Formulation of the AIN-
76A Rodent Diet ". J. Nutr., vol. 123, pp. 1939-1951, 1993.
[13] M.M. Benjamin. "Outline of Veterinary Chemical Pathology". 3rd
Edition. The Iowa State University Press, Iowa. USA, 1981.
[14] H. Debbabi, M. Dubbary, M. Rautureau, and D. Tome. "Bovine
Lactoferrin Induce both Mucosal and Systemic Immune Response in
Mice". Journal of Dairy Research, vol. 65, pp. 283-293, 1998.
[15] Nurliyani, E. Harmayani, and MHNE. Soesatyo. "The effect of goat
milk supplementation on Th1 and Th2 responses of Peyer patch
lymphocyte culture in dinitrochlorobenzene sensitized rat". Proceedings
of International Conference on Agricultural, Food and Animal Science.
Paris, July 28-30, 2010. World Academy of Science, Engineering and
Technology. Academic Science Research. Issue 67, ISSN 2070-3724.
[16] B.M. Ogilvie, M.H. Patricia, and M.E. Rose. "Nippostrongylus
brasiliensis: Peripheral blood leucocyte response of rats, with special
reference to basophils". Experimental Parasitology, vol. 46, pp. 20-30,
1978.
[17] Y. Li, L. Lixin, R. Wadley, S.W. Reddel, J.C. Qi, C. Archis, A.
Collins, E. Clark, M. Cooley, S. Kouts, H.M. Naif, M. Alali, A.
Cunningham, G.W. Wong, R.L. Stevens, and S.A. Krilis. "Mast
cells/basophils in peripheral blood of allergic individuals who are HIV-1
susceptible due to their surface expression of CD4 and the chemokine
receptors CCR3, CCR5, and CXCR4", Blood, vol. 97, no.11, pp. 3484-
3490, 2001
[1] J-S. Ham, J-H. Shin, J. Aera, S-G. Jeong, K-W. Park, H-U. Kim, S-Y.
Kang, H. J. Hwang, and W.K. Lee. "Effects of goat milk yogurt
supplemented with citrus concentrate on blood glucose and serum lipids
in diabetic rats". Korean J. Food Sci., vol. 28, no. 4, pp. 445-450, 2008.
[2] G.F. W. Haenlein. "Goat milk in human nutrition". Small Ruminant
Research, vol. 51, pp. 155-163, 2004.
[3] E.I. El-Agamy. The challenge of cow milk allergy. Small Ruminant
Research, vol. 68, pp. 64-72, 2007.
[4] C. Prosser, K. Stelwagen, R. Cummins, P. Guerin, N. Gill, and C.
Milne. "Reduction in heat-induced gastrointestinal hypermeability in
rats by bovine colostrum and goat milk powder". Journal of Applied
Physiology, vol. 96, pp. 650-654, 2003.
[5] F.Y. Wu, P.H. Tsao, D.C. Wang, S. Lin, J.S. Wu, and Y.K. Cheng.
"Factors affecting growth factor activity in goat milk", Journal of Dairy
Science 89: 1951-1955, 2006.
[6] A. Ghaffar. "Hypersensitivity reactions".
http://pathmicro.med.sc.edu/pdfimn2002/02Hypersensitivity.pdf, 2002
[7] H-O. Ku, S-H. Jeong, H-G, Kang, H-M. Pyo, J-H. Cho, S-W. Son, SM.
Yun, and D-Y. Ryu. "Gene expression profiles and pathways in skin
inflammation induced by three different sensitizers and an irritant".
Toxycology Letters, vol. 190, pp. 231-237, 2009
[8] M. Ban and D. Hettich. "Relationship between IgE possitive cell
numbers and serum total IgE levels in mice treated with trimellitic
anhydride and dinitrochlorobenzene". Toxicology letters, vol .118, pp.
129-137, 2001.
[9] C.F. Kuper, R.H. Stierum, A. Boorsma, M.A. Schij, M. Prinsen, J.P.
Bruijntjes, N. Bloksma, J.H.E. Arts. "The contact allergen
dinitrochlorobenzene (DNCB) and respiratory allergy in the Th2-prone
Brown Norway rat".Toxicology, vol. 246, pp. 213-221, 2008.
[10] O.B. Lim, K. Yamada, M. Nonaka, Y. Kuramoto, P. Hung, and M.
Sugano. "Dietary fiber modulate indices of intestinal immune function
in rats", The Journal of Nutrition, vol. 127, no.5, pp. 663-667, 1997.
[11] F.I. Aboderin and V.O. Oyetaya. "Haematological studies of rats fed
different doses of probiotic Lactobacillus plantarum, isolated from
fermenting corn slurry". Pakistan Journal of Nutrition, vol .5 , no. 2, pp.
102-105, 2006.
[12] P.G. Reeves, F.H. Neilsen, and G.C. Fahey, JR. "AIN-93 Purified Diets
for Laboratory Rodents: Final Report of the American Institute of
Nutrition Ad Hoc Writing Committee on the Formulation of the AIN-
76A Rodent Diet ". J. Nutr., vol. 123, pp. 1939-1951, 1993.
[13] M.M. Benjamin. "Outline of Veterinary Chemical Pathology". 3rd
Edition. The Iowa State University Press, Iowa. USA, 1981.
[14] H. Debbabi, M. Dubbary, M. Rautureau, and D. Tome. "Bovine
Lactoferrin Induce both Mucosal and Systemic Immune Response in
Mice". Journal of Dairy Research, vol. 65, pp. 283-293, 1998.
[15] Nurliyani, E. Harmayani, and MHNE. Soesatyo. "The effect of goat
milk supplementation on Th1 and Th2 responses of Peyer patch
lymphocyte culture in dinitrochlorobenzene sensitized rat". Proceedings
of International Conference on Agricultural, Food and Animal Science.
Paris, July 28-30, 2010. World Academy of Science, Engineering and
Technology. Academic Science Research. Issue 67, ISSN 2070-3724.
[16] B.M. Ogilvie, M.H. Patricia, and M.E. Rose. "Nippostrongylus
brasiliensis: Peripheral blood leucocyte response of rats, with special
reference to basophils". Experimental Parasitology, vol. 46, pp. 20-30,
1978.
[17] Y. Li, L. Lixin, R. Wadley, S.W. Reddel, J.C. Qi, C. Archis, A.
Collins, E. Clark, M. Cooley, S. Kouts, H.M. Naif, M. Alali, A.
Cunningham, G.W. Wong, R.L. Stevens, and S.A. Krilis. "Mast
cells/basophils in peripheral blood of allergic individuals who are HIV-1
susceptible due to their surface expression of CD4 and the chemokine
receptors CCR3, CCR5, and CXCR4", Blood, vol. 97, no.11, pp. 3484-
3490, 2001
@article{"International Journal of Biological, Life and Agricultural Sciences:60670", author = "Nurliyani and E. Harmayani and MHNE. Soesatyo", title = "The Effect of Goat Milk Fractions Supplementation on Serum IgE Response and Leukocytes Count in Dinitrochlorobenzene Sensitized Rat", abstract = "In Indonesia, goat milk is often consumed and
believed as anti-allergy. The objective of this research was to study
the effect of goat milk and their fractions (casein and whey)
supplementation on total serum IgE concentrations and leukocytes
count in rat sensitized with contact allergen dinitrochlorobenzene
(DNCB). Female Wistar rats 6-8 weeks old were divided into four
groups: 1) whey, 2) casein, 3) whole milk supplementation and 4)
phosphate-buffered saline/PBS (control). The results showed that
supplementation of goat milk on rats did not affects on total serum
IgE concentrations and number of leukocytes. After sensitized with
DNCB, the monocyte percentage in rats was higher (P", keywords = "Dinitrochlorobenzene, Goat Milk Fractions, IgE,
Leukocytes.", volume = "6", number = "10", pages = "927-4", }
