Effect of Dietary Supplementation of Different Levels of Black Seed (Nigella Sativa L.) on Growth Performance, Immunological, Hematological and Carcass Parameters of Broiler Chicks
This experiment was conducted to investigate the
effect of dietary supplementation of different levels of black seed
(Nigella sativa L.) on the performance and immune response of broiler chicks. A total 240 day-old broiler chicks were used and
randomly allotted equally into six experimental groups designated as 1, 2, 3, 4, 5 and 6 having black seed at the rate of 0, 2, 4, 6, 8 and
10 g /kg diet respectively. The study was lasted for 42 days. Average body weight, weight gain, relative growth rate, feed
conversion, antibody titer against Newcastle disease, phagocytic activity and phagocytic index, some blood parameters(GOT, GPT,
Glucose, Cholesterol, Triglyceride, Total protein, Albumen, WBCs,
RBCs, Hb and PCV), dressing percentage, weight of different body
organs, abdominal fat weight, were determined. It was found that, N. Sativa significantly improved final body weight, total body gain
and feed conversion ratio of groups 2 and 3 when compared with the control group. Higher levels of N. Sativa did not improve
growth performance of the chicks. Non significant differences were
observed for antibody titer against Newcastle virus, WBCs count,
serum GOT, glucose level, dressing %, relative liver, spleen, heart and head percentages. Lymphoid organs (Bursa and Thymus)
improved significantly with increasing N. Sativa level in all supplemented groups. Serum cholesterol, triglyceride and visible fat
% significantly decreased with Nigella sativa supplementation while
serum GPT level significantly increased with nigella sativa
supplementation.
[1] A. A. H. Tollbaand M. S. H. Hassan "Using some natural additives to improve physiological and productive performance of broiler chicks under high temperature conditions 2- black cumin (Nigella Sativa) or garlic (Allium sativum) ," Egypt. Poul. Sci. 2003, 23 (II):
(327-340).
[2] T. Guler, B. Dalkilic, O. N. Ertas, and M. Ciftci"The effect of dietary black cumin seeds (Nigella Sativa L.) on the performance of
broilers," Asian-australasian journal of animal sciences. 2006, 19:3, 425-430.
[3] F. R. Durrani, N. Chand , K. Zaka, A. Sultan , F. M. Khattak, and
Z. Durrani "Effect of different levels of feed added black seed (Nigella sativa L.) On the performance of broiler chicks," Pak J Biol
Sci. 2007, Nov 15; 10 (22):4164-7.
[4] A. A. Abdel-Hady, F. A. Abdel-Azeem, A. A. Abdel-Rafea, and A.
G. Gamal "Effect of replacement of soybean meal protein by nigella
sativa meal protein on performance of growing Japanese quail.
Egypt,"Poult. Sci. 2009, Vol (29) (I): (407-422).
[5] S. Mahmood, M. M. Hassan, M. Alam, and F. Ahmad
"Comparative efficacy of Nigella sativa and Allium sativum as
growth promoters in broilers," Int. J. Agric. Biol., 2009, 11: 775-778
[6] Ç. Mehmet, Y.
Black Seed Extract (Nigella sativa) on Growth Performance, Blood
Parameters, Oxidative Stress and DNA Damage of Partridges,"
Journal of Applied Animal Research,2008,Volume: 34, Issue : 2
Print ISSN : 0971-2119. Online ISSN: 0974-1844.
[7] Y. A. Attia, A. E. Tag El-Din, H. S. Zeweil, A. S. Hussein, E. M.
Qota, and M. A. Arafat "The effect of supplementation of enzyme
on laying and reproductive performance in Japanese quail hens fed
nigella seed meal," Journal of Poultry Science.2008, 45:110-115.
[8] E. S. Abd El-Aal, and R. S. Attia"Characterization of black cumin
(Nigella sativa), chemical composition and lipids," Alex. Sci. Exch.
1993, 14(4): 467-481.
[9] W. A. James, E. J. Amy, and R. M. Susan "Ten different dietary
fibers have significantly different effects on serum and liver lipids
of cholesterol-fed rats," J. Nutri. 1994, 124: 78-83.
[10] S. Hedaya,"Effects of Nigella sativa (balck seeds) extracts on some
hematological and biochemical parameters in rats," Alex. J. Vet.
Sci. 1995, 11(2): 95-99.
[11] N. A. AL-Beitawi, S. S. EL-Ghousein, and A. H. Nofal "Replacing
bacitracin methylene disalicylate by crushed Nigella sativa seeds in
broiler rations and its effects on growth, blood constituents and immunity,"Livestock Science. 2009, Volume 125, Issues 2-3, Pages
304-307.
[12] M. Toghyani, M. Toghyani, A. Gheisari, G. Ghalamkari, and M.
Mohammadrezaei"Growth performance, serum biochemistry and
blood hematology of broiler chicks fed different levels of black seed (Nigella sativa) and peppermint (Menthapiperita)," Livestock
Science.2010,129, 173-178
[13] A. Al-Homidan, A. Al-Qarawi, S. A. Al-Waily, and S. E.
Adam"Response of broiler chicks to dietary Rhazyastricta and
Nigella sativa,"Br Poult Sci.2002, 43(2):291-6.
[14] M. S. H. Hassan, A. M. AboTaleb, M.M. Wakwak, and B.A. Yousef
"Productive, physiological and immunological effect of using some natural feed additives in Japanese quail,"Egypt.Poul.Sci. 2007,
27(II):557-581.
[15] NRC,"National Research Council: Nutrient requirements of
poultry,"1994, 9thed National Academy press, Washington,
DC.
[16] E. W. Crampton, and L. E. Lioyd"Fundamentals of
Nutrition,"1959, W. H. Free and Company. San Francisco
and London.
[17] W. V. Lambert, N. R. Ellis, W. H. Block, and H. W. Titus
"The role of nutrition in genetics," Am. Res. Soc Animal
prod. 1936, 29: 236.
¶ÇÇà¶ÇÇü¶ÇÇé¶ÇÇå G. Y. Takatasy,"The use of spiral 100 Min Serological and
Virologicalmicromethods,"Acta, Microbial, Acad. Sci. Hung.
1955, 3:191.
¶ÇÇà¶ÇÇü¶ÇÇä¶ÇÇå M. G. Brugh, "A simple method for recording and analyzing
serological data," Avian Disease.1978, 22: 362-365.
[20] E. Kawahara, T. Ueda, and S. Nomura "In vitro phagocytic activity
of white spotted shark cells after injection with
Aeromoassalmonicidaextraceular products,"Gyobyo ,Kenkyu,
Japan, 1991, 26(4):213-214.
[21] S. Reitman, and S. Frankel "A colorimetric method for
determination of serum glutamic oxaloacetic and glutamic pyruvic
transaminase," Am. J. Path. 1957, 26: 1-13.
[22] P. Trinder, "Determination of glucose in blood using glucose
oxidase with alternative oxygen acceptor," Ann. Clin. Biochm.
1969, 6: 24.
[23] B. Zak, R.Dickenman, E. White, H. Burnet, and P. Cherney"Rapid
estimation of free and total cholesterol," Am. J. Clin. Path. 1954,
24: 1307.
[24] P. G. Sidney, and R. Barnard "Improved manual spectrophotometric
procedure for determination of serum triglycerides,"Clin. Chem.
1973, 19 (9): 1077-1078.
[25] B. T.Doumas, D. D. Bayso, R. J. Carter, T. Peters, and R. Schaffer
"Determination of total serum protein,"Clin. Chem. 1981, 27:1642-
1643.
[26] R. R. Reinhold, "Determination of serum albumin,"Clin. Chem.
1953, 21: 1370 -1372.
[27] M. Maxine, and B. S. Benjamin "Outline of veterinary clinical
pathology,"1985, 3rd Ed., Colorado State University.
[28] Z. Lucky, "Methods for diagnosis of fish disease,"Ameruno
publishing Co, PVT, Ltd New Delhi, Bomby, New York, 1977.
[29] Statistical Analysis System (SAS) "Statistical user-s
Guide,".2004, INT., Cary, NC. USA.
[30] H. M. Ziad, Abu-Dieyeh, and S. A. Mohammad "Effect of feeding
powdered Black Cumin Seeds (Nigella Sativa L.) on growth
performance of 4- weeks- old Broilers," Journal of Animal and
Veterinary Advances. 2008, 7, (3): 286-290.
[31] G. Erener, N. Altop, H. Ocak, S. Aksoy, and E.
Ozturk,"Influence of black cumin seed (Nigella sativa L.) and
seed extract on broilers performance and total coliform
bacteria count," Asian J. Anim. Vet. Adv. 2010, 5: 128-135.
[32] N. I. Hassanin, and F.M. Hassan,"A preliminary study on the effect
of Nigella sativa L.seeds on hypoglycemia," Vet. Med. J. Giza.1996,
44:4, 699-708.
[33] M. El-Dakhakhny, N. Mady, N. Lembert, and H.P.T. Ammon,"The
hypoglycemic effect of Nigella sativa oil is mediated by
extrapancreatic actions,". Plant. Medica. 2002, 68: 5, 465-466;6 ref.
[34] A. Zaoui, Y.P.Cherrah,K. Alaoui, N. Mahassine, L.T. Amarouch,
and M. Hassar,"Effectes of Nigella sativa fixed oil on blood
homeostasis in rat,"J.Ethnopharmacol Jan. 2002; 79:1, 23-26.
[35] K. M. Fararh, Y. Atoji, Y. Shimizu, and T.
Takewaki,"Insulinotropic properties of Nigella sativa oil in
streptozotocin plus Nicotinamiede diabetic hamster,". Research in
Veterinary Science. 2002,73: 3, 279-282.
[36] I. Meral, N. Donmez, B. Baydas, F. Belge, and M. Kanter,"Effect
of Nigella sativa L. on heart rate and some haematological values of
alloxan induced diabetic rabbits," Scandinavian Journal of
Laboratory Animal science. 2004, 31: 1, 49-53.
[37] N. A.AL-Beitawi, and S.S. El-Ghousein,"Effect of Feeding Different Levels of Nigella sativa Seeds (Black Cumin) on Performance, Blood Constituents and Carcass Characteristics of
Broiler Chicks," International Journal of Poultry Science.2008, 7,(7): 715-721.
[1] A. A. H. Tollbaand M. S. H. Hassan "Using some natural additives to improve physiological and productive performance of broiler chicks under high temperature conditions 2- black cumin (Nigella Sativa) or garlic (Allium sativum) ," Egypt. Poul. Sci. 2003, 23 (II):
(327-340).
[2] T. Guler, B. Dalkilic, O. N. Ertas, and M. Ciftci"The effect of dietary black cumin seeds (Nigella Sativa L.) on the performance of
broilers," Asian-australasian journal of animal sciences. 2006, 19:3, 425-430.
[3] F. R. Durrani, N. Chand , K. Zaka, A. Sultan , F. M. Khattak, and
Z. Durrani "Effect of different levels of feed added black seed (Nigella sativa L.) On the performance of broiler chicks," Pak J Biol
Sci. 2007, Nov 15; 10 (22):4164-7.
[4] A. A. Abdel-Hady, F. A. Abdel-Azeem, A. A. Abdel-Rafea, and A.
G. Gamal "Effect of replacement of soybean meal protein by nigella
sativa meal protein on performance of growing Japanese quail.
Egypt,"Poult. Sci. 2009, Vol (29) (I): (407-422).
[5] S. Mahmood, M. M. Hassan, M. Alam, and F. Ahmad
"Comparative efficacy of Nigella sativa and Allium sativum as
growth promoters in broilers," Int. J. Agric. Biol., 2009, 11: 775-778
[6] Ç. Mehmet, Y.
Black Seed Extract (Nigella sativa) on Growth Performance, Blood
Parameters, Oxidative Stress and DNA Damage of Partridges,"
Journal of Applied Animal Research,2008,Volume: 34, Issue : 2
Print ISSN : 0971-2119. Online ISSN: 0974-1844.
[7] Y. A. Attia, A. E. Tag El-Din, H. S. Zeweil, A. S. Hussein, E. M.
Qota, and M. A. Arafat "The effect of supplementation of enzyme
on laying and reproductive performance in Japanese quail hens fed
nigella seed meal," Journal of Poultry Science.2008, 45:110-115.
[8] E. S. Abd El-Aal, and R. S. Attia"Characterization of black cumin
(Nigella sativa), chemical composition and lipids," Alex. Sci. Exch.
1993, 14(4): 467-481.
[9] W. A. James, E. J. Amy, and R. M. Susan "Ten different dietary
fibers have significantly different effects on serum and liver lipids
of cholesterol-fed rats," J. Nutri. 1994, 124: 78-83.
[10] S. Hedaya,"Effects of Nigella sativa (balck seeds) extracts on some
hematological and biochemical parameters in rats," Alex. J. Vet.
Sci. 1995, 11(2): 95-99.
[11] N. A. AL-Beitawi, S. S. EL-Ghousein, and A. H. Nofal "Replacing
bacitracin methylene disalicylate by crushed Nigella sativa seeds in
broiler rations and its effects on growth, blood constituents and immunity,"Livestock Science. 2009, Volume 125, Issues 2-3, Pages
304-307.
[12] M. Toghyani, M. Toghyani, A. Gheisari, G. Ghalamkari, and M.
Mohammadrezaei"Growth performance, serum biochemistry and
blood hematology of broiler chicks fed different levels of black seed (Nigella sativa) and peppermint (Menthapiperita)," Livestock
Science.2010,129, 173-178
[13] A. Al-Homidan, A. Al-Qarawi, S. A. Al-Waily, and S. E.
Adam"Response of broiler chicks to dietary Rhazyastricta and
Nigella sativa,"Br Poult Sci.2002, 43(2):291-6.
[14] M. S. H. Hassan, A. M. AboTaleb, M.M. Wakwak, and B.A. Yousef
"Productive, physiological and immunological effect of using some natural feed additives in Japanese quail,"Egypt.Poul.Sci. 2007,
27(II):557-581.
[15] NRC,"National Research Council: Nutrient requirements of
poultry,"1994, 9thed National Academy press, Washington,
DC.
[16] E. W. Crampton, and L. E. Lioyd"Fundamentals of
Nutrition,"1959, W. H. Free and Company. San Francisco
and London.
[17] W. V. Lambert, N. R. Ellis, W. H. Block, and H. W. Titus
"The role of nutrition in genetics," Am. Res. Soc Animal
prod. 1936, 29: 236.
¶ÇÇà¶ÇÇü¶ÇÇé¶ÇÇå G. Y. Takatasy,"The use of spiral 100 Min Serological and
Virologicalmicromethods,"Acta, Microbial, Acad. Sci. Hung.
1955, 3:191.
¶ÇÇà¶ÇÇü¶ÇÇä¶ÇÇå M. G. Brugh, "A simple method for recording and analyzing
serological data," Avian Disease.1978, 22: 362-365.
[20] E. Kawahara, T. Ueda, and S. Nomura "In vitro phagocytic activity
of white spotted shark cells after injection with
Aeromoassalmonicidaextraceular products,"Gyobyo ,Kenkyu,
Japan, 1991, 26(4):213-214.
[21] S. Reitman, and S. Frankel "A colorimetric method for
determination of serum glutamic oxaloacetic and glutamic pyruvic
transaminase," Am. J. Path. 1957, 26: 1-13.
[22] P. Trinder, "Determination of glucose in blood using glucose
oxidase with alternative oxygen acceptor," Ann. Clin. Biochm.
1969, 6: 24.
[23] B. Zak, R.Dickenman, E. White, H. Burnet, and P. Cherney"Rapid
estimation of free and total cholesterol," Am. J. Clin. Path. 1954,
24: 1307.
[24] P. G. Sidney, and R. Barnard "Improved manual spectrophotometric
procedure for determination of serum triglycerides,"Clin. Chem.
1973, 19 (9): 1077-1078.
[25] B. T.Doumas, D. D. Bayso, R. J. Carter, T. Peters, and R. Schaffer
"Determination of total serum protein,"Clin. Chem. 1981, 27:1642-
1643.
[26] R. R. Reinhold, "Determination of serum albumin,"Clin. Chem.
1953, 21: 1370 -1372.
[27] M. Maxine, and B. S. Benjamin "Outline of veterinary clinical
pathology,"1985, 3rd Ed., Colorado State University.
[28] Z. Lucky, "Methods for diagnosis of fish disease,"Ameruno
publishing Co, PVT, Ltd New Delhi, Bomby, New York, 1977.
[29] Statistical Analysis System (SAS) "Statistical user-s
Guide,".2004, INT., Cary, NC. USA.
[30] H. M. Ziad, Abu-Dieyeh, and S. A. Mohammad "Effect of feeding
powdered Black Cumin Seeds (Nigella Sativa L.) on growth
performance of 4- weeks- old Broilers," Journal of Animal and
Veterinary Advances. 2008, 7, (3): 286-290.
[31] G. Erener, N. Altop, H. Ocak, S. Aksoy, and E.
Ozturk,"Influence of black cumin seed (Nigella sativa L.) and
seed extract on broilers performance and total coliform
bacteria count," Asian J. Anim. Vet. Adv. 2010, 5: 128-135.
[32] N. I. Hassanin, and F.M. Hassan,"A preliminary study on the effect
of Nigella sativa L.seeds on hypoglycemia," Vet. Med. J. Giza.1996,
44:4, 699-708.
[33] M. El-Dakhakhny, N. Mady, N. Lembert, and H.P.T. Ammon,"The
hypoglycemic effect of Nigella sativa oil is mediated by
extrapancreatic actions,". Plant. Medica. 2002, 68: 5, 465-466;6 ref.
[34] A. Zaoui, Y.P.Cherrah,K. Alaoui, N. Mahassine, L.T. Amarouch,
and M. Hassar,"Effectes of Nigella sativa fixed oil on blood
homeostasis in rat,"J.Ethnopharmacol Jan. 2002; 79:1, 23-26.
[35] K. M. Fararh, Y. Atoji, Y. Shimizu, and T.
Takewaki,"Insulinotropic properties of Nigella sativa oil in
streptozotocin plus Nicotinamiede diabetic hamster,". Research in
Veterinary Science. 2002,73: 3, 279-282.
[36] I. Meral, N. Donmez, B. Baydas, F. Belge, and M. Kanter,"Effect
of Nigella sativa L. on heart rate and some haematological values of
alloxan induced diabetic rabbits," Scandinavian Journal of
Laboratory Animal science. 2004, 31: 1, 49-53.
[37] N. A.AL-Beitawi, and S.S. El-Ghousein,"Effect of Feeding Different Levels of Nigella sativa Seeds (Black Cumin) on Performance, Blood Constituents and Carcass Characteristics of
Broiler Chicks," International Journal of Poultry Science.2008, 7,(7): 715-721.
@article{"International Journal of Biological, Life and Agricultural Sciences:58246", author = "R. S. Shewita and A. E. Taha", title = "Effect of Dietary Supplementation of Different Levels of Black Seed (Nigella Sativa L.) on Growth Performance, Immunological, Hematological and Carcass Parameters of Broiler Chicks", abstract = "This experiment was conducted to investigate the
effect of dietary supplementation of different levels of black seed
(Nigella sativa L.) on the performance and immune response of broiler chicks. A total 240 day-old broiler chicks were used and
randomly allotted equally into six experimental groups designated as 1, 2, 3, 4, 5 and 6 having black seed at the rate of 0, 2, 4, 6, 8 and
10 g /kg diet respectively. The study was lasted for 42 days. Average body weight, weight gain, relative growth rate, feed
conversion, antibody titer against Newcastle disease, phagocytic activity and phagocytic index, some blood parameters(GOT, GPT,
Glucose, Cholesterol, Triglyceride, Total protein, Albumen, WBCs,
RBCs, Hb and PCV), dressing percentage, weight of different body
organs, abdominal fat weight, were determined. It was found that, N. Sativa significantly improved final body weight, total body gain
and feed conversion ratio of groups 2 and 3 when compared with the control group. Higher levels of N. Sativa did not improve
growth performance of the chicks. Non significant differences were
observed for antibody titer against Newcastle virus, WBCs count,
serum GOT, glucose level, dressing %, relative liver, spleen, heart and head percentages. Lymphoid organs (Bursa and Thymus)
improved significantly with increasing N. Sativa level in all supplemented groups. Serum cholesterol, triglyceride and visible fat
% significantly decreased with Nigella sativa supplementation while
serum GPT level significantly increased with nigella sativa
supplementation.", keywords = "Nigella Sativa, broiler, growth, carcass traits, serum, blood", volume = "5", number = "5", pages = "290-7", }
