Abstract: The main objective of this study was to determine the
effects of Nitrogen fixing bacteria and manure application on the seed
yield and yield components in black cumin (Nigella sativa L.). The
experiment was carried out at the RAN Research Station in
Firouzkouh in 2012. A 4×4 factorial experiment, arranged in a
randomized complete blocks designed with three replications.
Nitrogen fixing bacteria at 4 levels (control, Azotobacter,
Azospirillum and Azotobacter + Azospirillum) and manure
application at 4 levels (0, 2.5, 5 and 7.5 ton ha-1) were used at this
investigation. The present results have shown that the highest height,
1000 seeds weight, seed number per follicle, follicle yield, seed yield
and harvest index were obtained after using Azotobacter and
Azospirillum, simultaneously. Manure application only effects on
follicle yield and by 5ton manure ha-1 the highest follicle yield
obtained. Results of this investigation showed that the maximum seed
yield obtained when Aotobacter+Azospirillum inoculated with black
cumin seeds and 5 ton manure ha-1 applied. According to the results
of this investigation the integrated management of Azotobacter and
Azospirillum with manure application is the best treatment for
achieving the maximum quantitative charactersitics of Black cumin.
Abstract: Indian subcontinent has a plethora of traditional
medicine systems that provide promising solutions to lifestyle
disorders in an 'all natural way'. Spices and oilseeds hold
prominence in Indian cuisine hence the focus of the current study
was to evaluate the bioactive molecules from Linum usitatissinum
(LU), Lepidium sativum (LS), Nigella sativa (NS) and Guizotia
abyssinica (GA) seeds. The seeds were characterized for functional
lipids like omega-3 fatty acid, antioxidant capacity, phenolic
compounds, dietary fiber and anti-nutritional factors. Analysis of the
seeds revealed LU and LS to be a rich source of α-linolenic acid
(41.85 ± 0.33%, 26.71 ± 0.63%), an omega 3 fatty acid (using
GCMS). While studying antioxidant potential NS seeds demonstrated
highest antioxidant ability (61.68 ± 0.21 TEAC/ 100 gm DW) due to
the presence of phenolics and terpenes as assayed by the Mass
spectral analysis. When screened for anti-nutritional factor
cyanogenic glycoside, LS seeds showed content as high as 1674 ± 54
mg HCN / kg. GA is a probable good source of a stable vegetable oil
(SFA: PUFA 1:2.3). The seeds showed diversified bioactive profile
and hence further studies to use different bio molecules in tandem for
the development of a possible 'nutraceutical cocktail' have been
initiated..
Abstract: Antifungal activities of ether and methanolic extracts of volatiles oils of Nigella Sativa seeds were tested against pathogenic bacterias and fungies strains.The volatile oil were found to have significant antifungal and antibacterial activities compare to tetracycline, cefuroxime and ciprofloxacin positive controls.The ether and methanolic esxtracts were compared to each other for antifungal and antibacterial activities and ether extracts showed stonger activity than methanolic one.
Abstract: Surgical site infections (SSIs) are the most common
nosocomial infection in surgical patients resulting in significant
increases in postoperative morbidity and mortality. The commonly
causative bacteria developed resistance to virtually all antibiotics
available. The aim of this study was to isolation and identification the
most common bacteria that cause SSIs in Medical Research Institute,
and to compare their sensitivity to selected group of antibiotics and
natural products (garlic, oregano, olive, and Nigella sativa oils). The
isolated pathogens collected from infected surgical wounds were
identified, and their sensitivities to the antibiotics commonly
available for clinical use, and also to the different concentrations of
the used natural products were investigated. The results indicate to
the potential therapeutic effect of the tested natural products in
treatment of surgical wound infections.
Abstract: This experiment was conducted to investigate the
effect of dietary supplementation of different levels of black seed
(Nigella sativa L.) on the performance and immune response of broiler chicks. A total 240 day-old broiler chicks were used and
randomly allotted equally into six experimental groups designated as 1, 2, 3, 4, 5 and 6 having black seed at the rate of 0, 2, 4, 6, 8 and
10 g /kg diet respectively. The study was lasted for 42 days. Average body weight, weight gain, relative growth rate, feed
conversion, antibody titer against Newcastle disease, phagocytic activity and phagocytic index, some blood parameters(GOT, GPT,
Glucose, Cholesterol, Triglyceride, Total protein, Albumen, WBCs,
RBCs, Hb and PCV), dressing percentage, weight of different body
organs, abdominal fat weight, were determined. It was found that, N. Sativa significantly improved final body weight, total body gain
and feed conversion ratio of groups 2 and 3 when compared with the control group. Higher levels of N. Sativa did not improve
growth performance of the chicks. Non significant differences were
observed for antibody titer against Newcastle virus, WBCs count,
serum GOT, glucose level, dressing %, relative liver, spleen, heart and head percentages. Lymphoid organs (Bursa and Thymus)
improved significantly with increasing N. Sativa level in all supplemented groups. Serum cholesterol, triglyceride and visible fat
% significantly decreased with Nigella sativa supplementation while
serum GPT level significantly increased with nigella sativa
supplementation.
Abstract: Nigella sativa L. is an aromatic plant belonging to the
family Ranunculaceae. It has been used traditionally, especially in the
middle East and India, for the treatment of asthma, cough, bronchitis,
headache, rheumatism, fever, influenza and eczema. Several
biological activities have been reported in Nigella sativa seeds,
including antioxidant. In this context we tried to estimate the
antioxidant activity of various extracts prepared from Nigella sativa
seeds, methanolic extract (ME), chloroformic extract (CE), hexanic
extract (HE : fixed oil), ethyl acetate extract (EAE) water extract
(WE). The Folin-Ciocalteu assay showed that CE and EAE contained
high level of phenolic compounds 81.31 and 72.43μg GAE/mg of
extract respectively. Similarly, the CE and EAE exhibited the highest
DPPH radical scavenging activity, with IC50 values of 106.56μg/ml
and 121.62μg/ml respectively. In addition, CE and HE showed the
most scavenging activity against superoxide radical generated in the
PMS-NADH-NBT system with respective IC50 values of 361.86
μg/ml and 371.80 μg/ml, which is comparable to the activity of the
standard antioxidant BHT (344.59 μg/ml). Ferrous ion chelating
capacity assay showed that WE, EAE and ME are the most active
with 40.57, 39.70 and 22.02 mg EDTA-E/g of extract. The inhibition
of linoleic acid/ß-carotene coupled oxidation was estimated by ßcarotene
bleaching assay, this showed a highest relative antioxidant
activity with CE and EAE (69.82% of inhibition). The antioxidant
activities of the methanolic extract and the fixed oil are confirmed by
an in vivo assay in mice, the daily oral administration of methanolic
extract (500 and 800 mg/kg/day) and fixed oil (2 and 4 ml/kg/day)
during 21 days, resulted in a significant enhancement of the blood
total antioxidant capacity (measured by KRL test) and the plasmatic
antioxidant capacity towards DPPH radical.