Detection of Pathogenic Escherichia coli Strains Pollution in Red Deer Meat in Latvia and Determination the Compatibility of VT1, VT2, eae A Genes in their Isolate

Tasks of the work were study the possible E.coli contamination in red deer meat, identify pathogenic strains from isolated E.coli, determine their incidence in red deer meat and determine the presence of VT1, VT2 and eaeA genes for the pathogenic E.coli. 8 (10%) samples were randomly selected from 80 analysed isolates of E.coli and PCR reaction was performed on them. PCR was done both on initial materials – samples of red deer meat - and for already isolated liqueurs. Two of analysed venison samples contain verotoxin-producing strains of E. coli. It means that this meat is not safe to consumer. It was proven by the sequestration reaction of E. coli and by comparison of the obtained results with the database of microorganism genome available on the internet that the isolated culture corresponds to region 16S rDNS of E. coli thus presenting correctness of the microbiological methods.

Authors:

• S. Liepina
• A. Jemeljanovs

Keywords:

• Deer meat
• pathogenic Escherichia coli

References:

[1] L. W. Riley, R. S. Remis, S. D. Helgerson, H. B. McGee, J. G. Wells,
B. R. Davis, R. J. Herbert, E. S. Olcott, L. M. Johnson, N.T. Hargrett,
P. A. Blake, and M. L. Cohen, Haemorrhagic colitis associated with an
Escherichia coli serotype New England Journal of Medicine, vol. 308,
24, March. 1983, pp. 681-685.
[2] H. Karch, P. I. Tarr and M. Bielaszewska, Enterohaemorrhagic
Escherichia coli in human medicine International Journal of Medical
Microbiology, vol. 295, Isuue 6-7, Oct. 2005, pp. 405 -418.
[3] Ch. Bindu Kiranmayi, N. Krishnaiah, and E. Naga Mallika Escherichia
coli O157:H7 - an emerging pathogen in foods of animal origin.
Veterinary World, vol. 3, No. 8, Aug. 2010, pp. 382-389.
[4] A. V. Tutenel, D. Pierard, J. Van Hoof, M. Cornelis, and L. De Zutter,
ÔÇ×Isolation and molecular characterization of Escherichia coli O157
isolated from cattle, pigs and chickens at slaughter International Journal
of Food Microbiology, vol. 84, issue 1, 15, July. 2003, pp. 63-69
[5] M. A. Karmali, V. Gannon and J. M. Sargeant Verocytotoxin producing
Escherichia coli (VTEC). Veterinary Microbiology 140, 2010, pp. 360-
370.
[6] N. A. Strockbine, J. G. Wells, C. A. Bopp, and T. J. Barrett, "Overview
of detection and subtyping methods, in Escherichia coli O157:H7 and
Other Shiga Toxin-Producing E. Coli Strains, J.B Kaper., A.D.
O-Brien.Ed. Washington, American Society for Microbiology Press,
1998, pp. 331-356.
[7] M. Blanco, J. E. Blanco, A. Mora, G. Dahbi, M. P. Alonso, E. A.
Gonzalez, M. I. Bernardez, and J. Blanco, Serotypes, virulence genes,
and intimin types of Shiga toxin (verotoxin) - producing Escherichia coli
isolates from cattle in Spain and identification of a new intimin variant
gene Journal of Clinical Microbiology 42, 2004, pp. 645-651.
[8] J. C. Paton, and A. W. Paton, Pathogenesis and diagnosis of Shiga
toxin-producing Escherichia coli infections. Clinical Microbiology
Reviews, vol.11, issue 3, July. 1998, pp. 450-479.
[9] E. Lahti, M. Keskimaki, L. Rantala, P. Hyvonen, A. Siitonen and T.
Honkanen-Buzalski Occurrence of Escherichia coli O157 in Finish
cattle. Veterinary Microbiology, vol. 79, issue 3, Apr. 2001, pp. 239-
251.
[10] A. García-Sánchez, S. Sánchez, R. Rubio, G. Pereira, J.M. Alonso, J.
Hermoso de Mendoza, and J. Rey Presence of Shiga toxin-producing
E.coli 157:H7 in a survey of wild artiodactyls. Veterinary Microbiology,
vol.121, issue 3/4, Apr. 2007, pp. 373-377.
[11] J. M. Sargeant, D. J. Hafer, J. R. Gillespie, R. D. Oberst, and S.J. Flood
Prevalence of Escherichia coli O157:H7 in white-tailed deer sharing
rangeland with cattle. Journal of the American Veterinary Medical
Association, vol. 215, No.5, 1999, pp. 792-794.
[12] J. W. Yoon, and C. J. Hovde, "All blood, no stool: enterohemorrhagic
Escherichia coli O157:H7 infection Journal Veterinary Science 9, 2008,
pp. 219-231
[13] T. Rabatsky-Ehr, D. Dingman, R. Marcus, R. Howard, R. Kinney, and
P. Mshar. Deer meat as the source for a sporadic case of Escherichia coli
O157:H7 infection. Emerging Infectious Diseases, 8 (5), 2002, pp. 525-
527.
[14] A. Miko, K. Pries, S. Haby, K. Steege, N. Albrecht, G. Krause, and L.
Beutin. Assessment of Shiga toxin-producing Escherichia coli isolates
from wildlife meat as potential pathogens for humans. Applied and
Environmental. Microbiology. 75, 2009, pp. 6462-6470.
[15] S. Lehmann, M. Timm, H. Steinruck, and P. Gallien. Nachweis von
STEC im Kot von Hochwild und in Wildfleischwirtschaft.
Fleischwirtschaft, vol. 86, No. 4, 2006, s. 93 -96.
[16] M. Louie, J. C. S. De Azavedo, M.Y. Handelsman, C. G. Clark, B. Ally,
M. Dytoc, P. Sherman, J. Brunton. Expression and Characterization of
the eaeA Gene Product of Escherichia coli Serotype 0157:H7. Infection
and Immunity, vol.61, No.10, Oct. 1993, pp. 4085-4092.
[17] R. A. Hazarika, D. K. Singh, K. N. Kapoor, R. K. Agarwal, A. B.
Pandey, and D.N. Rajkumar. Detection and characterization of
verotoxin-producing Escherichia coli (VTEC) isolated from buffalo
meat. Journal of Food Safety, vol. 24, issue 4, Nov. 2004, pp. 281-290.
[18] M. Samadpour, J. E. Ongerth, J. Liston, N. Tran, D. Nguyen, T. S.
Whittam, R. A. Wilson, and P.I. Tarr Occurrence of Shiga-like toxinproducing
Escherichia coli in retail fresh seafood, beef, lamb, porkand
poultry from grocery stores in Seattle, Washington. Applied and
Environmental. Microbiology, vol. 60, No. 3, March. 1994, pp.1038-
1040.
[19] C. A. Philips. The epidemiology, detection and control of Escherichia
coli O157. Journal of the Science of Food and Agriculture, vol. 79, issue
11, Aug. 1999, pp.1367-1381.
[20] J. S. Meng, T. Zhao, and M.P. Doyle Virulence genes of Shiga toxin
producing Escherichia coli isolated from food, animals and humans.
International Journal of Food Microbiology, vol. 45, issue 3 22, Dec.
1998, pp. 229-235.
[21] J. L. Holland, L. Louie, A. E. Simor, and M. Louie. PCR detection of
Escherichia coli O157:H7 directly from stools: evaluatiuon of
commercial extraction methods for purifying fecal DNA. Journal of
Clinical Microbiology, vol. 38, issue 11, Nov. 2000, pp. 4108-4113.
[22] E. J. Kee, and R.O. Elder. (2002) Isolation of Shiga-toxigenic
Escherichia coli O157 from hide surface and the oral cavity of finished
beef feedlot cattle. Journal of the American Veterinary Medical
Association, vol. 220, No. 6, March. 2002, pp.756-763.
[23] A. E. Heuvelink, F. E. Van Den Biggelaar, E. De Boer, R. G. Herbes,
W. J. Melche, J. H. Huis, and L.A. Monnens L.A. Isolation and
characterization of verocytotoxin-producing Escherichia coli O157
strains from Dutch cattle and sheep. Journal of Clinical Microbiology,
Vol.36, issue 4, Apr. 1998, pp. 878-882.
[24] G. A. Barkocy-Gallagher, T. M. Arthur, G.R. Siragusa, J. E. Keen, R. O.
Elder, W.W. Laegreid, and M. Koohmaraie Genotypic analyses of
Escherichia coli O157:H7 and O157nonmotil isolates recovered from
beef cattle and carcasses at processing plants in the Midwestern of the
United States. Applied and Environmental Microbiology, vol. 67, No. 9,
Sept. 2001, pp. 3810-3818.
[25] P. A. Chapman, A. T. Cerdan Malo, M. Ellin, R. Ashton, and M.A.
Harkin M.A. Escherichia coli O157 in cattle and sheep at slaughter, on
beef and lamb carcasses and in raw beef and lamb products in South
Yorkshire,UK. International Journal of Food Microbiology, vol. 64,
issue1/2 28, Febr. 2001, pp. 139-150.
[26] F. Rogerie, A. Marecat, S. Gambade, F. Dupond, P. Beaubois and M.
Lange Characterization of Shiga toxin producing E. Coli and O157
serotype E. Coli isolated in France from healthy domestic cattle.
International Journal of Food Microbiology, vol.63, issue 3, 15, Febr.
2001, pp. 217-223.
[27] E. M. Nielsen, and F. Scheutz. Characterisation of Escherichia coli O157
isolates from Danish cattle and human patients by genotyping and
presence and variants of virulence genes. Veterinary Microbiology, vol.
88, issue 3, Sept. 2002, pp. 259-273.
[28] F. Omisakin, M. Macrae, I. D. Ogden, and N. J. Strachan Concentration
and prevalence of Escherichia coli O157 in cattle feces at slaughter.
Applied and Environmental. Microbiology, vol.69, No. 5, May. 2003,
pp. 2444-2447.