Wheat Bran Carbohydrates as Substrate for Bifidobacterium lactis Development

The present study addresses problems and solutions related to new functional food production. Wheat (Triticum aestivum L) bran obtained from industrial mill company “Dobeles dzirnavieks”, was used to investigate them as raw material like nutrients for Bifidobacterium lactis Bb-12. Enzymatic hydrolysis of wheat bran starch was carried out by α-amylase from Bacillus amyloliquefaciens (Sigma Aldrich). The Viscozyme L purchased from (Sigma Aldrich) were used for reducing released sugar. Bifidibacterium lactis Bb-12 purchased from (Probio-Tec® CHR Hansen) was cultivated in enzymatically hydrolysed wheat bran mash. All procedures ensured the number of active Bifidobacterium lactis Bb-12 in the final product reached 105 CFUg-1. After enzymatic and bacterial fermentations sample were freeze dried for analysis of chemical compounds. All experiments were performed at Faculty of Food Technology of Latvia University of Agriculture in January- March 2013. The obtained results show that both types of wheat bran (enzymatically treated and non-treated) influenced the fermentative activity and number of Bifidibacterium lactis Bb-12 viable in wheat bran mash. Amount of acidity strongly increase during the wheat bran mash fermentation. The main objective of this work was to create low-energy functional enzymatically and bacterially treated food from wheat bran using enzymatic hydrolysis of carbohydrates and following cultivation of Bifidobacterium lactis Bb-12.

Allelopathic Effects of Sisymbrium irio L. and Descurainia sophia (L.) Schur on the Germination of Wheat (Triticum aestivum L.)

An experiment was conducted under controlled conditions to study the effect of water extract of leaves, shoots and roots of either Sisymbrium irio L. =SISIR and/or Descurainia sophia (L.) Schur =DESSO on the germination and primary growth of wheat. A split-split plot experiment in CRD with three replications was used. The main plots were the type of weed: i.e. SISIR and DESSO and the sub-plots were type of organ: i.e. leaf, stem and root and, the sub-sub plots were concentration of the water extract of each organ of the weeds: i.e. 0, 2, 4 and 8 % w/v. The plant materials were cut in 2-3 cm pieces and then were ground in a blender. The crushed materials were weighed according to experimental protocol and the final volume was reached to 100 ml in distilled water in dark bottles. All bottles were put on a shaker for 24 hours. The solutions were filtered by muslin cloth. Whatman paper, 9 cm in diameter, were put in petri dishes and twenty seeds of wheat were put on it and 5 ml distilled water or water extract of weeds were added to each petri dish. All petri dishes were put in constant temperature of 15 0C incubator. The results showed that the SISIR water extract had a greater inhibitory effects on germination and primary growth of wheat than those of DESSO water extract. The water extracts of the leaves of both weeds had the greatest inhibitory effects on germination and primary growth of wheat, compared to those of stems and roots. Increasing the concentration of water extract of leaves, stems and roots of both weeds up to 8 % caused the greatest inhibitory effects to wheat and reduced the germination rate and primary growth of it linearly.

Determination of Alkaline Protease Production In Serratia Marcescens Sp7 Using Agro Wastes As Substrate Medium, Optimization Of Production Parameters And Purification Of The Enzyme

The enzyme alkaline protease production was determined under solid state fermentation using the soil bacteria Serratia marcescens sp7. The maximum production was obtained from wheat bran medium than ground nut shell and chemically defined medium. The physiological fermentation factors such as pH of the medium (pH 8), Temperature (40oC) and incubation time (48 hrs) played a vital role in alkaline protease production in all the above. 100Mm NaCl has given better resolution during elution of the enzymes. The enzyme production was found to be associated with growth of the bacterial culture.

Sulfamonomethoxine-Induced Urinary Calculiin Pigs

The authors report a case of swine urolithiasis caused by improper administration of sulfamonomethoxine and which was diagnosed by examination of urinary sediments and analyzing the composition of the uroliths. The chemical composition of urinary calculi obtained from affected pigs with urolithiasis was further confimed as sulfamonomethoxine by fourier transform infrared (FTIR). It is suggested that appearance of typical fanlike or wheat bunchy crystals in urinary sediments under observation of lightmicroscope and determination by FTIR for the crystals are helpful in diagnosing sulfa calculi causced swine urolithiasis.

Nigerian Bread Contribute One Half of Recommended Vitamin a Intake in Poor-Urban Lagosian Preschoolers

Nigerian bread is baked with vitamin A fortified wheat flour. Study aimed at determining its contribution to preschoolers- vitamin A nutriture. A cross-sectional/experimental study was carried out in four poor-urban Local Government Areas (LGAs) of Metropolitan Lagos, Nigeria. A pretested food frequency questionnaire was administered to randomly selected mothers of 1600 preschoolers (24-59 months). Retinyl Palmitate content of fourteen bread samples randomly collected from bakeries in all LGAs was analyzed at 0 and 5 days at 25oC using High Performance Liquid Chromatography. Data analysis was done at p

Active Packaging Influence on Shelf Life Extension of Sliced Wheat Bread

The research object was wheat bread. Experiments were carried out at the Faculty of Food Technology of the Latvia University of Agriculture. An active packaging in combination with modified atmosphere (MAP, CO2 60% and N2 40%) was examined and compared with traditional packaging in air ambiance. Polymer Multibarrier 60, PP and OPP bags were used. Influence of iron based oxygen absorber in sachets of 100 cc obtained from Mitsubishi Gas Chemical Europe Ageless® was tested on the quality during the shelf of wheat bread. Samples of 40±4 g were packaged in polymer pouches (110 mm x 120 mm), hermetically sealed by MULTIVAC C300 vacuum chamber machine, and stored in room temperature +21.0±0.5 °C. The physiochemical properties – weight losses, moisture content, hardness, pH, colour, changes of atmosphere content (CO2 and O2) in headspace of packs, and microbial conditions were analysed before packaging and in the 7th, 14th, 21st and 28th days of storage.

Effect of Herbicides on Narrow Leaved Weeds and Yield of Wheat (Triticum aestivum L.)

This study was conducted to investigate the efficacy of five herbicides on narrow leaved weeds and growth and yield of wheat. An experiment was conducted at Agronomic Research Farm, University of Agriculture Faisalabad. The experiment was laid out in randomized complete block designee (RCBD) with three replications. Treatments studied were clodinafop (Topic-15 WG) at 37 g a.i. ha-1, clodinafop (Topaz-15 WG) at 45 g a.i. ha-1, fenoxaprop-p-ethyl (Puma Super-75 EW) at 45 g a.i. ha-1, fenoxaprop-p-ethyl (Gramicide-6.9 EW) at 85 g a.i. ha-1, fenoxaprop-p-ethyl (Chinlima-6.9 EW) at 85 g a.i. ha-1 and weedy check. Plots treated with fenoxaprop-p-ethyl (Puma Super-75 EW) at 45 g a.i. ha-1 produced relatively less weed biomass, more plant height, number of spike bearing tillers, number of grains per spike, 1000-grain weight and grain yield (4.20 t ha-1).

Biodegradation of PCP by the Rhizobacteria Isolated from Pentachlorophenol-tolerant Crop Species

Pentachlorophenol (PCP) is a polychlorinated aromatic compound that is widespread in industrial effluents and is considered to be a serious pollutant. Among the variety of industrial effluents encountered, effluents from tanning industry are very important and have a serious pollution potential. PCP is also formed unintentionally in effluents of paper and pulp industries. It is highly persistent in soils and is lethal to a wide variety of beneficial microorganisms and insects, human beings and animals. The natural processes that breakdown toxic chemicals in the environment have become the focus of much attention to develop safe and environmentfriendly deactivation technologies. Microbes and plants are among the most important biological agents that remove and degrade waste materials to enable their recycling in the environment. The present investigation was carried out with the aim of developing a microbial system for bioremediation of PCP polluted soils. A number of plant species were evaluated for their ability to tolerate different concentrations of pentachlorophenol (PCP) in the soil. The experiment was conducted for 30 days under pot culture conditions. The toxic effect of PCP on plants was studied by monitoring seed germination, plant growth and biomass. As the concentration of PCP was increased to 50 ppm, the inhibition of seed germination, plant growth and biomass was also increased. Although PCP had a negative effect on all plant species tested, maize and groundnut showed the maximum tolerance to PCP. Other tolerating crops included wheat, safflower, sunflower, and soybean. From the rhizosphere soil of the tolerant seedlings, as many as twenty seven PCP tolerant bacteria were isolated. From soybean, 8; sunflower, 3; safflower 8; maize 2; groundnut and wheat, 3 each isolates were made. They were screened for their PCP degradation potentials. HPLC analyses of PCP degradation revealed that the isolate MAZ-2 degraded PCP completely. The isolate MAZ-1 was the next best isolate with 90 per cent PCP degradation. These strains hold promise to be used in the bioremediation of PCP polluted soils.

Effect of Soil Tillage System upon the Soil Properties, Weed Control, Quality and Quantity Yield in Some Arable Crops

The paper presents the influence of the conventional ploughing tillage technology in comparison with the minimum tillage, upon the soil properties, weed control and yield in the case of maize (Zea mays L.), soya-bean (Glycine hispida L.) and winter wheat (Triticum aestivum L.) in a three years crop rotation. A research has been conducted at the University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, Romania. The use of minimum soil tillage systems within a three years rotation: maize, soya-bean, wheat favorites the rise of the aggregates hydro stability with 5.6-7.5% on a 0-20 cm depth and 5-11% on 20-30 cm depth. The minimum soil tillage systems – paraplow, chisel or rotary grape – are polyvalent alternatives for basic preparation, germination bed preparation and sowing, for fields and crops with moderate loose requirements being optimized technologies for: soil natural fertility activation and rationalization, reduction of erosion, increasing the accumulation capacity for water and realization of sowing in the optimal period. The soil tillage system influences the productivity elements of cultivated species and finally the productions thus obtained. Thus, related to conventional working system, the productions registered in minimum tillage working represented 89- 97% in maize, 103-112% in soya-bean, 93-99% in winter-wheat. The results of investigations showed that the yield is a conclusion soil tillage systems influence on soil properties, plant density assurance and on weed control. Under minimum tillage systems in the case of winter weat as an option for replacing classic ploughing, the best results in terms of quality indices were obtained from version worked with paraplow, followed by rotary harrow and chisel. At variants worked with paraplow were obtained quality indices close to those of the variant worked with plow, and protein and gluten content was even higher. At Ariesan variety, highest protein content, 12.50% and gluten, 28.6% was obtained for the variant paraplow.

The Effectiveness of Tebuconazole and Chitosan in Inhibiting the Growth of Fusarium Species on Winter Wheat Grain under Field Conditions

A three-year field experiment (2010-2012) was conducted to determine the abundance of epiphytic and endophytic filamentous fungi colonizing the grain of winter wheat cv. Bogatka. Wheat spikes were protected with tebuconazole or chitosan at the watery ripe stage. Untreated plants served as control. Tebuconazole exerted an inhibitory effect primarily on F. culmorum and F. graminearum, and its effectiveness was determined by the pressure from pathogens that infected wheat spikes during the growing season. Chitosan did not suppress the growth of Fusarium species and Alternaria alternata.

Wheat Yield Prediction through Agro Meteorological Indices for Ardebil District

Wheat prediction was carried out using different meteorological variables together with agro meteorological indices in Ardebil district for the years 2004-2005 & 2005–2006. On the basis of correlation coefficients, standard error of estimate as well as relative deviation of predicted yield from actual yield using different statistical models, the best subset of agro meteorological indices were selected including daily minimum temperature (Tmin), accumulated difference of maximum & minimum temperatures (TD), growing degree days (GDD), accumulated water vapor pressure deficit (VPD), sunshine hours (SH) & potential evapotranspiration (PET). Yield prediction was done two months in advance before harvesting time which was coincide with commencement of reproductive stage of wheat (5th of June). It revealed that in the final statistical models, 83% of wheat yield variability was accounted for variation in above agro meteorological indices.

The Effect of Complementary Irrigation in Different Growth Stages on Yield, Qualitative and Quantitative Indices of the Two Wheat (Triticum aestivum L.) Cultivars in Mazandaran

In most wheat growing moderate regions and especially in the north of Iran climate, is affected grain filling by several physical and abiotic stresses. In this region, grain filling often occurs when temperatures are increasing and moisture supply is decreasing. The experiment was designed in RCBD with split plot arrangements with four replications. Four irrigation treatments included (I0) no irrigation (check); (I1) one irrigation (50 mm) at heading stage; (I2) two irrigation (100 mm) at heading and anthesis stage; and (I3) three irrigation (150 mm) at heading, anthesis and early grain filling growth stage, two wheat cultivars (Milan and Shanghai) were cultured in the experiment. Totally raining was 453 mm during the growth season. The result indicated that biological yield, grain yield and harvest index were significantly affected by irrigation levels. I3 treatment produced more tillers number in m2, fertile tillers number in m2, harvest index and biological yield. Milan produced more tillers number in m2, fertile tillers in m2, while Shanghai produced heavier tillers and grain 1000 weight. Plant height was significant in wheat varieties while were not statistically significant in irrigation levels. Milan produced more grain yield, harvest index and biological yield. Grain yield shown that I1, I2, and I3 produced increasing of 5228 (21%), 5460 (27%) and 5670 (29%) kg ha-1, respectively. There was an interaction of irrigation and cultivar on grain yields. In the absence of the irrigation reduced grain 1000 weight from 45 to 40 g. No irrigation reduced soil moisture extraction during the grain filling stage. Current assimilation as a source of carbon for grain filling depends on the light intercepting viable green surfaces of the plant after anthesis that due to natural senescence and the effect of various stresses. At the same time the demand by the growing grain is increasing. It is concluded from research work that wheat crop irrigated Milan cultivar could increase the grain yield in comparison with Shanghai cultivar. Although, the grain yield of Shanghai under irrigation was slightly lower than Milan. This grain yield also was related to weather condition, sowing date, plant density and location conditions and management of fertilizers, because there was not significant difference in biological and straw yield. The best result was produced by I1 treatment. I2 and I3 treatments were not significantly difference with I1 treatment. Grain yield of I1 indicated that wheat is under soil moisture deficiency. Therefore, I1 irrigation was better than I0.

Polymorphism of HMW-GS in Collection of Wheat Genotypes

Processes of plant breeding, testing and licensing of new varieties, patent protection in seed production, relations in trade and protection of copyright are dependent on identification, differentiation and characterization of plant genotypes. Therefore, we focused our research on utilization of wheat storage proteins as genetic markers suitable not only for differentiation of individual genotypes, but also for identification and characterization of their considerable properties. We analyzed a collection of 102 genotypes of bread wheat (Triticum aestivum L.), 41 genotypes of spelt wheat (Triticum spelta L.), and 35 genotypes of durum wheat (Triticum durum Desf.), in this study. Our results show, that genotypes of bread wheat and durum wheat were homogenous and single line, but spelt wheat genotypes were heterogenous. We observed variability of HMW-GS composition according to environmental factors and level of breeding and predict technological quality on the basis of Glu-score calculation.

New Malate Dehydrogenase-Glutamate Oxaolacetate Aminotransferase Glutamate Oxaloacetate Aminotransferase Enzyme System from Cereals and its Bioengineering Application

Malate dehydrogenase-glutamate oxaloacetate aminotransferase (MDh-GOAT) enzyme complex (the EC) was isolated and purified from wheat and rise, their some main physicchemical properties were studied. Michael-s constants of the EC MDh-GOAT to malate, glutamate and NAD were investigated. This kinetic results show a high relationship to glutamate. Taking into account important role of the the EC in catabolism of glutamate – the central amino acid of a nitric exchange, there is a sharp necessity of deeper studying of this enzyme complex. Therefore the basic purpose of the work is studying the basic physical and chemical properties of this enzyme complex discovered by us, which would be very important for understanding the mechanisms of reaction catalyzed by the EC.

Efficiency of Floristic and Molecular Markers to Determine Diversity in Iranian Populations of T. boeoticum

In order to study floristic and molecular classification of common wild wheat (Triticum boeoticum Boiss.), an analysis was conducted on populations of the Triticum boeoticum collected from different regions of Iran. Considering all floristic compositions of habitats, six floristic groups (syntaxa) within the populations were identified. A high level of variation of T. boeoticum also detected using SSR markers. Our results showed that molecular method confirmed the grouping of floristic method. In other word, the results from our study indicate that floristic classification are still useful, efficient, and economic tools for characterizing the amount and distribution of genetic variation in natural populations of T. boeoticum. Nevertheless, molecular markers appear as useful and complementary techniques for identification and for evaluation of genetic diversity in studied populations.

Influence of Watertable Depth on Soil Sodicity and Salinity

In order to monitor the water table depth on soil profile salinity buildup, a field study was carried out during 2006-07. Wheat (Rabi) and Sorghum (Kharif) fodder were sown in with three treatments. The results showed that watertable depth lowered from 1.15m to 2.89 m depth at the end of experiment. With lower of watertable depth, pH, ECe and SAR decreased under crops both without and with gypsum and increased in fallowing. Soil moisture depletion was directly proportional to lowering of watertable. With the application of irrigation water (58cm) pH, ECe and SAR were reduced in cropped plots, reduction was higher in gypsum applied plots than non-gypsum plots. In case of fallowing, there was increase in pH, EC, while slight reduction occurred in SAR values. However, soil salinity showed an increasing upward trend under fallowing and its value in 0-30 cm soil layer was the highest amongst the treatments.

Production of WGHs and AFPHs using Protease Combinations at High and Ambient Pressure

Wheat gluten hydrolyzates (WGHs) and anchovy fine powder hydrolyzates (AFPHs) were produced at 300 MPa using combinations of Flavourzyme 500MG (F), Alcalase 2.4L (A), Marugoto E (M) and Protamex (P), and then were compared to those produced at ambient pressure concerning the contents of soluble solid (SS), soluble nitrogen and electrophoretic profiles. The contents of SS in the WGHs and AFPHs increased up to 87.2% according to the increase in enzyme number both at high and ambient pressure. Based on SS content, the optimum enzyme combinations for one-, two-, three- and four-enzyme hydrolysis were determined as F, FA, FAM and FAMP, respectively. Similar trends were found for the contents of total soluble nitrogen (TSN) and TCA-soluble nitrogen (TCASN). The contents of SS, TSN and TCASN in the hydrolyzates together with electrophoretic mobility maps indicates that the high-pressure treatment of this study accelerated protein hydrolysis compared to ambient-pressure treatment.

A Tool for Creation Artificial Symbiotic Associations of Wheat

This paper reports optimization of characteristics of bioballistic transformation of spring soft wheat (Triticum aestivum L. cultivar Raduga) and getting of transgenic plants, carrying pea lectin gene. This gene will let to create new associative wheat symbiosis with nodule bacteria of field pea, which has growth encouraging, fungistatic and other useful characteristics.

Use of GIS for the Performance Evaluation of Canal Irrigation System in Rice Wheat Cropping Zone

The research study evaluated the performance of irrigation system by using special scientific tools like Remote Sensing and GIS technology, so that proper measurements could be taken for the sustainable agriculture and water management. Different performance evaluation parameters had been calculated for the purposed data was gathered from field investigation and different government and private organizations. According to the calculations, organic matter ranges from 0.19% (low value) to 0.76% (high value). In flat irrigation system for wheat yield ranges from 3347.16 to 5260.39 kg/ha, while the total water applied to wheat crop ranges from 252.94 to 279.19 mm and WUE ranges from 13.07 to 18.37 kg/ha/mm. For rice yield ranges from 3347.47 to 5433.07 kg/ha with total water supplied to rice crop ranges from 764.71 to 978.15 mm and WUE ranges from 3.49 to 5.71 kg/ha/mm. Similarly, in raised bed system wheat yield ranges from 4569.13 to 6008.60 kg/ha, total water supplied ranges from 158.87 to 185.09 mm and WUE ranges from 27.20 to 33.54 kg/ha/mm while in rice crop, yield ranges from 5285.04 to 6716.69 kg/ha, total water supplied ranges from 600.72 to 755.06 mm and WUE ranges from 6.41 to 10.05 kg/ha/mm. Almost 51.3% water saving is observed in bed irrigation system as compared to flat system. Less water supplied to beds is more affective as its WUE value is higher than flat system where more water is supplied in both the seasons. Similarly, RWS values show that maximum water deficit while minimum area is getting adequate water supply. Greater yield is recorded in bed system as plant per square meter is more in bed system in comparison of flat system Thus, the integration of GIS tools to regularly compute performance indices could provide irrigation managers with the means for managing efficiently the irrigation system.

Comparative Approach of Measuring Price Risk on Romanian and International Wheat Market

This paper aims to present the main instruments used in the economic literature for measuring the price risk, pointing out on the advantages brought by the conditional variance in this respect. The theoretical approach will be exemplified by elaborating an EGARCH model for the price returns of wheat, both on Romanian and on international market. To our knowledge, no previous empirical research, either on price risk measurement for the Romanian markets or studies that use the ARIMA-EGARCH methodology, have been conducted. After estimating the corresponding models, the paper will compare the estimated conditional variance on the two markets.