Abstract: In vitro gastro-duodenal digestion model was used to investigate the changes of emulsions under digestion conditions. Oil in water emulsions stabilized by whey proteins (2%) and stabilized by whey proteins (2%) with addition of carboxymethyl cellulose (0.75%) as gelling agent of continuous phase were prepared at pH7. Both emulsions were destabilized under gastric conditions; however the protective role of carboxymethyl cellulose was indicated by recording delay of fat digestibility of this emulsion. In the presence of carboxymethyl cellulose whey proteins on the interfacial surface of droplets were more resistant to gastric degradation causing limited hydrolysis of fat due to the poor acceptability of lipids for the enzymes. Studies of emulsions using in vivo model supported results from in vitro studies. Lower content of triglycerides in blood serum and higher amount of fecal fat of rats were determined when rats were fed by diet containing emulsion made with whey proteins and carboxymethyl cellulose.
Abstract: Mutations of the telomeric copy of the survival motor neuron 1 (SMN1) gene cause spinal muscular atrophy. A deletion of the Eef1a2 gene leads to lower motor neuron degeneration in wasted mice. Indirect evidences have been shown that the eEF1A protein family may interact with SMN, and our previous study showed that abnormalities of neuromuscular junctions in wasted mice were similar to those of Smn mutant mice. To determine potential colocalization between SMN and tissue-specific translation elongation factor 1A2 (eEF1A2), an immunochemical analysis of HeLa cells transfected with the plasmid pcDNA3.1(+)C-hEEF1A2- myc and a new quantitative test of colocalization by intensity correlation analysis (ICA) was used to explore the association of SMN and eEF1A2. Here the results showed that eEF1A2 redistributed from the cytoplasm to the nucleus in response to serum and epidermal growth factor. In the cytoplasm, compelling evidence showed that staining for myc-tagged eEF1A2 varied in synchrony with that for SMN, consistent with the formation of a SMN-eEF1A2 complex in the cytoplasm of HeLa cells. These findings suggest that eEF1A2 may colocalize with SMN in the cytoplasm and may be a component of the SMN complex. However, the limitation of the ICA method is an inability to resolve colocalization in components of small organelles such as the nucleus.
Abstract: The effects of commercial or bovine yeasts on the
performance and blood variables of broiler chickens intoxicated with
aflatoxin were investigated in broilers. Four hundred eighty broilers
(Arbor Acres; 3-wk-old) were randomly assigned to 4 groups. Each
group (120 broiler chickens) was further randomly divided into 6
replicates of 20 chickens. The treatments were control diet without
additives (treatment 1), 250 ppb AFB1 (treatment 2), commercial
yeast, Saccharomyces cerevisiae, (CY 2.5 x 107 CFU/g) + 250 ppb
AFB1 (treatment 3) and bovine yeast, Saccharomyces cerevisiae,
(BY 2.5 x 107 CFU/g + 250 ppb AFB1 (treatment 4). Complete
randomized design (CRD) was used in the experiment. Feed
consumption and body weight were recorded at every five-day
period. On day 42, carcass compositions were determined from 30
birds per treatment. While chicks were sacrificed, 3-4 ml blood
sample was taken and stored frozen at (-20°C) for serum chemical
analysis to determine effects of consumption of diets on blood
chemistry (total protein, albumin, glucose, urea, cholesterol and
triglycerides). There were no significant differences in ADFI among
the treatments(P>0.05). However, BWG, FCR and mortality were
highly significantly different (P
Abstract: In Indonesia, goat milk is often consumed and
believed as anti-allergy. The objective of this research was to study
the effect of goat milk and their fractions (casein and whey)
supplementation on total serum IgE concentrations and leukocytes
count in rat sensitized with contact allergen dinitrochlorobenzene
(DNCB). Female Wistar rats 6-8 weeks old were divided into four
groups: 1) whey, 2) casein, 3) whole milk supplementation and 4)
phosphate-buffered saline/PBS (control). The results showed that
supplementation of goat milk on rats did not affects on total serum
IgE concentrations and number of leukocytes. After sensitized with
DNCB, the monocyte percentage in rats was higher (P
Abstract: The possible therapeutic effect of cannabidiol, the major non-psychotropic Cannabis constituent, was investigated against acute hepatotoxicity induced by a single oral dose of acetaminophen (500mg/kg) in mice. Cannabidiol (two intraperitoneal injections, 5mg/kg, each) was given 1 hour and 12 hours following acetaminophen administration. Acetaminophen administration caused significant elevations of serum alanine aminotransferase, and hepatic malondialdehyde, and nitric oxide levels, and a significant decrease in hepatic reduced glutathione. Cannabidiol significantly attenuated the deterioration in the measured biochemical parameters resulted from acetaminophen administration. Also, histopathological examination showed that cannabidiol markedly attenuated ameliorated acetaminophen-induced liver tissue damage. These results emphasize that cannabidiol represents a potential therapeutic option to protect against acetaminophen hepartotoxicity which is a common clinical problem.
Abstract: Mushrooms are a group of fleshy macroscopic fungi.
They have been valued throughout the world as both edible and
medicine. They are highly nutritious with good amount of quality
proteins, vitamins and minerals. An edible mushroom, Calocybe
indica was selected to validate its nutritional and medicinal
properties. Since tissue damage in hyperglycemia has been related to
oxidative stress, we evaluated the enzymatic and non-enzymatic
antioxidant status in the serum, liver and kidney since they are the
target organs in diabetic complications. From the results, increased
oxidative stress and decreased antioxidants might be related to the
causation of diabetes mellitus. The treatment in the diabetic rats with
the Calocybe indica showed an increase in the antioxidant system
and decrease in the production of free radicals. The mushrooms
which contain antioxidant phytochemicals has potential free radical
scavenging capacity and hence can induce the antioxidant system in
the body significantly reduces the generated free radicals thereby
maintaining the normal levels of the antioxidants
Abstract: Effect of oral administration of “Gadagi" tea on liver
function was assessed on 50 healthy male albino rats which were
grouped and administered with different doses(mg/kg) i.e low dose
(380mg/kg, 415mg/kg, 365mg/kg, 315mg/kg for “sak", “sada" and
“magani" respectively), standard dose ( 760mg/kg, 830mg/kg,
730mg/kg for “sak-, “sada" and “magani" respectively) and high dose
(1500mg/kg, 1700mg/kg and 1460mg/kg for “sak--,"sada" and
“magani" groups respectively) for a period of four weeks. Animals
that were not administered with the tea constituted the control group.
At the end of fourth week, the animals were sacrificed and their
serum alanine aminotransferase (ALT), aspartate aminotransferase
(AST), alkaline phosphatase (ALP), total protein (TP), albumin
(ALB), and globulins (GLO) were determined. Mean serum ALT and
ALP activities were significantly higher (P
Abstract: Sensitive and predictive DILI (Drug Induced Liver
Injury) biomarkers are needed in drug R&D to improve early
detection of hepatotoxicity. The discovery of DILI biomarkers that
demonstrate the predictive power to identify individuals at risk to
DILI would represent a major advance in the development of
personalized healthcare approaches. In this healthy volunteer
acetaminophen study (4g/day for 7 days, with 3 monitored nontreatment
days before and 4 after), 450 serum samples from 32
subjects were analyzed using protein profiling by antibody
suspension bead arrays. Multiparallel protein profiles were generated
using a DILI target protein array with 300 antibodies, where the
antibodies were selected based on previous literature findings of
putative DILI biomarkers and a screening process using pre dose
samples from the same cohort. Of the 32 subjects, 16 were found to
develop an elevated ALT value (2Xbaseline, responders). Using the
plasma profiling approach together with multivariate statistical
analysis some novel findings linked to lipid metabolism were found
and more important, endogenous protein profiles in baseline samples
(prior to treatment) with predictive power for ALT elevations were
identified.
Abstract: This experiment was conducted to investigate the
effect of dietary supplementation of different levels of black seed
(Nigella sativa L.) on the performance and immune response of broiler chicks. A total 240 day-old broiler chicks were used and
randomly allotted equally into six experimental groups designated as 1, 2, 3, 4, 5 and 6 having black seed at the rate of 0, 2, 4, 6, 8 and
10 g /kg diet respectively. The study was lasted for 42 days. Average body weight, weight gain, relative growth rate, feed
conversion, antibody titer against Newcastle disease, phagocytic activity and phagocytic index, some blood parameters(GOT, GPT,
Glucose, Cholesterol, Triglyceride, Total protein, Albumen, WBCs,
RBCs, Hb and PCV), dressing percentage, weight of different body
organs, abdominal fat weight, were determined. It was found that, N. Sativa significantly improved final body weight, total body gain
and feed conversion ratio of groups 2 and 3 when compared with the control group. Higher levels of N. Sativa did not improve
growth performance of the chicks. Non significant differences were
observed for antibody titer against Newcastle virus, WBCs count,
serum GOT, glucose level, dressing %, relative liver, spleen, heart and head percentages. Lymphoid organs (Bursa and Thymus)
improved significantly with increasing N. Sativa level in all supplemented groups. Serum cholesterol, triglyceride and visible fat
% significantly decreased with Nigella sativa supplementation while
serum GPT level significantly increased with nigella sativa
supplementation.
Abstract: Bacterial magnetic nanoparticles have great useful potential in biotechnological and biomedical applications. In this study, a liquid growth medium was modified for cultivation a fastidious magnetotactic bacterium that has been isolated from Anzali
lagoon, Iran in our previous research. These modifications include
change in vitamin, mineral, carbon sources and etcetera. In our
experience, the serum bottles and designed air-tight laboratory bottles
were used to create microaerobic conditions in order to development
of a method for scale-up experiment. This information may serve as a
guide to green chemistry based biological protocols for the synthesis
of magnetic nanoparticles with control over the chemical
composition, morphology and size.
Abstract: Expression and secretion of inflammation markers are
disturbed in obesity. Interleukin-6 reduces body fat mass. The
common G-174C polymorphism in the promoter of IL-6 gene has
been reported that effects on transcriptional regulation. The objective
was to investigate association of the common polymorphism G-174C
with obesity in Iranian population. The present study is cross
sectional association study that included 242 individuals (110 men
and 132 women). Serum IL-6 levels, C-reactive protein, fasting
blood glucose and blood lipids profile were measured .BMI and
WHR were calculated. Genotyping is carried out by PCR and RFLP.
The frequencies of G and C allele were 64.5% and 35.5%,
respectively. The G-174C polymorphism was not associated with
BMI and WHR. However in obese individual, fasting blood glucose
was significantly higher in carrier of C allele compared with the noncarrier.
The IL-6 G-174C polymorphism is not a risk factor for
obesity in Iranian population.
Abstract: Aim of this work was to compare the efficacy of two
loading methods of proteins onto polymeric nanocarriers: adsorption
and encapsulation methods. Preliminary studies of protein loading
were done using Bovine Serum Albumin (BSA) as model protein.
Nanocarriers were prepared starting from polylactic co-glycolic acid
(PLGA) polymer; production methods used are two different variants
of emulsion evaporation method. Nanoparticles obtained were
analyzed in terms of dimensions by Dynamic Light Scattering and
Loading Efficiency of BSA by Bradford Assay. Loaded
nanoparticles were then submitted to in-vitro protein dissolution test
in order to study the effect of the delivery system on the release rate
of the protein.
Abstract: Glomerular filtration rate (GFR) is a measure of
kidney function. It is usually estimated from serum concentrations of
cystatin C or creatinine although there has been considerable debate
in the literature about (i) the best equation to use and (ii) the
variability in the correlation between the concentrations of creatinine
and cystatin C. The equations for GFR can be written in a general
form and from these I calculate the error of the GFR estimates
associated with analyte measurement error. These show that the
error of the GFR estimates is such that it is not possible to distinguish
between the equations over much of the concentration range of either
analyte. The general forms of the equations are also used to derive
an expression for the concentration of cystatin C as a function of the
concentration of creatinine. This equation shows that these analyte
concentrations are not linearly related. Clinical reports of cystatin C
and creatinine concentration are consistent with the expression
derived.
Abstract: We investigated oxidative DNA damage caused by
radio frequency radiation using 8-oxo-7, 8-dihydro-2'-
deoxyguanosine (8-oxodG) generated in mice tissues after exposure
to 900 MHz mobile phone radio frequency in three independent
experiments. The RF was generated by a Global System for Mobile
Communication (GSM) signal generator. The radio frequency field
was adjusted to 25 V/m. The whole body specific absorption rate
(SAR) was 1.0 W/kg. Animals were exposed to this field for 30 min
daily for 30 days. 24 h post-exposure, blood serum, brain and spleen
were removed and DNA was isolated. Enzyme-linked
immunosorbent assay (ELISA) was used to measure 8-oxodG
concentration. All animals survived the whole experimental period.
The body weight of animals did not change significantly at the end of
the experiment. No statistically significant differences observed in
the levels of oxidative stress. Our results are not in favor of the
hypothesis that 900 MHz RF induces oxidative damage.
Abstract: This study has investigated the antidiabetic and
antioxidant potential of Pseudovaria macrophylla bark extract on
streptozotocin–nicotinamide induced type 2 diabetic rats. LCMSQTOF
and NMR experiments were done to determine the chemical
composition in the methanolic bark extract. For in vivo experiments,
the STZ (60 mg/kg/b.w, 15 min after 120 mg/kg/1 nicotinamide, i.p.)
induced diabetic rats were treated with methanolic extract of
Pseuduvaria macrophylla (200 and 400 mg/kg·bw) and
glibenclamide (2.5 mg/kg) as positive control respectively.
Biochemical parameters were assayed in the blood samples of all
groups of rats. The pro-inflammatory cytokines, antioxidant status
and plasma transforming growth factor βeta-1 (TGF-β1) were
evaluated. The histological study of the pancreas was examined and
its expression level of insulin was observed by
immunohistochemistry. In addition, the expression of glucose
transporters (GLUT 1, 2 and 4) were assessed in pancreas tissue by
western blot analysis. The outcomes of the study displayed that the
bark methanol extract of Pseuduvaria macrophylla has potentially
normalized the elevated blood glucose levels and improved serum
insulin and C-peptide levels with significant increase in the
antioxidant enzyme, reduced glutathione (GSH) and decrease in the
level of lipid peroxidation (LPO). Additionally, the extract has
markedly decreased the levels of serum pro-inflammatory cytokines
and transforming growth factor beta-1 (TGF-β1). Histopathology
analysis demonstrated that Pseuduvaria macrophylla has the
potential to protect the pancreas of diabetic rats against peroxidation
damage by downregulating oxidative stress and elevated
hyperglycaemia. Furthermore, the expression of insulin protein,
GLUT-1, GLUT-2 and GLUT-4 in pancreatic cells was enhanced.
The findings of this study support the anti-diabetic claims of
Pseudovaria macrophylla bark.
Abstract: The objective of the present study was to examine the
dose-response relationships between antioxidant parameters and liver
contaminant levels of Kazakhstan light crude oil (KLCO) in albino
rats. The animals were repeatedly exposed, by intraperitoneal
injection, to low dosages (0.5–1.5 ml/kg) of KLCO. Rats exposed to
these doses levels did not show any apparent symptoms of
intoxication. Serum aminotransferases increased significantly
(p
Abstract: We investigated the response of testosterone (T),
growth hormone (GH), cortisol (C), steroid hormone binding
globulin (SHBG), insulin-like growth factor (IGF-1), insulin-like
growth factor binding protein-3 (IGFBP-3), and some anaboliccatabolic
indexes, i.e.: T/C, T/SHBG, and IGF-1/IGFBP-3 to
maximal exercise in endurance-trained athletes (TREN) and
untrained subjects (CG). The baseline concentration of IGF-1 was
higher in athletes (TREN) when compared to the CG (p
Abstract: The alterations in pancreas gland secretion hormones
following an aerobic and exhausting exercise was the purpose of this
study. Sixteen healthy men participated in the study. The blood
samples of these participants were taken in four stages under fasting
condition. The first sample was taken before Bruce exhausting and
aerobic test, the second sample was taken after Bruce exercise and
the third and forth stages samples were taken 24 and 48 hours after
the exercises respectively. The final results indicated that a strenuous
aerobic exercise can have a significant effect on glucagon and insulin
concentration of blood serum. The increase in blood serum insulin
was higher after 24 and 48 hours. It seems that an intensive exercise
has little effect on changes in glucagon concentration of blood serum.
Also, disorder in secretion in glucagon and insulin concentration of
serum disturbs athletes- exercise.
Abstract: The main objective of this study was to demonstrate that differentiation of infected and vaccinated animals (DIVA) strategy using different ELISA tests is possible when a subunit vaccine (Haemagglutinin protein) is used to prevent Avian influenza. Special emphasis was placed on the differentiation in the serological response to different components of the AIV (Nucleoprotein, Neuraminidase, Haemagglutinin, Nucleocapsid) between chickens that were vaccinated with a whole virus kill vaccine and recombinant vaccine. Furthermore, the potential use of this DIVA strategy using ELISA assays to detect Neuraminidase 1 (N1) was analyzed as strategy in countries where the field virus is H5N1 and the vaccine used is formulated with H5N2. Detection of AIV-s antibodies to any component in serum was negative for all animals on the study days 0-13. At study day 14 the titers of antibodies against Nucleoprotein (NP) and Nucleocapsid (NC) rose in the experimental groups vaccinated with Volvac® AI KV and were negatives during all the trial in the experimental groups vaccinated with a subunit H5; significant statistically differences were observed between these groups (p < 0.05). The seroconversion either Haemagglutinin or Neuraminidase was evident after 21 days post-vaccination in the experimental groups vaccinated with the respective viral fraction. Regarding the main aim of this study and according with the results that were obtained, use a combination of different ELISA test as a DIVA strategy is feasible when the vaccination is carry out with a subunit H5 vaccine. Also is possible to use the ELISA kit to detect Neuraminidase (either N1 or N2) as a DIVA concept in countries where H5N1 is present and the vaccination programs are done with H5N2 vaccine.
Abstract: Oxyleotris marmorata is considered as
undomesticated fish, and its culture occasionally faces a problem of
food deprivation. The present study aims to evaluate alteration of
hematological indices, blood chemical associated with liver function
during 4 weeks of fasting. A non-linear relationships between fasting
days and hematological parameters (red blood cell number; y = -
0.002x2 + 0.041x + 1.249; R2=0.915, P0.05), mean corpuscular
volume; y = -0.180x2 + 2.183x + 149.61; R2=0.732, P>0.05, mean
corpuscular hemoglobin; y = -0.041x2 + 0.862x + 29.864; R2=0.818,
P>0.05 and mean corpuscular hemoglobin concentration; y = -
0.044x2 + 0.711x + 21.580; R2=0.730, P>0.05) were demonstrated.
Significant change in hematocrit (Ht) during fasting period was
observed. Ht elevated sharply increase at the first weeks of fasting
period. Higher Ht also was detected during week 2-4 of fasting time.
The significant reduction of hepatosomatic index was observed (y = -
0.007x2 - 0.096x + 1.414; R2=0.968, P0.05, serum glutamic oxaloacetic transaminase;
y = 0.005x2 – 0.201x2 + 1.297x + 33.256; R2=1, P0.05). Taken together, prolonged fasting has
deleterious effects on hematological indices, liver mass and enzyme
associated in liver function. The marked adverse effects occurred
after the first week of fasting state.