Effect of Oral Administration of “Gadagi“ Tea on Liver Function in Rats
Effect of oral administration of “Gadagi" tea on liver
function was assessed on 50 healthy male albino rats which were
grouped and administered with different doses(mg/kg) i.e low dose
(380mg/kg, 415mg/kg, 365mg/kg, 315mg/kg for “sak", “sada" and
“magani" respectively), standard dose ( 760mg/kg, 830mg/kg,
730mg/kg for “sak-, “sada" and “magani" respectively) and high dose
(1500mg/kg, 1700mg/kg and 1460mg/kg for “sak--,"sada" and
“magani" groups respectively) for a period of four weeks. Animals
that were not administered with the tea constituted the control group.
At the end of fourth week, the animals were sacrificed and their
serum alanine aminotransferase (ALT), aspartate aminotransferase
(AST), alkaline phosphatase (ALP), total protein (TP), albumin
(ALB), and globulins (GLO) were determined. Mean serum ALT and
ALP activities were significantly higher (P<0.05) in rats orally
administered with high dose of “sak" and those administered with
standard dose of “sada" than those of the control group, suggesting a
probable impairment of liver function due to liver cytolysis.Mean
serum AST, ALT and ALP activities were significantly lower
(P<0.05) in rats that were orally administered with high dose of
“magani" than that of the control group, suggesting a probable
improvement in liver function (due to decrease in liver cytolysis).
Mean serum TP, ALB and GLO levels were significantly higher
(P<0.05) in rats that were orally administered with the various doses
of“sak", “sada" and “magani" than those of the control group. This
also suggests a probable improvement in the synthetic function of the
liver.Thus, some dosages of “sak" and “sada could be hepatotoxic,
whereas “magani" especially at the high dose administered could
have pharmacologically positive effect on the liver of the rats.
[1] Kent, M., Vande, G. and Struart, I.F.(1999). Concepts of Human
Anatomy and Physiology. Fifth edition. D.Van Nostrand Company, New
York. Pp 838-845.
[2] Gadanya, A.M. (2011). Biochemical and Toxicological studies on
"Gadagi"tea in rats. Ph.D. Thesis Department of Biochemistry, Bayero
University Kano, P2.
[3] Reitman, S. and Frankel, S. (1957). Colorimetric method for the
determination of serum glutamic oxaloacetate and glutamic pyruvic
transaminases. Amer. J. Clin. Pathol, 28; 56-61.
[4] King, E.J. and Armstrong, A.R. (1964). Determination of serum alkaline
phosphatase activity. Canadian Medical Journal 31:376-377.
[5] Gornall, A.G., Bardawill, C.J. and David, M.M. (1949). Determination
of serum proteins by means of buiret reaction. Journal of Biological
Chemistry. 28: 177 - 175.
[6] Jandrassik, L. and Grof, P. (1938). Colorimetric Method for the
Determination Srum Bilirubin. Clinial Biochemistry.297:81.
[7] Doumas, B.T., Waston, W.A. and Briggs, M.A. (1971). Albumin
standards and the measurement of serum albumin with bromocresol
green. Clinical Chemical Acta, 31: 87-89.
[8] Wada, H. and Snell, E.E. (1962). Enzymatic transmination of
pyridoxamine - pyruvate transaminase. J. Biol. Chem. 237: 133 - 137
[9] Gadanya, A.M.,Sule, M.S. and Atiku, M.K. (2011). Analysis of some
Phytochemicals in "Gadagi" tea commonly consumed in Kano,
Nigeria.(Unpublished manuscript).
[10] Obi, F.O. and Uneh, E. (2003). pH department prevention of carbon
tetrachloride-induced lipoperoxidation in rats by ethanolic extract of
Hibiscus rosasinensis petal. Biokeemstri 13:42-50.
[11] Atiku, M.K., Adamu, D.J.M., Gadanya, A.M. and Shehu, M.A. (2009).
The effect of "Gadagi" tea on liver function and serum glucose
concentration in Albino rats. Bayero Journal of Pure and Applied
Sciences 2 (1):125-127.
[1] Kent, M., Vande, G. and Struart, I.F.(1999). Concepts of Human
Anatomy and Physiology. Fifth edition. D.Van Nostrand Company, New
York. Pp 838-845.
[2] Gadanya, A.M. (2011). Biochemical and Toxicological studies on
"Gadagi"tea in rats. Ph.D. Thesis Department of Biochemistry, Bayero
University Kano, P2.
[3] Reitman, S. and Frankel, S. (1957). Colorimetric method for the
determination of serum glutamic oxaloacetate and glutamic pyruvic
transaminases. Amer. J. Clin. Pathol, 28; 56-61.
[4] King, E.J. and Armstrong, A.R. (1964). Determination of serum alkaline
phosphatase activity. Canadian Medical Journal 31:376-377.
[5] Gornall, A.G., Bardawill, C.J. and David, M.M. (1949). Determination
of serum proteins by means of buiret reaction. Journal of Biological
Chemistry. 28: 177 - 175.
[6] Jandrassik, L. and Grof, P. (1938). Colorimetric Method for the
Determination Srum Bilirubin. Clinial Biochemistry.297:81.
[7] Doumas, B.T., Waston, W.A. and Briggs, M.A. (1971). Albumin
standards and the measurement of serum albumin with bromocresol
green. Clinical Chemical Acta, 31: 87-89.
[8] Wada, H. and Snell, E.E. (1962). Enzymatic transmination of
pyridoxamine - pyruvate transaminase. J. Biol. Chem. 237: 133 - 137
[9] Gadanya, A.M.,Sule, M.S. and Atiku, M.K. (2011). Analysis of some
Phytochemicals in "Gadagi" tea commonly consumed in Kano,
Nigeria.(Unpublished manuscript).
[10] Obi, F.O. and Uneh, E. (2003). pH department prevention of carbon
tetrachloride-induced lipoperoxidation in rats by ethanolic extract of
Hibiscus rosasinensis petal. Biokeemstri 13:42-50.
[11] Atiku, M.K., Adamu, D.J.M., Gadanya, A.M. and Shehu, M.A. (2009).
The effect of "Gadagi" tea on liver function and serum glucose
concentration in Albino rats. Bayero Journal of Pure and Applied
Sciences 2 (1):125-127.
@article{"International Journal of Biological, Life and Agricultural Sciences:59239", author = "A. M. Gadanya and M. S. Sule and M. K. Atiku", title = "Effect of Oral Administration of “Gadagi“ Tea on Liver Function in Rats", abstract = "Effect of oral administration of “Gadagi" tea on liver
function was assessed on 50 healthy male albino rats which were
grouped and administered with different doses(mg/kg) i.e low dose
(380mg/kg, 415mg/kg, 365mg/kg, 315mg/kg for “sak", “sada" and
“magani" respectively), standard dose ( 760mg/kg, 830mg/kg,
730mg/kg for “sak-, “sada" and “magani" respectively) and high dose
(1500mg/kg, 1700mg/kg and 1460mg/kg for “sak--,"sada" and
“magani" groups respectively) for a period of four weeks. Animals
that were not administered with the tea constituted the control group.
At the end of fourth week, the animals were sacrificed and their
serum alanine aminotransferase (ALT), aspartate aminotransferase
(AST), alkaline phosphatase (ALP), total protein (TP), albumin
(ALB), and globulins (GLO) were determined. Mean serum ALT and
ALP activities were significantly higher (P", keywords = "Gadagi" tea, Liver function, Oral, Rats.", volume = "7", number = "1", pages = "50-4", }