Abstract: Polarization modulation infrared reflection absorption
spectroscopy (PM-IRRAS) in combination with electrochemistry,
was employed to study the influence of surface charge (potential) on
the kinetics of bovine serum albumin (BSA) adsorption on a
biomedical-grade 316LVM stainless steel surface is discussed. The
BSA adsorption kinetics was found to greatly depend on the surface
potential. With an increase in surface potential towards more
negative values, both the BSA initial adsorption rate and the
equilibrium (saturated) surface concentration also increased. Both
effects were explained on the basis of replacement of well-ordered
water molecules at the 316LVM / solution interface, i.e. by the
increase in entropy of the system.
Abstract: The objective of the present study was to evaluate the
potential of hollow microneedles for enhancing the transdermal
delivery of Bovine Serum Albumin (MW~66,000 Da)-Fluorescein
Isothiocyanate (BSA-FITC) conjugate, a hydrophilic large molecular
compound. Moreover, the effect of different formulations was
evaluated. The series of binary mixtures composed of propylene
glycol (PG) and pH 7.4 phosphate buffer solution (PBS) was
prepared and used as a medium for BSA-FITC. The results showed
that there was no permeation of BSA-FITC solution across the
neonatal porcine skin without using hollow microneedles, whereas
the cumulative amount of BSA-FITC released at 8 h through the
neonatal porcine skin was about 60-70% when using hollow
microneedles. Furthermore, the results demonstrated that the higher
volume of PG in binary mixtures injected, the lower cumulative
amount of BSA-FITC released and release rate of BSA-FITC from
skin. These release profiles of BSA-FITC in binary mixtures were
expressed by Fick-s law of diffusion. These results suggest the
utilization of hollow microneedle to enhance transdermal delivery of
protein and provide useful information for designing an effective
hollow microneedle system.
Abstract: Hepatocellular carcinoma, also called hepatoma, most
commonly appears in a patient with chronic viral hepatitis. In
patients with a higher suspicion of HCC, such as small or subtle
rising of serum enzymes levels, the best method of diagnosis
involves a CT scan of the abdomen, but only at high cost. The aim of
this study was to increase the ability of the physician to early detect
HCC, using a probabilistic neural network-based approach, in order
to save time and hospital resources.
Abstract: A major challenge in camel productivity is the high
mortality rate of camel calves in the early stage due to the lack of
colostrums. This study investigates the time required for the calves to
obtain the optimum amount of the immunoglobulin (IgG). Eleven
pregnant female camels (Camelus Dromedarus) were selected
randomly and variant in age and gestation. After delivery, 7 calves
were obtained and used for this investigation. Colostrum samples
were collected from mothers immediately after parturition. Blood
samples were obtained from the calves as follow: 0 day (before
suckling), 24, 48, 72, 96, 120 and 144 hours, 2nd, 3rd, and 4th weeks
post suckling. Blood serum and colostrums whey were separated and
used to determine IgG concentration, total protein and concentration
of Cortisol and Thyroxin. The results showed high levels of IgG in
camel colostrums (328.8 ± 4.5 mg / ml). The IgG concentration in
serum of calves was the highest within 1st 24 h after suckling (140.75
mg /ml), and then declined gradually reached lower level at 144 h
(41.97 mg / ml). The average turnover rate (t 1/2) of serum IgG in
the all cases was 3.22 days. The turnover of ranged from 2.56 days
for calves have values of IgG more than average and 7.7 days for
those with values below average. In spite of very high levels of
thyroxin in sera of new born the results showed no correlation
between cortisol and thyroxin with IgG levels.
Abstract: Biochemical investigations were carried out to assess
the effect of different exposure regimes of Kazakhstan crude oil
(KCO) on hepatic antioxidant defense system in albino rats.
Contaminants were delivered under two different dosing regimes,
with all treatments receiving the same total contaminant load by the
end of the exposure period. Rats in regime A injected with KCO
once at a dose of 6 ml/kg bw while in regime B injected multiply at a
dose of 1.5 ml/kg bw on day 1, 3, 5 and 8. Antioxidant biomarkers
were measured in hepatic tissue after 1, 3, 5 and 8 days. Significant
induction was observed in serum aminotransferases (ALT, AST)
(p
Abstract: Delayed wound healing in diabetes is primarily
associated with hyperglycemia, over-expression of inflammatory
marker, oxidative stress and delayed collagen synthesis. This
unmanaged wound is producing high economic burden on the
society. Thus research is required to develop new and effective
treatment strategies to deal with this emerging issue. Our present
study incorporates the evaluation of wound healing effects of 50%
ethanol extract of Ocimum sanctum (OSE) in streptozotocin
(45mg/kg)-induced diabetic rats with concurrent wound ulcer. The
animals showing diabetes (Blood glucose level >140 and
Abstract: Non-viral gene carriers composed of biodegradable
polymers or lipids have been considered as a safer alternative for gene
carriers over viral vectors. We have developed multi-functional
nano-micelles for both drug and gene delivery application.
Polyethyleneimine (PEI) was modified by grafting stearic acid (SA)
and formulated to polymeric micelles (PEI-SA) with positive surface
charge for gene and drug delivery. Our results showed that PEI-SA
micelles provided high siRNA binding efficiency. In addition, siRNA
delivered by PEI-SA carriers also demonstrated significantly high
cellular uptake even in the presence of serum proteins. The
post-transcriptional gene silencing efficiency was greatly improved by
the polyplex formulated by 10k PEI-SA/siRNA. The amphiphilic
structure of PEI-SA micelles provided advantages for multifunctional
tasks; where the hydrophilic shell modified with cationic charges can
electrostatically interact with DNA or siRNA, and the hydrophobic
core can serve as payloads for hydrophobic drugs, making it a
promising multifunctional vehicle for both genetic and chemotherapy
application.
Abstract: Obesity is frequent attendant phenomenon of patients
with endocrinological disease. Between BMI and endocrinological
diseases is close correlation. In thesis we focused on the allocation of
hormone concentration – PTH and TSH, CHOL a mineral element Ca
in a blood serum. The examined group was formed by 100
respondents (women) aged 36 – 83 years, who were divided into two
groups – control group (CG), group with diagnosed endocrine disease
(DED). The concentration of PTH and TSH, Ca and CHOL was
measured through the medium of analyzers Cobas e411 (Japan);
Cobas Integra 400 (Switzerland). At individuals was measured body
weight as well as stature and thereupon from those data we
enumerated BMI. On the basis of Student T-test in biochemical
parameter of PTH and Ca we found out significantly meaningful
difference (p
Abstract: The research focuses on the effects of polyphenols
extracted from Sambucus nigra fruit, using an experimental arterial
hypertension pattern, as well as their influence on the oxidative
stress. The results reveal the normalization of the reduced glutathion
concentration, as well as a considerable reduction in the
malondialdehide serum concentration by the polyphenolic protection.
The rat blood pressure values were recorded using a CODATM
system, which uses a non-invasive blood pressure measuring method.
All the measured blood pressure components revealed a biostatistically
significant (p
Abstract: The effectiveness of consuming a nutrient fortified oat drink on iron, zinc, vitamin A and vitamin C status was assessed among a cohort of school-aged Filipino children. Ultimate study implementation permitted only a within-subject comparison of change in nutritional status after four months of consuming a nutrient fortified oat drink. Thirty-eight anemic children (5-8 years) consumed an oat drink fortified with iron as NaFeEDTA, zinc, vitamin A and vitamin C for 120 days. Height, weight, serum nutrient levels, anemia status and dietary intake were assessed pre and post intervention. Thirty-four anemic children completed the intervention. After 4 months of intervention, prevalence of anemia decreased by 68% and significant improvements in iron and vitamin A status were observed. Results demonstrate the effectiveness of the fortified oat drink in alleviating anemia in young children and highlight the value of fortification programs
Abstract: Kombucha Tea Ferment (KT), was given to male
albino rats, (1ml/Kg of body weight), via gavages, during 2 weeks
before intraperitoneal administration of 3.5 mg/Kg body weight
CdCl2 and/or whole body γ-irradiation with 4Gy, and during 4 weeks
after each treatment. Hepatic and nephritic pathological changes
included significant increases of serum alanine transaminase (ALT),
aspartate transaminase (AST), and alkaline phosphatase (ALP)
activities, and creatinine and urea contents with significant decrease
in serum total antioxidant capacity (TAC). Increase in oxidative
stress markers in liver and kidney tissues expressed by significant
increase in malondialdehyde (MDA) and nitric oxide (NO) contents
associated to significant depletion in superoxide dismutase (SOD)
and catalase (CAT) activities, and reduced glutathione (GSH) content
were recorded. KT administration results in recovery of all the
pathological changes. It could be concluded that KT might protect
liver and kidney from oxidative damage induced by exposure to
cadmium and/ or γ-irradiation.
Abstract: Carbon tetrachloride (CCl4) is a well-known
hepatotoxin and exposure to this chemical is known to induce
oxidative stress and causes liver injury by the formation of free
radicals. Flacourtia indica commonly known as 'Baichi' has been
reported as an effective remedy for the treatment of a variety of
diseases. The objective of this study was to investigate the
hepatoprotective activity of aqueous extract of leaves of Flacourtia
indica against CCl4 induced hepatotoxicity. Animals were pretreated
with the aqueous extract of Flacourtia indica (250 & 500 mg/kg
body weight) for one week and then challenged with CCl4 (1.5 ml/kg
bw) in olive oil (1:1, v/v) on 7th day. Serum marker enzymes (ALP,
AST, ALT, Total Protein & Total Bilirubin) and TBARS level
(Marker for oxidative stress) were estimated in all the study groups.
Alteration in the levels of biochemical markers of hepatic damage
like AST, ALT, ALP, Total Protein, Total Bilirubin and lipid
peroxides (TBARS) were tested in both CCl4 treated and extract
treated groups. CCl4 has enhanced the AST, ALT, ALP and the
Lipid peroxides (TBARS) in liver. Treatment of aqueous extract of
Flacourtia indica leaves (250 & 500 mg/kg) exhibited a significant
protective effect by altering the serum levels of AST, ALT, ALP,
Total Protein, Total Bilirubin and liver TBARS. These biochemical
observations were supported by histopathological study of liver
sections. From this preliminary study it has been concluded that the
aqueous extract of the leaves of Flacourtia indica protects liver
against oxidative damages and could be used as an effective protector
against CCl4 induced hepatic damage. Our findings suggested that
Flacourtia indica possessed good hepatoprotective activity
Abstract: An application of the highly biosensor based on pH-sensitive field immobilized urease for urea analysis was demo The main analytical characteristics of the bios determined; the conditions of urea measureme blood were optimized. A conceptual possibility biosensor for detection of urea concentratio patients suffering from renal insufficiency was sensitive and selective effect transistor and monstrated in this work. iosensor developed were ment in real samples of ility of application of the tion in blood serum of as shown.