Effect of Crude oil Intoxication on Antioxidant and Marker Enzymes of Tissue Damage in Liver of Rat
The objective of the present study was to examine the
dose-response relationships between antioxidant parameters and liver
contaminant levels of Kazakhstan light crude oil (KLCO) in albino
rats. The animals were repeatedly exposed, by intraperitoneal
injection, to low dosages (0.5–1.5 ml/kg) of KLCO. Rats exposed to
these doses levels did not show any apparent symptoms of
intoxication. Serum aminotransferases increased significantly
(p<0.05) while after 8-d recovered to control level. hepatic
superoxide dismutase (SOD) activity significantly increased after 1
and 3-d except with 0.5 ml while with increased exposure time it
deceased insignificantly after 5-d and significantly after 8-d. Dose
and time-dependent increases were observed in the levels of hepatic
glutathione-S-transeferase (GST) and conjugated diene (CD) while
malondialdehyde (MDA) was evolved after 8 with the dose of 1.5 ml.
the data obtained indicate impaired liver function in the rats
intoxicated with KLCO as manifested by elevated liver enzymes.
[1] O.E. Orisakwe, D.D. Akumka, A.A. Njan and O.J. Afonne, "Testicular
toxicity of Nigerian bonny light crude oil in male albino rats"
Reproductive Toxicology, 18: 439-442, 2004.
[2] R.W. Coppock, M.S. Mostrom, A.A. Khan and S.S. Semalulu,
"Toxicology of oil field pollutants in cattle" a review. Vet. Hum.
Toxicol., 37(6): 569-576, 1995.
[3] S.D. Garrity, S.C. Levings, "Effects of oil spill on the gastropods of a
tropical inter-tidal reef flat" Mar Environ Res., 30:119-153, 1990. Doi:
10.1016/0141-1136(90)90014-F.
[4] B. Freedman, "Oil pollution. In: Environmental Ecology: The Ecological
Effects of Pollution, Disturbances and other Stresses" (Freedman, B.,
Ed.). 2nd ed. Academic Press, San Diego, CA. pp. 159-188, 1995.
[5] G.R. Gardner, P.P. Yevich, J.C. Harshbarger and A.R. Malcolm,
"Carcinogenicity of Black Rock Harbor sediment to the eastern oyster
and tropic transfer of Black Rock Harbor carcinogens from the blue
mussel to the winter flounder" Environ. Health Perspect., 90: 53-66,
1991.
[6] R. Altenburger, M. Nendza, G. Schuurmann, "Mixture toxicity and its
modeling by quantitative structure-activity relationships. Environ
Toxicol Chem., 22:1900-1915, 2003.
[7] D.S. Young, 1990 "Effect of drugs on clinical laboratory tests" 3 rd.
American Association for Clinical Chemistry.
[8] M. Nishikimi, N.A. Rao and K. Yagi, "The occurrence of superoxide
anion in the reaction of reduced phenazine methosulfate and molecular
oxygen" Biochem. Biophys. Res. Commun., 46(2):849-854, 1972.
[9] W.H. Habig, M.J. Pabst and W.B. Jakoby, "Glutathione S-transferases:
the first enzymatic step in mercapturic acid formation" J. Biol.Chem.,
249(22):7130-7139, 1974.
[10] E.B. Burlakova, A.V. ðÉlesenko, E.M. Molochkina, N.P. Palmina and
N.G. Khrapova, "Bioantioxidants for radiation damage and malignant
growth" Nauka, Moscow, 214p, 1975.
[11] S.C. Lin, T.C. Chung, T.H. Ueng, Y.H. Linn, S.H. Hsu, C.L. Chiang and
Lin, "The hepatoprotective effects of Solanum alatam moech on
acetaminophen-Induced hepatotoxicity in mice" Am. J. Clin. Med., 28:
105-114, 2000.
[12] G.J. Beckett, G.R. Foster, A.D. Hussey, D.B.G. Oliveira, J.W. Donovan,
L.F. Presscott and A.T. Proudfoot, "Plasma gluththione s-transferase and
F-protein are more sensitive than alanine aminotransferase as markers of
paracetamol (acetaminophen) - induced liver damage" Clin. Chem., 35:
2186-2189, 1989.
[13] N. McIntyre and S. Rosalki, "Biochemical Investigation in the
Management of Liver Disease. In: Hepatobiliary Diseases" Prieto, J., J.
Rodes and D.A. Shafritz (Eds.). Springer-Verlag, Berlin, pp: 39-71,
1992.
[14] D.R. Livingstone, "Contaminant stimulated reactive oxygen species
production and oxidative damage in aquatic organisms" Marine
Pollution Bulletin, 42(8):656-666, 2001.
[15] A.D. Correia, R. goncalves, M. Scholze, M. Ferreira and M.A.
Henrigues, "Biochemical and behavioral responses in gill head seabream
(Sperus auratus) to phenanthrene" J. Exper. Marine Boil & Ecology.,
347:109-122, 2007.
[16] O.R. Van der, J. Beyer and N.P. Vermeulen, "Fish bioaccumulation and
biomarkers in environmental risk assessment" a review. Environmental
Toxicology and Pharmacology, 13(2):57-149, 2003.
[17] F.I. Achuba, S.A. Osakwe, "Petroleum induced free radical toxicity in
African Catfish (Clarias gariepinus)" Fish. Physiol. Biochem., 29 :(2)
97-103, 2003.
[18] M. Mulero, M. Romeo, M. Giralt, J. Folch, et al., "Oxidative stressrelated
markers and Langerhans cells in a hairless rat model exposed to
UV radiation" J. Toxicol. Environ Health A, 69(14):1371-1385, 2006.
[19] C. Douillet, M. Bost, M. Accominotti, F. Borson-Chazot and M.
Ciavatti, "Effect of Selenium and Vitamin E Supplements on Tissue
Lipids, Peroxides, and Fatty Acid Distribution in Experimental
Diabetes" Lipids, 33: (4), 1998.
[1] O.E. Orisakwe, D.D. Akumka, A.A. Njan and O.J. Afonne, "Testicular
toxicity of Nigerian bonny light crude oil in male albino rats"
Reproductive Toxicology, 18: 439-442, 2004.
[2] R.W. Coppock, M.S. Mostrom, A.A. Khan and S.S. Semalulu,
"Toxicology of oil field pollutants in cattle" a review. Vet. Hum.
Toxicol., 37(6): 569-576, 1995.
[3] S.D. Garrity, S.C. Levings, "Effects of oil spill on the gastropods of a
tropical inter-tidal reef flat" Mar Environ Res., 30:119-153, 1990. Doi:
10.1016/0141-1136(90)90014-F.
[4] B. Freedman, "Oil pollution. In: Environmental Ecology: The Ecological
Effects of Pollution, Disturbances and other Stresses" (Freedman, B.,
Ed.). 2nd ed. Academic Press, San Diego, CA. pp. 159-188, 1995.
[5] G.R. Gardner, P.P. Yevich, J.C. Harshbarger and A.R. Malcolm,
"Carcinogenicity of Black Rock Harbor sediment to the eastern oyster
and tropic transfer of Black Rock Harbor carcinogens from the blue
mussel to the winter flounder" Environ. Health Perspect., 90: 53-66,
1991.
[6] R. Altenburger, M. Nendza, G. Schuurmann, "Mixture toxicity and its
modeling by quantitative structure-activity relationships. Environ
Toxicol Chem., 22:1900-1915, 2003.
[7] D.S. Young, 1990 "Effect of drugs on clinical laboratory tests" 3 rd.
American Association for Clinical Chemistry.
[8] M. Nishikimi, N.A. Rao and K. Yagi, "The occurrence of superoxide
anion in the reaction of reduced phenazine methosulfate and molecular
oxygen" Biochem. Biophys. Res. Commun., 46(2):849-854, 1972.
[9] W.H. Habig, M.J. Pabst and W.B. Jakoby, "Glutathione S-transferases:
the first enzymatic step in mercapturic acid formation" J. Biol.Chem.,
249(22):7130-7139, 1974.
[10] E.B. Burlakova, A.V. ðÉlesenko, E.M. Molochkina, N.P. Palmina and
N.G. Khrapova, "Bioantioxidants for radiation damage and malignant
growth" Nauka, Moscow, 214p, 1975.
[11] S.C. Lin, T.C. Chung, T.H. Ueng, Y.H. Linn, S.H. Hsu, C.L. Chiang and
Lin, "The hepatoprotective effects of Solanum alatam moech on
acetaminophen-Induced hepatotoxicity in mice" Am. J. Clin. Med., 28:
105-114, 2000.
[12] G.J. Beckett, G.R. Foster, A.D. Hussey, D.B.G. Oliveira, J.W. Donovan,
L.F. Presscott and A.T. Proudfoot, "Plasma gluththione s-transferase and
F-protein are more sensitive than alanine aminotransferase as markers of
paracetamol (acetaminophen) - induced liver damage" Clin. Chem., 35:
2186-2189, 1989.
[13] N. McIntyre and S. Rosalki, "Biochemical Investigation in the
Management of Liver Disease. In: Hepatobiliary Diseases" Prieto, J., J.
Rodes and D.A. Shafritz (Eds.). Springer-Verlag, Berlin, pp: 39-71,
1992.
[14] D.R. Livingstone, "Contaminant stimulated reactive oxygen species
production and oxidative damage in aquatic organisms" Marine
Pollution Bulletin, 42(8):656-666, 2001.
[15] A.D. Correia, R. goncalves, M. Scholze, M. Ferreira and M.A.
Henrigues, "Biochemical and behavioral responses in gill head seabream
(Sperus auratus) to phenanthrene" J. Exper. Marine Boil & Ecology.,
347:109-122, 2007.
[16] O.R. Van der, J. Beyer and N.P. Vermeulen, "Fish bioaccumulation and
biomarkers in environmental risk assessment" a review. Environmental
Toxicology and Pharmacology, 13(2):57-149, 2003.
[17] F.I. Achuba, S.A. Osakwe, "Petroleum induced free radical toxicity in
African Catfish (Clarias gariepinus)" Fish. Physiol. Biochem., 29 :(2)
97-103, 2003.
[18] M. Mulero, M. Romeo, M. Giralt, J. Folch, et al., "Oxidative stressrelated
markers and Langerhans cells in a hairless rat model exposed to
UV radiation" J. Toxicol. Environ Health A, 69(14):1371-1385, 2006.
[19] C. Douillet, M. Bost, M. Accominotti, F. Borson-Chazot and M.
Ciavatti, "Effect of Selenium and Vitamin E Supplements on Tissue
Lipids, Peroxides, and Fatty Acid Distribution in Experimental
Diabetes" Lipids, 33: (4), 1998.
@article{"International Journal of Biological, Life and Agricultural Sciences:55359", author = "K. Mahmoud and T. Shalahmetova and Sh. Deraz", title = "Effect of Crude oil Intoxication on Antioxidant and Marker Enzymes of Tissue Damage in Liver of Rat", abstract = "The objective of the present study was to examine the
dose-response relationships between antioxidant parameters and liver
contaminant levels of Kazakhstan light crude oil (KLCO) in albino
rats. The animals were repeatedly exposed, by intraperitoneal
injection, to low dosages (0.5–1.5 ml/kg) of KLCO. Rats exposed to
these doses levels did not show any apparent symptoms of
intoxication. Serum aminotransferases increased significantly
(p", keywords = "Crude oil; superoxide dismutase, glutathione-Stranseferase,malondialdehyde, conjugated diene, aminotransferases.", volume = "5", number = "11", pages = "721-3", }
