The Effects of Food Deprivation on Hematological Indices and Blood Indicators of Liver Function in Oxyleotris marmorata
Oxyleotris marmorata is considered as
undomesticated fish, and its culture occasionally faces a problem of
food deprivation. The present study aims to evaluate alteration of
hematological indices, blood chemical associated with liver function
during 4 weeks of fasting. A non-linear relationships between fasting
days and hematological parameters (red blood cell number; y = -
0.002x2 + 0.041x + 1.249; R2=0.915, P<0.05, hemoglobin; y = -
0.002x2 + 0.030x + 3.470; R2=0.460, P>0.05), mean corpuscular
volume; y = -0.180x2 + 2.183x + 149.61; R2=0.732, P>0.05, mean
corpuscular hemoglobin; y = -0.041x2 + 0.862x + 29.864; R2=0.818,
P>0.05 and mean corpuscular hemoglobin concentration; y = -
0.044x2 + 0.711x + 21.580; R2=0.730, P>0.05) were demonstrated.
Significant change in hematocrit (Ht) during fasting period was
observed. Ht elevated sharply increase at the first weeks of fasting
period. Higher Ht also was detected during week 2-4 of fasting time.
The significant reduction of hepatosomatic index was observed (y = -
0.007x2 - 0.096x + 1.414; R2=0.968, P<0.05). Moreover, alteration
of enzyme associated with liver function was evaluated during 4
weeks of fasting (alkalin phosphatase; y = -0.026x2 - 0.935x +
12.188; R2=0.737, P>0.05, serum glutamic oxaloacetic transaminase;
y = 0.005x2 – 0.201x2 + 1.297x + 33.256; R2=1, P<0.01, serum
glutamic pyruvic transaminase; y = 0.007x2 – 0.274x2 + 2.277x +
25.257; R2=0.807, P>0.05). Taken together, prolonged fasting has
deleterious effects on hematological indices, liver mass and enzyme
associated in liver function. The marked adverse effects occurred
after the first week of fasting state.
[1] T. Wang, C.C.Y. Hung, D.J. Randall, "The comparative physiology of
food deprivation: From feast to famine," Annu. Rev. Physiol., vol. 68,
pp. 223-251, Oct. 2006.
[2] R. Silveira-Coffigny, A. Prieto-Trujillo, F., Ascencio-Valle, F., "Effects
of different stressors in haematological variables in cultured
Oreochromis aureus," Comp. Biochem. Physiol. vol 139, pp. 245-250.
2004.
[3] T. Pitaksong, P. Kuppapitayanan, S. Boonanuntanasarn, "The effects of
vitamins C and E on the growth, tissue accumulation, and prophylactic
response to thermal and acidic stress of hybrid catfish," Aqual. Nutri.
Accepted.
[4] A.J. Gingerich, D.P. Philipp, C.D. Suski, "Effects of nutritional status on
metabolic rate, exercise and recovery in a freshwater fish," J. Comp.
Physiol. B vol. 180, pp. 371-384, 2010.
[5] F.S. Rios, C.S. Carvalho, G.H.D. Pinheiro, L. Donatti, M.N. Fernandes,
F.T. Rantin, "Utilization of endogenous reserves and effects of
starvation on the health of Prochilodus lineatus (Prochilodontidae),"
Environ. Biol. Fish, vol. 91, pp. 87-94, 2011.
[6] C.A. Shoemaker, P.H. Klesius, C. Lim, M. Yildirim, "Feed deprivation
of channel catfish, Ictalurus punctatus (Rafinesque), influences
organosomatic indices, chemical composition and susceptibility to
Flavobacterium columnare," J. Fish Dis. Vol. 25, pp. 558-561, 2003.
[7] R. Laiz-Carrion, I.R. Viana, J.R. Cejas, I.R-Jarabo, S. Jerez, J.A. Martos,
A.B. Eduardo, J.M. Mancera, "Influence of food deprivation and high
stocking density in energetic metabolism and stress response in red
progy, Pagrus pagrus L," Aquacult Int., vol. 15 p., Dec. 2011.
[8] S. Palakof, F.J. Arjona, S.Sangiao-Alvarellos, M.P. Martin del Rio, J.M.
Mancera, J.L. Soengas, "Food deprivation alters osmoregulatory and
metabolic responses to salinity acclimation in gilthead sea bream Sparus
auratus," J. comp. Physiol. B, vol. 176, pp. 441-452, 2006.
[9] A.E. Morales, A.Perez-Jimenez, M.C. Hidalgo, E. Abellan, G.
Cardenete, "Oxidative stress and antioxidant defense after prolonged
starvation in Dentex dentex liver," Comp. Biochem. Physiol. Part C. vol.
139, pp. 153-161, 2004.
[10] J.L. congleton, T. Wagner, "Blood-chemistry indicators of nutritional
status in juvenile salmonids," J. Fish Biol., vol. 69, pp. 473-490, 2006.
[11] G.L. Voigt, "Hematology techniques and concepts for veterinary
technicians," Iowa State University Press. pp.139, 2000.
[12] S. Reitman, S. Frankel, "A colorimetric method for the determination of
serum glutamic oxaloacteic and glutamic phyruvic transaminase," Am.
J. Clin, Pathol., vol. 28, pp. 56-63. 1957.
[13] A.L. Babson, S.J. Greeley, C.M.coleman, G.E. Phillips,
"Phenolphthalein monophosphate as a substrate for serum alkaline
phosphatase," Clin. Chem., vol. 12, pp. 482, 1966.
[14] G.D. Delgiudice, "Effects of feeding and fasting on wolf blood and urine
characteristics," J. Wildl. Manage., vol. 51, pp. 1-10, 1987.
[15] M.T. Allen, S.M. Patterson, "Hemoconcentration and stress: a review of
physiological mechanisms and relevance for cardiovascular disease
risk," Biol. Psychol. Vol. 41 pp.1-27, 1995.
[16] M. Nikinmaa, W.H. Huestis, "Adrenergic swelling of nucleated
erythrocytes: cellular mechanisms in a bird, domestic goose, and two
teleosts, striped bass and rainbow trout," J. Exp. Biol. Vol. 113, pp.215-
224, 1984.
[17] N. De Pedro, M.J. Delgado, B. Gancedo, M. Alonso-Bedate, "Changes
in glucose, glycogen, thyroid activity and hypothalamic catecholamines
in tench by starvation and refeeding," J. comp. Physiol. B., vol. 173, pp.
475-481, 2003.
[18] E.M. Hevroy, C. Azpeleta, M. Shimizu, A. Lanzen, H. Kaiya, M. Espe,
P.A. Olsvik, Effects of short-term starvation on ghrelin, GH-IGF system,
and IGF-binding proteins in Atlantic salmon, Fish Physiol. biochem.,
vol. 37, pp. 217-232. 2011.
[19] E. Gisvert, I. Fernadez, C.A. Alvarez-Gongzalez, "Prolonged feed
deprivation does not permanently compromise digestive function in
migration European glass eels Anguilla Anguilla," J. Fish Biol. Vol. 78,
pp. 580-592. 2011.
[20] B.N. Chen, J.G. Qin, J.F. Carragher, S.M. Clarke, M.S. Kumar, W.G.
Hutchinson, "Deleterious effects of food restrictions in yelklowtail
kingfish Seriola lalandi during early development," Aquaculure, vol.
271, pp. 326-335, 2007.
[1] T. Wang, C.C.Y. Hung, D.J. Randall, "The comparative physiology of
food deprivation: From feast to famine," Annu. Rev. Physiol., vol. 68,
pp. 223-251, Oct. 2006.
[2] R. Silveira-Coffigny, A. Prieto-Trujillo, F., Ascencio-Valle, F., "Effects
of different stressors in haematological variables in cultured
Oreochromis aureus," Comp. Biochem. Physiol. vol 139, pp. 245-250.
2004.
[3] T. Pitaksong, P. Kuppapitayanan, S. Boonanuntanasarn, "The effects of
vitamins C and E on the growth, tissue accumulation, and prophylactic
response to thermal and acidic stress of hybrid catfish," Aqual. Nutri.
Accepted.
[4] A.J. Gingerich, D.P. Philipp, C.D. Suski, "Effects of nutritional status on
metabolic rate, exercise and recovery in a freshwater fish," J. Comp.
Physiol. B vol. 180, pp. 371-384, 2010.
[5] F.S. Rios, C.S. Carvalho, G.H.D. Pinheiro, L. Donatti, M.N. Fernandes,
F.T. Rantin, "Utilization of endogenous reserves and effects of
starvation on the health of Prochilodus lineatus (Prochilodontidae),"
Environ. Biol. Fish, vol. 91, pp. 87-94, 2011.
[6] C.A. Shoemaker, P.H. Klesius, C. Lim, M. Yildirim, "Feed deprivation
of channel catfish, Ictalurus punctatus (Rafinesque), influences
organosomatic indices, chemical composition and susceptibility to
Flavobacterium columnare," J. Fish Dis. Vol. 25, pp. 558-561, 2003.
[7] R. Laiz-Carrion, I.R. Viana, J.R. Cejas, I.R-Jarabo, S. Jerez, J.A. Martos,
A.B. Eduardo, J.M. Mancera, "Influence of food deprivation and high
stocking density in energetic metabolism and stress response in red
progy, Pagrus pagrus L," Aquacult Int., vol. 15 p., Dec. 2011.
[8] S. Palakof, F.J. Arjona, S.Sangiao-Alvarellos, M.P. Martin del Rio, J.M.
Mancera, J.L. Soengas, "Food deprivation alters osmoregulatory and
metabolic responses to salinity acclimation in gilthead sea bream Sparus
auratus," J. comp. Physiol. B, vol. 176, pp. 441-452, 2006.
[9] A.E. Morales, A.Perez-Jimenez, M.C. Hidalgo, E. Abellan, G.
Cardenete, "Oxidative stress and antioxidant defense after prolonged
starvation in Dentex dentex liver," Comp. Biochem. Physiol. Part C. vol.
139, pp. 153-161, 2004.
[10] J.L. congleton, T. Wagner, "Blood-chemistry indicators of nutritional
status in juvenile salmonids," J. Fish Biol., vol. 69, pp. 473-490, 2006.
[11] G.L. Voigt, "Hematology techniques and concepts for veterinary
technicians," Iowa State University Press. pp.139, 2000.
[12] S. Reitman, S. Frankel, "A colorimetric method for the determination of
serum glutamic oxaloacteic and glutamic phyruvic transaminase," Am.
J. Clin, Pathol., vol. 28, pp. 56-63. 1957.
[13] A.L. Babson, S.J. Greeley, C.M.coleman, G.E. Phillips,
"Phenolphthalein monophosphate as a substrate for serum alkaline
phosphatase," Clin. Chem., vol. 12, pp. 482, 1966.
[14] G.D. Delgiudice, "Effects of feeding and fasting on wolf blood and urine
characteristics," J. Wildl. Manage., vol. 51, pp. 1-10, 1987.
[15] M.T. Allen, S.M. Patterson, "Hemoconcentration and stress: a review of
physiological mechanisms and relevance for cardiovascular disease
risk," Biol. Psychol. Vol. 41 pp.1-27, 1995.
[16] M. Nikinmaa, W.H. Huestis, "Adrenergic swelling of nucleated
erythrocytes: cellular mechanisms in a bird, domestic goose, and two
teleosts, striped bass and rainbow trout," J. Exp. Biol. Vol. 113, pp.215-
224, 1984.
[17] N. De Pedro, M.J. Delgado, B. Gancedo, M. Alonso-Bedate, "Changes
in glucose, glycogen, thyroid activity and hypothalamic catecholamines
in tench by starvation and refeeding," J. comp. Physiol. B., vol. 173, pp.
475-481, 2003.
[18] E.M. Hevroy, C. Azpeleta, M. Shimizu, A. Lanzen, H. Kaiya, M. Espe,
P.A. Olsvik, Effects of short-term starvation on ghrelin, GH-IGF system,
and IGF-binding proteins in Atlantic salmon, Fish Physiol. biochem.,
vol. 37, pp. 217-232. 2011.
[19] E. Gisvert, I. Fernadez, C.A. Alvarez-Gongzalez, "Prolonged feed
deprivation does not permanently compromise digestive function in
migration European glass eels Anguilla Anguilla," J. Fish Biol. Vol. 78,
pp. 580-592. 2011.
[20] B.N. Chen, J.G. Qin, J.F. Carragher, S.M. Clarke, M.S. Kumar, W.G.
Hutchinson, "Deleterious effects of food restrictions in yelklowtail
kingfish Seriola lalandi during early development," Aquaculure, vol.
271, pp. 326-335, 2007.
@article{"International Journal of Biological, Life and Agricultural Sciences:54863", author = "N. Sridee and S. Boonanuntanasarn", title = "The Effects of Food Deprivation on Hematological Indices and Blood Indicators of Liver Function in Oxyleotris marmorata", abstract = "Oxyleotris marmorata is considered as
undomesticated fish, and its culture occasionally faces a problem of
food deprivation. The present study aims to evaluate alteration of
hematological indices, blood chemical associated with liver function
during 4 weeks of fasting. A non-linear relationships between fasting
days and hematological parameters (red blood cell number; y = -
0.002x2 + 0.041x + 1.249; R2=0.915, P0.05), mean corpuscular
volume; y = -0.180x2 + 2.183x + 149.61; R2=0.732, P>0.05, mean
corpuscular hemoglobin; y = -0.041x2 + 0.862x + 29.864; R2=0.818,
P>0.05 and mean corpuscular hemoglobin concentration; y = -
0.044x2 + 0.711x + 21.580; R2=0.730, P>0.05) were demonstrated.
Significant change in hematocrit (Ht) during fasting period was
observed. Ht elevated sharply increase at the first weeks of fasting
period. Higher Ht also was detected during week 2-4 of fasting time.
The significant reduction of hepatosomatic index was observed (y = -
0.007x2 - 0.096x + 1.414; R2=0.968, P0.05, serum glutamic oxaloacetic transaminase;
y = 0.005x2 – 0.201x2 + 1.297x + 33.256; R2=1, P0.05). Taken together, prolonged fasting has
deleterious effects on hematological indices, liver mass and enzyme
associated in liver function. The marked adverse effects occurred
after the first week of fasting state.", keywords = "food deprivation, Oxyleotris marmorata,
hematology, alkaline phosphatase, SGOT, SGPT", volume = "6", number = "5", pages = "282-5", }
