Abstract: Epidermal Growth Factor (EGF, Mw=6,045) has been
reported to have high efficiency of wound repair and anti-wrinkle
effect. However, the half-life of EGF in the body is too short to exert
the biological activity effectively when applied in free form. Growth
Factors can be stabilized by immobilization with carbohydrates from
thermal and proteolytic degradation. Low molecular weight chitosan
(LMCS) and its derivate prepared by hydrogen peroxide has high
solubility. LM6A6DC was successfully prepared as a reactive
carbohydrate for the stabilization of EGF by the reactions of LMCS
with alkalization, tosylation, azidation and reduction. The structure of
LM6A6DC was confirmed by FT-IR, 1H NMR and elementary
analysis. For enhancing the stability of free EGF, EGF was attached
with LM6A6DC by using water-soluble carbodiimide.
EGF-LM6A6DC conjugates did not show any cytotoxicity on the
Normal Human Dermal Fibroblast (NHDF) 3T3 proliferation at least
under 100 μg/ml. In the result, it was considered that LM6A6DC is
suitable to immobilize of growth factor.
Abstract: Over the past few years, the online multimedia
collection has grown at a fast pace. Several companies showed
interest to study the different ways to organise the amount of audio
information without the need of human intervention to generate
metadata. In the past few years, many applications have emerged on
the market which are capable of identifying a piece of music in a
short time. Different audio effects and degradation make it much
harder to identify the unknown piece. In this paper, an audio
fingerprinting system which makes use of a non-parametric based
algorithm is presented. Parametric analysis is also performed using
Gaussian Mixture Models (GMMs). The feature extraction methods
employed are the Mel Spectrum Coefficients and the MPEG-7 basic
descriptors. Bin numbers replaced the extracted feature coefficients
during the non-parametric modelling. The results show that nonparametric
analysis offer potential results as the ones mentioned in
the literature.
Abstract: Natural hydrocarbon seepage has helped petroleum
exploration as a direct indicator of gas and/or oil subsurface
accumulations. Surface macro-seeps are generally an indication of a
fault in an active Petroleum Seepage System belonging to a Total
Petroleum System. This paper describes a case study in which
multiple analytical techniques were used to identify and characterize
trace petroleum-related hydrocarbons and other volatile organic
compounds in groundwater samples collected from Sousse aquifer
(Central Tunisia). The analytical techniques used for analyses of
water samples included gas chromatography-mass spectrometry (GCMS),
capillary GC with flame-ionization detection, Compound
Specific Isotope Analysis, Rock Eval Pyrolysis. The objective of the
study was to confirm the presence of gasoline and other petroleum
products or other volatile organic pollutants in those samples in order
to assess the respective implication of each of the potentially
responsible parties to the contamination of the aquifer. In addition,
the degree of contamination at different depths in the aquifer was also
of interest. The oil and gas seeps have been investigated using
biomarker and stable carbon isotope analyses to perform oil-oil and
oil-source rock correlations. The seepage gases are characterized by
high CH4 content, very low δ13CCH4 values (-71,9 ‰) and high
C1/C1–5 ratios (0.95–1.0), light deuterium–hydrogen isotope ratios (-
198 ‰) and light δ13CC2 and δ13CCO2 values (-23,8‰ and-23,8‰
respectively) indicating a thermogenic origin with the contribution of
the biogenic gas. An organic geochemistry study was carried out on
the more ten oil seep samples. This study includes light hydrocarbon
and biomarkers analyses (hopanes, steranes, n-alkanes, acyclic
isoprenoids, and aromatic steroids) using GC and GC-MS. The
studied samples show at least two distinct families, suggesting two
different types of crude oil origins: the first oil seeps appears to be
highly mature, showing evidence of chemical and/or biological
degradation and was derived from a clay-rich source rock deposited
in suboxic conditions. It has been sourced mainly by the lower
Fahdene (Albian) source rocks. The second oil seeps was derived
from a carbonate-rich source rock deposited in anoxic conditions,
well correlated with the Bahloul (Cenomanian-Turonian) source rock.
Abstract: Concrete is found to undergo degradation when
subjected to elevated temperatures and loose substantial amount of its
strength. The loss of strength in concrete is mainly attributed to
decomposition of C-S-H and release of physically and chemically
bound water, which begins when the exposure temperature exceeds
100°C. When such a concrete comes in contact with moisture, the
cement paste is found rehydrate and considerable amount of strength
lost is found to recover. This paper presents results of an
experimental program carried out to investigate the effect of recuring
on strength gain of OPC concrete specimens subjected to elevated
temperatures from 200°C to 800°C, which were subjected to
retention time of two hours and four hours at the designated
temperature. Strength recoveries for concrete subjected to 7
designated elevated temperatures are compared. It is found that the
efficacy of recuring as a measure of strength recovery reduces with
increase in exposure temperature.
Abstract: For centuries humans have used the antimicrobial
properties of copper to their advantage. Yet, after all these years the
underlying mechanisms of copper mediated cell death in various
microbes remain unclear. We had explored the hypothesis that copper
mediated increased levels of lipid peroxidation in the membrane fatty
acids is responsible for increased killing in Escherichia coli. In this
study we show that in both gram positive (Staphylococcus aureus)
and gram negative (Pseudomonas aeruginosa) bacteria there is a
strong correlation between copper mediated cell death and increased
levels of lipid peroxidation. Interestingly, the non-spore forming
gram positive bacteria as well as gram negative bacteria show similar
patterns of cell death, increased levels of lipid peroxidation, as well
as genomic DNA degradation, however there is some difference in
loss in membrane integrity upon exposure to copper alloy surface.
Abstract: Biofuels production has come forth as a future
technology to combat the problem of depleting fossil fuels. Bio-based
ethanol production from enzymatic lignocellulosic biomass
degradation serves an efficient method and catching the eye of
scientific community. High cost of the enzyme is the major obstacle
in preventing the commercialization of this process. Thus main
objective of the present study was to optimize composition of
medium components for enhancing cellulase production by newly
isolated strain of Bacillus tequilensis. Nineteen factors were taken
into account using statistical Plackett-Burman Design. The significant
variables influencing the cellulose production were further employed
in statistical Response Surface Methodology using Central
Composite Design for maximizing cellulase production. The
optimum medium composition for cellulase production was: peptone
(4.94 g/L), ammonium chloride (4.99 g/L), yeast extract (2.00 g/L),
Tween-20 (0.53 g/L), calcium chloride (0.20 g/L) and cobalt chloride
(0.60 g/L) with pH 7, agitation speed 150 rpm and 72 h incubation at
37oC. Analysis of variance (ANOVA) revealed high coefficient of
determination (R2) of 0.99. Maximum cellulase productivity of 11.5
IU/ml was observed against the model predicted value of 13 IU/ml.
This was found to be optimally active at 60oC and pH 5.5.
Abstract: Thin ZnO films are deposited on glass substrates via
sol–gel method and dip-coating. The films are prepared from zinc
acetate dehydrate as a starting reagent. After that the as-prepared
ZnO sol is aged for different periods (0, 1, 3, 5, 10, 15 and 30 days).
Nanocrystalline thin films are deposited from various sols. The
effect ZnO sols aging time on the structural and photocatalytic
properties of the films is studied. The films surface is studied by
Scanning Electron Microscopy. The effect of the aging time of the
starting solution is studied in the photocatalytic degradation of
Reactive Black 5 (RB5) by UV-vis spectroscopy. The experiments
are conducted upon UV-light illumination and in complete darkness.
The variation of the absorption spectra shows the degradation of RB5
dissolved in water, as a result of the reaction, occurring on the surface
of the films and promoted by UV irradiation. The initial
concentrations of dye (5, 10 and 20 ppm) and the effect of the aging
time are varied during the experiments. The results show, that the
increasing aging time of starting solution with respect to ZnO
generally promotes photocatalytic activity. The thin films obtained
from ZnO sol, which is aged 30 days have best photocatalytic
degradation of the dye (97,22%) in comparison with the freshly
prepared ones (65,92%). The samples and photocatalytic
experimental results are reproducible. Nevertheless, all films exhibit
a substantial activity in both UV light and darkness, which is
promising for the development of new ZnO photocatalysts by sol-gel
method.
Abstract: Bacterial strains capable of degradation of malathion
from the domestic sewage were isolated by an enrichment culture
technique. Three bacterial strains were screened and identified as
Acinetobacter baumannii (AFA), Pseudomonas aeruginosa (PS1),
and Pseudomonas mendocina (PS2) based on morphological,
biochemical identification and 16S rRNA sequence analysis.
Acinetobacter baumannii AFA was the most efficient malathion
degrading bacterium, so used for further biodegradation study. AFA
was able to grow in mineral salt medium (MSM) supplemented with
malathion (100 mg/l) as a sole carbon source, and within 14 days,
84% of the initial dose was degraded by the isolate measured by high
performance liquid chromatography. Strain AFA could also degrade
other organophosphorus compounds including diazinon, chlorpyrifos
and fenitrothion. The effect of different culture conditions on the
degradation of malathion like inoculum density, other carbon or
nitrogen sources, temperature and shaking were examined.
Degradation of malathion and bacterial cell growth were accelerated
when culture media were supplemented with yeast extract, glucose
and citrate. The optimum conditions for malathion degradation by
strain AFA were; an inoculum density of 1.5x 10^12CFU/ml at 30°C
with shaking. A specific polymerase chain reaction primers were
designed manually using multiple sequence alignment of the
corresponding carboxylesterase enzymes of Acinetobacter species.
Sequencing result of amplified PCR product and phylogenetic
analysis showed low degree of homology with the other
carboxylesterase enzymes of Acinetobacter strains, so we suggested
that this enzyme is a novel esterase enzyme. Isolated bacterial strains
may have potential role for use in bioremediation of malathion
contaminated.
Abstract: Pipe failure and leakage is a problematic issue and the
traditional solution of replacing the pipes is costly and time
consuming. Rehabilitation by relining materials based on polymer
composites is an alternative solution towards the degradation problem
of the old piping. This paper provides a brief summary of advances in
technology, methods and materials for relining as well as a summary
of the degradation analyses of the two main composite materials used
for relining, rubber filled epoxy and reinforced polyester baltoflake
when they are exposed in deionized water and elevated temperature
up to 80°C for a duration of 2-14 months in the laboratory.
Abstract: Food is widely packaged with plastic materials to
prevent microbial contamination and spoilage. Ionizing radiation is
widely used to sterilize the food-packaging materials. Sterilization by
γ-radiation causes degradation such as embrittlement, stiffening,
softening, discoloration, odour generation, and decrease in molecular
weight. Many antioxidants can prevent γ-degradation but most of
them are toxic. The migration of antioxidants to its environment
gives rise to major concerns in case of food packaging plastics. In
this attempt, we have aimed to utilize synergistic mixtures of
stabilizers which are approved for food-contact applications.
Ethylene-propylene-diene terpolymer has been melt-mixed with
hindered amine stabilizers (HAS), phenolic antioxidants and organophosphites
(hydroperoxide decomposer). Results were discussed by
comparing the stabilizing efficiency of mixtures with and without
phenol system. Among phenol containing systems where we mostly
observed discoloration due to the oxidation of hindered phenol, the
combination of secondary HAS, tertiary HAS, organo-phosphite and
hindered phenol exhibited improved stabilization efficiency than
single or binary additive systems. The mixture of secondary HAS and
tertiary HAS, has shown antagonistic effect of stabilization.
However, the combination of organo-phosphite with secondary HAS,
tertiary HAS and phenol antioxidants have been found to give
synergistic even at higher doses of Gamma-irradiation. The effects
have been explained through the interaction between the stabilizers.
After γ-irradiation, the consumption of oligomeric stabilizer
significantly depends on the components of stabilization mixture. The
effect of the organo-phosphite antioxidant on the overall stability has
been discussed.
Abstract: Recently, increasing the quality of experience (QoE) is
an important issue. Since performance degradation at cell edge
extremely reduces the QoE, several techniques are defined at
LTE/LTE-A standard to remove inter-cell interference (ICI). However,
the conventional techniques have disadvantage because there is a
trade-off between resource allocation and reliable communication.
The proposed scheme reduces the ICI more efficiently by using
channel state information (CSI) smartly. It is shown that the proposed
scheme can reduce the ICI with fewer resources.
Abstract: Worldwide, most PILC MV underground cables in use
are approaching the end of their design life; hence, failures are likely
to increase. This paper studies the electric field and potential
distributions within the PILC insulted cable containing common
void-defect. The finite element model of the performance of the
belted PILC MV underground cable is presented. The variation of the
electric field stress within the cable using the Finite Element Method
(FEM) is concentrated. The effects of the void-defect within the
insulation are given. Outcomes will lead to deeper understanding of
the modeling of Paper Insulated Lead Covered (PILC) and electric
field response of belted PILC insulted cable containing void defect.
Abstract: In this paper, we present a robust algorithm to recognize extracted text from grocery product images captured by mobile phone cameras. Recognition of such text is challenging since text in grocery product images varies in its size, orientation,
style, illumination, and can suffer from perspective distortion.
Pre-processing is performed to make the characters scale and
rotation invariant. Since text degradations can not be appropriately
defined using well-known geometric transformations such
as translation, rotation, affine transformation and shearing, we
use the whole character black pixels as our feature vector.
Classification is performed with minimum distance classifier
using the maximum likelihood criterion, which delivers very
promising Character Recognition Rate (CRR) of 89%. We
achieve considerably higher Word Recognition Rate (WRR) of
99% when using lower level linguistic knowledge about product
words during the recognition process.
Abstract: Rhodamine B (RB) is a toxic dye used extensively in
textile industry, which must be remediated before its drainage to
environment. In the present study, supported gold nanoparticles on
commercially available titania and zincite were successfully prepared
and then their activity on the photodegradation of RB under UV A
light irradiation were evaluated. The synthesized photocatalysts were
characterized by ICP, BET, XRD, and TEM. Kinetic results showed
that Au/TiO2 was an inferior photocatalyst to Au/ZnO. This
observation could be attributed to the strong reflection of UV
irradiation by gold nanoparticles over TiO2 support.
Abstract: The investigation on wind turbine degradation was
carried out using the nacelle wind data. The three Vestas V80-2MW
wind turbines of Sungsan wind farm in Jeju Island, South Korea were
selected for this work. The SCADA data of the wind farm for five
years were analyzed to draw power curve of the turbines. It is assumed
that the wind distribution is the Rayleigh distribution to calculate the
normalized capacity factor based on the drawn power curve of the
three wind turbines for each year. The result showed that the reduction
of power output from the three wind turbines occurred every year and
the normalized capacity factor decreased to 0.12%/year on average.
Abstract: Nanocrystalline powders of the lead-free piezoelectric
material, tantalum-substituted potassium sodium niobate
(K0.5Na0.5)(Nb0.9Ta0.1)O3 (KNNT), were produced using a Retsch
PM100 planetary ball mill by setting the milling time to 15h, 20h,
25h, 30h, 35h and 40h, at a fixed speed of 250rpm. The average
particle size of the milled powders was found to decrease from 12nm
to 3nm as the milling time increases from 15h to 25h, which is in
agreement with the existing theoretical model. An anomalous
increase to 98nm and then a drop to 3nm in the particle size were
observed as the milling time further increases to 30h and 40h
respectively. Various sizes of these starting KNNT powders were
used to investigate the effect of milling time on the microstructure,
dielectric properties, phase transitions and piezoelectric properties of
the resulting KNNT ceramics. The particle size of starting KNNT
was somewhat proportional to the grain size. As the milling time
increases from 15h to 25h, the resulting ceramics exhibit
enhancement in the values of relative density from 94.8% to 95.8%,
room temperature dielectric constant (εRT) from 878 to 1213, and
piezoelectric charge coefficient (d33) from 108pC/N to 128pC/N. For
this range of ceramic samples, grain size refinement suppresses the
maximum dielectric constant (εmax), shifts the Curie temperature (Tc)
to a lower temperature and the orthorhombic-tetragonal phase
transition (Tot) to a higher temperature. Further increase of milling
time from 25h to 40h produces a gradual degradation in the values of
relative density, εRT, and d33 of the resulting ceramics.
Abstract: The present study was conducted to evaluate the
potential applicability of biological trickling filter system for the
treatment of simulated textile wastewater containing reactive azo
dyes with bacterial consortium under non-sterile conditions. The
percentage decolorization for the treatment of wastewater containing
structurally different dyes was found to be higher than 95% in all
trials. The stable bacterial count of the biofilm on stone media of the
trickling filter during the treatment confirmed the presence,
proliferation, dominance and involvement of the added microbial
consortium in the treatment of textile wastewater. Results of
physicochemical parameters revealed the reduction in chemical
oxygen demand (58.5-75.1%), sulphates (18.9-36.5%), and
phosphates (63.6-73.0%). UV-Visible and FTIR spectroscopy
confirmed decolorization of dye containing wastewater was ultimate
consequence of biodegradation. Toxicological studies revealed the
nontoxic nature of degradative metabolites.
Abstract: Oases are complex and fragile agro-ecosystems. They
have always existed in environments characterized by an arid climate,
scarcity of rainfall, high temperatures and high evaporation. These
palms have grown up despite the severity of the physical
characteristics thanks to the water's existence and irrigation practice.
The oases are generally spread along non-perennial rivers (wadis),
shallow water table or deep artesian groundwater. However, the
sustainability of oasis system is threatened by water scarcity and
declining of water table levels particularly in arid areas. Located in
the southern east area of Morocco, Tafilalet plain encompasses one of
the largest palm groves in the kingdom. In recent years, this area has
become increasingly threatened by water shortage and has seen a
sharp deterioration under the effect of several combined
anthropogenic and climatic factors. The Bayoud disease, successive
years of drought, Hassan Addakhil dam construction etc are all
factors that have affected both water and phoenicicole heritage of the
area. The objective of this study is to understand the interaction
between qualitative and quantitative degradation of groundwater
resources, and the palm grove dynamics, while reviewing the
assumption that groundwater resources contribute in a direct way to
the conservation of this oasis agroecosystem. A historical analysis
tracing both the oasis dynamics and the groundwater evolution has
been established. Data were collected from satellite images, surveys
with different actors (farmers, Regional Office for Agricultural
Development, Basin agency...). They were complemented by a
synthesis of numerous technical reports in the area. The results
showed that within 40 years, the thickness of the groundwater table
has dropped in 50 %. Along with this, there has been a downsizing of
date palm by 50 %. Areas with higher groundwater level were the
least affected by the downsizing. So we can say that the shallow
groundwater contribute significantly and directly to the water supply
of date palm through its root system, and largely ensures the oasis
ecosystem sustainability.
Abstract: This paper illustrates the effect of nano Magnesium
Hydroxide (MH) loading on the thermal properties of Low Density
Polyethylene (LDPE)/Poly (ethylene-co vinyl acetate) (EVA) nano
composite. Thermal studies were conducted, as it understanding is
vital for preliminary development of new polymeric systems.
Thermal analysis of nanocomposite was conducted using thermo
gravimetric analysis (TGA), and differential scanning calorimetry
(DSC). Major finding of TGA indicated two main stages of
degradation process found at (350 ± 25oC) and (480 ± 25oC)
respectively. Nano metal filler expressed better fire resistance as it
stand over high degree of temperature. Furthermore, DSC analysis
provided a stable glass temperature around 51 (±1oC) and captured
double melting point at 84 (±2oC) and 108 (±2oC). This binary
melting point reflects the modification of nano filler to the polymer
matrix forming melting crystals of folded and extended chain. The
percent crystallinity of the samples grew vividly with increasing filler
content. Overall, increasing the filler loading improved the
degradation temperature and weight loss evidently and a better
process and phase stability was captured in DSC.
Abstract: This paper present the experimental work of double
unit tunnel form building (TFB) subjected to in-plane lateral cyclic
loading. A one third scale of 3-storey double unit of TFB is tested
until its strength degradation. Then, the TFB is repaired and
retrofitted using additional shear wall, steel angle and CFRP sheet.
The crack patterns, lateral strength, stiffness, ductility and equivalent
viscous damping (EVD) were analyzed and compared before and
after repair and retrofit. The result indicates that the lateral strength
increases by 22% in pushing and 27% in pulling direction. Moreover,
the stiffness and ductility obtained before and after retrofit increase
tremendously by 87.87% and 39.66%, respectively. Meanwhile, the
energy absorption measured by equivalent viscous damping obtained
after retrofit increase by 12.34% in pulling direction. It can be
concluded that the proposed retrofit method is capable to increase the
lateral strength capacity, stiffness and energy absorption of double
unit TFB.