Abstract: Vermiculite was used to develop inorganic
carrier-based formulations of fluorescent pseudomonad strains
R62 and R81. The effect of bio-inoculation of fluorescent
pseudomonad strains R62 and R81 (plant growth promoting
and biocontrol agent) on growth responses of Vigna-mungo
under field condition was enumerated. The combined bioinoculation
of these two organisms in a formuation increased
the pods yield by 300% in comparison to the control crop.
There was also significant increment in the other plant growth
responses such as dry root weight, dry shoot weight, shoot
length and number of branches per plant.
Abstract: Rice seed expression (cDNA) library in the Lambda
Zap 11® phage constructed from the developing grain 10-20 days
after flowering was transformed into yeast for functional
complementation assays in three salt sensitive yeast mutants S.
cerevisiae strain CY162, G19 and Axt3K. Transformed cells of G19
and Axt3K with pYES vector with cDNA inserts showed enhance
tolerance than those with empty pYes vector. Sequencing of the
cDNA inserts revealed that they encode for the putative proteins with
the sequence homologous to rice putative protein PROLM24
(Os06g31070), a prolamin precursor. Expression of this cDNA did
not affect yeast growth in absence of salt. Axt3k and G19 strains
expressing the PROLM24 were able to grow upto 400 mM and 600
mM of NaCl respectively. Similarly, Axt3k mutant with PROLM24
expression showed comparatively higher growth rate in the medium
with excess LiCl (50 mM). The observation that expression of
PROLM24 rescued the salt sensitive phenotypes of G19 and Axt3k
indicates the existence of a regulatory system that ameliorates the
effect of salt stress in the transformed yeast mutants. However, the
exact function of the cDNA sequence, which shows partial sequence
homology to yeast UTR1 is not clear. Although UTR1 involved in
ferrous uptake and iron homeostasis in yeast cells, there is no
evidence to prove its role in Na+ homeostasis in yeast cells. Absence
of transmembrane regions in Os06g31070 protein indicates that salt
tolerance is achieved not through the direct functional
complementation of the mutant genes but through an alternative
mechanism.
Abstract: This work aims to investigate a potential of
microalgae for utilizing industrial wastewater as a cheap nutrient for
their growth and oil accumulation. Wastewater was collected from
the effluent ponds of agro-industrial factories (cassava and ethanol
production plants). Only 2 microalgal strains were isolated and
identified as Scenedesmus quadricauda and Chlorella sp.. However,
only S. quadricauda was selected to cultivate in various wastewater
concentrations (10%, 20%, 40%, 60%, 80% and 100%). The highest
biomass obtained at 6.6×106 and 6.27×106 cells/ml when 60%
wastewater was used in flask and photo-bioreactor. The cultures gave
the highest lipid content at 18.58 % and 42.86% in cases of S.
quadricauda and S. obliquus. In addition, under salt stress (1.0 M
NaCl), S. obliquus demonstrated the highest lipid content at 50%
which was much more than the case of no NaCl adding. However, the
concentration of NaCl does not affect on lipid accumulation in case
of S. quadricauda.
Abstract: Protective clothing limits heat transfer and hampers
task performance due to the increased weight. Militarism protective
clothing enables humans to operate in adverse environments. In the
selection and evaluation of militarism protective clothing attention
should be given to heat strain, ergonomic and fit issues next to the
actual protection it offers.
Fifty Male healthy subjects participated in the study. The subjects
were dressed in shorts, T-shirts, socks, sneakers and four deferent
kinds of militarism protective clothing such as CS, CSB, CS with
NBC protection and CS with NBC- protection added.
Ergonomically and psychological strains of every four cloths were
investigated on subjects by walking on a treadmill (7km/hour) with a
19.7 kg backpack. As a result of these tests were showed that, the
highest heart rate was found wearing the NBC-protection added
outfit, the highest temperatures were observed wearing NBCprotection
added, followed by respectively CS with NBC protection,
CSB and CS and the highest value for thermal comfort (implying
worst thermal comfort) was observed wearing NBC-protection
added.
Abstract: Pentachlorophenol (PCP) is a polychlorinated
aromatic compound that is widespread in industrial effluents and is
considered to be a serious pollutant. Among the variety of industrial
effluents encountered, effluents from tanning industry are very
important and have a serious pollution potential. PCP is also formed
unintentionally in effluents of paper and pulp industries. It is highly
persistent in soils and is lethal to a wide variety of beneficial
microorganisms and insects, human beings and animals. The natural
processes that breakdown toxic chemicals in the environment have
become the focus of much attention to develop safe and environmentfriendly
deactivation technologies. Microbes and plants are among
the most important biological agents that remove and degrade waste
materials to enable their recycling in the environment. The present
investigation was carried out with the aim of developing a microbial
system for bioremediation of PCP polluted soils. A number of plant
species were evaluated for their ability to tolerate different
concentrations of pentachlorophenol (PCP) in the soil. The
experiment was conducted for 30 days under pot culture conditions.
The toxic effect of PCP on plants was studied by monitoring seed
germination, plant growth and biomass. As the concentration of PCP
was increased to 50 ppm, the inhibition of seed germination, plant
growth and biomass was also increased. Although PCP had a
negative effect on all plant species tested, maize and groundnut
showed the maximum tolerance to PCP. Other tolerating crops
included wheat, safflower, sunflower, and soybean. From the
rhizosphere soil of the tolerant seedlings, as many as twenty seven
PCP tolerant bacteria were isolated. From soybean, 8; sunflower, 3;
safflower 8; maize 2; groundnut and wheat, 3 each isolates were
made. They were screened for their PCP degradation potentials.
HPLC analyses of PCP degradation revealed that the isolate MAZ-2
degraded PCP completely. The isolate MAZ-1 was the next best
isolate with 90 per cent PCP degradation. These strains hold promise
to be used in the bioremediation of PCP polluted soils.
Abstract: Competitive relationships among Bradyrhizobium
japonicum USDA serogroup 123, 122 and 138 were screened versus
the standard commercial soybean variety Williams and two
introductions P1 377578 "671" in a field trial. Displacement of strain
123 by an effective strain should improved N2 fixation. Root nodules
were collected and strain occupancy percentage was determined
using strain specific fluorescent antibodies technique. As anticipated
the strain USDA 123 dominated 92% of nodules due to the high
affinity between the host and the symbiont. This dominance was
consistent and not changed materially either by inoculation practice
or by introducing new strainan. The interrelationship between the
genotype Williams and serogroup 122 & 138 was found very weak
although the cell density of the strain in the rhizosphere area was
equal. On the other hand, the nodule occupancy of genotypes 671 and
166 with rhizobia serogroup 123 was almost diminished to zero. .
The data further exhibited that the genotypes P1 671 and P1 166 have
high affinity to colonize with strains 122 and 138 whereas Williams
was highly promiscuous to strain 123.
Abstract: The complex structure of lignocellulose leads to great
difficulties in converting it to fermentable sugars for the ethanol
production. The major hydrolysis impediments are the crystallinity of
cellulose and the lignin content. To improve the efficiency of
enzymatic hydrolysis, microbial pretreatment of corncob was
investigated using two bacterial strains of Bacillus subtilis A 002 and
Cellulomonas sp. TISTR 784 (expected to break open the crystalline
part of cellulose) and lignin-degrading fungus, Phanerochaete
sordida SK7 (expected to remove lignin from lignocellulose). The
microbial pretreatment was carried out with each strain under its
optimum conditions. The pretreated corncob samples were further
hydrolyzed to produce reducing glucose with low amounts of
commercial cellulase (25 U·g-1 corncob) from Aspergillus niger. The
corncob samples were determined for composition change by X-ray
diffraction (XRD), Fourier transform infrared spectroscopy (FTIR),
and scanning electron microscope (SEM). According to the results,
the microbial pretreatment with fungus, P. sordida SK7 was the most
effective for enhancing enzymatic hydrolysis, approximately, 40%
improvement.
Abstract: The aim of this study was to screen for
microorganism that able to utilize 3-N-trimethylamino-1-propanol
(homocholine) as a sole source of carbon and nitrogen. The aerobic
degradation of homocholine has been found by a gram-positive
Rhodococcus sp. bacterium isolated from soil. The isolate was
identified as Rhodococcus sp. strain A4 based on the phenotypic
features, physiologic and biochemical characteristics, and
phylogenetic analysis. The cells of the isolated strain grown on both
basal-TMAP and nutrient agar medium displayed elementary
branching mycelia fragmented into irregular rod and coccoid
elements. Comparative 16S rDNA sequencing studies indicated that
the strain A4 falls into the Rhodococcus erythropolis subclade and
forms a monophyletic group with the type-strains of R. opacus, and
R. wratislaviensis. Metabolites analysis by capillary electrophoresis,
fast atom bombardment-mass spectrometry, and gas
chromatography- mass spectrometry, showed trimethylamine (TMA)
as the major metabolite beside β-alanine betaine and
trimethylaminopropionaldehyde. Therefore, the possible degradation
pathway of trimethylamino propanol in the isolated strain is through
consequence oxidation of alcohol group (-OH) to aldehyde (-CHO)
and acid (-COOH), and thereafter the cleavage of β-alanine betaine
C-N bonds yielded trimethylamine and alkyl chain.
Abstract: There are many issues that affect modeling and designing real-time databases. One of those issues is maintaining consistency between the actual state of the real-time object of the external environment and its images as reflected by all its replicas distributed over multiple nodes. The need to improve the scalability is another important issue. In this paper, we present a general framework to design a replicated real-time database for small to medium scale systems and maintain all timing constrains. In order to extend the idea for modeling a large scale database, we present a general outline that consider improving the scalability by using an existing static segmentation algorithm applied on the whole database, with the intent to lower the degree of replication, enables segments to have individual degrees of replication with the purpose of avoiding excessive resource usage, which all together contribute in solving the scalability problem for DRTDBS.
Abstract: The full length mitochondrial small subunit ribosomal
(mt-rns) gene has been characterized for Ophiostoma novo-ulmi
subspecies americana. The gene was also characterized for
Ophiostoma ulmi and a group II intron was noted in the mt-rns gene
of O. ulmi. The insertion in the mt-rns gene is at position S952 and it
is a group IIB1 intron that encodes a double motif LAGLIDADG
homing endonuclease from an open reading frame located within a
loop of domain III. Secondary structure models for the mt-rns RNA
of O. novo-ulmi subsp. americana and O. ulmi were generated to
place the intron within the context of the ribosomal RNA. The in vivo
splicing of the O.ul-mS952 group II intron was confirmed with
reverse transcription-PCR. A survey of 182 strains of Dutch Elm
Diseases causing agents showed that the mS952 intron was absent in
what is considered to be the more aggressive species O. novo-ulmi
but present in strains of the less aggressive O. ulmi. This observation
suggests that the O.ul-mS952 intron can be used as a PCR-based
molecular marker to discriminate between O. ulmi and O. novo-ulmi
subsp. americana.
Abstract: In order to enhance the knowledge of certain
phytochemical Algerian plants that are widely used in traditional
medicine and to exploit their therapeutic potential in modern
medicine, we have done a specific extraction of terpenes and
alkaloids from the leaves of Euphorbia granulata to evaluate the
antioxidant and antibacterial activity of this extracts. After the
extraction it was found that the terpene extract gave the highest yield
59.72% compared with alkaloids extracts.
The disc diffusion method was used to determine the antibacterial
activity against different bacterial strains: Escherichia coli
(ATCC25922), Pseudomonas aeruginosa (ATCC27853) and
Staphylococcus aureus (ATCC25923). All extracts have shown
inhibition of growth bacteria. The different zones of inhibition have
varied from (7 -10 mm) according to the concentrations of extract
used.
Testing the antiradical activity on DPPH-TLC plates indicated the
presence of substances that have potent anti-free radical. As against,
the BC-TLC revealed that only terpenes extract which was reacted
positively. These results can validate the importance of Euphorbia
granulata in traditional medicine.
Abstract: Perspective of food security in 21 century showed
shortage of food that production is faced to vital problem. Food
security strategy is applied longtime method to assess required food.
Meanwhile, nanotechnology revolution changes the world face.
Nanotechnology is adequate method utilize of its characteristics to
decrease environmental problems and possible further access to food
for small farmers. This article will show impact of production and
adoption of nanocrops on food security. Population is researchers of
agricultural research center of Esfahan province. The results of study
show that there was a relationship between uses, conversion,
distribution, and production of nanocrops, operative human
resources, operative circumstance, and constrains of usage of
nanocrops and food security. Multivariate regression analysis by
enter model shows that operative circumstance, use, production and
constrains of usage of nanocrops had positive impact on food security
and they determine in four steps 20 percent of it.
Abstract: In this paper, based on the coupled-mode and carrier rate equations, derivation of a dynamic model and numerically analysis of a MQW chirped DFB-SOA all-optical flip-flop is done precisely. We have analyzed the effects of strains of QW and MQW and cross phase modulation (XPM) on the dynamic response, and rise and fall times of the DFB-SOA all optical flip flop. We have shown that strained MQW active region in under an optimized condition into a DFB-SOA with chirped grating can improve the switching ON speed limitation in such a of the device, significantly while the fall time is increased. The values of the rise times for such an all optical flip-flop, are obtained in an optimized condition, areas tr=255ps.
Abstract: A. niger XP isolated from Vietnam produces very low amount of acidic phytase with optimal pH at 2.5 and 5.5. The phytase production of this strain was successfully improved through gene cloning and expression. A 1.4 - kb DNA fragment containing the coding region of the phyA gene was amplified by PCR and inserted into the expression vector pPICZαA with a signal peptide α- factor, under the control of AOX1 promoter. The recombined plasmid was transformed into the host strain P. pastoris KM71H and X33 by electroporation. Both host strains could efficiently express and secret phytase. The multicopy strains were screened for over expression of phytase. All the selected multicopy strains of P. pastoris X33 were examined for phytase activity, the maximum phytase yield of 1329 IU/ml was obtained after 4 days of incubation in medium BMM. The recombinant protein with MW of 97.4 KW showed to be the only one protein secreted in the culture broth. Multicopy transformant P. pastoris X33 supposed to be potential candidate for producing the commercial preparation of phytase.
Abstract: Newcastle Disease Virus (NDV), an avian
paramyxovirus, is a highly contagious, generalised virus disease of
domestic poultry and wild birds characterized by gastro-intestinal,
respiratory and nervous signs. In this study, it was shown that NDV
strain AF2240 and V4-UPM are cytolytic to Human Promyelocytic
Leukemia, HL60 and Human T-lymphoblastic Leukemia, CEM-SS
cells. Results from MTT cytolytic assay showed that CD50 for NDV
AF2240 against HL60 was 130 HAU and NDV V4-UPM against
HL60 and CEM-SS were 110.6 and 150.9 HAU respectively.
Besides, both strains were found to inhibit the proliferation of cells in
a dose dependent manner. The mode of cell death either by apoptosis
or necrosis was further analyzed using acridine orange and propidium
iodide (AO/PI) staining. Our results showed that both NDV strains
induced primarily apoptosis in treated cells at CD50 concentration. In
conclusion, both NDV strains caused cytolytic effects primarily via
apoptosis in leukemia cells.
Abstract: Intradiscal and intervertebral pressure transducers
were developed. They were used to map the pressures in the nucleus
and within the annulus of the human spinal segments. Their stressrelaxation
were recorded over a period of time for nucleus
pressure, applied load, and peripherial strain against time. The
results show that for normal discs, pressures in the nucleus are
viscoelastic in nature with the applied compressive load.
Mechanical strains which develop around the periphery of the
vertebral body are also viscoelastic with the applied compressive
load. Applied compressive load against time also shows viscoelastic
behavior. However, annulus does not respond viscoelastically with
the applied load. It showed a linear response to compressive loading.
Abstract: According to FDA (Food and Drug Administration of the United States), vinegar is definedas a sour liquid containing at least 4 grams acetic acid in 100 cubic centimeter (4% solution of acetic acid) of solution that is produced from sugary materials by alcoholic fermentation. In the base of microbial starters, vinegars could be contained of more than 50 types of volatile and aromatic substances that responsible for their sweet taste and smelling. Recently the vinegar industry has a great proportion in agriculture, food and microbial biotechnology. The acetic acid bacteria are from the family Acetobacteraceae. Regarding to the latest version of Bergy-s Mannual of Systematic Bacteriology that has categorized bacteria in the base of their 16s RNA differences, the most important acetic acid genera are included Acetobacter (genus I), Gluconacetobacter (genus VIII) and Gluconobacter (genus IX). The genus Acetobacter that is primarily used in vinegar manufacturing plants is a gram negative, obligate aerobe coccus or rod shaped bacterium with the size 0.6 - 0.8 X 1.0 - 4.0 μm, nonmotile or motile with peritrichous flagella and catalase positive – oxidase negative biochemically. Some strains are overoxidizer that could convert acetic acid to carbon dioxide and water.In this research one Acetobacter native strain with high acetic acid productivity was isolated from Iranian white – red cherry. We used two specific culture media include Carr medium [yeast extract, 3%; ethanol, 2% (v/v); bromocresol green, 0.002%; agar, 2% and distilled water, 1000 ml], Frateur medium [yeast extract, 10 g/l; CaCO3, 20 g/l; ethanol, 20 g/l; agar, 20 g/l and distilled water, 1000 ml] and an industrial culture medium. In addition to high acetic acid production and high growth rate, this strain had a good tolerance against ethanol concentration that was examined using modified Carr media with 5%, 7% and 9% ethanol concentrations. While the industrial strains of acetic acid bacteria grow in the thermal range of 28 – 30 °C, this strain was adapted for growth in 34 – 36 °C after 96 hours incubation period. These dramatic characteristics suggest a potential biotechnological strain in production of cherry vinegar with a sweet smell and different nutritional properties in comparison to recent vinegar types. The lack of growth after 24, 48 and 72 hours incubation at 34 – 36 °C and the growth after 96 hours indicates a good and fast thermal flexibility of this strain as a significant characteristic of biotechnological and industrial strains.
Abstract: Medical image data hiding has strict constrains such
as high imperceptibility, high capacity and high robustness.
Achieving these three requirements simultaneously is highly
cumbersome. Some works have been reported in the literature on
data hiding, watermarking and stegnography which are suitable for
telemedicine applications. None is reliable in all aspects. Electronic
Patient Report (EPR) data hiding for telemedicine demand it blind
and reversible. This paper proposes a novel approach to blind
reversible data hiding based on integer wavelet transform.
Experimental results shows that this scheme outperforms the prior
arts in terms of zero BER (Bit Error Rate), higher PSNR (Peak Signal
to Noise Ratio), and large EPR data embedding capacity with
WPSNR (Weighted Peak Signal to Noise Ratio) around 53 dB,
compared with the existing reversible data hiding schemes.
Abstract: The Swine flu outbreak in humans is due to a new
strain of influenza A virus subtype H1N1 that derives in part from
human influenza, avian influenza, and two separated strains of swine
influenza. It can be transmitted from human to human. A
mathematical model for the transmission of Swine flu is developed in
which the human populations are divided into two classes, the risk
and non-risk human classes. Each class is separated into susceptible,
exposed, infectious, quarantine and recovered sub-classes. In this
paper, we formulate the dynamical model of Swine flu transmission
and the repetitive contacts between the people are also considered.
We analyze the behavior for the transmission of this disease. The
Threshold condition of this disease is found and numerical results are
shown to confirm our theoretical predictions.
Abstract: Let G be a graph of order n, and let k 2 and m 0 be two integers. Let h : E(G) [0, 1] be a function. If e∋x h(e) = k holds for each x V (G), then we call G[Fh] a fractional k-factor of G with indicator function h where Fh = {e E(G) : h(e) > 0}. A graph G is called a fractional (k,m)-deleted graph if there exists a fractional k-factor G[Fh] of G with indicator function h such that h(e) = 0 for any e E(H), where H is any subgraph of G with m edges. In this paper, it is proved that G is a fractional (k,m)-deleted graph if (G) k + m + m k+1 , n 4k2 + 2k − 6 + (4k 2 +6k−2)m−2 k−1 and max{dG(x), dG(y)} n 2 for any vertices x and y of G with dG(x, y) = 2. Furthermore, it is shown that the result in this paper is best possible in some sense.