Application of Fluorescent Pseudomonads Inoculant Formulations on Vigna mungo through Field Trial
Vermiculite was used to develop inorganic
carrier-based formulations of fluorescent pseudomonad strains
R62 and R81. The effect of bio-inoculation of fluorescent
pseudomonad strains R62 and R81 (plant growth promoting
and biocontrol agent) on growth responses of Vigna-mungo
under field condition was enumerated. The combined bioinoculation
of these two organisms in a formuation increased
the pods yield by 300% in comparison to the control crop.
There was also significant increment in the other plant growth
responses such as dry root weight, dry shoot weight, shoot
length and number of branches per plant.
[1] Alagawadi, A.R., Gaur, A.C., 1988. Associative effect of Rhizobium
and phosphate-solubilizing bacteria on the yield and nutrient uptake
of chickpea. Plant Soil 105, 241-246.
[2] Bashan, Y., 1998. Inoculants of plant growth promoting bacteria
for use in agriculture. Biotechnological Advances 16, 729-770.
[3] Bajpai, P.D., Sundara Rao, W.V.B., 1971. Phosphate solubilizing
bacteria Part III. Soil inoculation with phorous solubilizing bacteria.
Soil. Sci. Plant. Nutr. 17, 46-52.
[4] Bashan,Y., Levanony, H., 1988. Adsorption of the rhizosphere
bacterium Azospirillum brusilense Cd to soil, sand and peat particles.
General. Microbiology 134, 1811-1820.
[5] Bennett, J.W., Lane, S.D., 1992. The potential role of Trichoderma
viride in the integrated control of Botrytis fabae. Mycologist 6, 199-
201.
[6] Beck, D. P., 1991. Suitability of charcoal amended mineral soil as
carrier for rhizobium inoculants. Soil Biology & Biochemistry 23,
41-44.
[7] Chet, I., 1987. TrichodermaÔÇöapplication, mode of action and
potential as a biocontrol agent of soil borne plant pathogenic fungi.
In: Chet, I. (Ed.), Innovative Approaches to Plant Disease Control,
John Wiley and Sons, New York, pp. 137-160.
[8] DeFreitas, J.R., Germide, J.J., 1992. Growth promotion of winter
wheat by fluorescent pseudomonas under growth chamber
conditions. Soil Biology & Biochemistry 24, 1127-1135.
[9] Dorosinsky, L.M., Kadyrov, A.A., 1975. Effect of inoculation of
nitrogen fixation by chickpea, its crop and content of protein.
Microbiologiya 44, 1103-1106.
[10] Glick, B. R., 1995. The enhancement of plant growth by free-living
bacteria. Can J. Microbiol 41, 109-117.
[11] Gaur, R., 2003. Diversity of DAPG and ACC deaminase producing
rhizobacteria from wheat. Ph.D. thesis, Department of Microbiology,
GB Pantnagar University of Ariculture & Technology, Uttar Pradesh,
India.
[12] Gaur, R., Shani, N., Kawaljeet, K., Johri, B.N., Rossi, P., Aragno,
M., 2004. Diacetylphloroglucinol-producing pseudomonads do not
influence AM fungi in wheat rhizosphere. Current Science 86, 453-
457.
[13] Herandez, L.G., Hill, G.S., 1983. Effect of plant population and
inoculation on yield and yield components of chickpea (Cicer
arietinum). Proc. Agron. Soc. N.Z. 13, 75-79.
[14] Gaur, A.C., Ostwal, K.P., Mathur, R.S., 1980. Save super phosphate
by using phosphate-solubilizing cultures and rock phosphate. Kheti
32, 23-25.
[15] Gupta, S.C., Namdeo, S.L., 1997. Effect of Rhizobium, phosphate
solubilizing bacteria and FYM on nodulation, grain yield and quality
of chickpea. Indian J. Pulses. Res. 10, 171-174.
[16] Johri, B.N., Sharma, A., Sharma, A.K., Glick, B.R., 2003. Plant
growth-promoting bacterium Pseudomonas sp. strain GRP3
influences iron acquisition in mung bean (Vigna radiata L. Wilzeck).
Soil Biology & Biochemistry 35, 887-894.
[17] Jain, P.C., Kushawaha, P.S., Dhakal, U.S., Khan, H., Trivedi, S.M.,
1999. Response of chickpea (cicer arietinum L.) to phosphorus and
biofertilzier. Legume. Res. 22, 241-244.
[18] King, J.V., 1948. The mineral requrements for fluorescin production.
Can. J. Res 26c, 514-519.
[19] Kloepper, J. W., Schroth, M. N., 1981. Development of powder
formulation of rhizobacteria for inoculation of potato seed pieces.
Phytopathology 71, 590-592.
[20] Khurana, A.S., Sharma, P., 2000. Effect of dual inoculation of
phosphate solubilizing bacteria, Bradyrhizobium sp. (cicer) and
phosphorus on nitrogen fixation and yield of chickpea. Indian J.
Pulses. Res. 13, 66-67.
[21] Kumar, R.N., Mukerji, K.G., 1996. Integrated disease
managementfuture perspectives. In: Mukerji, K.G., Mathur, B.,
Chamola, B.P., Chitralekha, C. (Eds.), Advances in Botany, APH
publishing Corp, New Delhi, pp. 335-347.
[22] Meyer, J.M., Abdallah, M.A., 1978. The fluorescent pigment of
Pseudomonas fluorescens: Biosynthesis, purification and
physiochemical properties. Journal General. Microbiology 107, 319-
328.
[23] Mukhopadhyay, A.N., Brahmbatt, A., Patel, G.J., 1986. Trichoderma
harzianum: a potential biocontrol agent for tobacco damping-off.
Tobacco. Res. 12, 26-35.
[24] Nair, N. G., Fahy, P.C., 1976. Commercial application of biological
control of mushroom bacteria blotch. Australian Journal Agriculture
Research 27, 415-422.
[25] Nelson, L. M., 2004. Plant growth promoting rhizobacteria (PGPR):
Prospects for new inoculants. Plant Management Network. Doi:
10.1094/CM-2004-0301-05-RV.
[26] Rudresha,D.L., Shivaprakasha, M.K., Prasad, R.D.., 2005. Effect of
combined application of Rhizobium, phosphate solubilizing
bacterium and Trichoderma spp. on growth, nutrient uptake and yield
of chickpea (Cicer aritenium L.) Applied Soil Ecology 28 139-146.
[27] Rice, W.A., Olsen, P.E., 1988. Soil inoculants for alfalfa grown on
moderately acid soil. Comm. Soil Sci. Plant Anal. 19, 947-956.
[28] Roesti, D., Gaur, R., Johri, B.N., Imfeld, G., Sharma, S., Kwaljeet,
K., Aragno, M., 2006. Plant growth stage, fertilizer management and
bio-inoculation of arbuscular mycorrhizal fungi and plant growth
promoting rhizobacteria affect the rhizobacterial community structure
in rain-fed wheat fields. Soil Biology & Biochemistry 38, 1111-1120.
[29] Podile, A. R., Kishore, G. K., 2006. Plant Growth Promoting
Rhizobacteria, Plant Associated Bacteria 3, 195-230.
[30] Papavizas, G.C., 1985. Trichoderma and Gliocladium: biology,
ecology and potential for biocontrol. Ann. Rev. Phytopathol. 23, 23-
54.
[31] Pierson, E.A., Weller, D.M., 1994. Use of mixtures of fluorescent
pseudomonas to supress take-all and improve the growth of wheat.
Phytopathology 84, 940-947.
[32] Paau, A.S., 1988. Formulation useful in applying beneficial
microorganisms to seeds. Recent Trends in Biotechnology 6, 276-
279.
[33] Suslow, T. V., Schroth, M. N., 1982. Rhizobacteria of sugar
beets;Effect of seed application and root colonization in yield.
Phytopathology 72, 199-206.
[34] The fertilizer (control) order 1985 (As ammended upto June 2006).
The Fertilizer Association of India. (On line)
http://www.dacnet.nic.in/ncof/quality_standards.htm/ (June 2006).
[35] Voisard, C., Keel, C., Hass, D. and Defago, G., 1989. Cyanide
production by Pseudomonas fluorescens suppress black root rot of
tobacco under gnotobiotic conditions. European Molecular Biology
Organization Journal 8, 351-358.
[36] Vidhyasekaran, P., Muthamilan,M., 1995. Development of
formulation of fluorescent pseudomonas fluorescens for control of
chickpea wilt. Plant Disease 79, 782-786.
[37] Vidhyasekaran, P., Sethuraman, K., Rajappan, K., Vasumathi, K.,
1997a. Powder Formulations of Pseudomonas fluoresces to Control
Pigeon pea Wilt, Biological control 8, 166-171.
[38] Vidhyasekaran, P., Rabindran, R., Muthamilan, M., Nayar, K.,
Rajappan, K., Subramanian N., Vasumathi, K., 1997b. Development
of a powder formulation of Pseudomonas fluorescens for contol of
rice blast. Plant Pathology 46 (3), 291-297.
[1] Alagawadi, A.R., Gaur, A.C., 1988. Associative effect of Rhizobium
and phosphate-solubilizing bacteria on the yield and nutrient uptake
of chickpea. Plant Soil 105, 241-246.
[2] Bashan, Y., 1998. Inoculants of plant growth promoting bacteria
for use in agriculture. Biotechnological Advances 16, 729-770.
[3] Bajpai, P.D., Sundara Rao, W.V.B., 1971. Phosphate solubilizing
bacteria Part III. Soil inoculation with phorous solubilizing bacteria.
Soil. Sci. Plant. Nutr. 17, 46-52.
[4] Bashan,Y., Levanony, H., 1988. Adsorption of the rhizosphere
bacterium Azospirillum brusilense Cd to soil, sand and peat particles.
General. Microbiology 134, 1811-1820.
[5] Bennett, J.W., Lane, S.D., 1992. The potential role of Trichoderma
viride in the integrated control of Botrytis fabae. Mycologist 6, 199-
201.
[6] Beck, D. P., 1991. Suitability of charcoal amended mineral soil as
carrier for rhizobium inoculants. Soil Biology & Biochemistry 23,
41-44.
[7] Chet, I., 1987. TrichodermaÔÇöapplication, mode of action and
potential as a biocontrol agent of soil borne plant pathogenic fungi.
In: Chet, I. (Ed.), Innovative Approaches to Plant Disease Control,
John Wiley and Sons, New York, pp. 137-160.
[8] DeFreitas, J.R., Germide, J.J., 1992. Growth promotion of winter
wheat by fluorescent pseudomonas under growth chamber
conditions. Soil Biology & Biochemistry 24, 1127-1135.
[9] Dorosinsky, L.M., Kadyrov, A.A., 1975. Effect of inoculation of
nitrogen fixation by chickpea, its crop and content of protein.
Microbiologiya 44, 1103-1106.
[10] Glick, B. R., 1995. The enhancement of plant growth by free-living
bacteria. Can J. Microbiol 41, 109-117.
[11] Gaur, R., 2003. Diversity of DAPG and ACC deaminase producing
rhizobacteria from wheat. Ph.D. thesis, Department of Microbiology,
GB Pantnagar University of Ariculture & Technology, Uttar Pradesh,
India.
[12] Gaur, R., Shani, N., Kawaljeet, K., Johri, B.N., Rossi, P., Aragno,
M., 2004. Diacetylphloroglucinol-producing pseudomonads do not
influence AM fungi in wheat rhizosphere. Current Science 86, 453-
457.
[13] Herandez, L.G., Hill, G.S., 1983. Effect of plant population and
inoculation on yield and yield components of chickpea (Cicer
arietinum). Proc. Agron. Soc. N.Z. 13, 75-79.
[14] Gaur, A.C., Ostwal, K.P., Mathur, R.S., 1980. Save super phosphate
by using phosphate-solubilizing cultures and rock phosphate. Kheti
32, 23-25.
[15] Gupta, S.C., Namdeo, S.L., 1997. Effect of Rhizobium, phosphate
solubilizing bacteria and FYM on nodulation, grain yield and quality
of chickpea. Indian J. Pulses. Res. 10, 171-174.
[16] Johri, B.N., Sharma, A., Sharma, A.K., Glick, B.R., 2003. Plant
growth-promoting bacterium Pseudomonas sp. strain GRP3
influences iron acquisition in mung bean (Vigna radiata L. Wilzeck).
Soil Biology & Biochemistry 35, 887-894.
[17] Jain, P.C., Kushawaha, P.S., Dhakal, U.S., Khan, H., Trivedi, S.M.,
1999. Response of chickpea (cicer arietinum L.) to phosphorus and
biofertilzier. Legume. Res. 22, 241-244.
[18] King, J.V., 1948. The mineral requrements for fluorescin production.
Can. J. Res 26c, 514-519.
[19] Kloepper, J. W., Schroth, M. N., 1981. Development of powder
formulation of rhizobacteria for inoculation of potato seed pieces.
Phytopathology 71, 590-592.
[20] Khurana, A.S., Sharma, P., 2000. Effect of dual inoculation of
phosphate solubilizing bacteria, Bradyrhizobium sp. (cicer) and
phosphorus on nitrogen fixation and yield of chickpea. Indian J.
Pulses. Res. 13, 66-67.
[21] Kumar, R.N., Mukerji, K.G., 1996. Integrated disease
managementfuture perspectives. In: Mukerji, K.G., Mathur, B.,
Chamola, B.P., Chitralekha, C. (Eds.), Advances in Botany, APH
publishing Corp, New Delhi, pp. 335-347.
[22] Meyer, J.M., Abdallah, M.A., 1978. The fluorescent pigment of
Pseudomonas fluorescens: Biosynthesis, purification and
physiochemical properties. Journal General. Microbiology 107, 319-
328.
[23] Mukhopadhyay, A.N., Brahmbatt, A., Patel, G.J., 1986. Trichoderma
harzianum: a potential biocontrol agent for tobacco damping-off.
Tobacco. Res. 12, 26-35.
[24] Nair, N. G., Fahy, P.C., 1976. Commercial application of biological
control of mushroom bacteria blotch. Australian Journal Agriculture
Research 27, 415-422.
[25] Nelson, L. M., 2004. Plant growth promoting rhizobacteria (PGPR):
Prospects for new inoculants. Plant Management Network. Doi:
10.1094/CM-2004-0301-05-RV.
[26] Rudresha,D.L., Shivaprakasha, M.K., Prasad, R.D.., 2005. Effect of
combined application of Rhizobium, phosphate solubilizing
bacterium and Trichoderma spp. on growth, nutrient uptake and yield
of chickpea (Cicer aritenium L.) Applied Soil Ecology 28 139-146.
[27] Rice, W.A., Olsen, P.E., 1988. Soil inoculants for alfalfa grown on
moderately acid soil. Comm. Soil Sci. Plant Anal. 19, 947-956.
[28] Roesti, D., Gaur, R., Johri, B.N., Imfeld, G., Sharma, S., Kwaljeet,
K., Aragno, M., 2006. Plant growth stage, fertilizer management and
bio-inoculation of arbuscular mycorrhizal fungi and plant growth
promoting rhizobacteria affect the rhizobacterial community structure
in rain-fed wheat fields. Soil Biology & Biochemistry 38, 1111-1120.
[29] Podile, A. R., Kishore, G. K., 2006. Plant Growth Promoting
Rhizobacteria, Plant Associated Bacteria 3, 195-230.
[30] Papavizas, G.C., 1985. Trichoderma and Gliocladium: biology,
ecology and potential for biocontrol. Ann. Rev. Phytopathol. 23, 23-
54.
[31] Pierson, E.A., Weller, D.M., 1994. Use of mixtures of fluorescent
pseudomonas to supress take-all and improve the growth of wheat.
Phytopathology 84, 940-947.
[32] Paau, A.S., 1988. Formulation useful in applying beneficial
microorganisms to seeds. Recent Trends in Biotechnology 6, 276-
279.
[33] Suslow, T. V., Schroth, M. N., 1982. Rhizobacteria of sugar
beets;Effect of seed application and root colonization in yield.
Phytopathology 72, 199-206.
[34] The fertilizer (control) order 1985 (As ammended upto June 2006).
The Fertilizer Association of India. (On line)
http://www.dacnet.nic.in/ncof/quality_standards.htm/ (June 2006).
[35] Voisard, C., Keel, C., Hass, D. and Defago, G., 1989. Cyanide
production by Pseudomonas fluorescens suppress black root rot of
tobacco under gnotobiotic conditions. European Molecular Biology
Organization Journal 8, 351-358.
[36] Vidhyasekaran, P., Muthamilan,M., 1995. Development of
formulation of fluorescent pseudomonas fluorescens for control of
chickpea wilt. Plant Disease 79, 782-786.
[37] Vidhyasekaran, P., Sethuraman, K., Rajappan, K., Vasumathi, K.,
1997a. Powder Formulations of Pseudomonas fluoresces to Control
Pigeon pea Wilt, Biological control 8, 166-171.
[38] Vidhyasekaran, P., Rabindran, R., Muthamilan, M., Nayar, K.,
Rajappan, K., Subramanian N., Vasumathi, K., 1997b. Development
of a powder formulation of Pseudomonas fluorescens for contol of
rice blast. Plant Pathology 46 (3), 291-297.
@article{"International Journal of Biological, Life and Agricultural Sciences:55586", author = "Sarma. M.V.R.K and Saharan. K and Prakash. A and Bisaria. V. S and Sahai.V", title = "Application of Fluorescent Pseudomonads Inoculant Formulations on Vigna mungo through Field Trial", abstract = "Vermiculite was used to develop inorganic
carrier-based formulations of fluorescent pseudomonad strains
R62 and R81. The effect of bio-inoculation of fluorescent
pseudomonad strains R62 and R81 (plant growth promoting
and biocontrol agent) on growth responses of Vigna-mungo
under field condition was enumerated. The combined bioinoculation
of these two organisms in a formuation increased
the pods yield by 300% in comparison to the control crop.
There was also significant increment in the other plant growth
responses such as dry root weight, dry shoot weight, shoot
length and number of branches per plant.", keywords = "Bio-inoculants formulation, Fluorescent
pseudomonad, Plant growth promotion activity.", volume = "3", number = "3", pages = "149-5", }
