Abstract: Phytases (myo-inositol hexakisphosphate
phosphohydrolases; EC 3.1.3.8) catalyze the hydrolysis of phytic acid
(myoinositol hexakisphosphate) to the mono-, di-, tri-, tetra-, and
pentaphosphates of myo-inositol and inorganic phosphate.
Therrmophilic bacteria isolated from water sampled from hot spring.
About 120 isolates of bacteria were successfully isolated form hot
spring water sample and tested for extracellular phytase producing.
After 5 passages of the screening on the PSM media, 4 isolates were
found stable in producing phytase enzyme. The 16s RDNA
sequencing for identification of bacteria using molecular technique
revealed that all isolates those positive in phytase producing are
belong to Geobacillus spp. And Anoxybacillus spp. Anoxybacillus
rupiensis UniSZA-7 were identified for their carbon source utilization
using Phenotype Microarray Plate of Biolog and found they utilize
several kind of carbon source provided.
Abstract: Newcastle Disease Virus (NDV), an avian
paramyxovirus, is a highly contagious, generalised virus disease of
domestic poultry and wild birds characterized by gastro-intestinal,
respiratory and nervous signs. In this study, it was shown that NDV
strain AF2240 and V4-UPM are cytolytic to Human Promyelocytic
Leukemia, HL60 and Human T-lymphoblastic Leukemia, CEM-SS
cells. Results from MTT cytolytic assay showed that CD50 for NDV
AF2240 against HL60 was 130 HAU and NDV V4-UPM against
HL60 and CEM-SS were 110.6 and 150.9 HAU respectively.
Besides, both strains were found to inhibit the proliferation of cells in
a dose dependent manner. The mode of cell death either by apoptosis
or necrosis was further analyzed using acridine orange and propidium
iodide (AO/PI) staining. Our results showed that both NDV strains
induced primarily apoptosis in treated cells at CD50 concentration. In
conclusion, both NDV strains caused cytolytic effects primarily via
apoptosis in leukemia cells.