Restriction Specificity of Some Soybean Genotypes to Bradyrhizobium japonicum Serogrous
Competitive relationships among Bradyrhizobium
japonicum USDA serogroup 123, 122 and 138 were screened versus
the standard commercial soybean variety Williams and two
introductions P1 377578 "671" in a field trial. Displacement of strain
123 by an effective strain should improved N2 fixation. Root nodules
were collected and strain occupancy percentage was determined
using strain specific fluorescent antibodies technique. As anticipated
the strain USDA 123 dominated 92% of nodules due to the high
affinity between the host and the symbiont. This dominance was
consistent and not changed materially either by inoculation practice
or by introducing new strainan. The interrelationship between the
genotype Williams and serogroup 122 & 138 was found very weak
although the cell density of the strain in the rhizosphere area was
equal. On the other hand, the nodule occupancy of genotypes 671 and
166 with rhizobia serogroup 123 was almost diminished to zero. .
The data further exhibited that the genotypes P1 671 and P1 166 have
high affinity to colonize with strains 122 and 138 whereas Williams
was highly promiscuous to strain 123.
[1] Amerger, N and Lobreau, J.P. (1982). Quantitive study of nodulation
competitiveness in Rhizobium strains. Appl. Environ. Microbiol. 44: 583
- 588.
[2] Ham, G.E. (1980). Ineractions of Glycine max. and Rhizobium
japonicum. In.R.J. Summerfield and A.H. Bunting (ed.) Advances in
Legume Science. Royal Botinical Gardens. Kew, UK.: 289 - 296.
[3] Cregan, B.E. and van Berkum, P. (1984). Genetics of nitrogen
metabolism and physiological / biochemical selection for increased grain
crop productivity. Theor. Appl. Genet. 67: 97 - 111.
[4] Lohrke, S,M.; Orf, J.H.; Martinez-Romero, E. and Sadowsky, M.J.
(1995). Host control restriction of nodulation by Bradyrhizobium
japonicum strains in serogroup 110. Appl. Environ. Microbiol., 61 (6):
2378 - 2383.
[5] Ham, G.E.; Frederick, L.R. and Anderson, I.C. (1971). Serogroups of
Rhizobium japonicum in soybean nodules. Agron. J. 63: 69 - 72.
[6] Kvien, C.S.; Ham, J.E. and Lambert, J.W. (1981). Recovery of
introduced Rhizobium japonicum strains by soybean genotypes. Agron J.
73: 900 - 905.
[7] Moawad, H.A.; Ellis, W.R. and Schmidt, E.L. (1984). Rhizosphere
response as a factor of competition among three serogroups of
indigenious Rhizobium japonicum for nodulation of field grown
soybeans. Appl. Environ. Microbiol. 47: 607 - 612.
[8] Ham, G.E. (1976). Competition among strains of rhizobia. In. I.D. Hill
(ed.). World Soybean Research. Institute Printers and Puplishers,
Danville,IL.
[9] Cregan, P.B. and Keyser, H.H. (1986). Host restrication of nodulation
by Bradyrhizobium japonicum strain USDA 123 in soybean. Crop
Science, 26: 911 - 916.
[10] Weiser, G.C.; Skipper, H.D. and Wollum, A.G. 11 (1990). Exclusion of
inefficient Bradyrhizobium japonicum serogroups by soybean
genotypes. Plant and Soil, 121 (1): 99 - 105.
[11] Ferrey, M.L.; Graham, P.H. and Russelle, M.P. (1994). Nodulation
efficiency of Bradyrhizobium japonicum strains with genotypes of
soybean varying in the ability to restrict nodulation. Canadian Journal of
Microbiolgy, 40 (6): 456 - 460.
[12] Schmidt, E.L.; Bankole, R.O. and Bohlool, B.B. (1968). Fluorscentantibody
approach to study rhizobia in soil. J. Bacteriol. 95: 1987 -
1992.
[13] Vincent, J.M. (1970). A Manual for The Practical Study of The Rootnodule
Bacteria. Int. Biological Programme Handbook no. 15. Blackwell
Scientific Puplication, Oxford, UK.
[14] Reyes, V.G. and Schmidt,E.L. (1981). Population of Rhizobium
japonicum associated with the surfaces of soil grown roots. Plant and
Soil, 61: 71 - 80.
[15] Pazdernik, D.L.; Vance, C.P.; Sadowsky, M.J.; Graham, P.H. and Orf,
J.H. (1997). A host- controlled, serogroup- specific, ineffectivenodulation
system in the Bradyrhizobium-soybean (Glycine max)
symbiosis. Molecular-Plant-Microbe-Interaction, 10 (8): 994 -1001.
[16] Caldwell, B.E. and Vest, G. (1970). Effect of Rhizobium japonicum
strains on soybean yields. Crop. Sci. 10: 19 - 21.
[17] Cregan P.B.; Keyser, H.H. and Sadowsky, M.J. (1989). Host plant
effects on nodulation and competitiveness of the Bradyrhizobium
japonicum serotype strains consisting serocluster 123. Appl. Environ.
Microbiol., 55 (10): 2532 - 2536.
[1] Amerger, N and Lobreau, J.P. (1982). Quantitive study of nodulation
competitiveness in Rhizobium strains. Appl. Environ. Microbiol. 44: 583
- 588.
[2] Ham, G.E. (1980). Ineractions of Glycine max. and Rhizobium
japonicum. In.R.J. Summerfield and A.H. Bunting (ed.) Advances in
Legume Science. Royal Botinical Gardens. Kew, UK.: 289 - 296.
[3] Cregan, B.E. and van Berkum, P. (1984). Genetics of nitrogen
metabolism and physiological / biochemical selection for increased grain
crop productivity. Theor. Appl. Genet. 67: 97 - 111.
[4] Lohrke, S,M.; Orf, J.H.; Martinez-Romero, E. and Sadowsky, M.J.
(1995). Host control restriction of nodulation by Bradyrhizobium
japonicum strains in serogroup 110. Appl. Environ. Microbiol., 61 (6):
2378 - 2383.
[5] Ham, G.E.; Frederick, L.R. and Anderson, I.C. (1971). Serogroups of
Rhizobium japonicum in soybean nodules. Agron. J. 63: 69 - 72.
[6] Kvien, C.S.; Ham, J.E. and Lambert, J.W. (1981). Recovery of
introduced Rhizobium japonicum strains by soybean genotypes. Agron J.
73: 900 - 905.
[7] Moawad, H.A.; Ellis, W.R. and Schmidt, E.L. (1984). Rhizosphere
response as a factor of competition among three serogroups of
indigenious Rhizobium japonicum for nodulation of field grown
soybeans. Appl. Environ. Microbiol. 47: 607 - 612.
[8] Ham, G.E. (1976). Competition among strains of rhizobia. In. I.D. Hill
(ed.). World Soybean Research. Institute Printers and Puplishers,
Danville,IL.
[9] Cregan, P.B. and Keyser, H.H. (1986). Host restrication of nodulation
by Bradyrhizobium japonicum strain USDA 123 in soybean. Crop
Science, 26: 911 - 916.
[10] Weiser, G.C.; Skipper, H.D. and Wollum, A.G. 11 (1990). Exclusion of
inefficient Bradyrhizobium japonicum serogroups by soybean
genotypes. Plant and Soil, 121 (1): 99 - 105.
[11] Ferrey, M.L.; Graham, P.H. and Russelle, M.P. (1994). Nodulation
efficiency of Bradyrhizobium japonicum strains with genotypes of
soybean varying in the ability to restrict nodulation. Canadian Journal of
Microbiolgy, 40 (6): 456 - 460.
[12] Schmidt, E.L.; Bankole, R.O. and Bohlool, B.B. (1968). Fluorscentantibody
approach to study rhizobia in soil. J. Bacteriol. 95: 1987 -
1992.
[13] Vincent, J.M. (1970). A Manual for The Practical Study of The Rootnodule
Bacteria. Int. Biological Programme Handbook no. 15. Blackwell
Scientific Puplication, Oxford, UK.
[14] Reyes, V.G. and Schmidt,E.L. (1981). Population of Rhizobium
japonicum associated with the surfaces of soil grown roots. Plant and
Soil, 61: 71 - 80.
[15] Pazdernik, D.L.; Vance, C.P.; Sadowsky, M.J.; Graham, P.H. and Orf,
J.H. (1997). A host- controlled, serogroup- specific, ineffectivenodulation
system in the Bradyrhizobium-soybean (Glycine max)
symbiosis. Molecular-Plant-Microbe-Interaction, 10 (8): 994 -1001.
[16] Caldwell, B.E. and Vest, G. (1970). Effect of Rhizobium japonicum
strains on soybean yields. Crop. Sci. 10: 19 - 21.
[17] Cregan P.B.; Keyser, H.H. and Sadowsky, M.J. (1989). Host plant
effects on nodulation and competitiveness of the Bradyrhizobium
japonicum serotype strains consisting serocluster 123. Appl. Environ.
Microbiol., 55 (10): 2532 - 2536.
@article{"International Journal of Biological, Life and Agricultural Sciences:55066", author = "H.K. Abd El-Maksoud and H.H. Keyser", title = "Restriction Specificity of Some Soybean Genotypes to Bradyrhizobium japonicum Serogrous", abstract = "Competitive relationships among Bradyrhizobium
japonicum USDA serogroup 123, 122 and 138 were screened versus
the standard commercial soybean variety Williams and two
introductions P1 377578 "671" in a field trial. Displacement of strain
123 by an effective strain should improved N2 fixation. Root nodules
were collected and strain occupancy percentage was determined
using strain specific fluorescent antibodies technique. As anticipated
the strain USDA 123 dominated 92% of nodules due to the high
affinity between the host and the symbiont. This dominance was
consistent and not changed materially either by inoculation practice
or by introducing new strainan. The interrelationship between the
genotype Williams and serogroup 122 & 138 was found very weak
although the cell density of the strain in the rhizosphere area was
equal. On the other hand, the nodule occupancy of genotypes 671 and
166 with rhizobia serogroup 123 was almost diminished to zero. .
The data further exhibited that the genotypes P1 671 and P1 166 have
high affinity to colonize with strains 122 and 138 whereas Williams
was highly promiscuous to strain 123.", keywords = "B. japonicum serogroups, Competition, Host
restriction, Soybean genotype.", volume = "4", number = "11", pages = "818-4", }
