Abstract: With getting older in the whole population, the
prevalence of stroke and its residual disability is getting higher and
higher recently in Taiwan. The functional electrical stimulation
cycling system (FESCS) is useful for hemiplegic patients. Because
that the muscle of stroke patients is under hybrid activation. The raw
electromyography (EMG) represents the residual muscle force of
stroke subject whereas the peak-to-peak of stimulus EMG indicates the
force enhancement benefiting from ES. It seems that EMG signals
could be used for a parameter of feedback control mechanism. So, we
design the feedback control protocol of FESCS, it includes
physiological signal recorder, FPGA biomedical module, DAC and
electrical stimulation circuit. Using the intensity of real-time EMG
signal obtained from patients, as a feedback control method for the
output voltage of FES-cycling system.
Abstract: Carbon tetrachloride (CCl4) is a well-known
hepatotoxin and exposure to this chemical is known to induce
oxidative stress and causes liver injury by the formation of free
radicals. Flacourtia indica commonly known as 'Baichi' has been
reported as an effective remedy for the treatment of a variety of
diseases. The objective of this study was to investigate the
hepatoprotective activity of aqueous extract of leaves of Flacourtia
indica against CCl4 induced hepatotoxicity. Animals were pretreated
with the aqueous extract of Flacourtia indica (250 & 500 mg/kg
body weight) for one week and then challenged with CCl4 (1.5 ml/kg
bw) in olive oil (1:1, v/v) on 7th day. Serum marker enzymes (ALP,
AST, ALT, Total Protein & Total Bilirubin) and TBARS level
(Marker for oxidative stress) were estimated in all the study groups.
Alteration in the levels of biochemical markers of hepatic damage
like AST, ALT, ALP, Total Protein, Total Bilirubin and lipid
peroxides (TBARS) were tested in both CCl4 treated and extract
treated groups. CCl4 has enhanced the AST, ALT, ALP and the
Lipid peroxides (TBARS) in liver. Treatment of aqueous extract of
Flacourtia indica leaves (250 & 500 mg/kg) exhibited a significant
protective effect by altering the serum levels of AST, ALT, ALP,
Total Protein, Total Bilirubin and liver TBARS. These biochemical
observations were supported by histopathological study of liver
sections. From this preliminary study it has been concluded that the
aqueous extract of the leaves of Flacourtia indica protects liver
against oxidative damages and could be used as an effective protector
against CCl4 induced hepatic damage. Our findings suggested that
Flacourtia indica possessed good hepatoprotective activity
Abstract: The field of biomedical materials plays an imperative
requisite and a critical role in manufacturing a variety of biological
artificial replacements in a modern world. Recently, titanium (Ti)
materials are being used as biomaterials because of their superior
corrosion resistance and tremendous specific strength, free- allergic
problems and the greatest biocompatibility compared to other
competing biomaterials such as stainless steel, Co-Cr alloys,
ceramics, polymers, and composite materials. However, regardless of
these excellent performance properties, Implantable Ti materials have
poor shear strength and wear resistance which limited their
applications as biomaterials. Even though the wear properties of Ti
alloys has revealed some improvements, the crucial effectiveness of
biomedical Ti alloys as wear components requires a comprehensive
deep understanding of the wear reasons, mechanisms, and techniques
that can be used to improve wear behavior. This review examines
current information on the effect of thermal and thermomechanical
processing of implantable Ti materials on the long-term prosthetic
requirement which related with wear behavior. This paper focuses
mainly on the evolution, evaluation and development of effective
microstructural features that can improve wear properties of bio
grade Ti materials using thermal and thermomechanical treatments.
Abstract: Many high-risk pathogens that cause disease in
humans are transmitted through various food items. Food-borne
disease constitutes a major public health problem. Assessment of the
quality and safety of foods is important in human health. Rapid and
easy detection of pathogenic organisms will facilitate precautionary
measures to maintain healthy food. The Polymerase Chain Reaction
(PCR) is a handy tool for rapid detection of low numbers of bacteria.
We have designed gene specific primers for most common food
borne pathogens such as Staphylococci, Salmonella and E.coli.
Bacteria were isolated from food samples of various food outlets and
identified using gene specific PCRs. We identified Staphylococci,
Salmonella and E.coli O157 using gene specific primers by rapid and
direct PCR technique in various food samples. This study helps us in
getting a complete picture of the various pathogens that threaten to
cause and spread food borne diseases and it would also enable
establishment of a routine procedure and methodology for rapid
identification of food borne bacteria using the rapid technique of
direct PCR. This study will also enable us to judge the efficiency of
present food safety steps taken by food manufacturers and exporters.
Abstract: Data mining incorporates a group of statistical
methods used to analyze a set of information, or a data set. It operates
with models and algorithms, which are powerful tools with the great
potential. They can help people to understand the patterns in certain
chunk of information so it is obvious that the data mining tools have
a wide area of applications. For example in the theoretical chemistry
data mining tools can be used to predict moleculeproperties or
improve computer-assisted drug design. Classification analysis is one
of the major data mining methodologies. The aim of thecontribution
is to create a classification model, which would be able to deal with a
huge data set with high accuracy. For this purpose logistic regression,
Bayesian logistic regression and random forest models were built
using R software. TheBayesian logistic regression in Latent GOLD
software was created as well. These classification methods belong to
supervised learning methods.
It was necessary to reduce data matrix dimension before construct
models and thus the factor analysis (FA) was used. Those models
were applied to predict the biological activity of molecules, potential
new drug candidates.
Abstract: Diffuse viral encephalitis may lack fever and other cardinal signs of infection and hence its distinction from other acute encephalopathic illnesses is challenging. Often, the EEG changes seen routinely are nonspecific and reflect diffuse encephalopathic changes only. The aim of this study was to use nonlinear dynamic mathematical techniques for analyzing the EEG data in order to look for any characteristic diagnostic patterns in diffuse forms of encephalitis.It was diagnosed on clinical, imaging and cerebrospinal fluid criteria in three young male patients. Metabolic and toxic encephalopathies were ruled out through appropriate investigations. Digital EEGs were done on the 3rd to 5th day of onset. The digital EEGs of 5 male and 5 female age and sex matched healthy volunteers served as controls.Two sample t-test indicated that there was no statistically significant difference between the average values in amplitude between the two groups. However, the standard deviation (or variance) of the EEG signals at FP1-F7 and FP2-F8 are significantly higher for the patients than the normal subjects. The regularisation dimension is significantly less for the patients (average between 1.24-1.43) when compared to the normal persons (average between 1.41-1.63) for the EEG signals from all locations except for the Fz-Cz signal. Similarly the wavelet dimension is significantly less (P = 0.05*) for the patients (1.122) when compared to the normal person (1.458). EEGs are subdued in the case of the patients with presence of uniform patterns, manifested in the values of regularisation and wavelet dimensions, when compared to the normal person, indicating a decrease in chaotic nature.
Abstract: An application of the highly biosensor based on pH-sensitive field immobilized urease for urea analysis was demo The main analytical characteristics of the bios determined; the conditions of urea measureme blood were optimized. A conceptual possibility biosensor for detection of urea concentratio patients suffering from renal insufficiency was sensitive and selective effect transistor and monstrated in this work. iosensor developed were ment in real samples of ility of application of the tion in blood serum of as shown.
Abstract: Information regarding early onset neonatal sepsis
(EONS) pathogens may vary between regions. Global perspectives
showed Group B Streptococcal (GBS) as the most common causative
pathogens, but the widespread use of intrapartum antibiotics has
changed the pathogens pattern towards gram negative
microorganisms, especially E. coli. Objective of this study is to
describe the pathogens isolated, to assess current treatment and risk
of EONS. Records of 899 neonates born in three General Hospitals
between 2009 until 2012 were retrospectively reviewed. Proven was
found in 22 (3%) neonates. The majority was isolated with gram
positive organisms, 17 (2.3%). All grams positive and most gram
negative organisms showed sensitivity to the tested antibiotics. Only
two rare gram negative organisms showed total resistant. Male was
possible risk of proven EONS. Although proven EONS remains
uncommon in Malaysia, nonetheless, the effect of intrapartum
antibiotics still required continuous surveillance.
Abstract: There is an urgent need to develop novel
Mycobacterium tuberculosis (Mtb) drugs that are active against drug
resistant bacteria but, more importantly, kill persistent bacteria. Our
study structured based on integrated analysis of metabolic pathways,
small molecule screening and similarity Search in PubChem
Database. Metabolic analysis approaches based on Unified weighted
used for potent target selection. Our results suggest that pantothenate
synthetase (panC) and and 3-methyl-2-oxobutanoate hydroxymethyl
transferase (panB) as a appropriate drug targets. In our study, we
used pantothenate synthetase because of existence inhibitors. We
have reported the discovery of new antitubercular compounds
through ligand based approaches using computational tools.
Abstract: This paper illustrates the use of a combined neural
network model for classification of electrocardiogram (ECG) beats.
We present a trainable neural network ensemble approach to develop
customized electrocardiogram beat classifier in an effort to further
improve the performance of ECG processing and to offer
individualized health care.
We process a three stage technique for detection of premature
ventricular contraction (PVC) from normal beats and other heart
diseases. This method includes a denoising, a feature extraction and a
classification. At first we investigate the application of stationary
wavelet transform (SWT) for noise reduction of the
electrocardiogram (ECG) signals. Then feature extraction module
extracts 10 ECG morphological features and one timing interval
feature. Then a number of multilayer perceptrons (MLPs) neural
networks with different topologies are designed.
The performance of the different combination methods as well as
the efficiency of the whole system is presented. Among them,
Stacked Generalization as a proposed trainable combined neural
network model possesses the highest recognition rate of around 95%.
Therefore, this network proves to be a suitable candidate in ECG
signal diagnosis systems. ECG samples attributing to the different
ECG beat types were extracted from the MIT-BIH arrhythmia
database for the study.
Abstract: A mathematical model of the respiratory system is
introduced in this study. Geometrical dimensions of the respiratory
system were used to compute the acoustic properties of the
respiratory system using the electro-acoustic analogy. The effect of
the geometrical proportions of the respiratory system is observed in
the paper.
Abstract: This paper presents the design of a ring-shaped tri-axial fore sensor that can be incorporated into the tip of a guidewire for use in minimally invasive surgery (MIS). The designed sensor comprises a ring-shaped structure located at the center of four cantilever beams. The ringdesign allows surgical tools to be easily passed through which largely simplified the integration process. Silicon nanowires (SiNWs) are used aspiezoresistive sensing elementsembeddedon the four cantilevers of the sensor to detect the resistance change caused by the applied load.An integration scheme with new designed guidewire tip structure having two coils at the distal end is presented. Finite element modeling has been employed in the sensor design to find the maximum stress location in order to put the SiNWs at the high stress regions to obtain maximum output. A maximum applicable force of 5 mN is found from modeling. The interaction mechanism between the designed sensor and a steel wire has been modeled by FEM. A linear relationship between the applied load on the steel wire and the induced stress on the SiNWs were observed.
Abstract: Knee joint forces are available by in vivo measurement
using an instrumented knee prosthesis for small to moderate knee
flexion but not for high flexion yet. We created a 2D mathematical
model of the lower limb incorporating several new features such as a
patello-femoral mechanism, a thigh-calf contact at high knee flexion
and co-contracting muscles' force ratio, then used it to determine knee
joint forces arising from high knee flexions in four kneeling
conditions: rising with legs in parallel, with one foot forward, with or
without arm use. With arms used, the maximum values of knee joint
force decreased to about 60% of those with arms not used. When rising
with one foot forward, if arms are not used, the forward leg sustains a
force as large as that sustained when rising with legs parallel.
Abstract: Cancer becomes one of the leading cause of death in
many countries over the world. Fourier-transform infrared (FTIR)
spectra of human lung cancer cells (A549) treated with PMF (natural
product extracted from PM 701) for different time intervals were
examined. Second derivative and difference method were taken in
comparison studies. Cesium (Cs) and Rubidium (Rb) nanoparticles in
PMF were detected by Energy Dispersive X-ray attached to Scanning
Electron Microscope SEM-EDX. Characteristic changes in protein
secondary structure, lipid profile and changes in the intensities of
DNA bands were identified in treated A549 cells spectra. A
characteristic internucleosomal ladder of DNA fragmentation was
also observed after 30 min of treatment. Moreover, the pH values
were significantly increases upon treatment due to the presence of Cs
and Rb nanoparticles in the PMF fraction. These results support the
previous findings that PMF is selective anticancer agent and can
produce apoptosis to A549 cells.
Abstract: Quality of 2D and 3D cross-sectional images produce
by Computed Tomography primarily depend upon the degree of
precision of primary and secondary X-Ray intensity detection.
Traditional method of primary intensity detection is apt to errors.
Recently the X-Ray intensity measurement system along with smart
X-Ray sensors is developed by our group which is able to detect
primary X-Ray intensity unerringly. In this study a new smart X-Ray
sensor is developed using Light-to-Frequency converter TSL230
from Texas Instruments which has numerous advantages in terms of
noiseless data acquisition and transmission. TSL230 construction is
based on a silicon photodiode which converts incoming X-Ray
radiation into the proportional current signal. A current to frequency
converter is attached to this photodiode on a single monolithic CMOS
integrated circuit which provides proportional frequency count to
incoming current signal in the form of the pulse train. The frequency
count is delivered to the center of PICDEM FS USB board with
PIC18F4550 microcontroller mounted on it. With highly compact
electronic hardware, this Demo Board efficiently read the smart
sensor output data. The frequency output approaches overcome
nonlinear behavior of sensors with analog output thus un-attenuated
X-Ray intensities could be measured precisely and better
normalization could be acquired in order to attain high resolution.
Abstract: The primary cause of Total Hip Replacement (THR)
failure for younger patients is aseptic loosening. This complication is
twice more likely to happen in acetabular cup than in femoral stem.
Excessive micromotion between bone and implant will cause
loosening and it depends in patient activities, age and bone. In this
project, the effects of different metal back design of press fit on
osseointegration of the acetabular cup are carried out. Commercial
acetabular cup designs, namely Spiked, Superfix and Quadrafix are
modelled and analyzed using commercial finite element software.
The diameter of acetabular cup is based on the diameter of acetabular
rim to make sure the component fit to the acetabular cavity. A new
design of acetabular cup are proposed and analyzed to get better
osseointegration between the bones and implant interface. Results
shows that the proposed acetabular cup designs are more stable
compared to other designs with respect to stress and displacement
aspects.
Abstract: The present research was designed to investigate the
anti-microbial activity of aristolochic acid from the root of
Aristolochia bracteata. From the methanolic & ethyl extract extracts
of Aristolochia bracteata aristolochic acid I was isolated and
conformed through IR, NMR & MS. The percentage purity of
aristolochic acid I was determined by UV & HPLC method. Antibacterial
activity of extracts of Aristolochia bracteata and the
isolated compound was determined by disc diffusion method. The
results reveled that the isolated aristolochic acid from methanolic
extract was more pure than the compound from ethyl acetate extract.
The various extracts (500μg/disc) of Aristolochia bracteata showed
moderate antibacterial activity with the average zone of inhibition of
7-18 mm by disc diffusion method. Among the extracts, ethyl acetate
& methanol extracts were shown good anti-microbial activity and the
growth of E.coli (18 mm) was strongly inhibited. Microbial assay of
isolated compound (Aristolochic acid I) from ethyl acetate &
methanol extracts were shown good antimicrobial activity and the
zone of inhibition of both at higher concentration 50 μg/ml was
similar with the standard aristolochic acid. It may be concluded that
the isolated compound of aristolochic acid I has good anti-bacterial
activity.
Abstract: Bones are dynamic and responsive organs, they
regulate their strength and mass according to the loads which they are subjected. Because, the Wnt/β-catenin pathway has profound
effects on the regulation of bone mass, we hypothesized that mechanical loading of bone cells stimulates Wnt/β-catenin signaling, which results in the generation of new bone mass.
Mechanical loading triggers the secretion of the Wnt molecule, which after binding to transmembrane proteins, causes GSK-3β (Glycogen synthase kinase 3 beta) to cease the phosphorylation of β-catenin. β-catenin accumulation in the cytoplasm, followed by its
transport into the nucleus, binding to transcription factors (TCF/LEF)
that initiate transcription of genes related to bone formation. To test this hypothesis, we used TOPGAL (Tcf Optimal Promoter
β-galactosidase) mice in an experiment in which cyclic loads were
applied to the forearm. TOPGAL mice are reporters for cells effected
by the Wnt/β-catenin signaling pathway. TOPGAL mice are genetically engineered mice in which transcriptional activation of β-
catenin, results in the production of an enzyme, β-galactosidase. The
presence of this enzyme allows us to localize transcriptional
activation of β-catenin to individual cells, thereby, allowing us to quantify the effects that mechanical loading has on the Wnt/β-catenin pathway and new bone formation. The ulnae of loaded TOPGAL
mice were excised and transverse slices along different parts of the
ulnar shaft were assayed for the presence of β-galactosidase.
Our results indicate that loading increases β-catenin transcriptional
activity in regions where this pathway is already primed (i.e. where basal activity is already higher) in a load magnitude dependent
manner. Further experiments are needed to determine the temporal and spatial activation of this signaling in relation to bone formation.
Abstract: To compare Healing Effects of an
Ayurvedic Preparation and Silver Sulfadiazine on burn wounds in
Albino Rats.
Methods: Albino rats– 30 male / female rats weighing between
150-200 g were used in the study. They were individually housed and
maintained on normal diet and water ad libitum. Partial thickness
burn wounds were inflicted, on overnight-starved animals under
pentobarbitone (30mg/kg, i.p.) anaesthesia, by pouring hot molten
wax at 80oC into a plastic cylinder of 300 mm2 circular openings
placed on the shaven back of the animal. Apart from the drugs under
investigation no local/ systemic chemotherapeutic cover will be
provided to animals. All the animals were assessed for the percentage
of wound contraction, signs of infection, scab formation and
histopathological examination.
Results: Percentage of wound healing was significantly better in
the test ointment group compared to the standard. Signs of infection
were observed in more animals in the test ointment group compared
to the standard. Scab formation also took place earlier in the test
ointment group compared to standard. Epithelial regeneration and
healing profile was better in the test ointment compared to the
standard. Moreover the test ointment group did not show any raised
margins in the wound or blackish discoloration as was observed in
silver sulfadiazine group.
Conclusion: The burn wound healing effect of the ayurvedic
ointment under study is better in comparison to standard therapy of
silver sulfadiazine. The problem of infection encountered with the
test ointment can be overcome by changing the concentrations and
proportions of the ingredients in the test ointment which constitutes
the further plan of the study.
Abstract: In Multiple Sclerosis, pathological changes in the
brain results in deviations in signal intensity on Magnetic Resonance
Images (MRI). Quantitative analysis of these changes and their
correlation with clinical finding provides important information for
diagnosis. This constitutes the objective of our work. A new approach
is developed. After the enhancement of images contrast and the brain
extraction by mathematical morphology algorithm, we proceed to the
brain segmentation. Our approach is based on building statistical
model from data itself, for normal brain MRI and including clustering
tissue type. Then we detect signal abnormalities (MS lesions) as a
rejection class containing voxels that are not explained by the built
model. We validate the method on MR images of Multiple Sclerosis
patients by comparing its results with those of human expert
segmentation.