Abstract: Researchers of drug-drug interaction alert systems
have often suggested that there were high overridden rate for alerts and
also too false alerts. However, research about decreasing false alerts is
scant. Therefore, the aim of this article attempts to proactive
identification of false alert for drug-drug interaction and provide
solution to decrease false alerts. This research involved retrospective
analysis prescribing database and calculated false alert rate by using
MYSQL and JAVA. Results of this study showed 17% of false alerts
and the false alert rate in the hospitals (37%) was more than in the
clinics. To conclude, this study described the importance that
drug-drug interaction alert system should not only detect drug name
but also detect frequency or route, as well as in providing solution to
decrease false alerts.
Abstract: Phase-Contrast MR imaging methods are widely used
for measurement of blood flow velocity components. Also there are
some other tools such as CT and Ultrasound for velocity map
detection in intravascular studies. These data are used in deriving
flow characteristics. Some clinical applications are investigated
which use pressure distribution in diagnosis of intravascular disorders
such as vascular stenosis. In this paper an approach to the problem of
measurement of intravascular pressure field by using velocity field
obtained from flow images is proposed. The method presented in this
paper uses an algorithm to calculate nonlinear equations of Navier-
Stokes, assuming blood as an incompressible and Newtonian fluid.
Flow images usually suffer the lack of spatial resolution. Our
attempt is to consider the effect of spatial resolution on the pressure
distribution estimated from this method. In order to achieve this aim,
velocity map of a numerical phantom is derived at six different
spatial resolutions. To determine the effects of vascular stenoses on
pressure distribution, a stenotic phantom geometry is considered. A
comparison between the pressure distribution obtained from the
phantom and the pressure resulted from the algorithm is presented. In
this regard we also compared the effects of collocated and staggered
computational grids on the pressure distribution resulted from this
algorithm.
Abstract: Quantitative measurements of tumor in general and tumor volume in particular, become more realistic with the use of Magnetic Resonance imaging, especially when the tumor morphological changes become irregular and difficult to assess by clinical examination. However, tumor volume estimation strongly depends on the image segmentation, which is fuzzy by nature. In this paper a fuzzy approach is presented for tumor volume segmentation based on the fuzzy connectedness algorithm. The fuzzy affinity matrix resulting from segmentation is then used to estimate a fuzzy volume based on a certainty parameter, an Alpha Cut, defined by the user. The proposed method was shown to highly affect treatment decisions. A statistical analysis was performed in this study to validate the results based on a manual method for volume estimation and the importance of using the Alpha Cut is further explained.
Abstract: An alarming emergence of multidrug-resistant strains
of the tuberculosis pathogen Mycobacterium tuberculosis and
continuing high worldwide incidence of tuberculosis has invigorated
the search for novel drug targets. The enzyme glutamate racemase
(MurI) in bacteria catalyzes the stereoconversion of L-glutamate to
D-glutamate which is a component of the peptidoglycan cell wall of
the bacterium. The inhibitors targeted against MurI from several
bacterial species have been patented and are advocated as promising
antibacterial agents. However there are none available against MurI
from Mycobacterium tuberculosis, due to the lack of its threedimensional
structure. This work accomplished two major objectives.
First, the tertiary structure of MtMurI was deduced computationally
through homology modeling using the templates from bacterial
homologues. It is speculated that like in other Gram-positive bacteria,
MtMurI exists as a dimer and many of the protein interactions at the
dimer interface are also conserved. Second, potent candidate
inhibitors against MtMurI were identified through docking against
already known inhibitors in other organisms.
Abstract: In this study, a new and fast algorithm for Ascending
Aorta (AscA) and Descending Aorta (DesA) segmentation is
presented using Computed Tomography Angiography images. This
process is quite important especially at the detection of aortic
plaques, aneurysms, calcification or stenosis. The applied method has
been carried out at four steps. At first step, lung segmentation is
achieved. At the second one, Mediastinum Region (MR) is detected
to use in the segmentation. At the third one, images have been
applied optimal threshold and components which are outside of the
MR were removed. Lastly, identifying and segmentation of AscA and
DesA have been carried out. The performance of the applied method
is found quite well for radiologists and it gives enough results to the
surgeries medically.
Abstract: In this study, the powders of Ni and Ti with 50.5 at.%
Ni for 12 h were blended and cold pressed at the different pressures
(50, 75 and100 MPa).The porous product obtained after Ni-Ti
compacts were synthesized by SHS (self-propagating hightemperature
synthesis) in the different preheating temperatures (200,
250 and 300oC) and heating rates (30, 60 and 90oC/min). The effects
of the pressure, preheating temperature and heating rate were
investigated on biocompatibility in vivo. The porosity in the
synthesized products was in the range of 50.7–59.7 vol. %. The
pressure, preheating temperature and heating rate were found to have
an important effect on the biocompatibility in-vivo of the synthesized
products. Max. fibrotic tissue within the porous implant was found in
vivo periods (6 months), in which compacting pressure 100MPa.
Abstract: Cancer classification to their corresponding cohorts has been key area of research in bioinformatics aiming better prognosis of the disease. High dimensionality of gene data has been makes it a complex task and requires significance data identification technique in order to reducing the dimensionality and identification of significant information. In this paper, we have proposed a novel approach for classification of oral cancer into metastasis positive and negative patients. We have used significance analysis of microarrays (SAM) for identifying significant genes which constitutes gene signature. 3 different gene signatures were identified using SAM from 3 different combination of training datasets and their classification accuracy was calculated on corresponding testing datasets using k-Nearest Neighbour (kNN), Fuzzy C-Means Clustering (FCM), Support Vector Machine (SVM) and Backpropagation Neural Network (BPNN). A final gene signature of only 9 genes was obtained from above 3 individual gene signatures. 9 gene signature-s classification capability was compared using same classifiers on same testing datasets. Results obtained from experimentation shows that 9 gene signature classified all samples in testing dataset accurately while individual genes could not classify all accurately.
Abstract: Sensorized instruments that accurately measure the interaction forces (between biological tissue and instrument endeffector) during surgical procedures offer surgeons a greater sense of immersion during minimally invasive robotic surgery. Although there is ongoing research into force measurement involving surgical graspers little corresponding effort has been carried out on the measurement of forces between scissor blades and tissue. This paper presents the design and development of a force measurement test apparatus, which will serve as a sensor characterization and evaluation platform. The primary aim of the experiments is to ascertain whether the system can differentiate between tissue samples with differing mechanical properties in a reliable, repeatable manner. Force-angular displacement curves highlight trends in the cutting process as well the forces generated along the blade during a cutting procedure. Future applications of the test equipment will involve the assessment of new direct force sensing technologies for telerobotic surgery.
Abstract: In this study, we explore the use of information for inventory decision in the healthcare organization (HO). We consider the scenario when the HO can make use of the information collected from some correlated products to enhance its inventory planning. Motivated by our real world observations that HOs adopt RFID and bar-coding system for information collection purpose, we examine the effectiveness of these systems for inventory planning with Bayesian information updating. We derive the optimal ordering decision and study the issue of Pareto improvement in the supply chain. Our analysis demonstrates that RFID system will outperform the bar-coding system when the RFID system installation cost and the tag cost reduce to a level that is comparable with that of the barcoding system. We also show how an appropriately set wholesale pricing contract can achieve Pareto improvement in the HO supply chain.
Abstract: The scenario of bypass transition is generally described
as follows: the low-frequency disturbances in the free-stream may
generate long stream-wise streaks in the boundary layer, which later
may trigger secondary instability, leading to rapid increase of
high-frequency disturbances. Then possibly turbulent spots emerge,
and through their merging, lead to fully developed turbulence. This
description, however, is insufficient in the sense that it does not
provide the inherent mechanism of transition that during the transition,
a large number of waves with different frequencies and wave numbers
appear almost simultaneously, producing sufficiently large Reynolds
stress, so the mean flow profile can change rapidly from laminar to
turbulent. In this paper, such a mechanism will be figured out from
analyzing DNS data of transition.
Abstract: The article deals with technical support of intracranial single unit activity measurement. The parameters of the whole measuring set were tested in order to assure the optimal conditions of extracellular single-unit recording. Metal microelectrodes for measuring the single-unit were tested during animal experiments. From signals recorded during these experiments, requirements for the measuring set parameters were defined. The impedance parameters of the metal microelectrodes were measured. The frequency-gain and autonomous noise properties of preamplifier and amplifier were verified. The measurement and the description of the extracellular single unit activity could help in prognoses of brain tissue damage recovery.
Abstract: Liposomal magnetofection is the most powerful nonviral method for the nucleic acid delivery into the cultured cancer cells and widely used for in vitro applications. Use of the static magnetic field condition may result in non-uniform distribution of aggregate complexes on the surface of cultured cells. To prevent this, we developed the new device which allows to concentrate aggregate complexes under dynamic magnetic field, assisting more contact of these complexes with cellular membrane and, possibly, stimulating endocytosis. Newly developed device for magnetofection under dynamic gradient magnetic field, “DynaFECTOR", was used to compare transfection efficiency of human liver hepatocellular carcinoma cell line HepG2 with that obtained by lipofection and magnetofection. The effect of two parameters on transfection efficiency, incubation time under dynamic magnetic field and rotation frequency of magnet, was estimated. Liposomal magnetofection under dynamic gradient magnetic field showed the highest transfection efficiency for HepG2 cells.
Abstract: Availability of high dimensional biological datasets such as from gene expression, proteomic, and metabolic experiments can be leveraged for the diagnosis and prognosis of diseases. Many classification methods in this area have been studied to predict disease states and separate between predefined classes such as patients with a special disease versus healthy controls. However, most of the existing research only focuses on a specific dataset. There is a lack of generic comparison between classifiers, which might provide a guideline for biologists or bioinformaticians to select the proper algorithm for new datasets. In this study, we compare the performance of popular classifiers, which are Support Vector Machine (SVM), Logistic Regression, k-Nearest Neighbor (k-NN), Naive Bayes, Decision Tree, and Random Forest based on mock datasets. We mimic common biological scenarios simulating various proportions of real discriminating biomarkers and different effect sizes thereof. The result shows that SVM performs quite stable and reaches a higher AUC compared to other methods. This may be explained due to the ability of SVM to minimize the probability of error. Moreover, Decision Tree with its good applicability for diagnosis and prognosis shows good performance in our experimental setup. Logistic Regression and Random Forest, however, strongly depend on the ratio of discriminators and perform better when having a higher number of discriminators.
Abstract: One of the essential requirements in order to have a
realistic surgical simulator is real-time interaction by means of a
haptic interface is. In fact, reproducing haptic sensations increases
the realism of the simulation. However, the interaction need to be
performed in real-time, since a delay between the user action and the
system reaction reduces the user immersion. In this paper, we present
a prototype of the coronary stent implant simulator developed in the
HERMES Project; this system allows real-time interactions with a
artery by means of a specific haptic device; thus the user can
interactively navigate in a reconstructed artery and force feedback is
produced when contact occurs between the artery walls and the
medical instruments
Abstract: Prostate cancer is one of the most frequent cancers in men and is a major cause of mortality in the most of countries. In many diagnostic and treatment procedures for prostate disease accurate detection of prostate boundaries in transrectal ultrasound (TRUS) images is required. This is a challenging and difficult task due to weak prostate boundaries, speckle noise and the short range of gray levels. In this paper a novel method for automatic prostate segmentation in TRUS images is presented. This method involves preprocessing (edge preserving noise reduction and smoothing) and prostate segmentation. The speckle reduction has been achieved by using stick filter and top-hat transform has been implemented for smoothing. A feed forward neural network and local binary pattern together have been use to find a point inside prostate object. Finally the boundary of prostate is extracted by the inside point and an active contour algorithm. A numbers of experiments are conducted to validate this method and results showed that this new algorithm extracted the prostate boundary with MSE less than 4.6% relative to boundary provided manually by physicians.
Abstract: The counting and analysis of blood cells allows the
evaluation and diagnosis of a vast number of diseases. In particular,
the analysis of white blood cells (WBCs) is a topic of great interest to
hematologists. Nowadays the morphological analysis of blood cells is
performed manually by skilled operators. This involves numerous
drawbacks, such as slowness of the analysis and a nonstandard
accuracy, dependent on the operator skills. In literature there are only
few examples of automated systems in order to analyze the white
blood cells, most of which only partial. This paper presents a
complete and fully automatic method for white blood cells
identification from microscopic images. The proposed method firstly
individuates white blood cells from which, subsequently, nucleus and
cytoplasm are extracted. The whole work has been developed using
MATLAB environment, in particular the Image Processing Toolbox.
Abstract: Today, cancer remains one of the major diseases that
lead to death. The main obstacle in chemotherapy as a main cancer
treatment is the toxicity to normal cells due to Multidrug Resistance
(MDR) after the use of anticancer drugs. Proposed solution to
overcome this problem is the use of MDR efflux inhibitor of cinchona
alkaloids which is delivered together with anticancer drugs
encapsulated in the form of polymeric nanoparticles. The particles
were prepared by the hydration method. The characterization of
nanoparticles was particle size, zeta potential, entrapment efficiency
and in vitro drug release. Combination nanoparticle size ranged 29-45
nm with a neutral surface charge. Entrapment efficiency was above
87% for the use quinine, quinidine or cinchonidine in combination
with etoposide. The release test results exhibited that the cinchona
alkaloids release released faster than that of etoposide. Collectively,
cinchona alkaloids can be packaged along with etoposide in
nanomicelles for better cancer therapy.
Abstract: This paper explores the opportunity of using tri-axial
wireless accelerometers for supervised monitoring of sports
movements. A motion analysis system for the upper extremities of
lawn bowlers in particular is developed. Accelerometers are placed
on parts of human body such as the chest to represent the shoulder
movements, the back to capture the trunk motion, back of the hand,
the wrist and one above the elbow, to capture arm movements. These
sensors placement are carefully designed in order to avoid restricting
bowler-s movements. Data is acquired from these sensors in soft-real
time using virtual instrumentation; the acquired data is then
conditioned and converted into required parameters for motion
regeneration. A user interface was also created to facilitate in the
acquisition of data, and broadcasting of commands to the wireless
accelerometers. All motion regeneration in this paper deals with the
motion of the human body segment in the X and Y direction, looking
into the motion of the anterior/ posterior and lateral directions
respectively.
Abstract: A five-class density histogram with an index named cumulative density was proposed to analyze the short-term HRV. 150 subjects participated in the test, falling into three groups with equal numbers -- the healthy young group (Young), the healthy old group (Old), and the group of patients with congestive heart failure (CHF). Results of multiple comparisons showed a significant differences of the cumulative density in the three groups, with values 0.0238 for Young, 0.0406 for Old and 0.0732 for CHF (p
Abstract: This paper aims at presenting the biotechnology used
to obtain collagen-based gels from shark (Squalus acanthias) and brill
skin, marine fish growing in the Black Sea. Due to the structure of its
micro-fibres, collagen can be considered a nanomaterial; in order to
use collagen-based matrixes as biomaterial, rheological studies must
be performed first, to state whether they are stable or not. For the
triple-helix structure to remain stable within these gels at room or
human body temperature, they must be stabilized by reticulation.