Abstract: In production of medicinal plants, seed germination is
very important problem. The treated seeds (control, hydro priming
and ZnSO4) of Cumin (Cuminum cyminum L.) were evaluated at
germination and seedling growth for tolerance to salt (NaCl and
Na2SO4) conditions at the same water potentials of 0.0, -0.3, -0.6, -
0.9 and -1.2MPa. Electrical conductivity (EC) values of the NaCl
solutions were 0.0, 6.5, 12.7, 18.4 and 23.5 dSm-1, respectively. The
objective of the study was to determine factors responsible for
germination and early seedling growth due to salt toxicity or osmotic
effect and to optimize the best priming treatment for these stress
conditions. Results revealed that germination delayed in both
solutions, having variable germination with different priming
treatments. Germination, shoot and weight, root and shoot length
were higher but mean germination time and abnormal germination
percentage were lower in NaCl than Na2SO4 at the same water
potential. The root / shoot weight and R/S length increased with
increase in osmotic potential in both NaCl and Na2SO4 solutions.
NaCl had less inhibitor effect on seedling growth than the
germination. It was concluded that inhibition of germination at the
same water potential of NaCl and Na2SO4 resulted from salt toxicity
rather than osmotic effect. Hydro priming increased germination and
seedling growth under salt stress. This protocol has practical
importance and could be recommended to farmers to achieve higher
germination and uniform emergence under field conditions.
Abstract: Pentachlorophenol (PCP) is a polychlorinated
aromatic compound that is widespread in industrial effluents and is
considered to be a serious pollutant. Among the variety of industrial
effluents encountered, effluents from tanning industry are very
important and have a serious pollution potential. PCP is also formed
unintentionally in effluents of paper and pulp industries. It is highly
persistent in soils and is lethal to a wide variety of beneficial
microorganisms and insects, human beings and animals. The natural
processes that breakdown toxic chemicals in the environment have
become the focus of much attention to develop safe and environmentfriendly
deactivation technologies. Microbes and plants are among
the most important biological agents that remove and degrade waste
materials to enable their recycling in the environment. The present
investigation was carried out with the aim of developing a microbial
system for bioremediation of PCP polluted soils. A number of plant
species were evaluated for their ability to tolerate different
concentrations of pentachlorophenol (PCP) in the soil. The
experiment was conducted for 30 days under pot culture conditions.
The toxic effect of PCP on plants was studied by monitoring seed
germination, plant growth and biomass. As the concentration of PCP
was increased to 50 ppm, the inhibition of seed germination, plant
growth and biomass was also increased. Although PCP had a
negative effect on all plant species tested, maize and groundnut
showed the maximum tolerance to PCP. Other tolerating crops
included wheat, safflower, sunflower, and soybean. From the
rhizosphere soil of the tolerant seedlings, as many as twenty seven
PCP tolerant bacteria were isolated. From soybean, 8; sunflower, 3;
safflower 8; maize 2; groundnut and wheat, 3 each isolates were
made. They were screened for their PCP degradation potentials.
HPLC analyses of PCP degradation revealed that the isolate MAZ-2
degraded PCP completely. The isolate MAZ-1 was the next best
isolate with 90 per cent PCP degradation. These strains hold promise
to be used in the bioremediation of PCP polluted soils.
Abstract: The PRAF family of proteins is a plant specific family of proteins with distinct domain architecture and various unique sequence/structure traits. We have carried out an extensive search of the Arabidopsis genome using an automated pipeline and manual methods to verify previously known and identify unknown instances of PRAF proteins, characterize their sequence and build 3D structures of their individual domains. Integrating the sequence, structure and whatever little known experimental details for each of these proteins and their domains, we present a comprehensive characterization of the different domains in these proteins and their variant properties.
Abstract: The paper presents the influence of the conventional
ploughing tillage technology in comparison with the minimum
tillage, upon the soil properties, weed control and yield in the case of
maize (Zea mays L.), soya-bean (Glycine hispida L.) and winter
wheat (Triticum aestivum L.) in a three years crop rotation. A
research has been conducted at the University of Agricultural
Sciences and Veterinary Medicine Cluj-Napoca, Romania. The use of
minimum soil tillage systems within a three years rotation: maize,
soya-bean, wheat favorites the rise of the aggregates hydro stability
with 5.6-7.5% on a 0-20 cm depth and 5-11% on 20-30 cm depth.
The minimum soil tillage systems – paraplow, chisel or rotary grape
– are polyvalent alternatives for basic preparation, germination bed
preparation and sowing, for fields and crops with moderate loose
requirements being optimized technologies for: soil natural fertility
activation and rationalization, reduction of erosion, increasing the
accumulation capacity for water and realization of sowing in the
optimal period. The soil tillage system influences the productivity
elements of cultivated species and finally the productions thus
obtained. Thus, related to conventional working system, the
productions registered in minimum tillage working represented 89-
97% in maize, 103-112% in soya-bean, 93-99% in winter-wheat. The
results of investigations showed that the yield is a conclusion soil
tillage systems influence on soil properties, plant density assurance
and on weed control. Under minimum tillage systems in the case of
winter weat as an option for replacing classic ploughing, the best
results in terms of quality indices were obtained from version worked
with paraplow, followed by rotary harrow and chisel. At variants
worked with paraplow were obtained quality indices close to those of
the variant worked with plow, and protein and gluten content was
even higher. At Ariesan variety, highest protein content, 12.50% and
gluten, 28.6% was obtained for the variant paraplow.
Abstract: This study was conducted to evaluate the antifungal
activities of Cinnamomum zeylanicum and Origanum vulgare L.
essential oil against Aspergillus flavus in culture media and tomato
paste. 200 ppm of cinnamon and 500 ppm of oregano completely
inhibited A. flavus growth in culture media, while in tomato paste 300
ppm of cinnamon and 200 ppm of oregano had the same effect. Test
panel evaluations revealed that samples with 100 and 200 ppm
cinnamon were acceptable. The results may suggest the potential use
of Cinnamomum zeylanicum essential oil as natural preservative in
tomato paste.
Abstract: Competitive relationships among Bradyrhizobium
japonicum USDA serogroup 123, 122 and 138 were screened versus
the standard commercial soybean variety Williams and two
introductions P1 377578 "671" in a field trial. Displacement of strain
123 by an effective strain should improved N2 fixation. Root nodules
were collected and strain occupancy percentage was determined
using strain specific fluorescent antibodies technique. As anticipated
the strain USDA 123 dominated 92% of nodules due to the high
affinity between the host and the symbiont. This dominance was
consistent and not changed materially either by inoculation practice
or by introducing new strainan. The interrelationship between the
genotype Williams and serogroup 122 & 138 was found very weak
although the cell density of the strain in the rhizosphere area was
equal. On the other hand, the nodule occupancy of genotypes 671 and
166 with rhizobia serogroup 123 was almost diminished to zero. .
The data further exhibited that the genotypes P1 671 and P1 166 have
high affinity to colonize with strains 122 and 138 whereas Williams
was highly promiscuous to strain 123.
Abstract: This study aims to screen out and to optimize the
major nutrients for maximum carotenoid production and
antioxidation characteristics by Rhodotorula rubra. It was found that
supplementary of 10 g/l glucose as carbon source, 1 g/l ammonium
sulfate as nitrogen source and 1 g/l yeast extract as growth factor in
the medium provided the better yield of carotenoid content of 30.39
μg/g cell dry weight the amount of antioxidation of Rhodotorula
rubra by DPPH, ABTS and MDA method were 1.463%, 34.21% and
34.09 μmol/l, respectively.
Abstract: The complex structure of lignocellulose leads to great
difficulties in converting it to fermentable sugars for the ethanol
production. The major hydrolysis impediments are the crystallinity of
cellulose and the lignin content. To improve the efficiency of
enzymatic hydrolysis, microbial pretreatment of corncob was
investigated using two bacterial strains of Bacillus subtilis A 002 and
Cellulomonas sp. TISTR 784 (expected to break open the crystalline
part of cellulose) and lignin-degrading fungus, Phanerochaete
sordida SK7 (expected to remove lignin from lignocellulose). The
microbial pretreatment was carried out with each strain under its
optimum conditions. The pretreated corncob samples were further
hydrolyzed to produce reducing glucose with low amounts of
commercial cellulase (25 U·g-1 corncob) from Aspergillus niger. The
corncob samples were determined for composition change by X-ray
diffraction (XRD), Fourier transform infrared spectroscopy (FTIR),
and scanning electron microscope (SEM). According to the results,
the microbial pretreatment with fungus, P. sordida SK7 was the most
effective for enhancing enzymatic hydrolysis, approximately, 40%
improvement.
Abstract: A three-year field experiment (2010-2012) was
conducted to determine the abundance of epiphytic and endophytic
filamentous fungi colonizing the grain of winter wheat cv. Bogatka.
Wheat spikes were protected with tebuconazole or chitosan at the
watery ripe stage. Untreated plants served as control. Tebuconazole
exerted an inhibitory effect primarily on F. culmorum and F.
graminearum, and its effectiveness was determined by the pressure
from pathogens that infected wheat spikes during the growing season.
Chitosan did not suppress the growth of Fusarium species and
Alternaria alternata.
Abstract: Wheat prediction was carried out using different meteorological variables together with agro meteorological indices in Ardebil district for the years 2004-2005 & 2005–2006. On the basis of correlation coefficients, standard error of estimate as well as relative deviation of predicted yield from actual yield using different statistical models, the best subset of agro meteorological indices were selected including daily minimum temperature (Tmin), accumulated difference of maximum & minimum temperatures (TD), growing degree days (GDD), accumulated water vapor pressure deficit (VPD), sunshine hours (SH) & potential evapotranspiration (PET). Yield prediction was done two months in advance before harvesting time which was coincide with commencement of reproductive stage of wheat (5th of June). It revealed that in the final statistical models, 83% of wheat yield variability was accounted for variation in above agro meteorological indices.
Abstract: Coriander is an annual and herbaceous plant, belong to
the apiaceae family. This plant is cultivated world widely. It is well
known for having medicinal properties. The aim of this experiment
was to study seed quality of species grown in Kermanshah
conditions. The experiment was carried out in research farm, Campus
of Agriculture and Natural Resources, Razi University, Kermanshah,
Iran. Coriander (local type) was grown in late spring May (5th and
20th) and Jun (4th and 19th), and plant density (10, 30, 50 and 70
plants m-2) in 2009. The experimental plots were laid out in a
factorial according to a randomized complete block design with three
replications. The fruits were harvest between 83.5 – 106.5 days after
sowing. The essential oil and oil content was extracted by Clevenger
and Soxhlet apparatuses, respectively. Results showed that delay at
planting date increased the oil content. Also, with the increase at
plant density was decreased oil content and essential oil.
Abstract: Various assisted reproductive techniques have been
developed and refined to obtain a large number of offspring from
genetically superior animals or obtain offspring from infertile (or
subfertile) animals. The embryo transfer is one assisted reproductive
technique developed well, aimed at increased productivity of selected
females, disease control, importation and exportation of livestock,
rapid screening of AI sires for genetically recessive characteristics,
treatment or circumvention of certain types of infertility. Embryo
transfer also is a useful research tool for evaluating fetal and maternal
interactions. This technique has been applied to nearly every species
of domestic animal and many species of wildlife and exotic animals,
including humans and non-human primates. The successful of
embryo transfers have been limited to within-animal, homologous
replacement of the embryos. There are several examples of
interspecific and intergeneric embryo transfers in which embryos
implanted but did not develop to term: sheep and goat, mouse and rat.
An immunological rejections and placental incompatibility between
the embryo and the surrogate mother appear to restrict interspecific
embryo transfer/interspecific pregnancy. Recently, preimplantation
embryo manipulation procedures have been applied, such as
technique of inner cell mass transfer. This technique will possible to
overcome the reproductive barrier interspecific embryo
transfer/interspecific pregnancy, if there is a protective mechanism
which prevents recognition of the foreign fetus by the mother of the
other species
Abstract: In most wheat growing moderate regions and
especially in the north of Iran climate, is affected grain filling by
several physical and abiotic stresses. In this region, grain filling often
occurs when temperatures are increasing and moisture supply is
decreasing. The experiment was designed in RCBD with split plot
arrangements with four replications. Four irrigation treatments
included (I0) no irrigation (check); (I1) one irrigation (50 mm) at
heading stage; (I2) two irrigation (100 mm) at heading and anthesis
stage; and (I3) three irrigation (150 mm) at heading, anthesis and
early grain filling growth stage, two wheat cultivars (Milan and
Shanghai) were cultured in the experiment. Totally raining was 453
mm during the growth season. The result indicated that biological
yield, grain yield and harvest index were significantly affected by
irrigation levels. I3 treatment produced more tillers number in m2,
fertile tillers number in m2, harvest index and biological yield. Milan
produced more tillers number in m2, fertile tillers in m2, while
Shanghai produced heavier tillers and grain 1000 weight. Plant height
was significant in wheat varieties while were not statistically
significant in irrigation levels. Milan produced more grain yield,
harvest index and biological yield. Grain yield shown that I1, I2, and
I3 produced increasing of 5228 (21%), 5460 (27%) and 5670 (29%)
kg ha-1, respectively. There was an interaction of irrigation and
cultivar on grain yields. In the absence of the irrigation reduced grain
1000 weight from 45 to 40 g. No irrigation reduced soil moisture
extraction during the grain filling stage. Current assimilation as a
source of carbon for grain filling depends on the light intercepting
viable green surfaces of the plant after anthesis that due to natural
senescence and the effect of various stresses. At the same time the
demand by the growing grain is increasing. It is concluded from
research work that wheat crop irrigated Milan cultivar could increase
the grain yield in comparison with Shanghai cultivar. Although, the
grain yield of Shanghai under irrigation was slightly lower than
Milan. This grain yield also was related to weather condition, sowing
date, plant density and location conditions and management of
fertilizers, because there was not significant difference in biological
and straw yield. The best result was produced by I1 treatment. I2 and
I3 treatments were not significantly difference with I1 treatment.
Grain yield of I1 indicated that wheat is under soil moisture
deficiency. Therefore, I1 irrigation was better than I0.
Abstract: Sweet potato products are necessary for the provision
of essential nutrients in every household, regardless of their poverty
status. Their consumption appears to be highly influenced by socioeconomic
factors, such as malnutrition, food insecurity and
unemployment. Therefore, market availability is crucial for these
cultivars to resolve some of the socio-economic factors. The aim of
the study was to investigate market availability of sweet potato
cultivars in the North West Province. In this study, both qualitative
and quantitative research methodologies were used. Qualitative
methodology was used to explain the quantitative outcomes of the
variables. On the other hand, quantitative results were used to test the
hypothesis. The study used SPSS software to analyse the data. Crosstabulation
and Chi-square statistics were used to obtain the
descriptive and inferential analyses, respectively. The study found
that the Blesbok cultivar is dominating the markets of the North West
Province, with the Monate cultivar dominating in the Bojanala
Platinum (75%) and Dr Ruth Segomotsi Mompati (25%) districts. It
is also found that a unit increase in the supply of sweet potato
cultivars in both local and district municipal markets is accompanied
by a reduced demand of 28% and 33% at district and local markets,
respectively. All these results were found to be significant at p
Abstract: Processes of plant breeding, testing and licensing of new varieties, patent protection in seed production, relations in trade and protection of copyright are dependent on identification, differentiation and characterization of plant genotypes. Therefore, we focused our research on utilization of wheat storage proteins as genetic markers suitable not only for differentiation of individual genotypes, but also for identification and characterization of their considerable properties. We analyzed a collection of 102 genotypes of bread wheat (Triticum aestivum L.), 41 genotypes of spelt wheat (Triticum spelta L.), and 35 genotypes of durum wheat (Triticum durum Desf.), in this study. Our results show, that genotypes of bread wheat and durum wheat were homogenous and single line, but spelt wheat genotypes were heterogenous. We observed variability of HMW-GS composition according to environmental factors and level of breeding and predict technological quality on the basis of Glu-score calculation.
Abstract: This study aims at using multi-source data to monitor
coral biodiversity and coral bleaching. We used coral reef at Racha
Islands, Phuket as a study area. There were three sources of data:
coral diversity, sensor based data and satellite data.
Abstract: Protein and Esterase electrophoresis were used to
genetically identify two Saudi tick species. Engorged females of the
camel tick Hyalomma dromedarii (Koch) (Acari: Ixodidae) and the
cattle tick Boophilus annulatus (Say) (Acari: Ixodidae) ticks
collected from infested camels and cattle in the animals resting
house at Hail region in KSA were used. The results showed that
there are a variation in both of protein and esterase activity levels and
a high polymorphism within and between the genera and species of
Hyalomma and Boophilus . In conclusion, the protein and esterase
electrophoretic analysis used in the present study could successfully
distinguish among tick species, commonly found in Saudi Arabia.
Abstract: Oxyleotris marmorata is considered as
undomesticated fish, and its culture occasionally faces a problem of
food deprivation. The present study aims to evaluate alteration of
hematological indices, blood chemical associated with liver function
during 4 weeks of fasting. A non-linear relationships between fasting
days and hematological parameters (red blood cell number; y = -
0.002x2 + 0.041x + 1.249; R2=0.915, P0.05), mean corpuscular
volume; y = -0.180x2 + 2.183x + 149.61; R2=0.732, P>0.05, mean
corpuscular hemoglobin; y = -0.041x2 + 0.862x + 29.864; R2=0.818,
P>0.05 and mean corpuscular hemoglobin concentration; y = -
0.044x2 + 0.711x + 21.580; R2=0.730, P>0.05) were demonstrated.
Significant change in hematocrit (Ht) during fasting period was
observed. Ht elevated sharply increase at the first weeks of fasting
period. Higher Ht also was detected during week 2-4 of fasting time.
The significant reduction of hepatosomatic index was observed (y = -
0.007x2 - 0.096x + 1.414; R2=0.968, P0.05, serum glutamic oxaloacetic transaminase;
y = 0.005x2 – 0.201x2 + 1.297x + 33.256; R2=1, P0.05). Taken together, prolonged fasting has
deleterious effects on hematological indices, liver mass and enzyme
associated in liver function. The marked adverse effects occurred
after the first week of fasting state.
Abstract: The cDNA encoding the 326 amino acids of a Class I
basic chitinase gene from Leucaena leucocephala de Wit (KB3,
Genbank accession: AAM49597) was cloned under the control of
CaMV35S promoter in pCAMBIA 1300 and transferred to
Koshihikari. Calli of Koshihikari rice was transformed with
agrobacterium with this construct expressing the chitinase and β-
glucouronidase (GUS). The frequencies of calli 90 % has been
obtained from rice seedlings cultured on NB medium. The high
regeneration frequencies, 74% was obtained from calli cultured on
regeneration medium containing 4 mg/l BAP, and 7 g/l phytagel at
25°C. Various factors were studied in order to establish a procedure
for the transformation of Koshihikari Agrobacterium tumefaciens.
Supplementation of 50 mM acetosyringone to the medium during
coculivation was important to enhance the frequency to transient
transformation. The 4 week-old scutellum-derived calli were
excellent starting materials. Selection medium based on NB medium
supplement with 40 mg/l hygromycin and 400 mg/l cefotaxime were
an optimized medium for selection of transformed rice calli. The
percentage of transformation 70 was obtained. Recombinant calli and
regenerated rice plants were checked the expression of chitinase and
gus by PCR, northern blot gel, southern blot gel, and gus assay.
Chitinase and gus were expressed in all parts of recombinant rice.
The rice line expressing the KB3 chiitnase was more resistant to the
blast fungus Fusarium monoliforme than control line.
Abstract: The avian phytohaemagglutinin skin test is being
proved as an in vivo system for the evaluation an avian in vivo T cell
mitogenicity. The test system was one week old Gallus domesticus
broiler Chickens. Five replicates were done for each of the whole,
1:10 dilutions of each of 0.05 IU tuberculin, tetanus immunoglobulin
and DPT vaccine as test materials. The evaluation parameters were
the skin indurations and lymphoblast percentages in bone marrow
lymphocytes.
Tuberculin indurations were 2.06 and 1.26mm for 0.05 IU
respectively while lymphoblast percent were 0.234 and 0.1
accordingly.
The skin indurations of 135mg/ml and 1.35mg/ml tetanus
immunoglobulin were 4.86 and 3.96mm while lymphoblast
percentages were 0.3 and 0.14 respectively.
The whole DPT and 1:10 concentration were with 4.5 and 3.2mm
while their lymphoblast percentages were 0.28 and 0.12 accordingly.
Thus the mitogenicity of the test materials was of dependant type.