Abstract: Wheat gluten hydrolyzates (WGHs) and anchovy fine
powder hydrolyzates (AFPHs) were produced at 300 MPa using
combinations of Flavourzyme 500MG (F), Alcalase 2.4L (A),
Marugoto E (M) and Protamex (P), and then were compared to those
produced at ambient pressure concerning the contents of soluble solid
(SS), soluble nitrogen and electrophoretic profiles. The contents of SS
in the WGHs and AFPHs increased up to 87.2% according to the
increase in enzyme number both at high and ambient pressure. Based
on SS content, the optimum enzyme combinations for one-, two-,
three- and four-enzyme hydrolysis were determined as F, FA, FAM
and FAMP, respectively. Similar trends were found for the contents of
total soluble nitrogen (TSN) and TCA-soluble nitrogen (TCASN). The
contents of SS, TSN and TCASN in the hydrolyzates together with
electrophoretic mobility maps indicates that the high-pressure
treatment of this study accelerated protein hydrolysis compared to
ambient-pressure treatment.
Abstract: Multi-agent system approach has proven to be an effective and appropriate abstraction level to construct whole models of a diversity of biological problems, integrating aspects which can be found both in "micro" and "macro" approaches when modeling this type of phenomena. Taking into account these considerations, this paper presents the important computational characteristics to be gathered into a novel bioinformatics framework built upon a multiagent architecture. The version of the tool presented herein allows studying and exploring complex problems belonging principally to structural biology, such as protein folding. The bioinformatics framework is used as a virtual laboratory to explore a minimalist model of protein folding as a test case. In order to show the laboratory concept of the platform as well as its flexibility and adaptability, we studied the folding of two particular sequences, one of 45-mer and another of 64-mer, both described by an HP model (only hydrophobic and polar residues) and coarse grained 2D-square lattice. According to the discussion section of this piece of work, these two sequences were chosen as breaking points towards the platform, in order to determine the tools to be created or improved in such a way to overcome the needs of a particular computation and analysis of a given tough sequence. The backwards philosophy herein is that the continuous studying of sequences provides itself important points to be added into the platform, to any time improve its efficiency, as is demonstrated herein.
Abstract: Perspective of food security in 21 century showed
shortage of food that production is faced to vital problem. Food
security strategy is applied longtime method to assess required food.
Meanwhile, nanotechnology revolution changes the world face.
Nanotechnology is adequate method utilize of its characteristics to
decrease environmental problems and possible further access to food
for small farmers. This article will show impact of production and
adoption of nanocrops on food security. Population is researchers of
agricultural research center of Esfahan province. The results of study
show that there was a relationship between uses, conversion,
distribution, and production of nanocrops, operative human
resources, operative circumstance, and constrains of usage of
nanocrops and food security. Multivariate regression analysis by
enter model shows that operative circumstance, use, production and
constrains of usage of nanocrops had positive impact on food security
and they determine in four steps 20 percent of it.
Abstract: As a result of urbanization, the unpredictable growth of industry and transport, production of chemicals, military activities, etc. the concentration of anthropogenic toxicants spread in nature exceeds all the permissible standards. Most dangerous among these contaminants are organic compounds having great persistence, bioaccumulation, and toxicity along with our awareness of their prominent occurrence in the environment and food chain. Among natural ecological tools, plants still occupying above 40% of the world land, until recently, were considered as organisms having only a limited ecological potential, accumulating in plant biomass and partially volatilizing contaminants of different structure. However, analysis of experimental data of the last two decades revealed the essential role of plants in environment remediation due to ability to carry out intracellular degradation processes leading to partial or complete decomposition of carbon skeleton of different structure contaminants. Though, phytoremediation technologies still are in research and development, their various applications have been successfully used. The paper aims to analyze mechanisms of organic contaminants uptake and detoxification in plants, being the less studied issue in evaluation and exploration of plants potential for environment remediation.
Abstract: This research was conducted for the first time at the
southeastern coasts of the Caspian Sea in order to evaluate the
performance of osteichthyes cooperatives through production (catch)
function. Using one of the indirect valuation methods in this research,
contributory factors in catch were identified and were inserted into
the function as independent variables. In order to carry out this
research, the performance of 25 Osteichthyes catching cooperatives
in the utilization year of 2009 which were involved in fishing in
Miankale wildlife refuge region. The contributory factors in catch
were divided into groups of economic, ecological and biological
factors. In the mentioned function, catch rate of the cooperative were
inserted into as the dependant variable and fourteen partial variables
in terms of nine general variables as independent variables. Finally,
after function estimation, seven variables were rendered significant at
99 percent reliably level. The results of the function estimation
indicated that human resource (fisherman quantity) had the greatest
positive effect on catch rate with an influence coefficient of 1.7 while
weather conditions had the greatest negative effect on the catch rate
of cooperatives with an influence coefficient of -2.07. Moreover,
factors like member's share, experience and fisherman training and
fishing effort played the main roles in the catch rate of cooperative
with influence coefficients of 0.81, 0.5 and 0.21, respectively.
Abstract: Influence of octane and benzene on plant cell
ultrastructure and enzymes of basic metabolism, such as nitrogen
assimilation and energy generation have been studied. Different
plants: perennial ryegrass (Lolium perenne) and alfalfa (Medicago
sativa); crops- maize (Zea mays L.) and bean (Phaseolus vulgaris);
shrubs – privet (Ligustrum sempervirens) and trifoliate orange
(Poncirus trifoliate); trees - poplar (Populus deltoides) and white
mulberry (Morus alba L.) were exposed to hydrocarbons of different
concentrations (1, 10 and 100 mM). Destructive changes in bean and
maize leaves cells ultrastructure under the influence of benzene
vapour were revealed at the level of photosynthetic and energy
generation subcellular organells. Different deviations at the level of
subcellular organelles structure and distribution were observed in
alfalfa and ryegrass root cells under the influence of benzene and
octane, absorbed through roots. The level of destructive changes is
concentration dependent. Benzene at low 1 and 10 mM concentration
caused the increase in glutamate dehydrogenase (GDH) activity in
maize roots and leaves and in poplar and mulberry shoots, though to
higher extent in case of lower, 1mM concentration. The induction
was more intensive in plant roots. The highest tested 100mM
concentration of benzene was inhibitory to the enzyme in all plants.
Octane caused induction of GDH in all grassy plants at all tested
concentrations; however the rate of induction decreased parallel to
increase of the hydrocarbon concentration. Octane at concentration 1
mM caused induction of GDH in privet, trifoliate and white mulberry
shoots. The highest, 100mM octane was characterized by inhibitory
effect to GDH activity in all plants. Octane had inductive effect on
malate dehydrogenase in almost all plants and tested concentrations,
indicating the intensification of Trycarboxylic Acid Cycle.
The data could be suggested for elaboration of criteria for plant
selection for phytoremediation of oil hydrocarbons contaminated
soils.
Abstract: The purpose of research was to know the role of
immunogenic protein of 49 kDa from V.alginolyticus which capable
to initiate molecule expression of MHC Class II in receptor of
Cromileptes altivelis. The method used was in vivo experimental
research through testing of immunogenic protein 49 kDa from
V.alginolyticus at Cromileptes altivelis (size of 250 - 300 grams)
using 3 times booster by injecting an immunogenic protein in a
intramuscular manner. Response of expressed MHC molecule was
shown using immunocytochemistry method and SEM. Results
indicated that adhesin V.alginolyticus 49 kDa which have
immunogenic character could trigger expression of MHC class II on
receptor of grouper and has been proven by staining using
immunocytochemistry and SEM with labeling using antibody anti
MHC (anti mouse). This visible expression based on binding between
epitopes antigen and antibody anti MHC in the receptor. Using
immunocytochemistry, intracellular response of MHC to in vivo
induction of immunogenic adhesin from V.alginolyticus was shown.
Abstract: Lycopene, which can be extracted from plants and is
very popular for fruit intake, is restricted for healthy food development
due to its high price. On the other hand, it will get great safety
concerns, especially in the food or cosmetic application, if the raw
material of lycopene is produced by chemical synthesis. In this
project, we provide a key technology to bridge the limitation as
mentioned above. Based on the abundant bioresources of BCRC
(Bioresource Collection and Research Center, Taiwan), a promising
lycopene output will be anticipated by the introduction of fermentation
technology along with industry-related core energy. Our results
showed that addition of tween 80(0.2%) and span 20 produced higher
amount of lycopene. And piperidine, when was added at 48hr to the
cultivation medium, could promote lycopene excretion effectively
also.
Abstract: Migration in breast cancer cell wound healing assay
had been studied using image fractal dimension analysis. The
migration of MDA-MB-231 cells (highly motile) in a wound healing
assay was captured using time-lapse phase contrast video microscopy
and compared to MDA-MB-468 cell migration (moderately motile).
The Higuchi fractal method was used to compute the fractal
dimension of the image intensity fluctuation along a single pixel
width region parallel to the wound. The near-wound region fractal
dimension was found to decrease three times faster in the MDA-MB-
231 cells initially as compared to the less cancerous MDA-MB-468
cells. The inner region fractal dimension was found to be fairly
constant for both cell types in time and suggests a wound influence
range of about 15 cell layer. The box-counting fractal dimension
method was also used to study region of interest (ROI). The MDAMB-
468 ROI area fractal dimension was found to decrease
continuously up to 7 hours. The MDA-MB-231 ROI area fractal
dimension was found to increase and is consistent with the behavior
of a HGF-treated MDA-MB-231 wound healing assay posted in the
public domain. A fractal dimension based capacity index has been
formulated to quantify the invasiveness of the MDA-MB-231 cells in
the perpendicular-to-wound direction. Our results suggest that image
intensity fluctuation fractal dimension analysis can be used as a tool
to quantify cell migration in terms of cancer severity and treatment
responses.
Abstract: This paper reports optimization of characteristics of bioballistic transformation of spring soft wheat (Triticum aestivum L. cultivar Raduga) and getting of transgenic plants, carrying pea lectin gene. This gene will let to create new associative wheat symbiosis with nodule bacteria of field pea, which has growth encouraging, fungistatic and other useful characteristics.
Abstract: The research study evaluated the performance of
irrigation system by using special scientific tools like Remote
Sensing and GIS technology, so that proper measurements could be
taken for the sustainable agriculture and water management.
Different performance evaluation parameters had been calculated for
the purposed data was gathered from field investigation and different
government and private organizations. According to the calculations,
organic matter ranges from 0.19% (low value) to 0.76% (high value).
In flat irrigation system for wheat yield ranges from 3347.16 to
5260.39 kg/ha, while the total water applied to wheat crop ranges
from 252.94 to 279.19 mm and WUE ranges from 13.07 to 18.37
kg/ha/mm. For rice yield ranges from 3347.47 to 5433.07 kg/ha with
total water supplied to rice crop ranges from 764.71 to 978.15 mm
and WUE ranges from 3.49 to 5.71 kg/ha/mm. Similarly, in raised
bed system wheat yield ranges from 4569.13 to 6008.60 kg/ha, total
water supplied ranges from 158.87 to 185.09 mm and WUE ranges
from 27.20 to 33.54 kg/ha/mm while in rice crop, yield ranges from
5285.04 to 6716.69 kg/ha, total water supplied ranges from 600.72 to
755.06 mm and WUE ranges from 6.41 to 10.05 kg/ha/mm. Almost
51.3% water saving is observed in bed irrigation system as compared
to flat system. Less water supplied to beds is more affective as its
WUE value is higher than flat system where more water is supplied
in both the seasons. Similarly, RWS values show that maximum
water deficit while minimum area is getting adequate water supply.
Greater yield is recorded in bed system as plant per square meter is
more in bed system in comparison of flat system Thus, the integration
of GIS tools to regularly compute performance indices could provide
irrigation managers with the means for managing efficiently the
irrigation system.
Abstract: β-Glucosidase is an important enzyme for production
of ethanol from lignocellulose. With hydrolytic activity on
cellooligosaccharides, especially cellobiose, β-glucosidase removes
product inhibitory effect on cellulases and forms fermentable sugars.
In this study, β-glucosidase encoding gene (BGL1) from traditional
starter yeast Saccharomycosis fibuligera BMQ908 was cloned and
expressed in Pichia pastoris. BGL1 of S. fibuligera BMQ 908 shared
98% nucleotide homology with the closest GenBank sequence
(M22475) but identity in amino-acid sequences of catalytic domains.
Recombinant plasmid pPICZαA/BGL1 containing the sequence
encoding BGL1 mature protein and α-factor secretion signal was
constructed and transformed into methylotrophic yeast P. pastoris by
electroporation. The recombinant strain produced single extracellular
protein with molecular weight of 120 kDa and cellobiase activity of
60 IU/ml. The optimum pH of the recombinant β-glucosidase was 5.0
and the optimum temperature was 50°C.
Abstract: Candida spp. are common and aggressive pathogens.
Because of the growing resistance of Candida spp. to current
antifungals, novel targets, found in Candida spp. but not in humans
or other flora, have to be identified. The alternative oxidase (AOX)
is one such possibility. This enzyme is insensitive to cyanide, but is
sensitive to compounds such as salicylhydroxamic acid (SHAM),
disulfiram and n-alkyl gallates. The growth Candida albicans was
inhibited by SHAM (Ki = 9-15 mM) and cyanide (Ki = 2-4 mM),
albeit to differing extents. The rate of O2 uptake was inhibited by
less than 10% by 25 mM SHAM and by about 90% by 250 μM
KCN. Although SHAM substantially inhibited the growth of C.
albicans, it is unlikely that the inhibition of AOX was the cause.
Salicylhydroxamic acid is used therapeutically in the treatment of
urinary tract infections and urolithiasis, but it also has some potential
in the treatment of C. albicans infection.
Abstract: Experiments with pumpkin-rowanberry marmalade
candies were carried out at the Faculty of Food Technology of the
Latvia University of Agriculture. The objective of this investigation
was to evaluate the quality changes of pumpkin-rowanberry
marmalade candies packed in different packaging materials during
the storage of 15 weeks, and to find the most suitable packaging
material for prolongation of low sugar marmalade candies shelf-life.
An active packaging in combination with modified atmosphere
(MAP, CO2 100%) was examined and compared with traditional
packaging in air ambiance. Polymer Multibarrier 60 and paper bags
were used. Influence of iron based oxygen absorber in sachets of 500
cc obtained from Mitsubishi Gas Chemical Europe Ageless® on the
marmalade candies’ quality was tested during shelf life. Samples of
80±5 g were packaged in polymer pouches (110 mm x 110 mm),
hermetically sealed by MULTIVAC C300 vacuum chamber machine,
and stored in a room temperature +21±0.5 °C. The physiochemical
properties –moisture content, hardness, aw, pH, changes of
atmosphere content (CO2 and O2), ascorbic acid, total carotenoids,
total phenols in headspace of packs, and microbial conditions were
analysed before packaging and in the 1st, 3rd , 5th, 8th, 11th and 15th
weeks of storage.
Abstract: The experiment was conducted to evaluate
digestibility quantities of protein in Canola Meals (CMs) between
caecectomised and intact adult Rhode Island Red (RIR) cockerels
with using conventional addition method (CAM) for 7 d: a 4-d
adaptation and a 3-d experiment period on the basis of a completely
randomized design with 4 replicates. Results indicated that
caecectomy decreased (P
Abstract: The paper presents a part of the results obtained in a
complex research project on Romanian Grey Steppe breed, owner of
some remarkable qualities such as hardiness, longevity, adaptability,
special resistance to ban weather and diseases and included in the
genetic fund (G.D. no. 822/2008.) from Romania.
Following the researches effectuated, we identified alleles of six
loci, codifying the six types of major milk proteins: alpha-casein S1
(α S1-cz); beta-casein (β-cz); kappa-casein (K-cz); beta-lactoglobulin
(β-lg); alpha-lactalbumin (α-la) and alpha-casein S2 (α S2-cz). In
system αS1-cz allele αs1-Cn B has the highest frequency (0.700), in
system β-cz allele β-Cn A2 ( 0.550 ), in system K-cz allele k-CnA2 (
0.583 ) and heterozygote genotype AB ( 0.416 ) and BB (0.375), in
system β-lg allele β-lgA1 has the highest frequency (0.542 ) and
heterozygote genotype AB ( 0.500 ), in system α-la there is
monomorphism for allele α-la B and similarly in system αS2-cz for
allele αs2-Cn A.
The milk analysis by the isoelectric focalization technique (I.E.F.)
allowed the identification of a new allele for locus αS1-casein, for two
of the individuals under analysis, namely allele called αS1-casein
IRV. When experiments were repeated, we noticed that this is not a
proteolysis band and it really was a new allele that has not been
registered in the specialized literature so far. We identified two
heterozygote individuals, carriers of this allele, namely: BIRV and
CIRV. This discovery is extremely important if focus is laid on the
national genetic patrimony.
Abstract: Proteins levels produced by bacteria may be increased
in stressful surroundings, such as in the presence of antibiotics. It
appears that many antimicrobial agents or antibiotics, when used at
low concentrations, have in common the ability to activate or repress
gene transcription, which is distinct from their inhibitory effect.
There have been comparatively few studies on the potential of
antibiotics or natural compounds in nature as a specific chemical
signal that can trigger a variety of biological functions. Therefore,
this study was focusing on the effect of essential oils from
Cymbopogon flexuosus and C. nardus in regulating proteins
production by Bacillus subtilis ATCC 21332. The Minimum
Inhibition Concentrations (MICs) of both essential oils on B. subtilis
were determined by using microdilution assay, resulting 0.2% and
1.56% for each C. flexuosus and C. nardus subsequently. The
bacteria were further exposed to each essential oils at concentration
of 0.01XMIC for 2 days. The proteins were then isolated and
analyzed by sodium dodecyl sulfate polyacrylamide gel
electrophoresis (SDS-PAGE). Protein profile showed that a band
with approximate size of 250 kD was appeared for the treated
bacteria with essential oils. Thus, Bacillus subtilis ATCC 21332 in
stressful condition with the presence of essential oils at low
concentration could induce the protein production.
Abstract: Within the framework of a method of the information
theory it is offered statistics and probabilistic model for definition of
cause-and-effect relations in the coupled multicomponent
subsystems. The quantitative parameter which is defined through
conditional and unconditional entropy functions is introduced. The
method is applied to the analysis of the experimental data on
dynamics of change of the chemical elements composition of plants
organs (roots, reproductive organs, leafs and stems). Experiment is
directed on studying of temporal processes of primary soil formation
and their connection with redistribution dynamics of chemical
elements in plant organs. This statistics and probabilistic model
allows also quantitatively and unambiguously to specify the
directions of the information streams on plant organs.
Abstract: To investigate effect of salt stress on Chlorophyll
fluorescence four cultivars (fong,star,chamran and kharchia) of wheat
(Triticum aestivum) plants subjected to salinity levels ( control,8,12
and 16 dsm-1 ) from one week after emergence to the end of stem
elongation under greenhouse condition . results showed that quantum
yield of photosystem II from light adopted leaves (ΦPSII),
Photochemical quenching (qP) ,quantum yield of dark adopted leaves
(fv/fm) and non photochemical quenching (NPq) were affected by
salt stress . Salinity levels affected photosynthetic rate. Star and fong
cultivars showed minimum and maximum levels of photosynthetic
rate in respectively. Minimum photosynthetic rate differences
between levels of salinity were shown in Kharchia. Shoot dry matter
of all cultivars decreased by increasing salinity levels. Results
showed that non photochemical quenching by salinity levels attribute
to the decreases in shoot dry matter.
Abstract: In this study, we used a two-stage process and
potassium hydroxide (KOH) to transform waste biomass (rice straw)
into activated carbon and then evaluated the adsorption capacity of the
waste for removing carbofuran from an aqueous solution. Activated
carbon was fast and effective for the removal of carbofuran because of
its high surface area. The native and carbofuran-loaded adsorbents
were characterized by elemental analysis. Different adsorption
parameters, such as the initial carbofuran concentration, contact time,
temperature and pH for carbofuran adsorption, were studied using a
batch system. This study demonstrates that rice straw can be very
effective in the adsorption of carbofuran from bodies of water.