Abstract: Electrospinning is a broadly used technology to obtain
polymeric nanofibers ranging from several micrometers down to
several hundred nanometers for a wide range of applications. It offers
unique capabilities to produce nanofibers with controllable porous
structure. With smaller pores and higher surface area than regular
fibers, electrospun fibers have been successfully applied in various
fields, such as, nanocatalysis, tissue engineering scaffolds, protective
clothing, filtration, biomedical, pharmaceutical, optical electronics,
healthcare, biotechnology, defense and security, and environmental
engineering. In this study, polyurethane nanofibers were obtained
under different electrospinning parameters. Fiber morphology and
diameter distribution were investigated in order to understand them
as a function of process parameters.
Abstract: MinC plays an important role in bacterial cell division
system by inhibiting FtsZ assembly. However, the molecular
mechanism of the action is poorly understood. E. coli MinC Nterminus
domain was purified and crystallized using 1.4 M sodium
citrate pH 6.5 as a precipitant. X-ray diffraction data was collected
and processed to 2.3 Å from a native crystal. The crystal belonged to
space group P212121, with the unit cell parameters a = 52.7, b = 54.0,
c = 64.7 Å. Assuming the presence of two molecules in the
asymmetric unit, the Matthews coefficient value is 1.94 Å3 Da-1,
which corresponds to a solvent content of 36.5%. The overall
structure of MinCN is observed as a dimer form through anti-parallel
ß-strand interaction.
Abstract: research goal was to determine the expression levels cDNA of brain embrio at gestation days 10 (GD-10). The Electroforesis DNA results showed that GAPDH, Fibronectin1, Ncam1, Tenascin, Vimentin, Neurofilament heavy, Neurofilament medium and Neurofilament low were 447 bp, 462 bp, 293 bp. 416 bp, 327 bp, 301 bp, 398 bp and 289 bp. Result of real-time RT-PCR on brain Embryo at gestation days 10 showed that the expression of copy gen Fibronectin 36 copies, Ncam 21,708 copies; Tenascin 24,505 copies; Vimentin 538,554 copies; Neurofilament heavy 2,419 copies; Neurofilament medium 92,928 copies; Neurofilament low 125,809 copies. Vimentin expressed gene copies is very high compared with other gene copies. This condition are caused by Vimentin, that contribute to proliferate of brain development. The vimentin role to cell proliferation of brain.
Abstract: Nothing that an effective cure for infertility happens
when we can find a unique solution, a great deal of study has been
done in this field and this is a hot research subject for to days study.
So we could analyze the men-s seaman and find out about fertility
and infertility and from this find a true cure for this, since this will be
a non invasive and low risk procedure, it will be greatly welcomed.
In this research, the procedure has been based on few Algorithms
enhancement and segmentation of images which has been done on
the images taken from microscope in different fertility institution and
have obtained a suitable result from the computer images which in
turn help us to distinguish these sperms from fluids and its
surroundings.
Abstract: Acid rain occurs when sulphur dioxide (SO2) and
nitrogen oxides (Nox) gases react in the atmosphere with water,
oxygen, and other chemicals to form various acidic compounds. The
result is a mild solution of sulfuric acid and nitric acid. Soil has a
greater buffering capacity than aquatic systems. However excessive
amount of acids introduced by acid rains may disturb the entire soil
chemistry. Acidity and harmful action of toxic elements damage
vegetation while susceptible microbial species are eliminated. In
present study, the effects of simulated sulphuric acid and nitric acid
rains were investigated on crop Glycine max. The effect of acid rain
on change in soil fertility was detected in which pH of control sample
was 6.5 and pH of 1%H2SO4 and 1%HNO3 were 3.5. Nitrogen nitrate
in soil was high in 1% HNO3 treated soil & Control sample.
Ammonium nitrogen in soil was low in 1% HNO3 & H2SO4 treated
soil. Ammonium nitrogen was medium in control and other samples.
The effect of acid rain on seed germination on 3rd day of germination
control sample growth was 7 cm, 0.1% HNO3 was 8cm, and 0.001%
HNO3 & 0.001% H2SO4 was 6cm each. On 10th day fungal growth
was observed in 1% and 0.1%H2SO4 concentrations, when all plants
were dead. The effect of acid rain on crop productivity was
investigated on 3rd day roots were developed in plants. On12th day
Glycine max showed more growth in 0.1% HNO3, 0.001% HNO3 and
0.001% H2SO4 treated plants growth were same as compare to control
plants. On 20th day development of discoloration of plant pigments
were observed on acid treated plants leaves. On 38th day, 0.1, 0.001%
HNO3 and 0.1, 0.001% H2SO4 treated plants and control plants were
showing flower growth. On 42th day, acid treated Glycine max variety
and control plants were showed seeds on plants. In Glycine max
variety 0.1, 0.001% H2SO4, 0.1, 0.001% HNO3 treated plants were
dead on 46th day and fungal growth was observed. The toxicological
study was carried out on Glycine max plants exposed to 1% HNO3
cells were damaged more than 1% H2SO4. Leaf sections exposed to
0.001% HNO3 & H2SO4 showed less damaged of cells and
pigmentation observed in entire slide when compare with control
plant. The soil analysis was done to find microorganisms in HNO3 &
H2SO4 treated Glycine max and control plants. No microorganism
growth was observed in 1% HNO3 & H2SO4 but control plant showed
microbial growth.
Abstract: Clinically, chickens showed progressively developed
clinical signs represented by sever cyanosis of the comb and wattles
with hemorrhage at the shanks, depression, and ruffling feathers with
loss of appetite and high daily mortalities. The ducks showed severe
neurological signs as torticollus, seizures and inability to stand with
mild signs of diarrhea and depression. Grossly, chickens showed
hemorrhages and congestion in most of the organs particularly lung,
liver, spleen, trachea and kidney. The examined ducks showed
multiple petechial hemorrhages, multifocal hemorrhagic necrosis in
the pancreas, pulmonary edema, congestion and hemorrhage in
meninges and congestion in the skeletal muscles. Histopathology
revealed severe congestion and hemorrhages in most of the organs
particularly lung, liver and kidney. Microscopic erosive tracheitis,
sever pulmonary congestion and perivascular oedema and lymphogranulocytic
pneumonia were constant. The liver showed hepatocyts
necrosis and lympho-granulocytic infiltration. The kidney showed
renal tubular necrosis and diffuse congestion. Multifocal, neuronal
necrosis, hemorrhages, multifocal glial nodules, lympho- histiocytic
perivascular cuffing, and occasional neuronophagia were observed in
the cerebrum. Other organs showed moderate changes.
Abstract: The effects of chitosan, a biodegradable polymer,
were studied in Grammatophyllum speciosum protocorm-like bodies
(PLBs) in vitro culture. The chitosan concentration of 0, 5, 10, 15,
20, 25, 50 or 100 mg/l were supplemented in half-strength Murashige
and Skoog (1/2 MS) liquid or on agar media containing 2% (w/v)
sucrose. The results showed that liquid medium supplemented with
15 mg/l chitosan showed the highest relative growth rate (7-fold
increase) of PLBs. On 1/2 MS agar medium supplemented with 25
mg/l chitosan gave the highest relative growth rate (4-fold increase).
The relative growth rate of G. speciosum PLBs on agar medium was
significantly lower than that in liquid medium. Moreover, chitosan,
supplemented to agar medium promoted shoot formation but not
rooting. However, supplementation at too high a level, such as 100
mg/l can inhibit growth and kill PLBs.
Abstract: The aim of this study was to screen for
microorganism that able to utilize 3-N-trimethylamino-1-propanol
(homocholine) as a sole source of carbon and nitrogen. The aerobic
degradation of homocholine has been found by a gram-positive
Rhodococcus sp. bacterium isolated from soil. The isolate was
identified as Rhodococcus sp. strain A4 based on the phenotypic
features, physiologic and biochemical characteristics, and
phylogenetic analysis. The cells of the isolated strain grown on both
basal-TMAP and nutrient agar medium displayed elementary
branching mycelia fragmented into irregular rod and coccoid
elements. Comparative 16S rDNA sequencing studies indicated that
the strain A4 falls into the Rhodococcus erythropolis subclade and
forms a monophyletic group with the type-strains of R. opacus, and
R. wratislaviensis. Metabolites analysis by capillary electrophoresis,
fast atom bombardment-mass spectrometry, and gas
chromatography- mass spectrometry, showed trimethylamine (TMA)
as the major metabolite beside β-alanine betaine and
trimethylaminopropionaldehyde. Therefore, the possible degradation
pathway of trimethylamino propanol in the isolated strain is through
consequence oxidation of alcohol group (-OH) to aldehyde (-CHO)
and acid (-COOH), and thereafter the cleavage of β-alanine betaine
C-N bonds yielded trimethylamine and alkyl chain.
Abstract: Eukaryotic protein-coding genes are interrupted by spliceosomal introns, which are removed from the RNA transcripts before translation into a protein. The exon-intron structures of different eukaryotic species are quite different from each other, and the evolution of such structures raises many questions. We try to address some of these questions using statistical analysis of whole genomes. We go through all the protein-coding genes in a genome and study correlations between the net length of all the exons in a gene, the number of the exons, and the average length of an exon. We also take average values of these features for each chromosome and study correlations between those averages on the chromosomal level. Our data show universal features of exon-intron structures common to animals, plants, and protists (specifically, Arabidopsis thaliana, Caenorhabditis elegans, Drosophila melanogaster, Cryptococcus neoformans, Homo sapiens, Mus musculus, Oryza sativa, and Plasmodium falciparum). We have verified linear correlation between the number of exons in a gene and the length of a protein coded by the gene, while the protein length increases in proportion to the number of exons. On the other hand, the average length of an exon always decreases with the number of exons. Finally, chromosome clustering based on average chromosome properties and parameters of linear regression between the number of exons in a gene and the net length of those exons demonstrates that these average chromosome properties are genome-specific features.
Abstract: The present work was conducted to find out the effect
of biofertilizer formulated with four species of bacteria (two species
of Azotobacter and two species of Lysobacter) and zinc sulphate.
Field experiments with mustard plant were conducted to study the
effectiveness of soil application of zinc sulphate and biofertilizer at
0, 10, 20, 30, 40, 50 days after sowing. Plant height and condition of
plant was found to be increased significantly using a mixture of
biofertilizer and zinc sulphate than other treatments after 40 days
sowing. Three treatments were also used in this field experiment such
as bacteria only, zinc sulphate only and mixture of biofertilizer and
zinc sulphate. The treatment using a mixture of zinc sulphate and
biofertilizer had the best yield (4688.008 kg/ha) within 50 days of
sowing and performed better than other treatments. Field experiment
using zinc sulphate only was second best yield (3380.75Kg/ha) and
biofertilizer only treatment gave (2639.04kg/ha).
Abstract: The problem on the conservation programme of the Royal Thai Navy Sea Turtle Nursery, Phang-nga Province, Thailand is high mortality rate of juvenile green sea turtle (Cheloniamydas) on nursing period. So, during May to October 2012, postmortem examinations of juvenile green sea turtle were performed to determine the causes of dead. Fresh tissues of postmortem of 15 juvenile green sea turtles (1-3 months old) were investigated using paraffin section technique. The results showed normal ultrastructure of all tissue organs. These instances reviewed the health and stability of the environments in which juvenile green sea turtles live and concern for the survival rate. The present article also provides guidance for a review of the biology, guidelines for appropriate postmortem tissue, normal histology and sampling collection and procedures. The data also provides information for conservation of this endangered species in term of acknowledging and encouraging people to protect the animals and their habitats in nature.
Abstract: Aroma forming volatiles are important components of
fermented beverages. The aim of current research is to evaluate the
volatile compounds and phenolic compounds of commercial ciders.
Volatile aroma compounds and TPC of seven commercial ciders
were determined. Extraction of aroma compounds was performed
using solid phase microextraction (DVB/Car/PDMS fibre). Analysis
of volatile aroma compounds was made using a Perkin Elmer Clarus
500 GC/MS. Total phenol content (TPC) was determined according
to the Folin-Ciocalteu spectrophotometric method and results were
expressed as gallic acid equivalents. The highest volatile compounds
were in apple ciders with pear flavor. The highest TPC and lower
content of volatile compounds were detected in French ciders.
Abstract: The objective of this study was to develop and compare alternative prediction equations of lean meat proportion (LMP) of lamb carcasses. Forty (40) male lambs, 22 of Churra Galega Bragançana Portuguese local breed and 18 of Suffolk breed were used. Lambs were slaughtered, and carcasses weighed approximately 30 min later in order to obtain hot carcass weight (HCW). After cooling at 4º C for 24-h a set of seventeen carcass measurements was recorded. The left side of carcasses was dissected into muscle, subcutaneous fat, inter-muscular fat, bone, and remainder (major blood vessels, ligaments, tendons, and thick connective tissue sheets associated with muscles), and the LMP was evaluated as the dissected muscle percentage. Prediction equations of LMP were developed, and fitting quality was evaluated through the coefficient of determination of estimation (R2 e) and standard error of estimate (SEE). Models validation was performed by k-fold crossvalidation and the coefficient of determination of prediction (R2 p) and standard error of prediction (SEP) were computed. The BT2 measurement was the best single predictor and accounted for 37.8% of the LMP variation with a SEP of 2.30%. The prediction of LMP of lamb carcasses can be based simple models, using as predictors the HCW and one fat thickness measurement.
Abstract: In order to study floristic and molecular classification
of common wild wheat (Triticum boeoticum Boiss.), an analysis was
conducted on populations of the Triticum boeoticum collected from
different regions of Iran. Considering all floristic compositions of
habitats, six floristic groups (syntaxa) within the populations were
identified. A high level of variation of T. boeoticum also detected
using SSR markers. Our results showed that molecular method
confirmed the grouping of floristic method. In other word, the results
from our study indicate that floristic classification are still useful,
efficient, and economic tools for characterizing the amount and
distribution of genetic variation in natural populations of T.
boeoticum. Nevertheless, molecular markers appear as useful and
complementary techniques for identification and for evaluation of
genetic diversity in studied populations.
Abstract: Every 2-3 years the influenza B virus serves
epidemics. Neuraminidase (NA) is an important target for influenza
drug design. Although, oseltamivir, an oral neuraminidase drug, has
been shown good inhibitory efficiency against wild-type of influenza
B virus, the lower susceptibility to the R152K mutation has been
reported. Better understanding of oseltamivir efficiency and
resistance toward the influenza B NA wild-type and R152K mutant,
respectively, could be useful for rational drug design. Here, two
complex systems of wild-type and R152K NAs with oseltamivir
bound were studied using molecular dynamics (MD) simulations.
Based on 5-ns MD simulation, the loss of notable hydrogen bond and
decrease in per-residue decomposition energy from the mutated
residue K152 contributed to drug compared to those of R152 in wildtype
were found to be a primary source of high-level of oseltamivir
resistance due to the R152K mutation.
Abstract: This study was designed to determine effect of
supplemented tomato pomace and fobrolytic enzyme on egg
production and egg quality. A total of 40 CP brown laying hens (95
week old) were used in completely randomized design in 2x2
factorial arrangement with or without enzyme supplementation. Four
dietary treatments included: Control (C), Fibrolytic enzyme (FE),
10% Tomato pomace (TP), and Fibrolytic enzyme + 10 % Tomato
pomace (FE+TP). Each of the four dietary treatments was fed up to
30 days (10 birds/treatment). Live performance, egg production, egg
weight and quality were determined for whole period. Dietary
treatments had no effect (P>0.05) on live performance, egg weight,
yolk color, and egg production. Therefore, laying hens fed diets with
fibrolytic enzyme were significantly (P
Abstract: The problem of agricultural-soil pollution is closely
linked to the production of ecologically pure foodstuffs and to human health. An important task, therefore, is to rehabilitate agricultural
soils with the help of state-of-the-art biotechnologies, based on the use of metal-accumulating plants. In this work, on the basis of
literature data and the results of prior research from this laboratory, plants were selected for which the growing technology is well
developed and which are widespread locally: sugar sorghum (Sorghum saccharatum), sudangrass (Sorghum sudanense (Piper.)
Stapf.), and sunflower (Helianthus annuus L.). I report on laboratory
experiments designed to study the influence of synthetic indole-3-
acetic acid and the extracellular indole-3-acetic acid released by the
plant-growth-promoting rhizobacterium Azospirillum brasilense Sp245 on growth of and arsenic accumulation by these plants.
Abstract: The present study aimed to investigate whether
chlorophyll meter readings (SPAD) can be used as criterion of singleplant
selection in maize breeding. Experimentation was performed at
the ultra-low density of 0.74 plants/m2 in order the potential yield per
plant to be fully expressed. R-31 honeycomb experiments were
conducted in three different areas in Greece (Thessaloniki, Giannitsa
and Florina) using 30 inbred lines at well-watered and water-stressed
conditions during the 2012 growing season. The chlorophyll meter
readings had higher rates at dry conditions, except location of
Giannitsa where differences were not significant. Genotypes of
highest chlorophyll meter readings were consistent across areas,
emphasizing on the character’s stability. A positive correlation
between the chlorophyll meter readings and grain yield was
strengthening over time and culminated at the physiological maturity
stage. There was a clear sign that the chlorophyll meter readings has
the potential to be used for the selection of stress-adaptive genotypes
and may permit modern maize to be grown at wider range of
environments addressing the climate change scenarios.
Abstract: Attempts were made to identify anuran glial cells. They were found as nervous tissue resident. Having stage dependent morphotype changes, whereby, appeared as an ovoid to oval in resting state and amoeboid mrophotypes in activated state, stained fairly with methylene blue and take up Pelikane blue 10% aqueous solution, as well as having the ability to phagocytize heat killed Staphylococcus aureus. They were delineated from the migrating peripheral monocytes by morphotypic and morphometeric differences. Such criteria were consistence with glial cells. Thus, the anuran glial cells are being identified in the frog Rana ridibunda Pallas 1771 and this animal can be of use as a simple model for the immunobiology of glial cells.
Abstract: This research was conducted to determine responses
of chickpeas to drought in different periods (early period, late period,
no-irrigation, two times irrigation as control). The trial was made in
“Randomized Complete Block Design" with three replications on
2010 and 2011 years in Konya-Turkey. Genotypes were consisted
from 7 lines of ICARDA, 2 certified lines and 1 local population. The
results showed that; as means of years and genotypes, early period
stress showed highest (207.47 kg da-1) seed yield and it was followed
by control (202.33 kg da-1), late period (144.64 kg da-1) and normal
(106.93 kg da-1) stress applications. The genotypes were affected too
much by drought and, the lowest seed was taken from non-irrigated
plots. As the means of years and stress applications, the highest
(196.01 kg da-1) yield was taken from genotype 22255. The reason of
yield variation could be derived from different responses of
genotypes to drought.