Abstract: This research was conducted to determine effects of
plant densities on seed yield and some agricultural characteristics of
pea variety- Jofs in Konya ecological conditions during 2012
vegetation period. The trial was set up according to “Randomized
Blocks Design” with three replications. The material “Jofs” pea
variety was subjected to 3-row spaces (30, 40 and 50 cm) and 3-row
distances (5, 10 and 15 cm). According to the results, difference was
shown statistically for the effects of row spaces and row distances on
seed yield. The highest seed yield was 2582.1 kg ha-1 on 30 cm of
row spaces while 2562.2 kg ha-1 on 15 cm of distances.
Consequently, the optimum planting density was determined as 30 x
15 cm for Jofs pea variety growing in Konya.
Abstract: The aims of study were investigation on chemical
composition essential oil and the effect of extract of Coronilla varia
on antimicrobial and cytotoxicity activity. The essential oils of
Coronilla varia is obtained by hydrodistillation and analyzed by
(GC/MS) for determining their chemical composition and
identification of their components. Antibacterial activity of plant
extract was determined by disc diffusion method and anticancer
activity measured by MTT assay. The major components in essential
oil were Caryophyllene Oxide (60.19%), Alphacadinol (4.13%) and
Homoadantaneca Robexylic Acid (3.31%). The extracts from
Coronilla varia had interesting activity against Proteus mirabilis in
the concentration of 700 μg/disc and did not show any activity
against Staphylococus aureus, Bacillus subtillis, Klebsiella
pneumonia and Entrobacter cloacae. The positive control,
Ampicillin, Chloramphenicol and Cenphalothin had shown zone of
inhibition resistant all bacteria. The ethanol extract of Corohilla varia
inhibited on MCF7 cell lines. IC50 0.6(mg/ml) was the optimum
concentration of extract from Coronilla varia inhibition of cell line
growth. The MCF7 cancer cell line and Proteus mirabilis were more
sensitive to Coronilla varia ethanol extract.
Abstract: Moringa oleifera is a nutritious vegetable tree with
varieties of potential uses, as almost every part of the Moringa
oleifera tree can be used for food. This study was conducted in Oyo
State, Nigeria, to find out the level of acceptability of Moringa
oleifera diversified products among rural and urban dwellers.
Purposive sampling was used to select two local governments’ areas.
Stratified sampling technique was also used to select one community
each from rural and urban areas while snowball sampling technique
was used to select ten respondents each from the two communities,
making a total number of forty respondents. Data were analyzed
using frequencies, percentages, Chi-square, Pearson Product Moment
Correlation and regression analysis. Result from the study revealed
that majority of the respondents (80%) fell within the age range of
20-49 years and 55% of them were male, 55% were married, 70% of
them were Christians, 80% of them had tertiary education. The result
also showed that 85% were aware of the Moringa plant and (65%) of
them have consumed Moringa oleifera and the perception statements
on the benefits of Moringa oleifera indicated that (52.5%) of the
respondents rated Moringa oleifera to be favorable, most of them had
high acceptability for Moringa egusi soup, Moringa tea, Moringa pap
and yam pottage with Moringa. The result of the hypotheses testing
showed that there is a significant relationship between sex of the
respondents and acceptability of the diversified Moringa oleifera
products (x2=6.465, p = 0.011). There is also a significant
relationship between family size of the respondents level of
acceptability of the Moringa oleifera products (r = 0.327, p = 0.040).
Based on the level of acceptability of Moringa oleifera diversified
products; the plant is of great economic importance to the populace.
Therefore, there should be more public awareness through the media
to enlighten people on the beneficial effects of Moringa oleifera.
Abstract: An experiment was conducted to determine the effect
of pollination on seed quality of rapeseed in Chitwan, Nepal during
2012-2013. The experiment was designed in Randomized Complete
Block with four replications and five treatments. The rapeseed plots
were caged with mosquito nets at 10% flowering except natural
pollination. Two-framed colonies of Apis mellifera L. and Apis
cerana F. were introduced separately for pollination, and control plot
caged without pollinators. The highest germination percent was
observed on Apis cerana F. pollinated plot seeds (90.50%
germination) followed by Apis mellifera L. pollinated plots (87.25 %)
and lowest on control plots (42.00% germination) seeds. Similarly,
seed test weight of Apis cerana F. pollinated plots (3.22 gm/ 1000
seed) and Apis mellifera L. pollinated plots (2.93 gm/1000 seed) were
and lowest on control plots (2.26 gm/ 1000 seed) recorded. Likewise,
oil content was recorded highest on pollinated by Apis cerana F.
(36.1%) followed by pollinated by Apis mellifera L. (35.4%) and
lowest on control plots (32.8%). This study clearly indicated
pollination increases the seed quality of rapeseed and therefore,
management of honeybee is necessary for producing higher quality of
rapeseed under Chitwan condition.
Abstract: Aim of this work was to study the genetic basis for oil
accumulation in olive fruit via tracking DGAT2 (Diacylglycerol
acyltransferase type-2) gene in three Egyptian Origen Olive cultivars
namely Toffahi, Hamed and Maraki using molecular marker
techniques and bioinformatics tools. Results illustrate that, firstly:
specific genomic band of Maraki cultivars was identified as DGAT2
(Diacylglycerol acyltransferase type-2) and identical for this gene in
Olea europaea with 100% of similarity. Secondly, differential
genomic band of Maraki cultivars which produced from RAPD
fingerprinting technique reflected predicted distinguished sequence
which identified as DGAT2 (Diacylglycerol acyltransferase type-2)
in Fragaria vesca subsp. Vesca with 76% of sequential similarity.
Third and finally, specific genomic specific band of Hamed cultivars
was identified as two fragments, 1- Olea europaea cultivar Koroneiki
diacylglycerol acyltransferase type 2 mRNA, complete cds with two
matches regions with 99% or 2- Predicted: Fragaria vesca subsp.
vesca diacylglycerol O-acyltransferase 2-like (LOC101313050),
mRNA with 86 % of similarity.
Abstract: The objective of this study was to determine effect of
dietary essential oil (EO) compounds, which contained
cinnamaldehyde, eugenol, peppermint, coriander, cumin, lemongrass,
and an organic carrier on feed intake, milk composition, and rumen
fermentation of dairy cows during heat exposure. Thirty-two Holstein
cows (days in milk= 60 ± 5) were assigned to one of two treatment
groups: a Control and EO fed. The experiment lasted 28 days. Dry
matter intake (DMI) was measured daily while and milk production
was measured weekly. Our result showed that DMI and milk yield
was decreased (P < 0.01) in control cows relative to EO cows.
Furthermore, supplementation with EO was associated with a
decrease in the molar proportion of propionate (P < 0.05) and
increase (P < 0.05) in acetate to propionate ratio. In conclusion, EO
supplementations in diets can be useful nutritional modification to
alleviate for the decrease DMI and milk production during heat
exposure in lactating dairy cows.
Abstract: Domestic goats (Capra hircus) are extremely diverse
species and principal animal genetic resource of the developing
world. These facilitate a persistent supply of meat, milk, fibre, and
skin and are considered as important revenue generators in small
pastoral environments. This study aimed to fingerprint β-LG gene at
PCR-RFLP level in native Saudi goat breeds (Ardi, Habsi and Harri)
in an attempt to have a preliminary image of β-LG genotypic patterns
in Saudi breeds as compared to other foreign breeds such as Indian
and Egyptian. Also, the Phylogenetic analysis was done to investigate
evolutionary trends and similarities among the caprine β-LG gene
with that of the other domestic specie, viz. cow, buffalo and sheep.
Blood samples were collected from 300 animals (100 for each breed)
and genomic DNA was extracted. A fragment of the β-LG gene
(427bp) was amplified using specific primers. Subsequent digestion
with Sac II restriction endonuclease revealed two alleles (A and B)
and three different banding patterns or genotypes i.e. AA, AB and
BB. The statistical analysis showed a general trend that β-LG AA
genotype had higher milk yield than β-LG AB and β-LG BB
genotypes. Nucleotide sequencing of the selected β-LG fragments
was done and submitted to GenBank NCBI (Accession No.
KJ544248, KJ588275, KJ588276, KJ783455, KJ783456 and
KJ874959). Phylogenetic analysis on the basis of nucleotide
sequences of native Saudi goats indicated evolutional similarity with
the GenBank reference sequences of goat, Bubalus bubalis and Bos
taurus. However, the origin of sheep which is the most closely
related from the evolutionary point of view, was located some
distance away.
Abstract: In recent decades with the development of technology
and lack of food sources, sewage sludge in production of human
foods is inevitable. Various sources of municipal and industrial
sewage sludge that is produced can provide the requirement of plant
nutrients. Soils in arid, semi-arid climate of central Iran that most
affected by water drainage, iron and zinc deficiencies, using of
sewage sludge is helpful. Therefore, the aim of this study is
investigation of sewage sludge and manure application on Ni, Pb and
Cd uptake by Savory. An experiment in a randomized complete block
design with three replications was performed. Sewage sludge
treatments consisted of four levels, control, 15, 30, 80 tons per
hectares; the manure was used in four levels of control, 20, 40 and 80
tons per hectare. Results showed that the wet and dry weights was not
affected by sewage sludge using, while, manure has significant effect
on them. The effect of sewage sludge on the cadmium and lead
concentrations were significant. Interactions of sewage sludge and
manure on dry weight values were not significant. Compare mean
analysis showed that increasing the amount of sewage sludge had no
significant effect on cadmium concentration and it reduced when
sewage sludge usage increased. This is probably due to increased
plant growth and reduced concentrations of these elements in the
plant.
Abstract: Essential oils are expensive phytochemicals produced
and extracted from specific species belonging to particular families in
the plant kingdom. In the United Arab Emirates country (UAE), is
located in the arid region of the world, nine species, from the
Lamiaceae family, having the capability to produce therapeutic grade
essential oils. These species include; Mentha spicata, Ocimum
forskolei, Salvia macrosiphon, Salvia aegyptiaca, Salvia macilenta,
Salvia spinosa, Teucrium polium, Teucrium stocksianum and Zataria
multiflora. Although, such potential species are indigenous to the
UAE, however, there are almost no studies available to investigate
the chemical composition and the quality of the extracted essential
oils under the UAE climatological conditions. Therefore, great
attention has to be given to such valuable natural resources, through
conducting highly supported research projects, tailored to the UAE
conditions, and investigating different extraction techniques,
including the application of the latest available technologies, such as
superficial fluid CO2. This is crucially needed; in order to accomplish
the greatest possibilities in the medicinal field, specifically in the
discovery of new therapeutic chemotypes, as well as, to achieve the
sustainability of this natural resource in the country.
Abstract: The present study involved analysis of certain
characteristics of the perennial ryegrass (Lolium perenne L.)
genotypes collected from the natural flora of Ankara, and explores a
correlation among them. In order to evaluate the plants for breeding
purpose as per Turkey's environmental conditions, the perennial
ryegrass plants were collected from natural pasture of Ankara in 2004
and were utilized for the study. Seeds of the collected plants were
sown in pots and seedlings were prepared in a greenhouse. In 2005,
the seedlings were transplanted at 50 × 50 cm2 intervals in
Randomized Complete Blocks Design in an experimental field. In
2007 and 2008, data were recorded from the observations and
measurements of 568 perennial ryegrasses. The plant characteristics,
which were investigated, included re-growth time in spring, color,
density, growth habit, tendency to form inflorescence, time of
inflorescence, plant height, length of upper internode, spike length,
leaf length, leaf width, leaf area, leaf shape, number of spikelets per
spike, seed yield per spike and 1000 grain weight and the correlation
analyses were made using this data. Correlation coefficients were
estimated between all paired combinations of the studied traits. The
yield components exhibited varying trends of association among
themselves. Seed yield per spike showed significant and positive
association with the number of spikelets per spike, 1000 grain weight,
plant height, length of upper internode, spike length, leaf length, leaf
width, leaf area and color, but significant and negative association
with the growth habit and re-growth time in spring.
Abstract: Pesticides and drugs used in agriculture and veterinary
medicine may end up in aquatic environments and bioaccumulate in
the food chain, thus causing serious problems for fauna and human
health. For determination of the toxic effects of atrazine herbicide on
Caspian kutum, Rutilus frisii kutum larvae, the 96-h LC50 of atrazine
was measured for newly hatched larvae as 18.53 ppm. Toxicity of
atrazine herbicide on Caspian kutum larvae was investigated using
concentrations: 9.25ppm, 4.62 ppm and 2.31 ppm for 7 days.
Comparison of the length, weight and condition factor showed that
no significant differences between atrazine exposed and control
groups. The concentration of Na+, K+, Ca2+, Mg2+ and Cl- in whole
body of larvae in control and atrazine exposure groups were
measured and the results showed that concentrations of all these ions
is higher in atrazine exposure group than control group. It is obvious
from this study that atrazine negatively affects osmoregulation
process and changes ion compositions of the body even at sublethal
concentration and acute exposure but have no effects on growth
parameters of the body.
Abstract: Food contamination occurs during post process
handling. This leads to spoilage and growth of pathogenic
microorganisms in the food, thereby reducing its shelf life or
spreading of food borne diseases. Several methods are tried and one
of which is use of antimicrobial packaging. Here, papain, a protease
enzyme, is covalently immobilized with the help of glutarldehyde on
polyurethane and used as a food wrap to protect food from microbial
contamination. Covalent immobilization of papain was achieved at a
pH of 7.4; temperature of 4°C; glutaraldehyde concentration of 0.5%;
incubation time of 24h; and 50mg of papain. The formation of -C=Nobserved
in the Fourier transform infrared spectrum confirmed the
immobilization of the enzyme on the polymer. Immobilized enzyme
retained higher activity than the native free enzyme. The modified
polyurethane showed better reduction of Staphylococcus aureus
biofilm than bare polymer film (eight folds reduction in live colonies,
two times reduction in protein and 6 times reduction in
carbohydrates). The efficacy of this was studied by wrapping it over
S. aureus contaminated cottage cheese (paneer) and cheese and
stored at a temperature of 4°C for 7days. The modified film reduced
the bacterial contamination by eight folds when compared to the bare
film. FTIR also indicated reduction in lipids, sugars and proteins in
the biofilm.
Abstract: The liver is the strongest regenerating organ of the
organism, and even with 2/3 surgically removed, it can regenerate
completely. Hence liver cirrhosis may only develop when the
regenerating system is off.
We present the results of a comparative study of structural and
functional characteristics of rat liver tissue under the conditions of
toxic liver cirrhosis development, induced by carbon tetrachloride,
and its prevention/treatment by natural compounds with antioxidant
and immune stimulating action. Studies were made on Wister rats,
weighing 120~140 g. Grape seeds extracts, separately and in
combination with well-known anticirrhotic drug ursodeoxycholic
acid (Urdoxa), have demonstrated effectiveness in prevention of liver
cirrhosis development and its treatment.
Abstract: The aim of this study is to determine the effect of age
and physiological status on progesterone and energy metabolism of
Ouled Djellal (O.D) breed ewes. 40 healthy ewes were divided into
two groups, primiparous and multiparous, with 20 ewes in each
group. The body weights (BW) (Kg) were 46.6 ± 4.20 and 59.2 ±
3.02, and consuming less 25 to 30% of their basal energetic
requirements. The values of serum glucose, triglycerides and
cholesterol were lower in pregnant than in non-pregnant ewes. The
high to very high significant differences were found during the 15th
week of pregnancy for glycaemia and triglyceridemia respectively.
Concerning serum progesterone, a very highly significant difference
(p
Abstract: Drying behavior of blanched sweet potato in a cabinet
dryer using different five air temperatures (40-80°C) and ten sweet
potato varieties sliced to 5mm thickness were investigated. The
drying data were fitted to eight models. The Modified Henderson and
Pabis model gave the best fit to the experimental moisture ratio data
obtained during the drying of all the varieties while Newton (Lewis)
and Wang and Singh models gave the least fit. The values of Deff
obtained for Bophelo variety (1.27 x 10-9 to 1.77 x 10-9 m2/s) was
the least while that of S191 (1.93 x 10-9 to 2.47 x 10-9 m2/s) was the
highest which indicates that moisture diffusivity in sweet potato is
affected by the genetic factor. Activation energy values ranged from
0.27-6.54 kJ/mol. The lower activation energy indicates that drying
of sweet potato slices requires less energy and is hence a cost and
energy saving method. The drying behavior of blanched sweet potato
was investigated in a cabinet dryer. Drying time decreased
considerably with increase in hot air temperature. Out of the eight
models fitted, the Modified Henderson and Pabis model gave the best
fit to the experimental moisture ratio data on all the varieties while
Newton, Wang and Singh models gave the least. The lower activation
energy (0.27 - 6.54 kJ/mol) obtained indicates that drying of sweet
potato slices requires less energy and is hence a cost and energy
saving method.
Abstract: The postharvest quality management of tomatoes is
important to limit the amount of losses that occur due to deterioration
between harvest and consumption. This study was undertaken to
investigate the effects of pre- and postharvest integrated agrotechnologies,
involving greenhouse microclimate and postharvest
storage conditions, on the postharvest quality attributes of four
tomato cultivars. Tomato fruit firmness, colour (hue angle (h°) and
L* value), pH and total soluble solids for the cultivars Bona,
Star 9037, Star 9009 and Zeal, grown in a fan-pad evaporativelycooled
and an open-ended naturally-ventilated tunnel, were harvested
at the mature-green stage. The tomatoes were stored for 28 days
under cold storage conditions, with a temperature of 13°C and RH of
85%, and under ambient air conditions, with a temperature of 23±
2°C and RH of 52± 4%. This study has provided information on the
effect of integrated pre-harvest and postharvest agro-technologies,
involving greenhouse microclimate and postharvest storage
environment on the postharvest quality attributes of four of the
tomato cultivars in South Africa. NVT-grown tomatoes retained
better textural qualities, but ripened faster by changing from green to
red faster, although these were reduced under cold storage conditions.
FPVT-grown tomatoes had lower firmness, but ripened slowly with
higher colour attributes. With cold storage conditions, the firmness of
FPVT-grown tomatoes was maintained. Cultivar Bona firmness and
colour qualities depreciated the fastest, but it had higher TSS content
and lower pH values. Star 9009 and Star 9037 presented better
quality, by retaining higher firmness and ripening slowly, but they
had the lowest TSS contents and high pH values, especially
Star 9037. Cold storage improved the firmness of tomato cultivars
with poor textural quality and faster colour changes.
Abstract: GRF, Growth regulating factor, genes encode a novel
class of plant-specific transcription factors. The GRF proteins play a
role in the regulation of cell numbers in young and growing tissues
and may act as transcription activations in growth and development
of plants. Identification of GRF genes and their expression are
important in plants to performance of the growth and development of
various organs. In this study, to better understanding the structural
and functional differences of GRFs family, 45 GRF proteins
sequences in A. thaliana, Z. mays, O. sativa, B. napus, B. rapa, H.
vulgare and S. bicolor, have been collected and analyzed through
bioinformatics data mining. As a result, in secondary structure of
GRFs, the number of alpha helices was more than beta sheets and in
all of them QLQ domains were completely in the biggest alpha helix.
In all GRFs, QLQ and WRC domains were completely protected
except in AtGRF9. These proteins have no trans-membrane domain
and due to have nuclear localization signals act in nuclear and they
are component of unstable proteins in the test tube.
Abstract: Kigelia africana (Lam.) Benth. (Bignoniaceae) is a
reputed traditional remedy for various human ailments such as skin
diseases, microbial infections, melanoma, stomach troubles,
metabolic disorders, malaria and general pains. In spite of the fruit
being widely used for purposes related to its antibacterial and
antifungal properties, the chemical constituents associated with the
activity have not been fully identified. To elucidate the active
principles, we evaluated the antimicrobial activity of fruit extracts
and purified fractions against Staphylococcus aureus, Enterococcus
faecalis, Moraxella catarrhalis, Escherichia coli, Candida albicans
and Candida tropicalis. Shade-dried fruits were powdered and
extracted with hydroalcoholic (1:1) mixture by soaking at room
temperature for 72 h. The crude extract was further fractionated by
column chromatography, with successive elution using hexane,
dichloromethane, ethyl acetate, acetone and methanol. The
dichloromethane and ethyl acetate fractions were combined and
subjected to column chromatography to furnish a wax and oil from
the eluates of 20% and 40% ethyl acetate in hexane, respectively. The
GC-MS and GC×GC-MS results revealed that linoleic acid, linolenic
acid, palmitic acid, arachidic acid and stearic acid were the major
constituents in both oil and wax. The crude hydroalcoholic extract
exhibited the strongest activity with MICs of 0.125-0.5 mg/mL,
followed by the ethyl acetate (MICs = 0.125-1.0 mg/mL),
dichloromethane (MICs = 0.250-2.0 mg/mL), hexane (MICs = 0.25-
2.0 mg/mL), acetone (MICs = 0.5-2.0 mg/mL) and methanol (MICs =
1.0-2.0 mg/mL), whereas the wax (MICs = 2.0-4.0 mg/mL) and oil
(MICs = 4.0-8.0 mg/mL) showed poor activity. The study concludes
that synergistic interactions of chemical constituents could be
responsible for the antimicrobial activity of K. africana fruits, which
needs a more holistic approach to understand the mechanism of its
antimicrobial activity.
Abstract: A study was conducted to determine the diversity and
abundance of shorebird species habituating the mudflat area of Jeram
Beach and Remis Beach, Selangor, Peninsular Malaysia. Direct
observation technique (using binoculars and video camera) was
applied to record the presence of bird species in the sampling sites
from August 2013 until July 2014. A total of 32 species of shorebird
were recorded during both migratory and non-migratory seasons. Of
these, eleven species (48%) are migrants, six species (26%) have both
migrant and resident populations, four species (17%) are vagrants and
two species (9%) are residents. The compositions of the birds
differed significantly in all months (χ2 = 84.35, p < 0.001). There is a
significant difference in avian abundance between migratory and
non-migratory seasons (Mann-Whitney, t = 2.39, p = 0.036). The
avian abundance were differed significantly in Jeram and Remis
Beaches during migratory periods (t = 4.39, p = 0.001) but not during
non-migratory periods (t = 0.78, p = 0.456). Shorebird diversity was
also affected by tidal cycle. There is a significance difference
between high tide and low tide (Mann-Whitney, t = 78.0, p < 0.005).
Frequency of disturbance also affected the shorebird distribution
(Mann-Whitney, t = 57.0, p = 0.0134). Therefore, this study
concluded that tides and disturbances are two factors that affecting
temporal distribution of shorebird in mudflats area.
Abstract: This research aims to investigate callus induction,
somatic embryogenesis and indirect plant regeneration of Crassula
ovata (Mill.) Druce – the famous ornamental plant. Experiment no.1:
Callus induction was obtained from leaf and stem explants on
Murashige and Skoog (MS) medium supplemented with various plant
growth regulators (PGRs). Effects of different PGRs, plant
regeneration and subsequent plantlet conversion were also assessed.
Indirect plant regeneration was achieved from the callus of stem
explants by the addition of 1.5 mg/L Kinetin (KN) alone. Best shoot
induction was achieved (6.5 shoots/per explant) after 60 days. For
successful rooting, regenerated plantlets were sub-cultured on the
same MS media supplemented with 1.5 mg/L KN alone. The rooted
plantlets were acclimatized and the survival rate was 90%.
Experiment no.2: Results revealed that 0.5 mg/L 2,4-D alone and in
combination with 1.0 mg/L 6-Benzyladenine (BA) gave 89.8% callus
from the stem explants as compared to leaf explants. Callus
proliferation and somatic embryo formation were also evaluated by
‘Double Staining Method’ and different stages of somatic
embryogenesis were revealed by scanning electron microscope. Full
Strength MS medium produced the highest number (49.6%) of
cotyledonary stage somatic embryos (SEs). Mature cotyledonary
stage SEs developed into plantlets after 12 weeks of culture. Wellrooted
plantlets were successfully acclimatized at the survival rate of
85%. Indirectly regenerated plants did not show any detectable
variation in morphological and growth characteristics when
compared with the donor plant.