Chemical Composition of Essential Oil and in vitro Antibacterial and Anticancer Activity of the Hydroalcolic Extract from Coronilla varia
The aims of study were investigation on chemical
composition essential oil and the effect of extract of Coronilla varia
on antimicrobial and cytotoxicity activity. The essential oils of
Coronilla varia is obtained by hydrodistillation and analyzed by
(GC/MS) for determining their chemical composition and
identification of their components. Antibacterial activity of plant
extract was determined by disc diffusion method and anticancer
activity measured by MTT assay. The major components in essential
oil were Caryophyllene Oxide (60.19%), Alphacadinol (4.13%) and
Homoadantaneca Robexylic Acid (3.31%). The extracts from
Coronilla varia had interesting activity against Proteus mirabilis in
the concentration of 700 μg/disc and did not show any activity
against Staphylococus aureus, Bacillus subtillis, Klebsiella
pneumonia and Entrobacter cloacae. The positive control,
Ampicillin, Chloramphenicol and Cenphalothin had shown zone of
inhibition resistant all bacteria. The ethanol extract of Corohilla varia
inhibited on MCF7 cell lines. IC50 0.6(mg/ml) was the optimum
concentration of extract from Coronilla varia inhibition of cell line
growth. The MCF7 cancer cell line and Proteus mirabilis were more
sensitive to Coronilla varia ethanol extract.
[1] Gonzales GF, Valerio LG.2006. Medicinal plants from Peru: a review of
plants as potential agents against cancer. Anticancer Agents Med Chem;
6: 429–444.
[2] Rafter, J.J., 2002. Scientific basis of biomarkers and benefits of
functional foods for reduction of disease risk: Cancer. Br. J. Nutr., 88:
S219-S224.
[3] Madhusudan S, Middleton MR. 2005 The immerging role of DNA
repair proteins as predictive, prognostic, and therapeutics in cancer.
Cancer Treat;31:603-617.
[4] Mignogna MD, Fedele S, Lo Russo L. 2004 .The World cancer report
and the burden of oral cancer. Eur J Cancer Prev;13:139–142.
[5] Cowan MM (1999).Plant products as antimicrobial gents. Clin.Microb.,
22:564-582.
[6] Reische DW, Lillard DA, Eitenmiller RR1998. Antioxidants in food
lipids. In: Ahoh CC, Min DB (Eds.), Chemistry, Nutrition and
Biotechnology. Marcel Dekker, New York, pp. 423–448.
[7] Sokmen A, Jones BM, Erturk M,1999.Antimicrobial activity of extracts
from the cell cultures of some Turkish medicinal plants Phytotherapy
Research,13, ( 4)pages 355–357
[8] Oka Y, Elisseeva OA, Tsuboi A, Ogawa H, Tamaki H, Li H, Oji Y, Kim
EH, Soma T, Asada M, Ueda K, Maruya E, Saji H, Kishimoto T, Udaka
K, Sugiyama H. 2000 Human cytotoxic T-lymphocyte responses specific for peptides of the wild-type Wilms' tumor gene (WT1 )
product. Immunogenetics. Feb;51(2):99-107.
[9] Launert. E. Edible and Medicinal Plants. Hamlyn 1981 ISBN 0-600-
37216-2 Covers plants in Europe. a drawing of each plant, quite a bit of
interesting information.
[10] Bauer AW, Kirby MM, Sherris JC, Truck M. Antibiotic susceptibility
testing by a standardized single disk method. Am J Clin Pathol 1966; 45:
493-6.
[11] Khajeh M, Yamini Y, Bahramifar N, Sefidkon F, Pirmoradei MR
(2005).Comparison of essential oils compositions of Ferula assa-foetida
obtained by supercritical carbon dioxide extraction and hydrodistillation
methods. Food Chem., 91:639-644.
[12] Saikat Dewanjee, Anup maiti, Sanchita Das, Ranabir Sahu Subhash, C.
Mandal. 2008. In vitro Antioxidant activity of Diospyros peregrinai
fruits, Int J Pharmacol Biol Sci 2(3):135-144.
[13] Hashemi. A, Abediankenari S, Ghasemi M, Azadbakht M, Yousefzadeh
Y and Dehpour AA. 2011. The effect of Fig Tree Latex (Ficus carica) on
Stomach Cancer Line. Iranian Red Crescent Medical Journal,13(4):272-
275
[1] Gonzales GF, Valerio LG.2006. Medicinal plants from Peru: a review of
plants as potential agents against cancer. Anticancer Agents Med Chem;
6: 429–444.
[2] Rafter, J.J., 2002. Scientific basis of biomarkers and benefits of
functional foods for reduction of disease risk: Cancer. Br. J. Nutr., 88:
S219-S224.
[3] Madhusudan S, Middleton MR. 2005 The immerging role of DNA
repair proteins as predictive, prognostic, and therapeutics in cancer.
Cancer Treat;31:603-617.
[4] Mignogna MD, Fedele S, Lo Russo L. 2004 .The World cancer report
and the burden of oral cancer. Eur J Cancer Prev;13:139–142.
[5] Cowan MM (1999).Plant products as antimicrobial gents. Clin.Microb.,
22:564-582.
[6] Reische DW, Lillard DA, Eitenmiller RR1998. Antioxidants in food
lipids. In: Ahoh CC, Min DB (Eds.), Chemistry, Nutrition and
Biotechnology. Marcel Dekker, New York, pp. 423–448.
[7] Sokmen A, Jones BM, Erturk M,1999.Antimicrobial activity of extracts
from the cell cultures of some Turkish medicinal plants Phytotherapy
Research,13, ( 4)pages 355–357
[8] Oka Y, Elisseeva OA, Tsuboi A, Ogawa H, Tamaki H, Li H, Oji Y, Kim
EH, Soma T, Asada M, Ueda K, Maruya E, Saji H, Kishimoto T, Udaka
K, Sugiyama H. 2000 Human cytotoxic T-lymphocyte responses specific for peptides of the wild-type Wilms' tumor gene (WT1 )
product. Immunogenetics. Feb;51(2):99-107.
[9] Launert. E. Edible and Medicinal Plants. Hamlyn 1981 ISBN 0-600-
37216-2 Covers plants in Europe. a drawing of each plant, quite a bit of
interesting information.
[10] Bauer AW, Kirby MM, Sherris JC, Truck M. Antibiotic susceptibility
testing by a standardized single disk method. Am J Clin Pathol 1966; 45:
493-6.
[11] Khajeh M, Yamini Y, Bahramifar N, Sefidkon F, Pirmoradei MR
(2005).Comparison of essential oils compositions of Ferula assa-foetida
obtained by supercritical carbon dioxide extraction and hydrodistillation
methods. Food Chem., 91:639-644.
[12] Saikat Dewanjee, Anup maiti, Sanchita Das, Ranabir Sahu Subhash, C.
Mandal. 2008. In vitro Antioxidant activity of Diospyros peregrinai
fruits, Int J Pharmacol Biol Sci 2(3):135-144.
[13] Hashemi. A, Abediankenari S, Ghasemi M, Azadbakht M, Yousefzadeh
Y and Dehpour AA. 2011. The effect of Fig Tree Latex (Ficus carica) on
Stomach Cancer Line. Iranian Red Crescent Medical Journal,13(4):272-
275
@article{"International Journal of Biological, Life and Agricultural Sciences:70897", author = "Dehpour A. A. and Eslami B. and Rezaie S. and Hashemian S. F. and Shafie F. and Kiaie M.", title = "Chemical Composition of Essential Oil and in vitro Antibacterial and Anticancer Activity of the Hydroalcolic Extract from Coronilla varia", abstract = "The aims of study were investigation on chemical
composition essential oil and the effect of extract of Coronilla varia
on antimicrobial and cytotoxicity activity. The essential oils of
Coronilla varia is obtained by hydrodistillation and analyzed by
(GC/MS) for determining their chemical composition and
identification of their components. Antibacterial activity of plant
extract was determined by disc diffusion method and anticancer
activity measured by MTT assay. The major components in essential
oil were Caryophyllene Oxide (60.19%), Alphacadinol (4.13%) and
Homoadantaneca Robexylic Acid (3.31%). The extracts from
Coronilla varia had interesting activity against Proteus mirabilis in
the concentration of 700 μg/disc and did not show any activity
against Staphylococus aureus, Bacillus subtillis, Klebsiella
pneumonia and Entrobacter cloacae. The positive control,
Ampicillin, Chloramphenicol and Cenphalothin had shown zone of
inhibition resistant all bacteria. The ethanol extract of Corohilla varia
inhibited on MCF7 cell lines. IC50 0.6(mg/ml) was the optimum
concentration of extract from Coronilla varia inhibition of cell line
growth. The MCF7 cancer cell line and Proteus mirabilis were more
sensitive to Coronilla varia ethanol extract.", keywords = "Coronilla varia, Essential oil, Antibacterial,
Anticancer, HeLa cell line.", volume = "8", number = "12", pages = "1414-4", }