Grape Seed Extract in Prevention and Treatment of Liver Toxic Cirrhosis in Rats

The liver is the strongest regenerating organ of the
organism, and even with 2/3 surgically removed, it can regenerate
completely. Hence liver cirrhosis may only develop when the
regenerating system is off.
We present the results of a comparative study of structural and
functional characteristics of rat liver tissue under the conditions of
toxic liver cirrhosis development, induced by carbon tetrachloride,
and its prevention/treatment by natural compounds with antioxidant
and immune stimulating action. Studies were made on Wister rats,
weighing 120~140 g. Grape seeds extracts, separately and in
combination with well-known anticirrhotic drug ursodeoxycholic
acid (Urdoxa), have demonstrated effectiveness in prevention of liver
cirrhosis development and its treatment.

[1] Day C. P. “Treatment of alcoholic liver disease ” Liv. Transplant, 2007,
v. 13, p. 69-75.
[2] Emanuel A. “Role of adaptive immunity in alcoholic liver disease”
International Journal of Hepatology, v 2012, Article ID 893026, 7 pages.
[3] Detlef Schuppan and Nezam H. Afdal “Liver Cirrhosis” The Lancet Vol.
371. Issue 9615, Pages 838 - 851, 8 March 2008
[4] Badavi M., Mehrgardi F.Z., Sarkaki A., Naseri M.K., Dianat M. “Effect
of Grape Seed Extract on Lead Induced Hypertension and Heart Rate in
Rats.” J. Biol. Sci., 2008, v. 11(6), p. 882-887.
[5] Mamikonyan V. “Antioxidant influence of Vitis Vinifera Satira
Hayreniq” grape seeds Horizons in Molecular Biology 5th International
PhD Student Symposium, Gottingen, Germany, 2008, p. 84.
[6] Vivian Molina Cuevas, Yazmín Ravelo Calzado, Yohani Pérez Guerra,
Ambar Oyarzábal Yera, Sonia Jiménez Despaigne, Rosa Mas Ferreiro,
and Daisy Carbajal Quintana “Effects of Grape Seed Extract, Vitamin C,
and Vitamin E on Ethanol- and Aspirin-Induced Ulcers” Advances in
Pharmacological Sciences, Volume 2011 (2011), Article ID 740687, 6
[7] Karlen Hovnanyan, Vardan Mamikonyan, Anahit Margaryan, Kristine
Sargsyan, Margarita Hovnanyan, Maria Karagyozyan, Konstantin
Karageuzyan “Antioxidants as effective remedies at hepatotoxic action
of carbon tetrachloride” Journal of Biophysical Chemistry, Vol.5, No.1,
1-4 (2014)
[8] Trauner M, Graziadei IW. “Mechanisms of action and therapeutic
applications of ursodeoxycholic acid in chronic liver diseases” Aliment
Pharmacol Ther. 1999 Aug;13(8):979-96.
[9] S. Saksena, R.K. Tandon “Ursodeoxycholic acid in treatment of liver
diseases” Postgrad Med J. Feb 1997; 73(856): 75–80.
[10] Patrick Sharp, Jason S. Villano “The laboratory rat” second edition p
[11] Gi-Ppeum Lee, Won-Il Jeong, Da-Hee Jong, Sun-Hee Do, Tae-Hwan
Kim and Kyu-shik Jeong, “Diagnostic evaluation of carbon
tetrachloride-induced rat hepatic cirrhosis model” Anticancer Research
25, p. 1029-1038, (2005).
[12] МЕРКУЛОВ Г.А., 1969, 406 стр. (Merculov G. A., 406 pp).
[13] Valeer JD. Liver tissue examination. J Hepatol 2003;39:S43-9.
[14] Akram Eidi, Pejman Mortazavi, Masoud Ebrahim Tehrani, Ali Haeri
Rohani, Shahabaldin Safi “Hepatoprotective Effects of Pantothenic acid on Carbon Tetrachloride-induced Toxicity in rats” EXCLI Journal
2012;11:748-759 – ISSN 1611-2156 .
[15] Zachary D. Goodman “Grading and staging systems for inflammation
and fibrosis in chronic liver diseases” Journal of Hepatology, Volume
47, Issue 4, Pages 598–607, October 2007