Isolation and Identification of Diacylglycerol Acyltransferase Type- 2 (GAT2) Genes from Three Egyptian Olive Cultivars
Aim of this work was to study the genetic basis for oil
accumulation in olive fruit via tracking DGAT2 (Diacylglycerol
acyltransferase type-2) gene in three Egyptian Origen Olive cultivars
namely Toffahi, Hamed and Maraki using molecular marker
techniques and bioinformatics tools. Results illustrate that, firstly:
specific genomic band of Maraki cultivars was identified as DGAT2
(Diacylglycerol acyltransferase type-2) and identical for this gene in
Olea europaea with 100% of similarity. Secondly, differential
genomic band of Maraki cultivars which produced from RAPD
fingerprinting technique reflected predicted distinguished sequence
which identified as DGAT2 (Diacylglycerol acyltransferase type-2)
in Fragaria vesca subsp. Vesca with 76% of sequential similarity.
Third and finally, specific genomic specific band of Hamed cultivars
was identified as two fragments, 1- Olea europaea cultivar Koroneiki
diacylglycerol acyltransferase type 2 mRNA, complete cds with two
matches regions with 99% or 2- Predicted: Fragaria vesca subsp.
vesca diacylglycerol O-acyltransferase 2-like (LOC101313050),
mRNA with 86 % of similarity.
[1] S. Kumar; T. Kahlon and S. Chaudhary. A rapid screening for
adulterants in olive oil using DNA barcodes, Food Chemi. 2011,
127,1335–1341
[2] C. Consolandi, L. Palmieri, M. Severgnini, E. Maestri, N. Marmiroli, C.
Agrimonti, L. Baldoni, P. Donini, G. Bellis and B. Castiglioni. A
procedure for olive oil traceability and authenticity: DNA extraction,
multiplex PCR and LDR-universal array analysis. Eur. Food Res.
Technol. 2008, 227:1429–1438
[3] S. Pafundo, C. Agrimonti and N. Marmiroli. Traceability of plant
contribution in olive oil by amplified fragment length polymorphisms. J.
Agric. Food Chem. 2005, 53, 6995-7002.S.
[4] Pafundo, M. Busconi, C. Agrimonti, C. Fogher and M. Marmiroli..
Storage-time effects on olive oil DNA assessed by amplified fragments
length polymorphisms. Food Chem. 2010, 123:787–793
[5] E. P. Kennedy. Biosynthesis of complex lipids. Federation Proceedings.
1961, 20, 934–940.
[6] J. Browse, and C. Somerville. Glycerolipid synthesis: biochemistry and
regulation. Annu. Rev. of Plant Phys. and Plant Molec. Biol. 1991, 42,
467–506.
[7] C. Jako, A. Kumar, Y. D. Wei, J. T. Zou, D. L. Barton, E. M. Giblin, P.
S. Covello and D. C, Taylor. Seed-specific over-expression of an
Arabidopsis cDNA encoding a diacylglycerolacyltransferase enhances
seed oil content and seed weight. Plant Physi. 2001, 126, 861–874.
[8] S. C. Lung, and R. J. Weselake. Diacylglycerolacyltransferase: a key
mediator of plant triacylglycerol synthesis. Lipids. 2006, 41, 1073–1088.
[9] B. G. Banials, K. Michael, I. Makariti, A. Kourti and P. Hatzopoulos.
The olive DGAT2 gene is developmentally regulated and shares
overlapping but distinct expression patterns with DGAT1. J. of Experi.
Bot., 2011, 62 (2). 521–532.
[10] P. Hatzopoulos, G. Banilas, K. Giannoulia, F. Gazis, N. Nikoloudakis,
D. Milioni and K Haralampidis. Breeding, molecular markers and
molecular biology of the olive tree. Euro. J. of Lipid Scie. & Techno.
2002, 104, 574–586.
[11] S. Doveri and L. Baldoni. Olive. In: Kole C, ed. Genome mapping and
molecular breeding in plants, fruits and nuts, Vol. IV. Berlin,
Heidelberg: Springer-Verlag. 2007, 253–264.
[12] G. Banilas, and P. Hatzopoulos. Developmental perplexity of oil
biosynthesis gene expression in olive. In: Berti L, Maury J, eds.
Advances in olive resources. Kerala, India: Transworld Research
Network, 2009, 1–22.
[13] G. Vergari, M. Patumi and G. Fontanazza. Use of RAPD markers in the
characterization of olive germplasm. Olivae, 1996, 60:19-22.
[14] D.S. Hassawi and T. Hdeib. Genetic analysis of olive genotypes (Olea
europaea L.) using random amplified polymorphic DNA (RAPD). J.
Genet. & Breed. 2004, 58: 141-148.
[15] Y. I. Mohamed and M. Yacout. Random Amplified Polymorphic DNA
(RAPD) Analysis of Olive (Olea europaea L.) cultivars grown in North
west coast region of Egypt. Inter. J. of Envir. Engin. 2014,1 (2) 36-40.
[16] K. Giannoulia, K. Haralampids, Z. Poghosyan, D.J. Murphy and P
Hatzopoulos. Differential expression of diacylglycerol acyltransferase
(DGAT) genes in olive tissues. Biochemi. Soci. Transa. 2000, 28, 695–
697.
[17] K. D. Lardizabal, J. T. Mai, N. W. Wagner, A. Wyrick, T. Voelker and
D. J. Hawkins. DGAT2 is a new diacylglycerolacyltransferase gene
family: purification, cloning, and expression in insect cells of two
polypeptides from Mortierellaramanniana with
diacylglycerolacyltransferase activity. J. of Biolo. Chemi. 2001, 276,
38862–38869.
[18] S. Saha, B. Enugutti, S. Rajakumari and R. Rajasekharan. Cytosolic
triacylglycerol biosynthetic pathway in oilseeds: molecular cloning and
expression of peanut cytosolic diacylglyerolacyltransferase. Plant Phys.
2006, 141, 1533–1543.
[19] M. Nelson. Lopera-Barrero, A. Jayme, P. Povh, R. Ribeiro, C. P.
Gomes, B. Carolina. Jacometo and L. Tais da Silva. Comparison of
DNA extraction protocols of fish fin and larvae samples: modified salt
(NaCl) extraction. Cien. Inv. Agr. 2008, 35(1): 77-86.
[20] http://www.totallab.co
[21] http://www.ncbi.nlm.nin.gov/BLAST/
[22] http://www.Past. com
[23] www.chirimoyo. ac.uma.es/olea gen/
[1] S. Kumar; T. Kahlon and S. Chaudhary. A rapid screening for
adulterants in olive oil using DNA barcodes, Food Chemi. 2011,
127,1335–1341
[2] C. Consolandi, L. Palmieri, M. Severgnini, E. Maestri, N. Marmiroli, C.
Agrimonti, L. Baldoni, P. Donini, G. Bellis and B. Castiglioni. A
procedure for olive oil traceability and authenticity: DNA extraction,
multiplex PCR and LDR-universal array analysis. Eur. Food Res.
Technol. 2008, 227:1429–1438
[3] S. Pafundo, C. Agrimonti and N. Marmiroli. Traceability of plant
contribution in olive oil by amplified fragment length polymorphisms. J.
Agric. Food Chem. 2005, 53, 6995-7002.S.
[4] Pafundo, M. Busconi, C. Agrimonti, C. Fogher and M. Marmiroli..
Storage-time effects on olive oil DNA assessed by amplified fragments
length polymorphisms. Food Chem. 2010, 123:787–793
[5] E. P. Kennedy. Biosynthesis of complex lipids. Federation Proceedings.
1961, 20, 934–940.
[6] J. Browse, and C. Somerville. Glycerolipid synthesis: biochemistry and
regulation. Annu. Rev. of Plant Phys. and Plant Molec. Biol. 1991, 42,
467–506.
[7] C. Jako, A. Kumar, Y. D. Wei, J. T. Zou, D. L. Barton, E. M. Giblin, P.
S. Covello and D. C, Taylor. Seed-specific over-expression of an
Arabidopsis cDNA encoding a diacylglycerolacyltransferase enhances
seed oil content and seed weight. Plant Physi. 2001, 126, 861–874.
[8] S. C. Lung, and R. J. Weselake. Diacylglycerolacyltransferase: a key
mediator of plant triacylglycerol synthesis. Lipids. 2006, 41, 1073–1088.
[9] B. G. Banials, K. Michael, I. Makariti, A. Kourti and P. Hatzopoulos.
The olive DGAT2 gene is developmentally regulated and shares
overlapping but distinct expression patterns with DGAT1. J. of Experi.
Bot., 2011, 62 (2). 521–532.
[10] P. Hatzopoulos, G. Banilas, K. Giannoulia, F. Gazis, N. Nikoloudakis,
D. Milioni and K Haralampidis. Breeding, molecular markers and
molecular biology of the olive tree. Euro. J. of Lipid Scie. & Techno.
2002, 104, 574–586.
[11] S. Doveri and L. Baldoni. Olive. In: Kole C, ed. Genome mapping and
molecular breeding in plants, fruits and nuts, Vol. IV. Berlin,
Heidelberg: Springer-Verlag. 2007, 253–264.
[12] G. Banilas, and P. Hatzopoulos. Developmental perplexity of oil
biosynthesis gene expression in olive. In: Berti L, Maury J, eds.
Advances in olive resources. Kerala, India: Transworld Research
Network, 2009, 1–22.
[13] G. Vergari, M. Patumi and G. Fontanazza. Use of RAPD markers in the
characterization of olive germplasm. Olivae, 1996, 60:19-22.
[14] D.S. Hassawi and T. Hdeib. Genetic analysis of olive genotypes (Olea
europaea L.) using random amplified polymorphic DNA (RAPD). J.
Genet. & Breed. 2004, 58: 141-148.
[15] Y. I. Mohamed and M. Yacout. Random Amplified Polymorphic DNA
(RAPD) Analysis of Olive (Olea europaea L.) cultivars grown in North
west coast region of Egypt. Inter. J. of Envir. Engin. 2014,1 (2) 36-40.
[16] K. Giannoulia, K. Haralampids, Z. Poghosyan, D.J. Murphy and P
Hatzopoulos. Differential expression of diacylglycerol acyltransferase
(DGAT) genes in olive tissues. Biochemi. Soci. Transa. 2000, 28, 695–
697.
[17] K. D. Lardizabal, J. T. Mai, N. W. Wagner, A. Wyrick, T. Voelker and
D. J. Hawkins. DGAT2 is a new diacylglycerolacyltransferase gene
family: purification, cloning, and expression in insect cells of two
polypeptides from Mortierellaramanniana with
diacylglycerolacyltransferase activity. J. of Biolo. Chemi. 2001, 276,
38862–38869.
[18] S. Saha, B. Enugutti, S. Rajakumari and R. Rajasekharan. Cytosolic
triacylglycerol biosynthetic pathway in oilseeds: molecular cloning and
expression of peanut cytosolic diacylglyerolacyltransferase. Plant Phys.
2006, 141, 1533–1543.
[19] M. Nelson. Lopera-Barrero, A. Jayme, P. Povh, R. Ribeiro, C. P.
Gomes, B. Carolina. Jacometo and L. Tais da Silva. Comparison of
DNA extraction protocols of fish fin and larvae samples: modified salt
(NaCl) extraction. Cien. Inv. Agr. 2008, 35(1): 77-86.
[20] http://www.totallab.co
[21] http://www.ncbi.nlm.nin.gov/BLAST/
[22] http://www.Past. com
[23] www.chirimoyo. ac.uma.es/olea gen/
@article{"International Journal of Biological, Life and Agricultural Sciences:68595", author = "Yahia I. Mohamed and Ahmed I. Marzouk and Mohamed A. Yacout", title = "Isolation and Identification of Diacylglycerol Acyltransferase Type- 2 (GAT2) Genes from Three Egyptian Olive Cultivars", abstract = "Aim of this work was to study the genetic basis for oil
accumulation in olive fruit via tracking DGAT2 (Diacylglycerol
acyltransferase type-2) gene in three Egyptian Origen Olive cultivars
namely Toffahi, Hamed and Maraki using molecular marker
techniques and bioinformatics tools. Results illustrate that, firstly:
specific genomic band of Maraki cultivars was identified as DGAT2
(Diacylglycerol acyltransferase type-2) and identical for this gene in
Olea europaea with 100% of similarity. Secondly, differential
genomic band of Maraki cultivars which produced from RAPD
fingerprinting technique reflected predicted distinguished sequence
which identified as DGAT2 (Diacylglycerol acyltransferase type-2)
in Fragaria vesca subsp. Vesca with 76% of sequential similarity.
Third and finally, specific genomic specific band of Hamed cultivars
was identified as two fragments, 1- Olea europaea cultivar Koroneiki
diacylglycerol acyltransferase type 2 mRNA, complete cds with two
matches regions with 99% or 2- Predicted: Fragaria vesca subsp.
vesca diacylglycerol O-acyltransferase 2-like (LOC101313050),
mRNA with 86 % of similarity.
", keywords = "Olea europaea, fingerprinting, Diacylglycerol
acyltransferase type- 2 (DGAT2).", volume = "8", number = "12", pages = "1398-5", }
