Abstract: Sunflower oil with high oleic acid content is most desirable because of its high oxidative stability. Screening sunflower of high oleic acid using conventional method is laborious and time consuming. Therefore, the use of molecular markers as a screening tool is promising. The objective of this research was to evaluate SSR primers for high oleic acid content in sunflower. Two sunflower lines, 5A and PI 649855 were used as the representative of low and high oleic acid sunflowers, respectively, and thirty seven SSR markers were used to identify oleic acid content trait. The results revealing 10 SSR primers showed polymorphic between high and low oleic acid lines and thus were informative. With these primers, therefore, it is possible to identify the genetic markers associated with high oleic acid trait in sunflower genotypes.
Abstract: Measuring the effect of perinatal lead exposure on learning ability of offspring is considered as a sensitive and selective index for providing an early marker for central nervous system damage produced by this toxic metal. A total of 35 Sprague-Dawley adult rats were used to investigate the effect of lead acetate toxicity on behavioral patterns of adult female rats and learning ability of offspring. Rats were allotted into 4 groups, group one received 1g/l lead acetate (n=10), group two received 1.5g/l lead acetate (n=10), group three received 2g/l lead acetate in drinking water (n=10) and control group did not receive lead acetate (n=5) from 8th day of pregnancy till weaning of pups.
The obtained results revealed a dose dependent increase in the feeding time, drinking frequency, licking frequency, scratching frequency, licking litters, nest building and retrieving frequencies, while standing time increased significantly in rats treated with 1.5g/l lead acetate than other treated groups and control, on contrary lying time decreased gradually in a dose dependent manner. Moreover, movement activities were higher in rats treated with 1g/l lead acetate than other treated groups and control. Furthermore, time spent in closed arms was significantly lower in rats given 2g/l lead acetate than other treated groups, while, they spent significantly much time spent in open arms than other treated groups which could be attributed to occurrence of adaptation. Furthermore, number of entries in open arms was dose dependent. However, the ratio between open/closed arms revealed a significant decrease in rats treated with 2g/l lead acetate than control group.
Abstract: Paleoclimate was reconstructed by the clay mineral
assemblages of shale units of Pabdeh (Paleocene- Oligocene), Gurpi
(Upper Cretaceous), Kazhdumi (Albian-Cenomanian) and Gadvan
(Aptian-Neocomian) formations in the Bangestan anticline. To
compare with clay minerals assemblages in these formations,
selected samples also taken from available formations in drilled wells
in Ahvaz, Marun, Karanj, and Parsi oil fields. Collected samples
prepared using standard clay mineral methodology. They were
treated as normal, glycolated and heated oriented glass slides. Their
identification was made on X-Ray diffractographs. Illite % varies
from 8 to 36. Illite quantity increased from Pabdeh to Gurpi
Formation. This may be due to dominant dry climate. Kaolinite is in
range of 12-49%. Its variation style in different formations could be a
marker of climate changes from wet to dry which is supported by the
lithological changes. Chlorite (4-28%) can also be detected in those
samples without any kaolinite. Mixed layer minerals as the mixture
of illite-chlorite and illite-vermiculite-montmorillonite are varied
from 6 to 36%, decreased during Kazhdumi deposition from the base
to the top. This result may be according to decreasing of illite
leaching process. Vermiculite was also determined in very less
quantity and found in those units without kaolinite. Montmorillonite
varies from 8 to 43%, and its presence is due to terrestrial
depositional condition. Stratigraphical documents is also supported
this idea that clay mineral distribution is a function of the climate
changes. It seems, thus, the present results can be indicated a possible
procedure for ancient climate changes evaluation.
Abstract: The purpose of this study was to evaluate the use of
mucus production as a biomarker. This was done by exposing the
mussel Perna perna to various sublethal concentrations of Cu.
Mussels are effective as a bioindicator species as they accumulate Cu
in their tissues. Differences in mucus production rates were evaluated
at different Cu concentrations. The findings of this study indicate that
increasing Cu concentrations had a significant effect on the mucus
production rates over a 24 hour exposure. There were also significant
differences between the mucus production rates at different Cu
concentrations (p < 0.05). Thus, mucus is an essential detoxification
mechanism.
Abstract: Oxidative stress and overwhelming free radicals
associated with diabetes mellitus are likely to be linked with
development of certain complication such as retinopathy,
nephropathy and neuropathy. Treatment of diabetic subjects with
antioxidant may be of advantage in attenuating these complications.
Olive leaf (Oleaeuropaea), has been endowed with many beneficial
and health promoting properties mostly linked to its antioxidant
activity. This study aimed to evaluate the significance of
supplementation of Olive leaves extract (OLE) in reducing oxidative
stress, hyperglycemia and hyperlipidemia in Sterptozotocin (STZ)-
induced diabetic rats. After induction of diabetes, a significant rise in
plasma glucose, lipid profiles except High density lipoproteincholestrol
(HDLc), malondialdehyde (MDA) and significant decrease
of plasma insulin, HDLc and Plasma reduced glutathione GSH as
well as alteration in enzymatic antioxidants was observed in all
diabetic animals. During treatment of diabetic rats with 0.5g/kg body
weight of Olive leaves extract (OLE) the levels of plasma (MDA)
,(GSH), insulin, lipid profiles along with blood glucose and
erythrocyte enzymatic antioxidant enzymes were significantly
restored to establish values that were not different from normal
control rats. Untreated diabetic rats on the other hand demonstrated
persistent alterations in the oxidative stress marker (MDA), blood
glucose, insulin, lipid profiles and the antioxidant parameters. These
results demonstrate that OLE may be of advantage in inhibiting
hyperglycemia, hyperlipidemia and oxidative stress induced by
diabetes and suggest that administration of OLE may be helpful in
the prevention or at least reduced of diabetic complications
associated with oxidative stress.
Abstract: Laser Doppler flowmetry is a modern method of noninvasive
microcirculation investigation. The aim of our study was to
use this method in the examination of patients with secondary
lymphedema of the lower extremities and obliterating atherosclerosis
of lower extremities. In the analysis of the amplitude-frequency
spectrum of secondary lymphedema patients we have identified
remarkable changes. To describe the changes we used a special
amplitude rate. In both of patients groups this rate was significally
(p
Abstract: This paper describes a 3D modeling system in
Augmented Reality environment, named 3DARModeler. It can be
considered a simple version of 3D Studio Max with necessary
functions for a modeling system such as creating objects, applying
texture, adding animation, estimating real light sources and casting
shadows. The 3DARModeler introduces convenient, and effective
human-computer interaction to build 3D models by combining both
the traditional input method (mouse/keyboard) and the tangible input
method (markers). It has the ability to align a new virtual object with
the existing parts of a model. The 3DARModeler targets nontechnical
users. As such, they do not need much knowledge of
computer graphics and modeling techniques. All they have to do is
select basic objects, customize their attributes, and put them together
to build a 3D model in a simple and intuitive way as if they were
doing in the real world. Using the hierarchical modeling technique,
the users are able to group several basic objects to manage them as a
unified, complex object. The system can also connect with other 3D
systems by importing and exporting VRML/3Ds Max files. A
module of speech recognition is included in the system to provide
flexible user interfaces.
Abstract: Music Information Retrieval (MIR) and modern data mining techniques are applied to identify style markers in midi music for stylometric analysis and author attribution. Over 100 attributes are extracted from a library of 2830 songs then mined using supervised learning data mining techniques. Two attributes are identified that provide high informational gain. These attributes are then used as style markers to predict authorship. Using these style markers the authors are able to correctly distinguish songs written by the Beatles from those that were not with a precision and accuracy of over 98 per cent. The identification of these style markers as well as the architecture for this research provides a foundation for future research in musical stylometry.
Abstract: In this paper, fully developed flow and heat transfer of
viscoelastic materials in curved ducts with square cross section under
constant heat flux have been investigated. Here, staggered mesh is
used as computational grids and flow and heat transfer parameters
have been allocated in this mesh with marker and cell method.
Numerical solution of governing equations has being performed with
FTCS finite difference method. Furthermore, Criminale-Eriksen-
Filbey (CEF) constitutive equation has being used as viscoelastic
model. CEF constitutive equation is a suitable model for studying
steady shear flow of viscoelastic materials which is able to model
both effects of the first and second normal stress differences. Here, it
is shown that the first and second normal stresses differences have
noticeable and inverse effect on secondary flows intensity and mean
Nusselt number which is the main novelty of current research.
Abstract: Epstein-Barr virus (EBV) is implicated in the pathogenesis of the endemic Burkitt-s lymphoma (BL). The EBVpositive BL-derived cell lines initially maintain the original tumor phenotype of EBV infection (latency I, LatI), but most of them drift toward a lymphoblast phenotype of EBV latency III (LatIII) during in vitro culturing. The aim of the present work was to characterize the B-cell subsets in EBV-positive BL cell lines and to verify whether a particular cell subset correlates with the type of EBV infection. The phenotype analysis of two EBV-negative and eleven EBV-positive (three of LatI and eight of LatIII) BL cell lines was performed by polychromatic flow cytomery, based on expression pattern of CD19, CD10, CD38, CD27, and CD5 markers. Two cell subsets, CD19+CD10+ and CD19+CD10-, were defined in LatIII BL cell lines. In both subsets, the CD27 and CD5 cell surface expression was detected in a proportion of the cells.
Abstract: This study was designed to investigate the role of serum nitric oxide and sialic acid in the development of diabetic nephropathy as disease marker. Total 210 diabetic patients (age and sex matched) were selected followed by informed consent and divided into four groups (70 each) as I: control; II: diabetic; III: diabetic hypertensive; IV: diabetic nephropathy. The blood samples of all subjects were collected and analyzed for serum nitric oxide, sialic acid, fasting blood glucose, serum urea, creatinine, HbA1c and GFR. The BMI, systolic and diastolic blood pressures, blood glucose, HbA1c and serum sialic acid levels were high (p
Abstract: Bladder carcinoma is an important worldwide health problem. Both cystoscopy and urine cytology used in detecting bladder cancer suffer from drawbacks where cystoscopy is an invasive method and urine cytology shows low sensitivity in low grade tumors. This study validates easier and less time-consuming techniques to evaluate the value of combined use of angiogenin and clusterin in comparison and combination with voided urine cytology in the detection of bladder cancer patients. This study includes malignant (bladder cancer patients, n= 50), benign (n=20) and healthy (n=20) groups. The studied groups were subjected to cystoscopic examination, detection of bilharzial antibodies, urine cytology, and estimation of urinary angiogenin and clusterin by ELISA. The overall sensitivity and specificity were 66% and 75% for angiogenin, 70% and 82.5% for clusterin and 46% and 80% for voided urine cytology. Combined sensitivity of angiogenin and clusterin with urine cytology increased from 82 to 88%.
Abstract: This paper addresses the problem of recognizing and
interpreting the behavior of human workers in industrial
environments for the purpose of integrating humans in software
controlled manufacturing environments. In this work we propose a
generic concept in order to derive solutions for task-related manual
production applications. Thus, we are able to use a versatile concept
providing flexible components and being less restricted to a specific
problem or application. We instantiate our concept in a spot welding
scenario in which the behavior of a human worker is interpreted
when performing a welding task with a hand welding gun. We
acquire signals from inertial sensors, video cameras and triggers and
recognize atomic actions by using pose data from a marker based
video tracking system and movement data from inertial sensors.
Recognized atomic actions are analyzed on a higher evaluation level
by a finite state machine.
Abstract: Organic farmers across Saskatchewan face soil
phosphorus (P) shortages. Due to the restriction on inputs in organic
systems, farmers rely on crop rotation and naturally-occurring
arbuscular mycorrhizal fungi (AMF) for plant P supply. Crop rotation
is important for disease, pest, and weed management. Crops that are
not colonized by AMF (non-mycorrhizal) can decrease colonization
of a following crop. An experiment was performed to quantify soil P
cycling in four cropping sequences under organic management and
determine if mustard (non-mycorrhizal) was delaying the
colonization of subsequent wheat. Soils from the four cropping
sequences were measured for inorganic soil P (Pi), AMF spore
density (SD), phospholipid fatty acid analysis (PLFA, for AMF
biomarker counts), and alkaline phosphatase activity (ALPase,
related to AMF metabolic activity). Plants were measured for AMF
colonization and P content and uptake of above-ground biomass. A
lack of difference in AMF activity indicated that mustard was not
depressing colonization. Instead, AMF colonization was largely
determined by crop type and crop rotation.
Abstract: There are various kinds of medical equipment which
requires relatively accurate positional adjustments for successful
treatment. However, patients tend to move without notice during a
certain span of operations. Therefore, it is common practice that
accompanying operators adjust the focus of the equipment. In this
paper, tracking controllers for medical equipment are suggested to
replace the operators. The tracking controllers use AHRS sensor
information to recognize the movements of patients. Sensor fusion is
applied to reducing the error magnitudes through linear Kalman filters.
The image processing of optical markers is included to adjust the
accumulation errors of gyroscope sensor data especially for yaw
angles.
The tracking controller reduces the positional errors between the
current focus of a device and the target position on the body of a
patient. Since the sensing frequencies of AHRS sensors are very high
compared to the physical movements, the control performance is
satisfactory. The typical applications are, for example, ESWT or
rTMS, which have the error ranges of a few centimeters.
Abstract: Utilization of diverse germplasm is needed to enhance
the genetic diversity of cultivars. The objective of this study was to
evaluate the genetic relationships of 98 alfalfa germplasm accessions
using morphological traits and SSR markers. From the 98 tested
populations, 81 were locals originating in Europe, 17 were introduced
from USA, Australia, New Zealand and Canada. Three primers
generated 67 polymorphic bands. The average polymorphic
information content (PIC) was very high (> 0.90) over all three used
primer combinations. Cluster analysis using Unweighted Pair Group
Method with Arithmetic Means (UPGMA) and Jaccard´s coefficient
grouped the accessions into 2 major clusters with 4 sub-clusters with
no correlation between genetic and morphological diversity. The SSR
analysis clearly indicated that even with three polymorphic primers,
reliable estimation of genetic diversity could be obtained.
Abstract: In the present research, two nutraceuticals made from
red grape and walnut that showed previously to improve kidney
dysfunction were incorporated separately into functional foods' bread
made from barley and rice bran. The functional foods were evaluated
in rats in which chronic renal failure was induced through feeding
diet rich in adenine and phosphate (APD). The evaluation based on
assessing kidney function, oxidative stress, inflammatory biomarkers
and body weight gain. Results showed induction of chronic kidney
failure reflected in significant increase in plasma urea, creatinine,
malondialdehyde, tumor necrosis factor- α and low density
lipoprotein cholesterol along with significant reduction of plasma
albumin, and total antioxidant and creatinine clearance and body
weight gain on feeding APD compared to control healthy group.
Feeding the functional foods produced amelioration in the different
biochemical parameters and body weight gain indicating
improvement in kidney function.
Abstract: Many natural language expressions are ambiguous, and
need to draw on other sources of information to be interpreted.
Interpretation of the e word تعاون to be considered as a noun or a verb
depends on the presence of contextual cues. To interpret words we
need to be able to discriminate between different usages. This paper
proposes a hybrid of based- rules and a machine learning method for
tagging Arabic words. The particularity of Arabic word that may be
composed of stem, plus affixes and clitics, a small number of rules
dominate the performance (affixes include inflexional markers for
tense, gender and number/ clitics include some prepositions,
conjunctions and others). Tagging is closely related to the notion of
word class used in syntax. This method is based firstly on rules (that
considered the post-position, ending of a word, and patterns), and
then the anomaly are corrected by adopting a memory-based learning
method (MBL). The memory_based learning is an efficient method to
integrate various sources of information, and handling exceptional
data in natural language processing tasks. Secondly checking the
exceptional cases of rules and more information is made available to
the learner for treating those exceptional cases. To evaluate the
proposed method a number of experiments has been run, and in
order, to improve the importance of the various information in
learning.
Abstract: Quantitative trait loci (QTL) experiments have yielded
important biological and biochemical information necessary for
understanding the relationship between genetic markers and
quantitative traits. For many years, most QTL algorithms only
allowed one observation per genotype. Recently, there has been an
increasing demand for QTL algorithms that can accommodate more
than one observation per genotypic distribution. The Bayesian
hierarchical model is very flexible and can easily incorporate this
information into the model. Herein a methodology is presented that
uses a Bayesian hierarchical model to capture the complexity of the
data. Furthermore, the Markov chain Monte Carlo model composition
(MC3) algorithm is used to search and identify important markers. An
extensive simulation study illustrates that the method captures the
true QTL, even under nonnormal noise and up to 6 QTL.
Abstract: Formaldehyde is the illegal chemical substance used
for food preservation in fish and vegetable. It can promote
carcinogenesis. Superoxide dismutases are the important
antioxidative enzymes that catalyze the dismutation of superoxide
anion into oxygen and hydrogen peroxide. The resultant level of
oxidative stress in formaldehyde-treated lymphocytes was
investigated. The formaldehyde concentrations of 0, 20, 40, 60, 80
and 120μmol/L were treated in human lymphocytes for 12 hours.
After 12 treated hours, the superoxide dismutase activity change was
measured in formaldehyde-treated lymphocytes. The results showed
that the formaldehyde concentrations of 60, 80 and 120μmol/L
significantly decreased superoxide dismutase activities in
lymphocytes (P < 0.05). The change of superoxide dismutase
activity in formaldehyde-treated lymphocytes may be the biomarker
for detect cellular injury, such as damage to DNA, due to
formaldehyde exposure.