Abstract: In this study, polymerase chain reaction based Inter-simple sequence repeat (ISSR) from DNA fingerprinting techniques were used to investigate the genetic relationships among barley crossbreed genotypes in Turkey. It is important that selection based on the genetic base in breeding programs via ISSR, in terms of breeding time. 14 ISSR primers generated a total of 97 bands, of which 81 (83.35%) were polymorphic. The highest total resolution power (RP) value was obtained from the F2 (0.53) and M16 (0.51) primers. According to the ISSR result, the genetic similarity index changed between 0.64–095; Lane 3 with Line 6 genotypes were the closest, while Line 36 were the most distant ones. The ISSR markers were found to be promising for assessing genetic diversity in barley crossbreed genotypes.
Abstract: Sunflower oil with high oleic acid content is most desirable because of its high oxidative stability. Screening sunflower of high oleic acid using conventional method is laborious and time consuming. Therefore, the use of molecular markers as a screening tool is promising. The objective of this research was to evaluate SSR primers for high oleic acid content in sunflower. Two sunflower lines, 5A and PI 649855 were used as the representative of low and high oleic acid sunflowers, respectively, and thirty seven SSR markers were used to identify oleic acid content trait. The results revealing 10 SSR primers showed polymorphic between high and low oleic acid lines and thus were informative. With these primers, therefore, it is possible to identify the genetic markers associated with high oleic acid trait in sunflower genotypes.
Abstract: Utilization of diverse germplasm is needed to enhance
the genetic diversity of cultivars. The objective of this study was to
evaluate the genetic relationships of 98 alfalfa germplasm accessions
using morphological traits and SSR markers. From the 98 tested
populations, 81 were locals originating in Europe, 17 were introduced
from USA, Australia, New Zealand and Canada. Three primers
generated 67 polymorphic bands. The average polymorphic
information content (PIC) was very high (> 0.90) over all three used
primer combinations. Cluster analysis using Unweighted Pair Group
Method with Arithmetic Means (UPGMA) and Jaccard´s coefficient
grouped the accessions into 2 major clusters with 4 sub-clusters with
no correlation between genetic and morphological diversity. The SSR
analysis clearly indicated that even with three polymorphic primers,
reliable estimation of genetic diversity could be obtained.
Abstract: In order to study floristic and molecular classification
of common wild wheat (Triticum boeoticum Boiss.), an analysis was
conducted on populations of the Triticum boeoticum collected from
different regions of Iran. Considering all floristic compositions of
habitats, six floristic groups (syntaxa) within the populations were
identified. A high level of variation of T. boeoticum also detected
using SSR markers. Our results showed that molecular method
confirmed the grouping of floristic method. In other word, the results
from our study indicate that floristic classification are still useful,
efficient, and economic tools for characterizing the amount and
distribution of genetic variation in natural populations of T.
boeoticum. Nevertheless, molecular markers appear as useful and
complementary techniques for identification and for evaluation of
genetic diversity in studied populations.
Abstract: The project was undertaken to determine the effects of modified tissue culture protocols e.g. age of culture and hormone levels (2,4-D) in generating somaclonal variation. Moreover, the utility of molecular markers (SSR and MSAP) in sorting off types/somaclones were investigated.
Results show that somaclonal variation is in effect due to prolonged subculture and high 2,4-D concentration. The resultant variation was observed to be due to high level of methylation events specifically cytosine methylation either at the internal or external cytosine and was identified by methylation sensitive amplification polymorphism (MSAP).Simple sequence repeats (SSR) on the other hand, was able to associate a marker to a trait of interest.
These therefore, show that molecular markers can be an important tool in sorting out variation/mutants at an early stage.