Abstract: Utilization of diverse germplasm is needed to enhance
the genetic diversity of cultivars. The objective of this study was to
evaluate the genetic relationships of 98 alfalfa germplasm accessions
using morphological traits and SSR markers. From the 98 tested
populations, 81 were locals originating in Europe, 17 were introduced
from USA, Australia, New Zealand and Canada. Three primers
generated 67 polymorphic bands. The average polymorphic
information content (PIC) was very high (> 0.90) over all three used
primer combinations. Cluster analysis using Unweighted Pair Group
Method with Arithmetic Means (UPGMA) and Jaccard´s coefficient
grouped the accessions into 2 major clusters with 4 sub-clusters with
no correlation between genetic and morphological diversity. The SSR
analysis clearly indicated that even with three polymorphic primers,
reliable estimation of genetic diversity could be obtained.