Abstract: This study was conducted to determine sex
differentiation of laboratory reared Elm nymphalid (Nymphalis
polychloros Linnaeus, 1758) by examining the morphological
structure of pupal stage. Laboratory colony of elm nymphalid, reared
on pear leaves, was used to set up experiments. It was performed
with 5 replications having 8 pupae for each replication. Dorsal,
ventral and lateral parts of external morphological structures of pupae
were examined by Olympus SZX9 stereozoom microscope and
photographed. When fully grown, mature larvae wander the highest
part of the rearing cage and pupae were formed hanging by
cremaster. After completing prepupa stage about 1.5±0.3 days, they
all pupated. Pupal stage was completed at 24±1°C about 4.38±1.20
days. Pupal weights were 0.483±0.05 g in females and 0.392±0.08 g
(n=40) in males respectively. Pupal emergence rate was 95%, with
22 females and 16 males. Examinations of ventral parts of 8th, 9th,
and 10th abdominal segments revealed that anal opening were found
at 10th abdominal segment in both sexes, 3 lumps were determined at
9th abdominal segments then the specific opening structure at 8th
segment was only found on female pupae.
Abstract: In this article the problem of distributional moments estimation is considered. The new approach of moments estimation based on usage of the characteristic function is proposed. By statistical simulation technique author shows that new approach has some robust properties. For calculation of the derivatives of characteristic function there is used numerical differentiation. Obtained results confirmed that author’s idea has a certain working efficiency and it can be recommended for any statistical applications.
Abstract: In this manuscript, we produced neat electrospun poly(o-methoxyaniline) (POMA) fibers and utilized it for applying the growth of neural stem cells. The transparency and morphology of as-prepared POMA fibers was characterized by UV-visible spectroscopy and scanning electron microscopy, respectively. It was found to have no adverse effects on the long-term proliferation of the neural stem cells (NSCs), retained the ability to self-renew, and exhibit multipotentiality. Results of immunofluorescence staining studies confirmed that POMA electrospun fibers could provide a great environment for NSCs and enhance its differentiation.
Abstract: Mouse L929 fibroblastic cell line, which is widely
used in many experiment aspects, was tested for their differentiation
potency in osteogenic differentiation and adipogenic differentiation.
Human dermal fibroblasts, which their differentiation potency are
still be in confliction, also be taken in the experiment. The
differentiations were conducted by using the inducing medium
ingredients which is generally used to induce differentiation of stem
cells. By the inducing media used, L929 mouse fibroblasts
successfully underwent osteogenic differentiation and adipogenic
differentiation while human dermal fibroblasts underwent only
osteogenic differentiation but not for adipogenic differentiation.
Human dermal fibroblasts are hard to be differentiated in adipogenic
lineage and need specific proper condition for induction.
Abstract: Traditionally, Internet has provided best-effort service to every user regardless of its requirements. However, as Internet becomes universally available, users demand more bandwidth and applications require more and more resources, and interest has developed in having the Internet provide some degree of Quality of Service. Although QoS is an important issue, the question of how it will be brought into the Internet has not been solved yet. Researches, due to the rapid advances in technology are proposing new and more desirable capabilities for the next generation of IP infrastructures. But neither all applications demand the same amount of resources, nor all users are service providers. In this way, this paper is the first of a series of papers that presents an architecture as a first step to the optimization of QoS in the Internet environment as a solution to a SMSE's problem whose objective is to provide public service to internet with certain Quality of Service expectations. The service provides new business opportunities, but also presents new challenges. We have designed and implemented a scalable service framework that supports adaptive bandwidth based on user demands, and the billing based on usage and on QoS. The developed application has been evaluated and the results show that traffic limiting works at optimum and so it does exceeding bandwidth distribution. However, some considerations are done and currently research is under way in two basic areas: (i) development and testing new transfer protocols, and (ii) developing new strategies for traffic improvements based on service differentiation.
Abstract: MicroRNAs are an important class of gene expression
regulators that are involved in many biological processes including
embryogenesis. miR-125b is a conserved microRNA that is enriched
in the nervous system. We have previously reported the function of
miR-125b in neuronal differentiation of human cell lines. We also
discovered the function of miR-125b in regulating p53 in human and
zebrafish. Here we further characterize the brain defects in zebrafish
embryos injected with morpholinos against miR-125b. Our data
confirm the essential role of miR-125b in brain morphogenesis
particularly in maintaining the balance between proliferation, cell
death and differentiation. We identified lunatic fringe (lfng) as an
additional target of miR-125b in human and zebrafish and suggest
that lfng may mediate the function of miR-125b in neurogenesis.
Together, this report reveals new insights into the function of miR-
125b during neural development of zebrafish.
Abstract: The stem cells have ability to differentiated
themselves through mitotic cell division and various range of
specialized cell types. Cellular differentiation is a way by which few
specialized cell develops into more specialized.This paper studies the
fundamental problem of computational schema for an artificial neural
network based on chemical, physical and biological variables of
state. By doing this type of study system could be model for a viable
propagation of various economically important stem cells
differentiation. This paper proposes various differentiation outcomes
of artificial neural network into variety of potential specialized cells
on implementing MATLAB version 2009. A feed-forward back
propagation kind of network was created to input vector (five input
elements) with single hidden layer and one output unit in output
layer. The efficiency of neural network was done by the assessment
of results achieved from this study with that of experimental data
input and chosen target data. The propose solution for the efficiency
of artificial neural network assessed by the comparatative analysis of
“Mean Square Error" at zero epochs. There are different variables of
data in order to test the targeted results.
Abstract: Calcium is very important for communication among
the neurons. It is vital in a number of cell processes such as secretion,
cell movement, cell differentiation. To reduce the system of reactiondiffusion
equations of [Ca2+] into a single equation, two theories
have been proposed one is excess buffer approximation (EBA) other
is rapid buffer approximation (RBA). The RBA is more realistic than
the EBA as it considers both the mobile and stationary endogenous
buffers. It is valid near the mouth of the channel. In this work we have
studied the effects of different types of buffers on calcium diffusion
under RBA. The novel thing studied is the effect of sodium ions on
calcium diffusion. The model has been made realistic by considering
factors such as variable [Ca2+], [Na+] sources, sodium-calcium
exchange protein(NCX), Sarcolemmal Calcium ATPase pump. The
proposed mathematical leads to a system of partial differential equations
which has been solved numerically to study the relationships
between different parameters such as buffer concentration, buffer
disassociation rate, calcium permeability. We have used Forward
Time Centred Space (FTCS) approach to solve the system of partial
differential equations.
Abstract: The study describes chitosan membrane platform
modified with nanostructure pattern which using nanotechnology to
fabricate. The cell-substrate interaction between neuro-2a neuroblasts
cell lines and chitosan membrane (flat, nanostructure and
nanostructure pattern types) was investigated. The adhered
morphology of neuro-2a cells depends on the topography of chitosan
surface. We have found that neuro-2a showed different morphogenesis
when cells adhered on flat and nanostructure chitosan membrane. The
cell projected area of neuro-2a on flat chitosan membrane is larger
than on nanostructure chitosan membrane. In addition, neuro-2a cells
preferred to adhere on flat chitosan surface region than on
nanostructure chitosan membrane to immobilize and differentiation.
The experiment suggests surface topography can be used as a critical
mechanism to isolate group of neuro-2a to a particular rectangle area
on chitosan membrane. Our finding will provide a platform to take
patch clamp to record electrophysiological behavior about neurons in
vitro in the future.