Abstract: The importance for manipulating an incorporated
scaffold and directing cell behaviors is well appreciated for tissue
engineering. Here, we developed newly nano-topographic oxidized
silicon nanosponges capable of being various chemical modifications
to provide much insight into the fundamental biology of how cells
interact with their surrounding environment in vitro. A wet etching
technique is exerted to allow us fabricated the silicon nanosponges in a
high-throughput manner. Furthermore, various organo-silane
chemicals enabled self-assembled on the surfaces by vapor deposition.
We have found that Chinese hamster ovary (CHO) cells displayed
certain distinguishable morphogenesis, adherent responses, and
biochemical properties while cultured on these chemical modified
nano-topographic structures in compared with the planar oxidized
silicon counterparts, indicating that cell behaviors can be influenced
by certain physical characteristic derived from nano-topography in
addition to the hydrophobicity of contact surfaces crucial for cell
adhesion and spreading. Of particular, there were predominant
nano-actin punches and slender protrusions formed while cells were
cultured on the nano-topographic structures. This study shed potential
applications of these nano-topographic biomaterials for controlling
cell development in tissue engineering or basic cell biology research.
Abstract: The study describes chitosan membrane platform
modified with nanostructure pattern which using nanotechnology to
fabricate. The cell-substrate interaction between neuro-2a neuroblasts
cell lines and chitosan membrane (flat, nanostructure and
nanostructure pattern types) was investigated. The adhered
morphology of neuro-2a cells depends on the topography of chitosan
surface. We have found that neuro-2a showed different morphogenesis
when cells adhered on flat and nanostructure chitosan membrane. The
cell projected area of neuro-2a on flat chitosan membrane is larger
than on nanostructure chitosan membrane. In addition, neuro-2a cells
preferred to adhere on flat chitosan surface region than on
nanostructure chitosan membrane to immobilize and differentiation.
The experiment suggests surface topography can be used as a critical
mechanism to isolate group of neuro-2a to a particular rectangle area
on chitosan membrane. Our finding will provide a platform to take
patch clamp to record electrophysiological behavior about neurons in
vitro in the future.