Neuroblasts Micropatterning on Nanostructural Modified Chitosan Membranes

The study describes chitosan membrane platform modified with nanostructure pattern which using nanotechnology to fabricate. The cell-substrate interaction between neuro-2a neuroblasts cell lines and chitosan membrane (flat, nanostructure and nanostructure pattern types) was investigated. The adhered morphology of neuro-2a cells depends on the topography of chitosan surface. We have found that neuro-2a showed different morphogenesis when cells adhered on flat and nanostructure chitosan membrane. The cell projected area of neuro-2a on flat chitosan membrane is larger than on nanostructure chitosan membrane. In addition, neuro-2a cells preferred to adhere on flat chitosan surface region than on nanostructure chitosan membrane to immobilize and differentiation. The experiment suggests surface topography can be used as a critical mechanism to isolate group of neuro-2a to a particular rectangle area on chitosan membrane. Our finding will provide a platform to take patch clamp to record electrophysiological behavior about neurons in vitro in the future.

Authors:

• Chun-Yen Sung
• Chung-Yao Yang
• Tzu-Chun Liao
• Wen-Shiang Chen
• Chao-Min Cheng
• J. Andrew Yeh

Keywords:

• Chitosan membrane
• neuro-2a
• wet chemical etching
• solvent casting.

References:

[1] N. Li and A. Folch, "Integration of topographical and biochemical cues
by axons during growth on microfabricated 3-D substrates," Exp. Cell
Res., vol. 311, pp. 307- 316, Nov. 2005.
[2] H. G. Craighead, S. W. Turner, R. C. Davis, C. James, A. M. Perez, P. M.
St. John, M. S. Isaacson, L. Kam, W. Shain, J. N. Turner and G. Banker,
"Chemical and Topographical Surface Modification for Control of
Central Nervous System Cell Adhesion," Biomed. Microdevices, vol. 1,
pp. 49-64, Sep. 1998.
[3] M. Arnold, E. A. C. Adam, R. Glass, J. B. Mmel, W. Eck, M. Kantlehner,
H. Kessler, and J. -P. Spatz, "Induction of Cell Polarization and Migration
by a Gradient of Nanoscale Variations in Adhesive Ligand Spacing,"
Nano Lett., vol. 8, pp. 2063-2069, June 2008.
[4] B. Zhua, Q. Zhangb, Q. Lua, Y. Xub, J. Yina, J. Hub and Z. Wanga,
"Nanotopographical guidance of C6 glioma cell alignment and oriented
growth," Biomaterials, vol. 25, pp. 4215-422, Aug. 2004.
[5] M. Cecchini, G. Bumma, M. Serresi and F. Beltram, "PC12
differentiation on biopolymer nanostructures," Nanotechnology, vol.18,
pp.505103, Nov. 2007.
[6] M. Cheng, J. Deng, F. Yang, Y. Gong, N. Zhao and X. Zhang, "Study on
physical properties and nerve cell affinity of composite films from
chitosan and gelatin solutions," Biomaterials, vol. 24, pp. 2871-2880,
Aug. 2003.
[7] G. Haipeng, Z. Yinghui, L. Jianchun, G. Yandao, Z. Nanming and Z.
Xiufang, "Studies on nerve cell affinity of chitosan-derived materials," J.
Biomed. Mater. Res., vol. 52, pp. 285-95, Nov. 2000.