Differentiation Capacity of Mouse L929 Fibroblastic Cell Line Compare With Human Dermal Fibroblast
Mouse L929 fibroblastic cell line, which is widely
used in many experiment aspects, was tested for their differentiation
potency in osteogenic differentiation and adipogenic differentiation.
Human dermal fibroblasts, which their differentiation potency are
still be in confliction, also be taken in the experiment. The
differentiations were conducted by using the inducing medium
ingredients which is generally used to induce differentiation of stem
cells. By the inducing media used, L929 mouse fibroblasts
successfully underwent osteogenic differentiation and adipogenic
differentiation while human dermal fibroblasts underwent only
osteogenic differentiation but not for adipogenic differentiation.
Human dermal fibroblasts are hard to be differentiated in adipogenic
lineage and need specific proper condition for induction.
[1] R. A. Evans, et al., "TGF-beta1-mediated fibroblast-myofibroblast
terminal differentiation-the role of Smad proteins," Exp Cell Res, vol.
282, pp. 90-100, Jan 15 2003.
[2] C. Helary, et al., "Dense fibrillar collagen matrices: a model to study
myofibroblast behaviour during wound healing," Biomaterials, vol. 27,
pp. 4443-52, Sep 2006.
[3] D. Bi, et al., "Differentiation of human multipotent dermal fibroblasts
into islet-like cell clusters," BMC Cell Biol, vol. 11, p. 46, 2010.
[4] J. P. Junker, et al., "Adipogenic, chondrogenic and osteogenic
differentiation of clonally derived human dermal fibroblasts," Cells
Tissues Organs, vol. 191, pp. 105-18, 2010.
[5] S. B. Nicoll, et al., "Hyaluronidases and CD44 undergo differential
modulation during chondrogenesis," Biochem Biophys Res Commun,
vol. 292, pp. 819-25, Apr 12 2002.
[6] A. Sorisky, et al., "Evidence of adipocyte differentiation in human
orbital fibroblasts in primary culture," J Clin Endocrinol Metab, vol.
81, pp. 3428-31, Sep 1996.
[7] F. Jeney, et al., "Cytochemical studies on the fibroblast-preadipocyte
relationships in cultured fibroblast cell lines," Acta Histochem, vol.
102, pp. 381-9, Nov 2000.
[8] C. Shui and A. M. Scutt, "Mouse embryo-derived NIH3T3 fibroblasts
adopt an osteoblast-like phenotype when treated with 1alpha,25-
dihydroxyvitamin D(3) and dexamethasone in vitro," J Cell Physiol,
vol. 193, pp. 164-72, Nov 2002.
[9] F. Bretagnol, et al., "The effect of sterilization processes on the
bioadhesive properties and surface chemistry of a plasma-polymerized
polyethylene glycol film: XPS characterization and L929 cell
proliferation tests," Acta Biomater, vol. 4, pp. 1745-51, Nov 2008.
[10] M. C. Serrano, et al., "Transitory oxidative stress in L929 fibroblasts
cultured on poly(epsilon-caprolactone) films," Biomaterials, vol. 26,
pp. 5827-34, Oct 2005.
[11] R. Zange and T. Kissel, "Comparative in vitro biocompatibility testing
of polycyanoacrylates and poly(D,L-lactide-co-glycolide) using
different mouse fibroblast (L929) biocompatibility test models,"
European journal of pharmaceutics and biopharmaceutics vol. 44, pp.
149-157, 1997.
[12] G. Faria, et al., "Chlorhexidine-induced apoptosis or necrosis in L929
fibroblasts: A role for endoplasmic reticulum stress," Toxicol Appl
Pharmacol, vol. 234, pp. 256-65, Jan 15 2009.
[13] M. Nordin, et al., "Effects of exposure period of acetylsalicylic acid,
paracetamol and isopropanol on L929 cytotoxicity," Toxicol In Vitro,
vol. 5, pp. 449-50, 1991.
[14] T. Taniguchi, et al., "Hydrogen peroxide-induced arachidonic acid
release in L929 cells; roles of Src, protein kinase C and cytosolic
phospholipase A2alpha," Eur J Pharmacol, vol. 546, pp. 1-10, Sep 28
2006.
[15] B. Roelofs, et al., "Acute activation of glucose uptake by glucose
deprivation in L929 fibroblast cells," Biochimie, vol. 88, pp. 1941-6,
Dec 2006.
[16] M. Cartwright, et al., "The human nerve growth factor gene: structure
of the promoter region and expression in L929 fibroblasts," Brain Res
Mol Brain Res, vol. 15, pp. 67-75, Sep 1992.
[17] H. Declercq, et al., "Isolation, proliferation and differentiation of
osteoblastic cells to study cell/biomaterial interactions: comparison of
different isolation techniques and source," Biomaterials, vol. 25, pp.
757-68, Feb 2004.
[18] J. Stern and W. H. P. Lewis, "The colorimetric estimation of calcium in
serum with o-cresolphthalein complexone," Clinica Chimica Acta, vol.
2, pp. 576-580, 1957.
[19] T. J. Wu, et al., "Studies on the microspheres comprised of
reconstituted collagen and hydroxyapatite," Biomaterials, vol. 25, pp.
651-8, Feb 2004.
[20] K. Jaager and T. Neuman, "Human Dermal Fibroblasts Exhibit Delayed
Adipogenic Differentiation Compared with Mesenchymal Stem Cells,"
Stem Cells Dev, Jan 16 2011.
[1] R. A. Evans, et al., "TGF-beta1-mediated fibroblast-myofibroblast
terminal differentiation-the role of Smad proteins," Exp Cell Res, vol.
282, pp. 90-100, Jan 15 2003.
[2] C. Helary, et al., "Dense fibrillar collagen matrices: a model to study
myofibroblast behaviour during wound healing," Biomaterials, vol. 27,
pp. 4443-52, Sep 2006.
[3] D. Bi, et al., "Differentiation of human multipotent dermal fibroblasts
into islet-like cell clusters," BMC Cell Biol, vol. 11, p. 46, 2010.
[4] J. P. Junker, et al., "Adipogenic, chondrogenic and osteogenic
differentiation of clonally derived human dermal fibroblasts," Cells
Tissues Organs, vol. 191, pp. 105-18, 2010.
[5] S. B. Nicoll, et al., "Hyaluronidases and CD44 undergo differential
modulation during chondrogenesis," Biochem Biophys Res Commun,
vol. 292, pp. 819-25, Apr 12 2002.
[6] A. Sorisky, et al., "Evidence of adipocyte differentiation in human
orbital fibroblasts in primary culture," J Clin Endocrinol Metab, vol.
81, pp. 3428-31, Sep 1996.
[7] F. Jeney, et al., "Cytochemical studies on the fibroblast-preadipocyte
relationships in cultured fibroblast cell lines," Acta Histochem, vol.
102, pp. 381-9, Nov 2000.
[8] C. Shui and A. M. Scutt, "Mouse embryo-derived NIH3T3 fibroblasts
adopt an osteoblast-like phenotype when treated with 1alpha,25-
dihydroxyvitamin D(3) and dexamethasone in vitro," J Cell Physiol,
vol. 193, pp. 164-72, Nov 2002.
[9] F. Bretagnol, et al., "The effect of sterilization processes on the
bioadhesive properties and surface chemistry of a plasma-polymerized
polyethylene glycol film: XPS characterization and L929 cell
proliferation tests," Acta Biomater, vol. 4, pp. 1745-51, Nov 2008.
[10] M. C. Serrano, et al., "Transitory oxidative stress in L929 fibroblasts
cultured on poly(epsilon-caprolactone) films," Biomaterials, vol. 26,
pp. 5827-34, Oct 2005.
[11] R. Zange and T. Kissel, "Comparative in vitro biocompatibility testing
of polycyanoacrylates and poly(D,L-lactide-co-glycolide) using
different mouse fibroblast (L929) biocompatibility test models,"
European journal of pharmaceutics and biopharmaceutics vol. 44, pp.
149-157, 1997.
[12] G. Faria, et al., "Chlorhexidine-induced apoptosis or necrosis in L929
fibroblasts: A role for endoplasmic reticulum stress," Toxicol Appl
Pharmacol, vol. 234, pp. 256-65, Jan 15 2009.
[13] M. Nordin, et al., "Effects of exposure period of acetylsalicylic acid,
paracetamol and isopropanol on L929 cytotoxicity," Toxicol In Vitro,
vol. 5, pp. 449-50, 1991.
[14] T. Taniguchi, et al., "Hydrogen peroxide-induced arachidonic acid
release in L929 cells; roles of Src, protein kinase C and cytosolic
phospholipase A2alpha," Eur J Pharmacol, vol. 546, pp. 1-10, Sep 28
2006.
[15] B. Roelofs, et al., "Acute activation of glucose uptake by glucose
deprivation in L929 fibroblast cells," Biochimie, vol. 88, pp. 1941-6,
Dec 2006.
[16] M. Cartwright, et al., "The human nerve growth factor gene: structure
of the promoter region and expression in L929 fibroblasts," Brain Res
Mol Brain Res, vol. 15, pp. 67-75, Sep 1992.
[17] H. Declercq, et al., "Isolation, proliferation and differentiation of
osteoblastic cells to study cell/biomaterial interactions: comparison of
different isolation techniques and source," Biomaterials, vol. 25, pp.
757-68, Feb 2004.
[18] J. Stern and W. H. P. Lewis, "The colorimetric estimation of calcium in
serum with o-cresolphthalein complexone," Clinica Chimica Acta, vol.
2, pp. 576-580, 1957.
[19] T. J. Wu, et al., "Studies on the microspheres comprised of
reconstituted collagen and hydroxyapatite," Biomaterials, vol. 25, pp.
651-8, Feb 2004.
[20] K. Jaager and T. Neuman, "Human Dermal Fibroblasts Exhibit Delayed
Adipogenic Differentiation Compared with Mesenchymal Stem Cells,"
Stem Cells Dev, Jan 16 2011.
@article{"International Journal of Medical, Medicine and Health Sciences:63987", author = "Kasem Theerakittayakorn and Tanom Bunprasert", title = "Differentiation Capacity of Mouse L929 Fibroblastic Cell Line Compare With Human Dermal Fibroblast", abstract = "Mouse L929 fibroblastic cell line, which is widely
used in many experiment aspects, was tested for their differentiation
potency in osteogenic differentiation and adipogenic differentiation.
Human dermal fibroblasts, which their differentiation potency are
still be in confliction, also be taken in the experiment. The
differentiations were conducted by using the inducing medium
ingredients which is generally used to induce differentiation of stem
cells. By the inducing media used, L929 mouse fibroblasts
successfully underwent osteogenic differentiation and adipogenic
differentiation while human dermal fibroblasts underwent only
osteogenic differentiation but not for adipogenic differentiation.
Human dermal fibroblasts are hard to be differentiated in adipogenic
lineage and need specific proper condition for induction.", keywords = "Adipogenic differentiation, Fibroblast, Inducingmedium, Osteogenic differentiation", volume = "5", number = "2", pages = "74-4", }
