MicroRNAs are an important class of gene expression
regulators that are involved in many biological processes including
embryogenesis. miR-125b is a conserved microRNA that is enriched
in the nervous system. We have previously reported the function of
miR-125b in neuronal differentiation of human cell lines. We also
discovered the function of miR-125b in regulating p53 in human and
zebrafish. Here we further characterize the brain defects in zebrafish
embryos injected with morpholinos against miR-125b. Our data
confirm the essential role of miR-125b in brain morphogenesis
particularly in maintaining the balance between proliferation, cell
death and differentiation. We identified lunatic fringe (lfng) as an
additional target of miR-125b in human and zebrafish and suggest
that lfng may mediate the function of miR-125b in neurogenesis.
Together, this report reveals new insights into the function of miR-
125b during neural development of zebrafish.
[1] D. P. Bartel, "MicroRNAs: genomics, biogenesis, mechanism, and
function," Cell, vol. 116, pp. 281-297, 2004.
[2] B. Ason, et al., "From the Cover: Differences in vertebrate microRNA
expression," Proc.Natl.Acad.Sci.U.S.A, vol. 103, pp. 14385-14389, 2006.
[3] M. Selbach, et al., "Widespread changes in protein synthesis induced by
microRNAs," Nature, vol. 455, pp. 58-63, 2008.
[4] P. H. Olsen and V. Ambros, "The lin-4 regulatory RNA controls
developmental timing in Caenorhabditis elegans by blocking LIN-14
protein synthesis after the initiation of translation," Dev.Biol., vol. 216,
pp. 671-680, 1999.
[5] E. E. Caygill and L. A. Johnston, "Temporal regulation of metamorphic
processes in Drosophila by the let-7 and miR-125 heterochronic
microRNAs," Curr.Biol., vol. 18, pp. 943-950, 2008.
[6] E. A. Miska, et al., "Microarray analysis of microRNA expression in the
developing mammalian brain," Genome Biol., vol. 5, p. R68, 2004.
[7] L. Smirnova, et al., "Regulation of miRNA expression during neural cell
specification," Eur.J.Neurosci., vol. 21, pp. 1469-1477, 2005.
[8] L. F. Sempere, et al., "Expression profiling of mammalian microRNAs
uncovers a subset of brain-expressed microRNAs with possible roles in
murine and human neuronal differentiation," Genome Biol., vol. 5, p.
R13, 2004.
[9] E. Wienholds, et al., "MicroRNA expression in zebrafish embryonic
development," Science, vol. 309, pp. 310-311, 2005.
[10] M. T. Le, et al., "MicroRNA-125b promotes neuronal differentiation in
human cells by repressing multiple targets," Mol Cell Biol, vol. 29, pp.
5290-305, Oct 2009.
[11] M. T. Le, et al., "MicroRNA-125b is a novel negative regulator of p53,"
Genes Dev, vol. 23, pp. 862-76, Apr 1 2009.
[1] D. P. Bartel, "MicroRNAs: genomics, biogenesis, mechanism, and
function," Cell, vol. 116, pp. 281-297, 2004.
[2] B. Ason, et al., "From the Cover: Differences in vertebrate microRNA
expression," Proc.Natl.Acad.Sci.U.S.A, vol. 103, pp. 14385-14389, 2006.
[3] M. Selbach, et al., "Widespread changes in protein synthesis induced by
microRNAs," Nature, vol. 455, pp. 58-63, 2008.
[4] P. H. Olsen and V. Ambros, "The lin-4 regulatory RNA controls
developmental timing in Caenorhabditis elegans by blocking LIN-14
protein synthesis after the initiation of translation," Dev.Biol., vol. 216,
pp. 671-680, 1999.
[5] E. E. Caygill and L. A. Johnston, "Temporal regulation of metamorphic
processes in Drosophila by the let-7 and miR-125 heterochronic
microRNAs," Curr.Biol., vol. 18, pp. 943-950, 2008.
[6] E. A. Miska, et al., "Microarray analysis of microRNA expression in the
developing mammalian brain," Genome Biol., vol. 5, p. R68, 2004.
[7] L. Smirnova, et al., "Regulation of miRNA expression during neural cell
specification," Eur.J.Neurosci., vol. 21, pp. 1469-1477, 2005.
[8] L. F. Sempere, et al., "Expression profiling of mammalian microRNAs
uncovers a subset of brain-expressed microRNAs with possible roles in
murine and human neuronal differentiation," Genome Biol., vol. 5, p.
R13, 2004.
[9] E. Wienholds, et al., "MicroRNA expression in zebrafish embryonic
development," Science, vol. 309, pp. 310-311, 2005.
[10] M. T. Le, et al., "MicroRNA-125b promotes neuronal differentiation in
human cells by repressing multiple targets," Mol Cell Biol, vol. 29, pp.
5290-305, Oct 2009.
[11] M. T. Le, et al., "MicroRNA-125b is a novel negative regulator of p53,"
Genes Dev, vol. 23, pp. 862-76, Apr 1 2009.
@article{"International Journal of Biological, Life and Agricultural Sciences:58694", author = "Minh T. N. Le and Cathleen Teh and Ng Shyh-Chang and Vladimir Korzh and Harvey F. Lodish and Bing Lim", title = "Function of miR-125b in Zebrafish Neurogenesis", abstract = "MicroRNAs are an important class of gene expression
regulators that are involved in many biological processes including
embryogenesis. miR-125b is a conserved microRNA that is enriched
in the nervous system. We have previously reported the function of
miR-125b in neuronal differentiation of human cell lines. We also
discovered the function of miR-125b in regulating p53 in human and
zebrafish. Here we further characterize the brain defects in zebrafish
embryos injected with morpholinos against miR-125b. Our data
confirm the essential role of miR-125b in brain morphogenesis
particularly in maintaining the balance between proliferation, cell
death and differentiation. We identified lunatic fringe (lfng) as an
additional target of miR-125b in human and zebrafish and suggest
that lfng may mediate the function of miR-125b in neurogenesis.
Together, this report reveals new insights into the function of miR-
125b during neural development of zebrafish.", keywords = "microRNA, miR-125b, neurogenesis, zebrafish.", volume = "4", number = "2", pages = "151-6", }