Abstract: In present study, it was aimed to evaluate the gamma radiation impacts on tomato leaf miner at different biological stages. The laboratory colony of tomato leaf miner was used to set up the experiments. Different biological stages of the insects (eggs, 4th instars and pupae) were irradiated using Cobalt-60 at doses of 0 (control), 100 Gray (Gy), 200 Gy, 300 Gy and 400 Gy in Cos-44HH-N source, at dose rate of 480 Gy/h. After irradiation, the eggs were incubated until hatching; the mature larvae were reared to complete their developments. Adult emergences from irradiated pupae were also evaluated. The results showed that there were no egg hatching at all tested irradiation doses. Although, the pupal percentages of irradiated mature larvae were 54%, 15% and 8% at doses of 100 Gy, 200 Gy and 300 Gy respectively, there were no adult emergences from irradiated mature larvae. On the other hand, the adult emergences were observed from irradiated pupae, decreased as radiation doses increased along with malformed adult appearance. Male and female individuals were out crossed with laboratory reared adults. Fecundity was correlated with radiation doses.
Abstract: The olive fruit fly, Bactrocera oleae (Rossi), is an economically important and endemic pest in olive (Oleae europae) orchards in Turkey. The aim of this study was to determine olive fruit fly infestation in different olive cultivars in the laboratory. Olive fly infested fruits were collected in Çanakkale province to establish wild fly population. After having reproductive olive fly colonies, 14 olive cultivars were tested in the controlled laboratory conditions, at 23±2 °C, 65% RH and 16:8 h (light: dark) photoperiod. The olive samples from 14 different olive cultivars were collected in October 2015, in Campus of Dardanos, Çanakkale Onsekiz Mart University. Observations were carried out detecting some biological parameters such as the number of oviposition stings, active infestation, total infestation, the number of pupae and the adult emergence. The results indicated that oviposition stings were not associated with pupal yield. A few pupae were found within olive fruits which were not able to exit. Screening of the varieties suggested that less susceptible cultivar to olive fruit fly attacks was Arbequin while Gemlik-2M 2/3 showed significant susceptibility. Ovipositional preference of olive fly females and the success of larval development in different olive varieties are crucial for establishing new olive orchards to prevent high olive fruit fly infestation.
Abstract: This study was conducted to determine sex
differentiation of laboratory reared Elm nymphalid (Nymphalis
polychloros Linnaeus, 1758) by examining the morphological
structure of pupal stage. Laboratory colony of elm nymphalid, reared
on pear leaves, was used to set up experiments. It was performed
with 5 replications having 8 pupae for each replication. Dorsal,
ventral and lateral parts of external morphological structures of pupae
were examined by Olympus SZX9 stereozoom microscope and
photographed. When fully grown, mature larvae wander the highest
part of the rearing cage and pupae were formed hanging by
cremaster. After completing prepupa stage about 1.5±0.3 days, they
all pupated. Pupal stage was completed at 24±1°C about 4.38±1.20
days. Pupal weights were 0.483±0.05 g in females and 0.392±0.08 g
(n=40) in males respectively. Pupal emergence rate was 95%, with
22 females and 16 males. Examinations of ventral parts of 8th, 9th,
and 10th abdominal segments revealed that anal opening were found
at 10th abdominal segment in both sexes, 3 lumps were determined at
9th abdominal segments then the specific opening structure at 8th
segment was only found on female pupae.